Restriction map for
pFN21AB7879
(Circular) (Six-base) MAPSORT of: pFN21AB7879.seq Check: 7984 from: 1 to: 6430
pFN21AB7879 6430 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2246 2246
Size: 6430
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 1937
Size: 1836 4594
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2650 2752 4493 5877 6145 1632
Size: 1018 102 1741 1384 268 1917
Fragments arranged by size:
1917 1741 1384 1018 268 102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 5329 6306 1203
Size: 4126 977 1327
Fragments arranged by size:
4126 1327 977
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 2283 1166
Size: 1117 5313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6430
AlwNI CAG_nnn'CTG
Cuts at: 2015 2359 4173 5745 2015
Size: 344 1814 1572 2700
Fragments arranged by size:
2700 1814 1572 344
ApaLI G'TGCA_C
Cuts at: 4487 5643 4487
Size: 1156 5274
ApoI r'AATT_y
Cuts at: 1493 2214 2441 3624 3739 3965 4117 6226 1493
Size: 721 227 1183 115 226 152 2109 1697
Fragments arranged by size:
2109 1697 1183 721 227 226 152 115
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 3755 1870
Size: 73 1812 4545
Fragments arranged by size:
4545 1812 73
AvrII C'CTAG_G
Cuts at: 2249 2249
Size: 6430
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2025 2197 3751 5206 618
Size: 325 217 627 238 172 1554 1455 1842
Fragments arranged by size:
1842 1554 1455 627 325 238 217 172
BanII G_rGCy'C
Cuts at: 1391 3749 1391
Size: 2358 4072
BbeI G_GCGC'C
Cuts at: 2029 2029
Size: 6430
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2087 2509 2591 928
Size: 971 188 422 82 4767
Fragments arranged by size:
4767 971 422 188 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4621 4655 1903
Size: 34 2684 34 3678
Fragments arranged by size:
3678 2684 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BfrBI ATG'CAT
Cuts at: 2605 2605
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5041 136
Size: 107 122 71 631 605 3369 1525
Fragments arranged by size:
3369 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6425 6425
Size: 6430
BlpI GC'TnA_GC
Cuts at: 3847 3847
Size: 6430
Bme1580I G_kGCm'C
Cuts at: 1790 2045 4491 5647 1790
Size: 255 2446 1156 2573
Fragments arranged by size:
2573 2446 1156 255
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6430
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2541 3306 4161
Size: 416 53 83 399 24 1124 765 855
Cuts at: 4161 5119 442
Size: 958 1753
Fragments arranged by size:
1753 1124 958 855 765 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2262 2262
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2982 3066 3150 3234 3402 3486 3654 5090
Size: 1743 84 84 84 168 84 168 1436
Cuts at: 5090 1239
Size: 2579
Fragments arranged by size:
2579 1743 1436 168 168 84 84 84
84
Bpu10I CC'TnA_GC
Cuts at: 2492 2492
Size: 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4555 5420 5718 4555
Size: 865 298 5267
Fragments arranged by size:
5267 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5093 882
Size: 617 3594 2219
Fragments arranged by size:
3594 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4221 493
Size: 711 3017 2702
Fragments arranged by size:
3017 2702 711
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2026 4237 4619 5254
Size: 53 83 186 936 493 2211 382 635
Cuts at: 5254 275
Size: 1451
Fragments arranged by size:
2211 1451 936 635 493 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4863 5261 5535 5682 6093 4863
Size: 398 274 147 411 5200
Fragments arranged by size:
5200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 1178
Size: 30 165 30 672 30 5503
Fragments arranged by size:
5503 672 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2207 2915 1186
Size: 913 108 708 4701
Fragments arranged by size:
4701 913 708 108
BseYI C'CCAG_C
Cuts at: 2164 5633 6129 2164
Size: 3469 496 2465
Fragments arranged by size:
3469 2465 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2749 3589 1163
Size: 573 1013 840 4004
Fragments arranged by size:
4004 1013 840 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3880 4641 5669 1540
Size: 275 186 1879 761 1028 2301
Fragments arranged by size:
2301 1879 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4491 4576 5647 4491
Size: 85 1071 5274
Fragments arranged by size:
5274 1071 85
BsmI GAATG_Cn'
Cuts at: 3488 3656 3941 4034 3488
Size: 168 285 93 5884
Fragments arranged by size:
5884 285 168 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 3749 4491 4576 5647 1391
Size: 399 255 1704 742 85 1071 2174
Fragments arranged by size:
2174 1704 1071 742 399 255 85
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 3364 4319 6049 3364
Size: 955 1730 3745
Fragments arranged by size:
3745 1730 955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2246 3785 844
Size: 1402 1539 3489
Fragments arranged by size:
3489 1539 1402
BsrBI CCG'CTC
Cuts at: 1394 1823 4317 1394
Size: 429 2494 3507
Fragments arranged by size:
3507 2494 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4925 5099 65
Size: 1183 3677 174 1396
Fragments arranged by size:
3677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5074 5261 1700
Size: 110 3264 187 2869
Fragments arranged by size:
3264 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2221 96
Size: 2125 4305
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 4490 5502 4490
Size: 1012 5418
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6430
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 3014 1184
Size: 1830 4600
BstYI r'GATC_y
Cuts at: 1972 3263 3760 4127 4512 4529 5981 6067 6079
Size: 1291 497 367 385 17 1452 86 12
Cuts at: 6079 6425 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1291 497 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4939
Fragments arranged by size:
4939 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5691
Fragments arranged by size:
5691 683 56
BtsI GCAGTG_nn'
Cuts at: 2397 2737 2790 3493 3577 3946 4740 4760 2397
Size: 340 53 703 84 369 794 20 4067
Fragments arranged by size:
4067 794 703 369 340 84 53 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3223 3258 529
Size: 35 2659 35 3701
Fragments arranged by size:
3701 2659 35 35
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DraIII CAC_nnn'GTG
Cuts at: 3439 3439
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3877 4766 6395
Size: 54 1425 50 275 18 2047 889 1629
Cuts at: 6395 8
Size: 43
Fragments arranged by size:
2047 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3877 1537
Size: 275 2065 4090
Fragments arranged by size:
4090 2065 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4360 1353
Size: 3007 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5017 5391 5537 387
Size: 1064 138 3428 374 146 1280
Fragments arranged by size:
3428 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2650 2752 2982 3066 3150 3234 3402
Size: 393 1018 102 230 84 84 84 168
Cuts at: 3402 3486 3654 4493 5090 5877 6145 1239
Size: 84 168 839 597 787 268 1524
Fragments arranged by size:
1524 1018 839 787 597 393 268 230
168 168 102 84 84 84 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2253 2253
Size: 6430
EcoO109I rG'GnC_Cy
Cuts at: 2801 2969 3222 2801
Size: 168 253 6009
Fragments arranged by size:
6009 253 168
EcoRI G'AATT_C
Cuts at: 3624 3739 4117 3624
Size: 115 378 5937
Fragments arranged by size:
5937 378 115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3118 3118
Size: 6430
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 2029 5577 1678
Size: 351 3548 2531
Fragments arranged by size:
3548 2531 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2875 2907 3043 3075 3255
Size: 32 135 32 1303 32 136 32 180
Cuts at: 3255 3287 3433 3465 5071 5103 5145 5177 1373
Size: 32 146 32 1606 32 42 32 2626
Fragments arranged by size:
2626 1606 1303 180 146 136 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3774 4020 669
Size: 988 281 1836 246 3079
Fragments arranged by size:
3079 1836 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KasI G'GCGC_C
Cuts at: 2025 2025
Size: 6430
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 3313 4029 3313
Size: 716 5714
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5544 5728 1305
Size: 4239 184 2007
Fragments arranged by size:
4239 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4608
Fragments arranged by size:
4608 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2283 3117 3397 4388 4747 4906 518
Size: 648 1117 834 280 991 359 159 2042
Fragments arranged by size:
2042 1117 991 834 648 359 280 159
MspA1I CmG'CkG
Cuts at: 1606 2012 3846 4523 5671 5916 1606
Size: 406 1834 677 1148 245 2120
Fragments arranged by size:
2120 1834 1148 677 406 245
NarI GG'CG_CC
Cuts at: 2026 2026
Size: 6430
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4939
Fragments arranged by size:
4939 939 552
NdeI CA'TA_TG
Cuts at: 387 3394 387
Size: 3007 3423
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NsiI A_TGCA'T
Cuts at: 2607 2607
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 2379 3788 5333 1132
Size: 97 1150 1409 1545 2229
Fragments arranged by size:
2229 1545 1409 1150 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6430
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6430
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2801 2801
Size: 6430
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 3564 4000 3564
Size: 436 5994
PstI C_TGCA'G
Cuts at: 830 3782 830
Size: 2952 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SfcI C'TryA_G
Cuts at: 826 1045 2446 2577 3778 4913 5594 5785 826
Size: 219 1401 131 1201 1135 681 191 1471
Fragments arranged by size:
1471 1401 1201 1135 681 219 191 131
SfoI GGC'GCC
Cuts at: 2027 2027
Size: 6430
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6430
SmaI CCC'GGG
Cuts at: 1872 3757 1872
Size: 1885 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4534 5435 5697 820
Size: 197 926 2591 901 262 1553
Fragments arranged by size:
2591 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 3139 3643 3725 1517
Size: 1622 504 82 4222
Fragments arranged by size:
4222 1622 504 82
StyI C'CwwG_G
Cuts at: 513 1065 2004 2249 3869 513
Size: 552 939 245 1620 3074
Fragments arranged by size:
3074 1620 939 552 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4457 4474 4627 4812 5257 325
Size: 1255 2877 17 153 185 445 1498
Fragments arranged by size:
2877 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2221 4676 6388
Size: 274 80 33 51 1443 244 2455 1712
Cuts at: 6388 96
Size: 138
Fragments arranged by size:
2455 1712 1443 274 244 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2075 2107 2709 2741 6390 6422 2075
Size: 32 602 32 3649 32 2083
Fragments arranged by size:
3649 2083 602 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6430
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6430
XmaI C'CCGG_G
Cuts at: 1870 3755 1870
Size: 1885 4545
XmnI GAAnn'nnTTC
Cuts at: 2702 4559 2702
Size: 1857 4573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbvCI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI