Restriction map for
pFN21AB7877
(Circular) (Six-base) MAPSORT of: pFN21AB7877.seq Check: 5555 from: 1 to: 6418
pFN21AB7877 6418 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4228 278
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3761 1937
Size: 1824 4594
Acc65I G'GTAC_C
Cuts at: 2307 3739 2307
Size: 1432 4986
AclI AA'CG_TT
Cuts at: 1563 4545 4918 1563
Size: 2982 373 3063
Fragments arranged by size:
3063 2982 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3128 3204 4481 5865 6133 1632
Size: 1496 76 1277 1384 268 1917
Fragments arranged by size:
1917 1496 1384 1277 268 76
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6221
AflIII A'CryG_T
Cuts at: 1203 3398 5317 6294 1203
Size: 2195 1919 977 1327
Fragments arranged by size:
2195 1919 1327 977
AgeI A'CCGG_T
Cuts at: 5249 5249
Size: 6418
AhdI GACnn_n'nnGTC
Cuts at: 5147 5147
Size: 6418
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6418
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 6386
AlwNI CAG_nnn'CTG
Cuts at: 2080 2186 3654 4161 5733 2080
Size: 106 1468 507 1572 2765
Fragments arranged by size:
2765 1572 1468 507 106
ApaI G_GGCC'C
Cuts at: 2850 3180 2850
Size: 330 6088
ApaLI G'TGCA_C
Cuts at: 4475 5631 4475
Size: 1156 5262
ApoI r'AATT_y
Cuts at: 1493 3119 3296 3727 3953 4105 6214 1493
Size: 1626 177 431 226 152 2109 1697
Fragments arranged by size:
2109 1697 1626 431 226 177 152
AseI AT'TA_AT
Cuts at: 160 1032 4972 160
Size: 872 3940 1606
Fragments arranged by size:
3940 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
AvaI C'yCGr_G
Cuts at: 1870 1943 2242 3743 1870
Size: 73 299 1501 4545
Fragments arranged by size:
4545 1501 299 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2291 2324 2291
Size: 33 6385
BamHI G'GATC_C
Cuts at: 3348 3748 4115 3348
Size: 400 367 5651
Fragments arranged by size:
5651 400 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2224 2307 2372 3739 5194
Size: 325 217 627 437 83 65 1367 1455
Cuts at: 5194 618
Size: 1842
Fragments arranged by size:
1842 1455 1367 627 437 325 217 83
65
BanII G_rGCy'C
Cuts at: 1391 2243 2850 3090 3180 3737 1391
Size: 852 607 240 90 557 4072
Fragments arranged by size:
4072 852 607 557 240 90
BbeI G_GCGC'C
Cuts at: 2228 2228
Size: 6418
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2154 2742 2891 3414 3717 928
Size: 971 255 588 149 523 303 3629
Fragments arranged by size:
3629 971 588 523 303 255 149
BbvCI CC'TCA_GC
Cuts at: 2495 2495
Size: 6418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4609 4643 1903
Size: 34 2672 34 3678
Fragments arranged by size:
3678 2672 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4310 5520 4310
Size: 1210 5208
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6418
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3175 5029 136
Size: 107 122 71 631 605 1503 1854 1525
Fragments arranged by size:
1854 1525 1503 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2386 2916 6413 2386
Size: 530 3497 2391
Fragments arranged by size:
3497 2391 530
BlpI GC'TnA_GC
Cuts at: 2237 3835 2237
Size: 1598 4820
Bme1580I G_kGCm'C
Cuts at: 1790 2850 2998 3180 4479 5635 1790
Size: 1060 148 182 1299 1156 2573
Fragments arranged by size:
2573 1299 1156 1060 182 148
BmgBI CAC'GTC
Cuts at: 1318 2323 1318
Size: 1005 5413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2500 4149 5107
Size: 416 53 83 399 24 1083 1649 958
Cuts at: 5107 442
Size: 1753
Fragments arranged by size:
1753 1649 1083 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6418
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2283 2283
Size: 6418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2663 3477 3490 5078 1239
Size: 953 471 814 13 1588 2579
Fragments arranged by size:
2579 1588 953 814 471 13
Bpu10I CC'TnA_GC
Cuts at: 2348 2495 2348
Size: 147 6271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3245 4543 5408 5706 3245
Size: 1298 865 298 3957
Fragments arranged by size:
3957 1298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5081 882
Size: 617 3582 2219
Fragments arranged by size:
3582 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4209 493
Size: 711 3005 2702
Fragments arranged by size:
3005 2702 711
BsaBI GATnn'nnATC
Cuts at: 6412 6412
Size: 6418
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2225 4225 4607 5242
Size: 53 83 186 936 692 2000 382 635
Cuts at: 5242 275
Size: 1451
Fragments arranged by size:
2000 1451 936 692 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2217 4851 5249 5523 5670 6081 2217
Size: 2634 398 274 147 411 2554
Fragments arranged by size:
2634 2554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6193
Fragments arranged by size:
6193 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2615 1186
Size: 1429 4989
BseYI C'CCAG_C
Cuts at: 2525 2636 2650 5621 6117 2525
Size: 111 14 2971 496 2826
Fragments arranged by size:
2971 2826 496 111 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2594 2645 3050 1163
Size: 573 858 51 405 4531
Fragments arranged by size:
4531 858 573 405 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3868 4629 5657 1540
Size: 275 186 1867 761 1028 2301
Fragments arranged by size:
2301 1867 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2649 3090 4479 4564 5635 2649
Size: 441 1389 85 1071 3432
Fragments arranged by size:
3432 1389 1071 441 85
BsmI GAATG_Cn'
Cuts at: 3929 4022 3929
Size: 93 6325
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2243 2649 2850 2998 3090 3180 3737
Size: 399 453 406 201 148 92 90 557
Cuts at: 3737 4479 4564 5635 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 557 453 406 399 201
148 92 90 85
BspEI T'CCGG_A
Cuts at: 2217 6081 2217
Size: 3864 2554
BspHI T'CATG_A
Cuts at: 3367 3706 4307 6037 3367
Size: 339 601 1730 3748
Fragments arranged by size:
3748 1730 601 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2616 3773 844
Size: 1772 1157 3489
Fragments arranged by size:
3489 1772 1157
BsrBI CCG'CTC
Cuts at: 1394 1823 4305 1394
Size: 429 2482 3507
Fragments arranged by size:
3507 2482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4913 5087 65
Size: 1183 3665 174 1396
Fragments arranged by size:
3665 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3339 3378 5062 5249 1700
Size: 110 1529 39 1684 187 2869
Fragments arranged by size:
2869 1684 1529 187 110 39
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6418
BssSI C'ACGA_G
Cuts at: 2360 4478 5490 2360
Size: 2118 1012 3288
Fragments arranged by size:
3288 2118 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2186 3590 794
Size: 1392 1404 3622
Fragments arranged by size:
3622 1404 1392
BstBI TT'CG_AA
Cuts at: 5222 5222
Size: 6418
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6418
BstXI CCAn_nnnn'nTGG
Cuts at: 2405 2405
Size: 6418
BstYI r'GATC_y
Cuts at: 1972 2386 2705 2916 3348 3748 4115 4500 4517
Size: 414 319 211 432 400 367 385 17
Cuts at: 4517 5969 6055 6067 6413 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 432 414 400 385 367 346
319 211 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2037 2487 2037
Size: 450 5968
BtgI C'CryG_G
Cuts at: 513 1065 2004 2139 2303 513
Size: 552 939 135 164 4628
Fragments arranged by size:
4628 939 552 164 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5679
Fragments arranged by size:
5679 683 56
BtsI GCAGTG_nn'
Cuts at: 2386 3038 3934 4728 4748 2386
Size: 652 896 794 20 4056
Fragments arranged by size:
4056 896 794 652 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2404 2439 3550 3585 529
Size: 35 1840 35 1111 35 3362
Fragments arranged by size:
3362 1840 1111 35 35 35
DraI TTT'AAA
Cuts at: 3722 3722
Size: 6418
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3608 5425 809
Size: 507 2292 1817 1802
Fragments arranged by size:
2292 1817 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2018 2082 2133
Size: 54 1425 50 275 18 188 64 51
Cuts at: 2133 3865 4754 6383 8
Size: 1732 889 1629 43
Fragments arranged by size:
1732 1629 1425 889 275 188 64 54
51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3865 1537
Size: 275 2053 4090
Fragments arranged by size:
4090 2053 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2721 3180 4348 1353
Size: 1368 459 1168 3423
Fragments arranged by size:
3423 1368 1168 459
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5005 5379 5525 387
Size: 1064 138 3416 374 146 1280
Fragments arranged by size:
3416 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3088 3088
Size: 6418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2663 3128 3204 3477 3490 4481
Size: 393 560 471 465 76 273 13 991
Cuts at: 4481 5078 5865 6133 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 991 787 597 560 471 465 393
273 268 76 13
EcoO109I rG'GnC_Cy
Cuts at: 2499 2605 2847 3177 2499
Size: 106 242 330 5740
Fragments arranged by size:
5740 330 242 106
EcoRI G'AATT_C
Cuts at: 3727 4105 3727
Size: 378 6040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2759 3107 2759
Size: 348 6070
FspI TGC'GCA
Cuts at: 4924 4924
Size: 6418
HaeII r_GCGC'y
Cuts at: 1678 2228 5565 1678
Size: 550 3337 2531
Fragments arranged by size:
3337 2531 550
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2212 2230 2244 2262 2723
Size: 32 135 32 640 18 14 18 461
Cuts at: 2723 2755 3626 3658 5059 5091 5133 5165 1373
Size: 32 871 32 1401 32 42 32 2626
Fragments arranged by size:
2626 1401 871 640 461 135 42 32
32 32 32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2989 3762 4008 669
Size: 988 281 1051 773 246 3079
Fragments arranged by size:
3079 1051 988 773 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6418
HpaI GTT'AAC
Cuts at: 4008 4008
Size: 6418
KasI G'GCGC_C
Cuts at: 2224 2224
Size: 6418
KpnI G_GTAC'C
Cuts at: 2311 3743 2311
Size: 1432 4986
MfeI C'AATT_G
Cuts at: 4017 4017
Size: 6418
MluI A'CGCG_T
Cuts at: 6294 6294
Size: 6418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2179 2583 5532 5716 1305
Size: 874 404 2949 184 2007
Fragments arranged by size:
2949 2007 874 404 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 10
Size: 54 1425 343 303 4293
Fragments arranged by size:
4293 1425 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2802 3366 3472 4376 4735 4894 518
Size: 648 1636 564 106 904 359 159 2042
Fragments arranged by size:
2042 1636 904 648 564 359 159 106
MspA1I CmG'CkG
Cuts at: 1606 2170 3834 4511 5659 5904 1606
Size: 564 1664 677 1148 245 2120
Fragments arranged by size:
2120 1664 1148 677 564 245
NarI GG'CG_CC
Cuts at: 2225 2225
Size: 6418
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4927
Fragments arranged by size:
4927 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6418
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6418
NotI GC'GGCC_GC
Cuts at: 3865 3865
Size: 6418
NspI r_CATG'y
Cuts at: 1132 1229 3402 3445 3776 5321 1132
Size: 97 2173 43 331 1545 2229
Fragments arranged by size:
2229 2173 1545 331 97 43
PasI CC'CwG_GG
Cuts at: 1348 2328 1348
Size: 980 5438
PciI A'CATG_T
Cuts at: 3398 5317 3398
Size: 1919 4499
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2801 1108
Size: 1693 4725
PmeI GTTT'AAAC
Cuts at: 3722 3722
Size: 6418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4487 4519 6000 6032 4487
Size: 32 1481 32 4873
Fragments arranged by size:
4873 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2499 2605 2499
Size: 106 6312
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6418
PsiI TTA'TAA
Cuts at: 3988 3988
Size: 6418
PspOMI G'GGCC_C
Cuts at: 2846 3176 2846
Size: 330 6088
PstI C_TGCA'G
Cuts at: 830 2816 3196 3770 830
Size: 1986 380 574 3478
Fragments arranged by size:
3478 1986 574 380
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6418
PvuII CAG'CTG
Cuts at: 2170 2170
Size: 6418
SacI G_AGCT'C
Cuts at: 3090 3090
Size: 6418
SalI G'TCGA_C
Cuts at: 1936 3760 1936
Size: 1824 4594
SbfI CC_TGCA'GG
Cuts at: 3770 3770
Size: 6418
ScaI AGT'ACT
Cuts at: 3566 4666 6378 3566
Size: 1100 1712 3606
Fragments arranged by size:
3606 1712 1100
SfcI C'TryA_G
Cuts at: 826 1045 2119 2255 2812 3192 3202 3766 4901
Size: 219 1074 136 557 380 10 564 1135
Cuts at: 4901 5582 5773 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1074 681 564 557 380 219
191 136 10
SfoI GGC'GCC
Cuts at: 2226 2226
Size: 6418
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6418
SmaI CCC'GGG
Cuts at: 1872 2244 3745 1872
Size: 372 1501 4545
Fragments arranged by size:
4545 1501 372
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3260 4522 5423 5685 820
Size: 197 926 1317 1262 901 262 1553
Fragments arranged by size:
1553 1317 1262 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4209 493
Size: 3716 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6418
SphI G_CATG'C
Cuts at: 1132 3776 1132
Size: 2644 3774
SspI AAT'ATT
Cuts at: 5 52 3242 4342 5
Size: 47 3190 1100 2081
Fragments arranged by size:
3190 2081 1100 47
StuI AGG'CCT
Cuts at: 1517 2679 1517
Size: 1162 5256
StyI C'CwwG_G
Cuts at: 513 1065 2004 3006 3857 513
Size: 552 939 1002 851 3074
Fragments arranged by size:
3074 1002 939 851 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2539 2664 4445 4462 4615 4800 5245
Size: 1255 959 125 1781 17 153 185 445
Cuts at: 5245 325
Size: 1498
Fragments arranged by size:
1781 1498 1255 959 445 185 153 125
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3264 3485 3564
Size: 274 80 33 51 1443 1287 221 79
Cuts at: 3564 4664 6376 96
Size: 1100 1712 138
Fragments arranged by size:
1712 1443 1287 1100 274 221 138 80
79 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2033 2065 2437 2469 6378 6410 2033
Size: 32 372 32 3909 32 2041
Fragments arranged by size:
3909 2041 372 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6418
XbaI T'CTAG_A
Cuts at: 3754 3754
Size: 6418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2402 2405 2948 2402
Size: 3 543 5872
Fragments arranged by size:
5872 543 3
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6418
XmaI C'CCGG_G
Cuts at: 1870 2242 3743 1870
Size: 372 1501 4545
Fragments arranged by size:
4545 1501 372
XmnI GAAnn'nnTTC
Cuts at: 3410 3551 3665 4547 3410
Size: 141 114 882 5281
Fragments arranged by size:
5281 882 141 114
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4226 276
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BfrBI BsiWI BsmBI BstZ17I
DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI