Restriction map for
pFN21AB7874
(Circular) (Six-base) MAPSORT of: pFN21AB7874.seq Check: 5375 from: 1 to: 6616
pFN21AB7874 6616 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4426 278
Size: 53 83 186 936 2890 2468
Fragments arranged by size:
2890 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3959 1937
Size: 2022 4594
Acc65I G'GTAC_C
Cuts at: 2342 2420 3937 2342
Size: 78 1517 5021
Fragments arranged by size:
5021 1517 78
AclI AA'CG_TT
Cuts at: 1563 4743 5116 1563
Size: 3180 373 3063
Fragments arranged by size:
3180 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2916 4679 6063 6331 1632
Size: 1284 1763 1384 268 1917
Fragments arranged by size:
1917 1763 1384 1284 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6419
AflIII A'CryG_T
Cuts at: 1203 2077 2272 5515 6492 1203
Size: 874 195 3243 977 1327
Fragments arranged by size:
3243 1327 977 874 195
AgeI A'CCGG_T
Cuts at: 5447 5447
Size: 6616
AhdI GACnn_n'nnGTC
Cuts at: 5345 5345
Size: 6616
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3666 3705 1184
Size: 2482 39 4095
Fragments arranged by size:
4095 2482 39
AlwNI CAG_nnn'CTG
Cuts at: 2606 3176 4359 5931 2606
Size: 570 1183 1572 3291
Fragments arranged by size:
3291 1572 1183 570
ApaLI G'TGCA_C
Cuts at: 3038 4673 5829 3038
Size: 1635 1156 3825
Fragments arranged by size:
3825 1635 1156
ApoI r'AATT_y
Cuts at: 1493 3925 4151 4303 6412 1493
Size: 2432 226 152 2109 1697
Fragments arranged by size:
2432 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5170 160
Size: 872 4138 1606
Fragments arranged by size:
4138 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6616
AvaI C'yCGr_G
Cuts at: 1870 1943 3941 1870
Size: 73 1998 4545
Fragments arranged by size:
4545 1998 73
BamHI G'GATC_C
Cuts at: 3946 4313 3946
Size: 367 6249
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2342 2420 3744 3815 3937
Size: 325 217 627 555 78 1324 71 122
Cuts at: 3937 5392 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1324 627 555 325 217 122
78 71
BanII G_rGCy'C
Cuts at: 1391 3336 3935 1391
Size: 1945 599 4072
Fragments arranged by size:
4072 1945 599
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3278 928
Size: 971 1379 4266
Fragments arranged by size:
4266 1379 971
BbvCI CC'TCA_GC
Cuts at: 3631 3666 3631
Size: 35 6581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4807 4841 1903
Size: 34 2870 34 3678
Fragments arranged by size:
3678 2870 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3132 4508 5718 3132
Size: 1376 1210 4030
Fragments arranged by size:
4030 1376 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6616
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5227 136
Size: 107 122 71 631 605 3555 1525
Fragments arranged by size:
3555 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2938 3285 6611 2938
Size: 347 3326 2943
Fragments arranged by size:
3326 2943 347
BlpI GC'TnA_GC
Cuts at: 4033 4033
Size: 6616
Bme1580I G_kGCm'C
Cuts at: 1790 3042 3747 4677 5833 1790
Size: 1252 705 930 1156 2573
Fragments arranged by size:
2573 1252 1156 930 705
BmgBI CAC'GTC
Cuts at: 1318 2863 1318
Size: 1545 5071
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2523 4347 5305
Size: 416 53 83 399 24 1106 1824 958
Cuts at: 5305 442
Size: 1753
Fragments arranged by size:
1824 1753 1106 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2076 5276 1239
Size: 837 3200 2579
Fragments arranged by size:
3200 2579 837
Bpu10I CC'TnA_GC
Cuts at: 3631 3666 3631
Size: 35 6581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2747 3522 3687 4741 5606 5904 2747
Size: 775 165 1054 865 298 3459
Fragments arranged by size:
3459 1054 865 775 298 165
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5279 882
Size: 617 3780 2219
Fragments arranged by size:
3780 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3414 4407 493
Size: 711 2210 993 2702
Fragments arranged by size:
2702 2210 993 711
BsaBI GATnn'nnATC
Cuts at: 6610 6610
Size: 6616
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4423 4805 5440 275
Size: 53 83 186 936 2890 382 635 1451
Fragments arranged by size:
2890 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5049 5447 5721 5868 6279 5049
Size: 398 274 147 411 5386
Fragments arranged by size:
5386 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2351 2381 1178
Size: 30 165 30 948 30 5413
Fragments arranged by size:
5413 948 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2073 3356 3359 3619 1186
Size: 887 1283 3 260 4183
Fragments arranged by size:
4183 1283 887 260 3
BseYI C'CCAG_C
Cuts at: 5819 6315 5819
Size: 496 6120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2492 3061 1163
Size: 573 756 569 4718
Fragments arranged by size:
4718 756 573 569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3007 4066 4827 5855 1540
Size: 275 186 1006 1059 761 1028 2301
Fragments arranged by size:
2301 1059 1028 1006 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3042 4677 4762 5833 3042
Size: 1635 85 1071 3825
Fragments arranged by size:
3825 1635 1071 85
BsmI GAATG_Cn'
Cuts at: 3716 4127 4220 3716
Size: 411 93 6112
Fragments arranged by size:
6112 411 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3042 3336 3747 3935 4677 4762 5833
Size: 399 1252 294 411 188 742 85 1071
Cuts at: 5833 1391
Size: 2174
Fragments arranged by size:
2174 1252 1071 742 411 399 294 188
85
BspEI T'CCGG_A
Cuts at: 6279 6279
Size: 6616
BspHI T'CATG_A
Cuts at: 4505 6235 4505
Size: 1730 4886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2492 2591 3971 844
Size: 1648 99 1380 3489
Fragments arranged by size:
3489 1648 1380 99
BsrBI CCG'CTC
Cuts at: 1394 1823 4503 1394
Size: 429 2680 3507
Fragments arranged by size:
3507 2680 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5111 5285 65
Size: 1183 3863 174 1396
Fragments arranged by size:
3863 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5260 5447 1700
Size: 110 3450 187 2869
Fragments arranged by size:
3450 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3167 3213 96
Size: 3071 46 3499
Fragments arranged by size:
3499 3071 46
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6616
BssSI C'ACGA_G
Cuts at: 4676 5688 4676
Size: 1012 5604
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6616
BstBI TT'CG_AA
Cuts at: 2994 5420 2994
Size: 2426 4190
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6616
BstXI CCAn_nnnn'nTGG
Cuts at: 3574 3574
Size: 6616
BstYI r'GATC_y
Cuts at: 1972 2938 3285 3946 4313 4698 4715 6167 6253
Size: 966 347 661 367 385 17 1452 86
Cuts at: 6253 6265 6611 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 966 661 385 367 347 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2109 513
Size: 552 1044 5020
Fragments arranged by size:
5020 1044 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5877
Fragments arranged by size:
5877 683 56
BtsI GCAGTG_nn'
Cuts at: 2451 4132 4926 4946 2451
Size: 1681 794 20 4121
Fragments arranged by size:
4121 1681 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6616
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6581
DraI TTT'AAA
Cuts at: 3920 3920
Size: 6616
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5623 809
Size: 507 4307 1802
Fragments arranged by size:
4307 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2227 3297 4063
Size: 54 1425 50 275 18 397 1070 766
Cuts at: 4063 4952 6581 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1070 889 766 397 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4063 1537
Size: 275 2251 4090
Fragments arranged by size:
4090 2251 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4546 1353
Size: 3193 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5203 5577 5723 387
Size: 1064 138 3614 374 146 1280
Fragments arranged by size:
3614 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2916 4679 5276 6063 6331 1239
Size: 393 444 840 1763 597 787 268 1524
Fragments arranged by size:
1763 1524 840 787 597 444 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2058 2058
Size: 6616
EcoO109I rG'GnC_Cy
Cuts at: 2976 3402 2976
Size: 426 6190
EcoRI G'AATT_C
Cuts at: 3925 4303 3925
Size: 378 6238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3007 3410 3007
Size: 403 6213
FspI TGC'GCA
Cuts at: 5122 5122
Size: 6616
HaeII r_GCGC'y
Cuts at: 1678 5763 1678
Size: 4085 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5257 5289 5331 5363 1373
Size: 32 135 32 3685 32 42 32 2626
Fragments arranged by size:
3685 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3488 3529 3960 4206 669
Size: 988 281 1550 41 431 246 3079
Fragments arranged by size:
3079 1550 988 431 281 246 41
HindIII A'AGCT_T
Cuts at: 748 2149 748
Size: 1401 5215
HpaI GTT'AAC
Cuts at: 4206 4206
Size: 6616
KpnI G_GTAC'C
Cuts at: 2346 2424 3941 2346
Size: 78 1517 5021
Fragments arranged by size:
5021 1517 78
MfeI C'AATT_G
Cuts at: 2526 3693 4215 2526
Size: 1167 522 4927
Fragments arranged by size:
4927 1167 522
MluI A'CGCG_T
Cuts at: 6492 6492
Size: 6616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 3888 3910 5730 5914 1305
Size: 918 1665 22 1820 184 2007
Fragments arranged by size:
2007 1820 1665 918 184 22
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2229 3299 10
Size: 54 1425 343 397 1070 3327
Fragments arranged by size:
3327 1425 1070 397 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2713 2893 3572 4574 4933 5092 518
Size: 648 1547 180 679 1002 359 159 2042
Fragments arranged by size:
2042 1547 1002 679 648 359 180 159
MspA1I CmG'CkG
Cuts at: 1606 2410 4032 4709 5857 6102 1606
Size: 804 1622 677 1148 245 2120
Fragments arranged by size:
2120 1622 1148 804 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6064
NdeI CA'TA_TG
Cuts at: 387 3160 387
Size: 2773 3843
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6616
NotI GC'GGCC_GC
Cuts at: 4063 4063
Size: 6616
NspI r_CATG'y
Cuts at: 1132 1229 2081 2276 2666 2816 3527 3550 3974
Size: 97 852 195 390 150 711 23 424
Cuts at: 3974 5519 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 852 711 424 390 195 150
97 23
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6616
PciI A'CATG_T
Cuts at: 2077 2272 5515 2077
Size: 195 3243 3178
Fragments arranged by size:
3243 3178 195
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3092 1108
Size: 1984 4632
PfoI T'CCnGG_A
Cuts at: 2053 2053
Size: 6616
PmeI GTTT'AAAC
Cuts at: 3920 3920
Size: 6616
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4685 4717 6198 6230 4685
Size: 32 1481 32 5071
Fragments arranged by size:
5071 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3402 3402
Size: 6616
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6616
PsiI TTA'TAA
Cuts at: 3123 4186 3123
Size: 1063 5553
PstI C_TGCA'G
Cuts at: 830 3968 830
Size: 3138 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6616
PvuII CAG'CTG
Cuts at: 2410 2410
Size: 6616
SalI G'TCGA_C
Cuts at: 1936 3958 1936
Size: 2022 4594
SanDI GG'GwC_CC
Cuts at: 3402 3402
Size: 6616
SbfI CC_TGCA'GG
Cuts at: 3968 3968
Size: 6616
ScaI AGT'ACT
Cuts at: 4864 6576 4864
Size: 1712 4904
SfcI C'TryA_G
Cuts at: 826 1045 2942 3964 5099 5780 5971 826
Size: 219 1897 1022 1135 681 191 1471
Fragments arranged by size:
1897 1471 1135 1022 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6616
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6616
SmaI CCC'GGG
Cuts at: 1872 3943 1872
Size: 2071 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 3537 3702 4720 5621 5883
Size: 197 926 819 775 165 1018 901 262
Cuts at: 5883 820
Size: 1553
Fragments arranged by size:
1553 1018 926 901 819 775 262 197
165
SnaBI TAC'GTA
Cuts at: 493 3414 4407 493
Size: 2921 993 2702
Fragments arranged by size:
2921 2702 993
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6616
SphI G_CATG'C
Cuts at: 1132 2816 3974 1132
Size: 1684 1158 3774
Fragments arranged by size:
3774 1684 1158
SspI AAT'ATT
Cuts at: 5 52 3203 4540 5
Size: 47 3151 1337 2081
Fragments arranged by size:
3151 2081 1337 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6616
StyI C'CwwG_G
Cuts at: 513 1065 4055 513
Size: 552 2990 3074
Fragments arranged by size:
3074 2990 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2997 3168 3833 4643 4660 4813 4998
Size: 1255 1417 171 665 810 17 153 185
Cuts at: 4998 5443 325
Size: 445 1498
Fragments arranged by size:
1498 1417 1255 810 665 445 185 171
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2326 2912 3167
Size: 274 80 33 51 1443 349 586 255
Cuts at: 3167 3213 4862 6574 96
Size: 46 1649 1712 138
Fragments arranged by size:
1712 1649 1443 586 349 274 255 138
80 51 46 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2351 2383 3224 3256 6576 6608 2351
Size: 32 841 32 3320 32 2359
Fragments arranged by size:
3320 2359 841 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3483 1316
Size: 2167 4449
XbaI T'CTAG_A
Cuts at: 2308 3878 3952 2308
Size: 1570 74 4972
Fragments arranged by size:
4972 1570 74
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2548 2548
Size: 6616
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6616
XmaI C'CCGG_G
Cuts at: 1870 3941 1870
Size: 2071 4545
XmnI GAAnn'nnTTC
Cuts at: 3390 4745 3390
Size: 1355 5261
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4424 276
Size: 53 83 186 936 2890 2468
Fragments arranged by size:
2890 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI