Restriction map for
pFN21AB7869
(Circular) (Six-base) MAPSORT of: pFN21AB7869.seq Check: 6434 from: 1 to: 6388
pFN21AB7869 6388 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3731 1937
Size: 1794 4594
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 4515 4888 1563
Size: 2952 373 3063
Fragments arranged by size:
3063 2952 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2802 3172 3256 4451 5835 6103 1632
Size: 1170 370 84 1195 1384 268 1917
Fragments arranged by size:
1917 1384 1195 1170 370 268 84
AfeI AGC'GCT
Cuts at: 2439 3269 3446 2439
Size: 830 177 5381
Fragments arranged by size:
5381 830 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 5287 6264 1203
Size: 4084 977 1327
Fragments arranged by size:
4084 1327 977
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 2486 3147 1166
Size: 1320 661 4407
Fragments arranged by size:
4407 1320 661
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2886 1184
Size: 1702 4686
AlwNI CAG_nnn'CTG
Cuts at: 2314 4131 5703 2314
Size: 1817 1572 2999
Fragments arranged by size:
2999 1817 1572
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 2008 2634 2773 3280 3555 3639 3697 3923
Size: 515 626 139 507 275 84 58 226
Cuts at: 3923 4075 6184 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 626 515 507 275 226 152
139 84 58
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 2288 2354 3713 1870
Size: 73 345 66 1359 4545
Fragments arranged by size:
4545 1359 345 73 66
BamHI G'GATC_C
Cuts at: 3718 4085 3718
Size: 367 6021
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2089 3709 5164 618
Size: 325 217 627 302 1620 1455 1842
Fragments arranged by size:
1842 1620 1455 627 325 302 217
BanII G_rGCy'C
Cuts at: 1391 2157 2428 3707 1391
Size: 766 271 1279 4072
Fragments arranged by size:
4072 1279 766 271
BbeI G_GCGC'C
Cuts at: 2093 2093
Size: 6388
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 2847 928
Size: 971 240 708 4469
Fragments arranged by size:
4469 971 708 240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2367 2401 4579 4613 1903
Size: 34 430 34 2178 34 3678
Fragments arranged by size:
3678 2178 430 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4280 5490 4280
Size: 1210 5178
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2659 4999 136
Size: 107 122 71 631 605 987 2340 1525
Fragments arranged by size:
2340 1525 987 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6383 6383
Size: 6388
BlpI GC'TnA_GC
Cuts at: 2249 3805 2249
Size: 1556 4832
Bme1580I G_kGCm'C
Cuts at: 1790 4449 5605 1790
Size: 2659 1156 2573
Fragments arranged by size:
2659 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2979 1318
Size: 1661 4727
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 3573 4119
Size: 416 53 83 399 24 2072 84 546
Cuts at: 4119 5077 442
Size: 958 1753
Fragments arranged by size:
2072 1753 958 546 416 399 84 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2314 2152
Size: 162 6226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2351 5048 1239
Size: 1097 15 2697 2579
Fragments arranged by size:
2697 2579 1097 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4513 5378 5676 4513
Size: 865 298 5225
Fragments arranged by size:
5225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2278 2742 3038 5051 882
Size: 617 779 464 296 2013 2219
Fragments arranged by size:
2219 2013 779 617 464 296
BsaAI yAC'GTr
Cuts at: 493 1204 2986 4179 493
Size: 711 1782 1193 2702
Fragments arranged by size:
2702 1782 1193 711
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2090 4195 4577
Size: 53 83 186 936 510 47 2105 382
Cuts at: 4577 5212 275
Size: 635 1451
Fragments arranged by size:
2105 1451 936 635 510 382 186 83
53 47
BsaWI w'CCGG_w
Cuts at: 2236 2744 4821 5219 5493 5640 6051 2236
Size: 508 2077 398 274 147 411 2573
Fragments arranged by size:
2573 2077 508 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6163
Fragments arranged by size:
6163 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2372 2417 2519 1186
Size: 1186 45 102 5055
Fragments arranged by size:
5055 1186 102 45
BseYI C'CCAG_C
Cuts at: 2524 3665 5591 6087 2524
Size: 1141 1926 496 2825
Fragments arranged by size:
2825 1926 1141 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 1163
Size: 573 447 5368
Fragments arranged by size:
5368 573 447
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2385 2576 4449 4534 5605 2385
Size: 191 1873 85 1071 3168
Fragments arranged by size:
3168 1873 1071 191 85
BsmI GAATG_Cn'
Cuts at: 3347 3428 3899 3992 3347
Size: 81 471 93 5743
Fragments arranged by size:
5743 471 93 81
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2385 2428 2576 3707 4449 4534
Size: 399 367 228 43 148 1131 742 85
Cuts at: 4534 5605 1391
Size: 1071 2174
Fragments arranged by size:
2174 1131 1071 742 399 367 228 148
85 43
BspEI T'CCGG_A
Cuts at: 2744 6051 2744
Size: 3307 3081
BspHI T'CATG_A
Cuts at: 4277 6007 4277
Size: 1730 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3743 844
Size: 2899 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2397 4275 1394
Size: 429 574 1878 3507
Fragments arranged by size:
3507 1878 574 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4883 5057 65
Size: 1183 3635 174 1396
Fragments arranged by size:
3635 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2253 5032 5219 1700
Size: 110 443 2779 187 2869
Fragments arranged by size:
2869 2779 443 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6388
BstBI TT'CG_AA
Cuts at: 5192 5192
Size: 6388
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6388
BstXI CCAn_nnnn'nTGG
Cuts at: 3176 3176
Size: 6388
BstYI r'GATC_y
Cuts at: 1972 2310 3718 4085 4470 4487 5939 6025 6037
Size: 338 1408 367 385 17 1452 86 12
Cuts at: 6037 6383 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1408 385 367 346 338 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2166 2415 513
Size: 552 1101 249 4486
Fragments arranged by size:
4486 1101 552 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5649
Fragments arranged by size:
5649 683 56
BtsI GCAGTG_nn'
Cuts at: 2044 2383 3904 4698 4718 2044
Size: 339 1521 794 20 3714
Fragments arranged by size:
3714 1521 794 339 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 3331 3460 3692 3331
Size: 129 232 6027
Fragments arranged by size:
6027 232 129
DraIII CAC_nnn'GTG
Cuts at: 2974 2974
Size: 6388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2412 3835 4724
Size: 54 1425 50 275 18 582 1423 889
Cuts at: 4724 6353 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1423 889 582 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2235 3092 3425 4318 1353
Size: 882 857 333 893 3423
Fragments arranged by size:
3423 893 882 857 333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2571 4975 5349 5495 387
Size: 1064 138 982 2404 374 146 1280
Fragments arranged by size:
2404 1280 1064 982 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2351 2802 3172 3256 4451 5048
Size: 393 704 15 451 370 84 1195 597
Cuts at: 5048 5835 6103 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1195 787 704 597 451 393 370
268 84 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2354 2354
Size: 6388
EcoO109I rG'GnC_Cy
Cuts at: 2054 2054
Size: 6388
EcoRI G'AATT_C
Cuts at: 2008 3280 3555 3639 3697 4075 2008
Size: 1272 275 84 58 378 4321
Fragments arranged by size:
4321 1272 378 275 84 58
FspI TGC'GCA
Cuts at: 2556 4894 2556
Size: 2338 4050
HaeII r_GCGC'y
Cuts at: 1678 2093 2249 2441 2518 2692 3271 3448 5535
Size: 415 156 192 77 174 579 177 2087
Cuts at: 5535 1678
Size: 2531
Fragments arranged by size:
2531 2087 579 415 192 177 174 156
77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 3489 3521 5029
Size: 32 135 32 863 32 1022 32 1508
Cuts at: 5029 5061 5103 5135 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1508 1022 863 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3732 3978 669
Size: 988 281 1794 246 3079
Fragments arranged by size:
3079 1794 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3184 748
Size: 2436 3952
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KasI G'GCGC_C
Cuts at: 2089 2089
Size: 6388
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 3987 3987
Size: 6388
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2907 3390 3652 5502 5686 1305
Size: 1602 483 262 1850 184 2007
Fragments arranged by size:
2007 1850 1602 483 262 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4566
Fragments arranged by size:
4566 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 3147 4346 4705 4864 518
Size: 648 1320 661 1199 359 159 2042
Fragments arranged by size:
2042 1320 1199 661 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2168 2212 3804 4481 5629 5874 1606
Size: 562 44 1592 677 1148 245 2120
Fragments arranged by size:
2120 1592 1148 677 562 245 44
NaeI GCC'GGC
Cuts at: 2255 2255
Size: 6388
NarI GG'CG_CC
Cuts at: 2090 2090
Size: 6388
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5836
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6388
NgoMIV G'CCGG_C
Cuts at: 2253 2253
Size: 6388
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 3746 5291 1132
Size: 97 2517 1545 2229
Fragments arranged by size:
2517 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2058 2458 1348
Size: 710 400 5278
Fragments arranged by size:
5278 710 400
PciI A'CATG_T
Cuts at: 5287 5287
Size: 6388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2227 2464 1108
Size: 1119 237 5032
Fragments arranged by size:
5032 1119 237
PfoI T'CCnGG_A
Cuts at: 2313 2567 2313
Size: 254 6134
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PmlI CAC'GTG
Cuts at: 2986 2986
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 5970 6002 4457
Size: 32 1481 32 4843
Fragments arranged by size:
4843 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2054 2054
Size: 6388
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3078 3958 3078
Size: 880 5508
PstI C_TGCA'G
Cuts at: 830 2433 2546 3740 830
Size: 1603 113 1194 3478
Fragments arranged by size:
3478 1603 1194 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 2212 2212
Size: 6388
SacII CC_GC'GG
Cuts at: 2169 2169
Size: 6388
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SanDI GG'GwC_CC
Cuts at: 2054 2054
Size: 6388
SapI GCTCTTCn'nnn_
Cuts at: 3425 3425
Size: 6388
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 2429 2542 3736 4871 5552 5743 826
Size: 219 1384 113 1194 1135 681 191 1471
Fragments arranged by size:
1471 1384 1194 1135 681 219 191 113
SfoI GGC'GCC
Cuts at: 2091 2091
Size: 6388
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 3715 1872
Size: 1843 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4492 5393 5655 820
Size: 197 926 2549 901 262 1553
Fragments arranged by size:
2549 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3746 1132
Size: 2614 3774
SspI AAT'ATT
Cuts at: 5 52 4312 5
Size: 47 4260 2081
Fragments arranged by size:
4260 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6388
StyI C'CwwG_G
Cuts at: 513 1065 2079 2301 3827 513
Size: 552 1014 222 1526 3074
Fragments arranged by size:
3074 1526 1014 552 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2050 2362 4415 4432 4585 4770 5215
Size: 1255 470 312 2053 17 153 185 445
Cuts at: 5215 325
Size: 1498
Fragments arranged by size:
2053 1498 1255 470 445 312 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3614 4634 6346
Size: 274 80 33 51 1443 1637 1020 1712
Cuts at: 6346 96
Size: 138
Fragments arranged by size:
1712 1637 1443 1020 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2046 2078 3609 3641 6348 6380 2046
Size: 32 1531 32 2707 32 2054
Fragments arranged by size:
2707 2054 1531 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6388
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2228 2228
Size: 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 3713 1870
Size: 1843 4545
XmnI GAAnn'nnTTC
Cuts at: 3101 3185 3434 3518 4517 3101
Size: 84 249 84 999 4972
Fragments arranged by size:
4972 999 249 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacI SexAI SfiI SrfI SwaI