Restriction map for
pFN21AB7868
(Circular) (Six-base) MAPSORT of: pFN21AB7868.seq Check: 627 from: 1 to: 6607
pFN21AB7868 6607 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4417 278
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2898 3950 1937
Size: 961 1052 4594
Fragments arranged by size:
4594 1052 961
Acc65I G'GTAC_C
Cuts at: 3928 3928
Size: 6607
AclI AA'CG_TT
Cuts at: 1563 3906 4734 5107 1563
Size: 2343 828 373 3063
Fragments arranged by size:
3063 2343 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2614 4670 6054 6322 1632
Size: 982 2056 1384 268 1917
Fragments arranged by size:
2056 1917 1384 982 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6410
AflIII A'CryG_T
Cuts at: 1203 2101 3841 5506 6483 1203
Size: 898 1740 1665 977 1327
Fragments arranged by size:
1740 1665 1327 977 898
AgeI A'CCGG_T
Cuts at: 5438 5438
Size: 6607
AhdI GACnn_n'nnGTC
Cuts at: 5336 5336
Size: 6607
AleI CACnn'nnGTG
Cuts at: 1166 3383 1166
Size: 2217 4390
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6607
AlwNI CAG_nnn'CTG
Cuts at: 2090 2158 2687 2738 3283 4350 5922 2090
Size: 68 529 51 545 1067 1572 2775
Fragments arranged by size:
2775 1572 1067 545 529 68 51
ApaLI G'TGCA_C
Cuts at: 2431 4664 5820 2431
Size: 2233 1156 3218
Fragments arranged by size:
3218 2233 1156
ApoI r'AATT_y
Cuts at: 1493 3916 4142 4294 6403 1493
Size: 2423 226 152 2109 1697
Fragments arranged by size:
2423 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5161 160
Size: 872 4129 1606
Fragments arranged by size:
4129 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 3932 1870
Size: 73 1989 4545
Fragments arranged by size:
4545 1989 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2090 2123 2090
Size: 33 6574
BamHI G'GATC_C
Cuts at: 3937 4304 3937
Size: 367 6240
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 2521 3702 3928 5383
Size: 325 217 627 525 209 1181 226 1455
Cuts at: 5383 618
Size: 1842
Fragments arranged by size:
1842 1455 1181 627 525 325 226 217
209
BanII G_rGCy'C
Cuts at: 1391 2215 2567 2847 3105 3926 1391
Size: 824 352 280 258 821 4072
Fragments arranged by size:
4072 824 821 352 280 258
BbeI G_GCGC'C
Cuts at: 2316 2316
Size: 6607
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3280 3294 3608 928
Size: 971 1381 14 314 3927
Fragments arranged by size:
3927 1381 971 314 14
BbvCI CC'TCA_GC
Cuts at: 2354 2461 3071 2354
Size: 107 610 5890
Fragments arranged by size:
5890 610 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3797 3831 4798 4832 1903
Size: 34 1860 34 967 34 3678
Fragments arranged by size:
3678 1860 967 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4499 5709 4499
Size: 1210 5397
BclI T'GATC_A
Cuts at: 1548 2254 1548
Size: 706 5901
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5218 136
Size: 107 122 71 631 605 3546 1525
Fragments arranged by size:
3546 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2956 2983 3456 6602 2956
Size: 27 473 3146 2961
Fragments arranged by size:
3146 2961 473 27
BlpI GC'TnA_GC
Cuts at: 4024 4024
Size: 6607
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2435 2543 3380 4668 5824 1790
Size: 341 304 108 837 1288 1156 2573
Fragments arranged by size:
2573 1288 1156 837 341 304 108
BmgBI CAC'GTC
Cuts at: 1318 3381 3842 1318
Size: 2063 461 4083
Fragments arranged by size:
4083 2063 461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2022 2289 2902
Size: 416 53 83 399 24 605 267 613
Cuts at: 2902 4338 5296 442
Size: 1436 958 1753
Fragments arranged by size:
1753 1436 958 613 605 416 399 267
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3795 3795
Size: 6607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2569 2801 2981 3019 3447 5267 1239
Size: 1330 232 180 38 428 1820 2579
Fragments arranged by size:
2579 1820 1330 428 232 180 38
Bpu10I CC'TnA_GC
Cuts at: 2354 2461 3071 2354
Size: 107 610 5890
Fragments arranged by size:
5890 610 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3602 4732 5597 5895 3602
Size: 1130 865 298 4314
Fragments arranged by size:
4314 1130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2845 3620 5270 882
Size: 617 1346 775 1650 2219
Fragments arranged by size:
2219 1650 1346 775 617
BsaAI yAC'GTr
Cuts at: 493 1204 2902 3514 4398 493
Size: 711 1698 612 884 2702
Fragments arranged by size:
2702 1698 884 711 612
BsaBI GATnn'nnATC
Cuts at: 6601 6601
Size: 6607
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2313 4414 4796 5431
Size: 53 83 186 936 780 2101 382 635
Cuts at: 5431 275
Size: 1451
Fragments arranged by size:
2101 1451 936 780 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2847 5040 5438 5712 5859 6270 2847
Size: 2193 398 274 147 411 3184
Fragments arranged by size:
3184 2193 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3382 3412 3891 3921 1178
Size: 30 165 30 1979 30 479 30 3864
Fragments arranged by size:
3864 1979 479 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2007 2227 2727 2978 2987 2990 3134 3231
Size: 821 220 500 251 9 3 144 97
Cuts at: 3231 3912 1186
Size: 681 3881
Fragments arranged by size:
3881 821 681 500 251 220 144 97
9 3
BseYI C'CCAG_C
Cuts at: 2516 2612 2731 2840 2923 5810 6306 2516
Size: 96 119 109 83 2887 496 2817
Fragments arranged by size:
2887 2817 496 119 109 96 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2213 1163
Size: 573 477 5557
Fragments arranged by size:
5557 573 477
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3248 3539 4057 4818 5846 1540
Size: 275 186 1247 291 518 761 1028 2301
Fragments arranged by size:
2301 1247 1028 761 518 291 275 186
BsiHKAI G_wGCw'C
Cuts at: 2406 2435 3597 4668 4753 5824 2406
Size: 29 1162 1071 85 1071 3189
Fragments arranged by size:
3189 1162 1071 1071 85 29
BsmI GAATG_Cn'
Cuts at: 4118 4211 4118
Size: 93 6514
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2215 2406 2435 2543 2567 2847
Size: 399 341 84 191 29 108 24 280
Cuts at: 2847 3105 3380 3597 3926 4668 4753 5824 1391
Size: 258 275 217 329 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 341 329 280 275
258 217 191 108 85 84 29 24
BspEI T'CCGG_A
Cuts at: 2847 6270 2847
Size: 3423 3184
BspHI T'CATG_A
Cuts at: 4496 6226 4496
Size: 1730 4877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3115 3176 3962 844
Size: 2271 61 786 3489
Fragments arranged by size:
3489 2271 786 61
BsrBI CCG'CTC
Cuts at: 1394 1823 3250 4494 1394
Size: 429 1427 1244 3507
Fragments arranged by size:
3507 1427 1244 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5102 5276 65
Size: 1183 3854 174 1396
Fragments arranged by size:
3854 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3507 3699 5251 5438 1700
Size: 110 1697 192 1552 187 2869
Fragments arranged by size:
2869 1697 1552 192 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6607
BssHII G'CGCG_C
Cuts at: 1925 2570 1925
Size: 645 5962
BssSI C'ACGA_G
Cuts at: 4667 5679 4667
Size: 1012 5595
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2158 2687 794
Size: 1364 529 4714
Fragments arranged by size:
4714 1364 529
BstBI TT'CG_AA
Cuts at: 5411 5411
Size: 6607
BstEII G'GTnAC_C
Cuts at: 1184 2119 1184
Size: 935 5672
BstXI CCAn_nnnn'nTGG
Cuts at: 2302 2302
Size: 6607
BstYI r'GATC_y
Cuts at: 1972 2956 2983 3279 3456 3937 4304 4689 4706
Size: 984 27 296 177 481 367 385 17
Cuts at: 4706 6158 6244 6256 6602 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 984 481 385 367 346 296
177 86 27 17 12
BstZ17I GTA'TAC
Cuts at: 2899 2899
Size: 6607
Bsu36I CC'TnA_GG
Cuts at: 2115 3060 2115
Size: 945 5662
BtgI C'CryG_G
Cuts at: 513 1065 2004 2559 3369 3747 513
Size: 552 939 555 810 378 3373
Fragments arranged by size:
3373 939 810 555 552 378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2190 2814 487
Size: 683 56 964 624 4280
Fragments arranged by size:
4280 964 683 624 56
BtsI GCAGTG_nn'
Cuts at: 2100 2697 2704 2755 2832 3369 3445 4123 4917
Size: 597 7 51 77 537 76 678 794
Cuts at: 4917 4937 2100
Size: 20 3770
Fragments arranged by size:
3770 794 678 597 537 77 76 51
20 7
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6607
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6572
DraI TTT'AAA
Cuts at: 3911 3911
Size: 6607
DraIII CAC_nnn'GTG
Cuts at: 2422 3871 2422
Size: 1449 5158
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5614 809
Size: 507 4298 1802
Fragments arranged by size:
4298 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2347 3328 3536
Size: 54 1425 50 275 18 517 981 208
Cuts at: 3536 3819 4054 4943 6572 8
Size: 283 235 889 1629 43
Fragments arranged by size:
1629 1425 981 889 517 283 275 235
208 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3536 4054 1537
Size: 275 1724 518 4090
Fragments arranged by size:
4090 1724 518 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2964 3223 4537 1353
Size: 1611 259 1314 3423
Fragments arranged by size:
3423 1611 1314 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2055 5194 5568 5714 387
Size: 1064 138 466 3139 374 146 1280
Fragments arranged by size:
3139 1280 1064 466 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2569 2614 2801 2981 3019 3447 4670
Size: 393 937 45 187 180 38 428 1223
Cuts at: 4670 5267 6054 6322 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1223 937 787 597 428 393 268
187 180 45 38
EcoNI CCTnn'n_nnAGG
Cuts at: 3183 3183
Size: 6607
EcoO109I rG'GnC_Cy
Cuts at: 2260 3056 3176 3421 2260
Size: 796 120 245 5446
Fragments arranged by size:
5446 796 245 120
EcoRI G'AATT_C
Cuts at: 3916 4294 3916
Size: 378 6229
FspI TGC'GCA
Cuts at: 5113 5113
Size: 6607
HaeII r_GCGC'y
Cuts at: 1678 2316 2930 3700 5754 1678
Size: 638 614 770 2054 2531
Fragments arranged by size:
2531 2054 770 638 614
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2591 2623 2642 2674 5248
Size: 32 135 32 1019 32 19 32 2574
Cuts at: 5248 5280 5322 5354 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2574 1019 135 42 32 32 32
32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 2380 2994 3130 3951 4197 669
Size: 988 281 442 614 136 821 246 3079
Fragments arranged by size:
3079 988 821 614 442 281 246 136
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6607
HpaI GTT'AAC
Cuts at: 4197 4197
Size: 6607
KasI G'GCGC_C
Cuts at: 2312 2312
Size: 6607
KpnI G_GTAC'C
Cuts at: 3932 3932
Size: 6607
MfeI C'AATT_G
Cuts at: 4206 4206
Size: 6607
MluI A'CGCG_T
Cuts at: 6483 6483
Size: 6607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5721 5905 1305
Size: 4416 184 2007
Fragments arranged by size:
4416 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3330 10
Size: 54 1425 343 1498 3287
Fragments arranged by size:
3287 1498 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3383 4565 4924 5083 518
Size: 648 2217 1182 359 159 2042
Fragments arranged by size:
2217 2042 1182 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2053 2090 3801 4023 4700 5848 6093 1606
Size: 447 37 1711 222 677 1148 245 2120
Fragments arranged by size:
2120 1711 1148 677 447 245 222 37
NaeI GCC'GGC
Cuts at: 3701 3701
Size: 6607
NarI GG'CG_CC
Cuts at: 2313 2313
Size: 6607
NcoI C'CATG_G
Cuts at: 513 1065 2004 2559 513
Size: 552 939 555 4561
Fragments arranged by size:
4561 939 555 552
NdeI CA'TA_TG
Cuts at: 387 3163 387
Size: 2776 3831
NgoMIV G'CCGG_C
Cuts at: 3699 3699
Size: 6607
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6607
NotI GC'GGCC_GC
Cuts at: 4054 4054
Size: 6607
NspI r_CATG'y
Cuts at: 1132 1229 2105 3268 3502 3523 3965 5510 1132
Size: 97 876 1163 234 21 442 1545 2229
Fragments arranged by size:
2229 1545 1163 876 442 234 97 21
PasI CC'CwG_GG
Cuts at: 1348 2637 1348
Size: 1289 5318
PciI A'CATG_T
Cuts at: 2101 5506 2101
Size: 3405 3202
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3283 3871 1108
Size: 2175 588 3844
Fragments arranged by size:
3844 2175 588
PfoI T'CCnGG_A
Cuts at: 2528 3781 2528
Size: 1253 5354
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PmlI CAC'GTG
Cuts at: 3514 3514
Size: 6607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4676 4708 6189 6221 4676
Size: 32 1481 32 5062
Fragments arranged by size:
5062 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2260 3176 3421 2260
Size: 916 245 5446
Fragments arranged by size:
5446 916 245
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6607
PsiI TTA'TAA
Cuts at: 4177 4177
Size: 6607
PstI C_TGCA'G
Cuts at: 830 2095 2163 2491 2692 3959 830
Size: 1265 68 328 201 1267 3478
Fragments arranged by size:
3478 1267 1265 328 201 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
PvuII CAG'CTG
Cuts at: 2090 2090
Size: 6607
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SanDI GG'GwC_CC
Cuts at: 3421 3421
Size: 6607
SbfI CC_TGCA'GG
Cuts at: 3959 3959
Size: 6607
ScaI AGT'ACT
Cuts at: 4855 6567 4855
Size: 1712 4895
SfcI C'TryA_G
Cuts at: 826 1045 2091 2159 2487 2688 3955 5090 5771
Size: 219 1046 68 328 201 1267 1135 681
Cuts at: 5771 5962 826
Size: 191 1471
Fragments arranged by size:
1471 1267 1135 1046 681 328 219 201
191 68
SfoI GGC'GCC
Cuts at: 2314 2314
Size: 6607
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6607
SmaI CCC'GGG
Cuts at: 1872 3934 1872
Size: 2062 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3617 4711 5612 5874 820
Size: 197 926 1674 1094 901 262 1553
Fragments arranged by size:
1674 1553 1094 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6607
SphI G_CATG'C
Cuts at: 1132 3502 3965 1132
Size: 2370 463 3774
Fragments arranged by size:
3774 2370 463
SspI AAT'ATT
Cuts at: 5 52 4531 5
Size: 47 4479 2081
Fragments arranged by size:
4479 2081 47
StuI AGG'CCT
Cuts at: 1517 3482 1517
Size: 1965 4642
StyI C'CwwG_G
Cuts at: 513 1065 2004 2559 3258 4046 513
Size: 552 939 555 699 788 3074
Fragments arranged by size:
3074 939 788 699 555 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 3871 4634 4651 4804 4989 5434
Size: 1255 1806 485 763 17 153 185 445
Cuts at: 5434 325
Size: 1498
Fragments arranged by size:
1806 1498 1255 763 485 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4853 6565 96
Size: 274 80 33 51 1443 2876 1712 138
Fragments arranged by size:
2876 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3382 3414 3424 3456 3846 3878 6567 6599 3382
Size: 32 10 32 390 32 2689 32 3390
Fragments arranged by size:
3390 2689 390 32 32 32 32 10
Tth111I GACn'n_nGTC
Cuts at: 1316 2375 1316
Size: 1059 5548
XbaI T'CTAG_A
Cuts at: 3943 3943
Size: 6607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2889 3043 2131
Size: 758 154 5695
Fragments arranged by size:
5695 758 154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6607
XmaI C'CCGG_G
Cuts at: 1870 3932 1870
Size: 2062 4545
XmnI GAAnn'nnTTC
Cuts at: 3482 4736 3482
Size: 1254 5353
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4415 276
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI
BsmBI EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI