Restriction map for
pFN21AB7867
(Circular) (Six-base) MAPSORT of: pFN21AB7867.seq Check: 8435 from: 1 to: 6385
pFN21AB7867 6385 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2239 2564 3323 2239
Size: 325 759 5301
Fragments arranged by size:
5301 759 325
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3639 4195 278
Size: 53 83 186 936 2103 556 2468
Fragments arranged by size:
2468 2103 936 556 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3728 1937
Size: 1791 4594
Acc65I G'GTAC_C
Cuts at: 3706 3706
Size: 6385
AclI AA'CG_TT
Cuts at: 1563 4512 4885 1563
Size: 2949 373 3063
Fragments arranged by size:
3063 2949 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4448 5832 6100 1632
Size: 2816 1384 268 1917
Fragments arranged by size:
2816 1917 1384 268
AfeI AGC'GCT
Cuts at: 3239 3239
Size: 6385
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6188
AflIII A'CryG_T
Cuts at: 1203 2124 5284 6261 1203
Size: 921 3160 977 1327
Fragments arranged by size:
3160 1327 977 921
AgeI A'CCGG_T
Cuts at: 5216 5216
Size: 6385
AhdI GACnn_n'nnGTC
Cuts at: 5114 5114
Size: 6385
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6385
AlwNI CAG_nnn'CTG
Cuts at: 3082 3338 4128 5700 3082
Size: 256 790 1572 3767
Fragments arranged by size:
3767 1572 790 256
ApaI G_GGCC'C
Cuts at: 2690 2690
Size: 6385
ApaLI G'TGCA_C
Cuts at: 4442 5598 4442
Size: 1156 5229
ApoI r'AATT_y
Cuts at: 1493 3694 3920 4072 6181 1493
Size: 2201 226 152 2109 1697
Fragments arranged by size:
2201 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4939 160
Size: 872 3907 1606
Fragments arranged by size:
3907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
AvaI C'yCGr_G
Cuts at: 1870 1943 3383 3488 3710 1870
Size: 73 1440 105 222 4545
Fragments arranged by size:
4545 1440 222 105 73
BamHI G'GATC_C
Cuts at: 2064 3715 4082 2064
Size: 1651 367 4367
Fragments arranged by size:
4367 1651 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2251 2542 2971 3532 3706
Size: 325 217 627 464 291 429 561 174
Cuts at: 3706 5161 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 561 464 429 325 291
217 174
BanII G_rGCy'C
Cuts at: 1391 2370 2690 3273 3704 1391
Size: 979 320 583 431 4072
Fragments arranged by size:
4072 979 583 431 320
BbeI G_GCGC'C
Cuts at: 2546 2975 2546
Size: 429 5956
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2026 928
Size: 971 127 5287
Fragments arranged by size:
5287 971 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2776 2810 3092 3126 4576 4610 1903
Size: 34 839 34 282 34 1450 34 3678
Fragments arranged by size:
3678 1450 839 282 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4277 5487 4277
Size: 1210 5175
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6385
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2654 3324 4996
Size: 107 122 71 631 605 982 670 1672
Cuts at: 4996 136
Size: 1525
Fragments arranged by size:
1672 1525 982 670 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6380 6380
Size: 6385
BlpI GC'TnA_GC
Cuts at: 3339 3593 3802 3339
Size: 254 209 5922
Fragments arranged by size:
5922 254 209
Bme1580I G_kGCm'C
Cuts at: 1790 2690 4446 5602 1790
Size: 900 1756 1156 2573
Fragments arranged by size:
2573 1756 1156 900
BmgBI CAC'GTC
Cuts at: 1318 2032 1318
Size: 714 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4116 5074 442
Size: 416 53 83 399 24 2699 958 1753
Fragments arranged by size:
2699 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6385
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2890 2890
Size: 6385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2649 3461 3543 5045 1239
Size: 1037 373 812 82 1502 2579
Fragments arranged by size:
2579 1502 1037 812 373 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4510 5375 5673 4510
Size: 865 298 5222
Fragments arranged by size:
5222 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3245 5048 882
Size: 617 1746 1803 2219
Fragments arranged by size:
2219 1803 1746 617
BsaAI yAC'GTr
Cuts at: 493 1204 2127 3229 3676 4176 493
Size: 711 923 1102 447 500 2702
Fragments arranged by size:
2702 1102 923 711 500 447
BsaBI GATnn'nnATC
Cuts at: 6379 6379
Size: 6385
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2543 2972 3500 3636
Size: 53 83 186 936 1010 429 528 136
Cuts at: 3636 4192 4574 5209 275
Size: 556 382 635 1451
Fragments arranged by size:
1451 1010 936 635 556 528 429 382
186 136 83 53
BsaWI w'CCGG_w
Cuts at: 2134 4818 5216 5490 5637 6048 2134
Size: 2684 398 274 147 411 2471
Fragments arranged by size:
2684 2471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2984 3014 1178
Size: 30 165 30 1581 30 4549
Fragments arranged by size:
4549 1581 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2290 2969 2998 3005 3008 1186
Size: 967 137 679 29 7 3 4563
Fragments arranged by size:
4563 967 679 137 29 7 3
BseYI C'CCAG_C
Cuts at: 2546 2879 3331 3467 5588 6084 2546
Size: 333 452 136 2121 496 2847
Fragments arranged by size:
2847 2121 496 452 333 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 1163
Size: 573 531 5281
Fragments arranged by size:
5281 573 531
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3351 3835 4596 5624 1540
Size: 275 186 1350 484 761 1028 2301
Fragments arranged by size:
2301 1350 1028 761 484 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 2370 2665 4446 4531 5602 2031
Size: 339 295 1781 85 1071 2814
Fragments arranged by size:
2814 1781 1071 339 295 85
BsmI GAATG_Cn'
Cuts at: 3896 3989 3896
Size: 93 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2370 2665 2690 3273 3704 4446
Size: 399 241 339 295 25 583 431 742
Cuts at: 4446 4531 5602 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 583 431 399 339 295
241 85 25
BspEI T'CCGG_A
Cuts at: 6048 6048
Size: 6385
BspHI T'CATG_A
Cuts at: 4274 6004 4274
Size: 1730 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2239 2523 2564 3114 3323 3363 3589 3740
Size: 1395 284 41 550 209 40 226 151
Cuts at: 3740 844
Size: 3489
Fragments arranged by size:
3489 1395 550 284 226 209 151 41
40
BsrBI CCG'CTC
Cuts at: 1394 1823 2413 2445 4272 1394
Size: 429 590 32 1827 3507
Fragments arranged by size:
3507 1827 590 429 32
BsrDI GCAATG_nn'
Cuts at: 65 1248 4880 5054 65
Size: 1183 3632 174 1396
Fragments arranged by size:
3632 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2285 2648 2707 3032 3496 5029 5216
Size: 110 475 363 59 325 464 1533 187
Cuts at: 5216 1700
Size: 2869
Fragments arranged by size:
2869 1533 475 464 363 325 187 110
59
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6385
BssHII G'CGCG_C
Cuts at: 1925 2334 3293 1925
Size: 409 959 5017
Fragments arranged by size:
5017 959 409
BssSI C'ACGA_G
Cuts at: 4445 5457 4445
Size: 1012 5373
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3082 3240 794
Size: 2288 158 3939
Fragments arranged by size:
3939 2288 158
BstBI TT'CG_AA
Cuts at: 5189 5189
Size: 6385
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6385
BstXI CCAn_nnnn'nTGG
Cuts at: 3319 3319
Size: 6385
BstYI r'GATC_y
Cuts at: 1972 2064 3715 4082 4467 4484 5936 6022 6034
Size: 92 1651 367 385 17 1452 86 12
Cuts at: 6034 6380 1972
Size: 346 1977
Fragments arranged by size:
1977 1651 1452 385 367 346 92 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3256 513
Size: 552 939 1252 3642
Fragments arranged by size:
3642 1252 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5646
Fragments arranged by size:
5646 683 56
BtsI GCAGTG_nn'
Cuts at: 3901 4695 4715 3901
Size: 794 20 5571
Fragments arranged by size:
5571 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6350
DraI TTT'AAA
Cuts at: 2935 3689 2935
Size: 754 5631
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3499 5392 809
Size: 507 2183 1893 1802
Fragments arranged by size:
2183 1893 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2530 2646 3348
Size: 54 1425 50 275 18 700 116 702
Cuts at: 3348 3832 4721 6350 8
Size: 484 889 1629 43
Fragments arranged by size:
1629 1425 889 702 700 484 275 116
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3348 3832 1537
Size: 275 1536 484 4090
Fragments arranged by size:
4090 1536 484 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2210 4315 1353
Size: 857 2105 3423
Fragments arranged by size:
3423 2105 857
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3563 4972 5346 5492 387
Size: 1064 138 1974 1409 374 146 1280
Fragments arranged by size:
1974 1409 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2368 2368
Size: 6385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2649 3461 3543 4448 5045 5832
Size: 393 644 373 812 82 905 597 787
Cuts at: 5832 6100 1239
Size: 268 1524
Fragments arranged by size:
1524 905 812 787 644 597 393 373
268 82
EcoO109I rG'GnC_Cy
Cuts at: 2686 2687 3118 2686
Size: 1 431 5953
Fragments arranged by size:
5953 431 1
EcoRI G'AATT_C
Cuts at: 3694 4072 3694
Size: 378 6007
EcoRV GAT'ATC
Cuts at: 2210 2210
Size: 6385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3082 3628 3082
Size: 546 5839
FspI TGC'GCA
Cuts at: 3233 4891 3233
Size: 1658 4727
HaeII r_GCGC'y
Cuts at: 1678 2546 2911 2975 3241 3346 5532 1678
Size: 868 365 64 266 105 2186 2531
Fragments arranged by size:
2531 2186 868 365 266 105 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2194 2226 2984 3016 3020
Size: 32 135 32 622 32 758 32 4
Cuts at: 3020 3052 5026 5058 5100 5132 1373
Size: 32 1974 32 42 32 2626
Fragments arranged by size:
2626 1974 758 622 135 42 32 32
32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3729 3975 669
Size: 988 281 1791 246 3079
Fragments arranged by size:
3079 1791 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6385
HpaI GTT'AAC
Cuts at: 3975 3975
Size: 6385
KasI G'GCGC_C
Cuts at: 2542 2971 2542
Size: 429 5956
KpnI G_GTAC'C
Cuts at: 3710 3710
Size: 6385
MfeI C'AATT_G
Cuts at: 3984 3984
Size: 6385
MluI A'CGCG_T
Cuts at: 6261 6261
Size: 6385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5499 5683 1305
Size: 4194 184 2007
Fragments arranged by size:
4194 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4563
Fragments arranged by size:
4563 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4343 4702 4861 518
Size: 648 3177 359 159 2042
Fragments arranged by size:
3177 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2200 3338 3530 3801 4478 5626 5871
Size: 412 182 1138 192 271 677 1148 245
Cuts at: 5871 1606
Size: 2120
Fragments arranged by size:
2120 1148 1138 677 412 271 245 192
182
NaeI GCC'GGC
Cuts at: 2709 3034 2709
Size: 325 6060
NarI GG'CG_CC
Cuts at: 2543 2972 2543
Size: 429 5956
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4894
Fragments arranged by size:
4894 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6385
NgoMIV G'CCGG_C
Cuts at: 2707 3032 2707
Size: 325 6060
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6385
NotI GC'GGCC_GC
Cuts at: 3832 3832
Size: 6385
NspI r_CATG'y
Cuts at: 1132 1229 2827 3743 5288 1132
Size: 97 1598 916 1545 2229
Fragments arranged by size:
2229 1598 1545 916 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6385
PciI A'CATG_T
Cuts at: 5284 5284
Size: 6385
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2021 1108
Size: 913 5472
PfoI T'CCnGG_A
Cuts at: 2148 2292 2148
Size: 144 6241
PmeI GTTT'AAAC
Cuts at: 3689 3689
Size: 6385
PmlI CAC'GTG
Cuts at: 2127 3229 2127
Size: 1102 5283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4454 4486 5967 5999 4454
Size: 32 1481 32 4840
Fragments arranged by size:
4840 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3118 3118
Size: 6385
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6385
PsiI TTA'TAA
Cuts at: 3955 3955
Size: 6385
PspOMI G'GGCC_C
Cuts at: 2686 2686
Size: 6385
PstI C_TGCA'G
Cuts at: 830 2520 3087 3586 3737 830
Size: 1690 567 499 151 3478
Fragments arranged by size:
3478 1690 567 499 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6385
PvuII CAG'CTG
Cuts at: 2018 2200 2018
Size: 182 6203
SacI G_AGCT'C
Cuts at: 2370 2370
Size: 6385
SalI G'TCGA_C
Cuts at: 1936 3727 1936
Size: 1791 4594
SanDI GG'GwC_CC
Cuts at: 3118 3118
Size: 6385
SbfI CC_TGCA'GG
Cuts at: 3737 3737
Size: 6385
ScaI AGT'ACT
Cuts at: 2453 4633 6345 2453
Size: 2180 1712 2493
Fragments arranged by size:
2493 2180 1712
SfcI C'TryA_G
Cuts at: 826 1045 2516 3083 3582 3733 4868 5549 5740
Size: 219 1471 567 499 151 1135 681 191
Cuts at: 5740 826
Size: 1471
Fragments arranged by size:
1471 1471 1135 681 567 499 219 191
151
SfoI GGC'GCC
Cuts at: 2544 2973 2544
Size: 429 5956
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
SgrAI Cr'CCGG_yG
Cuts at: 1700 3496 1700
Size: 1796 4589
SmaI CCC'GGG
Cuts at: 1872 3712 1872
Size: 1840 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4489 5390 5652 820
Size: 197 926 2546 901 262 1553
Fragments arranged by size:
2546 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4176 493
Size: 3683 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6385
SphI G_CATG'C
Cuts at: 1132 2827 3743 1132
Size: 1695 916 3774
Fragments arranged by size:
3774 1695 916
SspI AAT'ATT
Cuts at: 5 52 4309 5
Size: 47 4257 2081
Fragments arranged by size:
4257 2081 47
StuI AGG'CCT
Cuts at: 1517 3065 1517
Size: 1548 4837
StyI C'CwwG_G
Cuts at: 513 1065 2004 3057 3824 513
Size: 552 939 1053 767 3074
Fragments arranged by size:
3074 1053 939 767 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3550 4412 4429 4582 4767 5212 325
Size: 1255 1970 862 17 153 185 445 1498
Fragments arranged by size:
1970 1498 1255 862 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2451 4631 6343
Size: 274 80 33 51 1443 474 2180 1712
Cuts at: 6343 96
Size: 138
Fragments arranged by size:
2180 1712 1443 474 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2775 2807 6345 6377 2775
Size: 32 3538 32 2783
Fragments arranged by size:
3538 2783 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6385
XbaI T'CTAG_A
Cuts at: 3721 3721
Size: 6385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2239 2326 2623 2239
Size: 87 297 6001
Fragments arranged by size:
6001 297 87
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6385
XmaI C'CCGG_G
Cuts at: 1870 3710 1870
Size: 1840 4545
XmnI GAAnn'nnTTC
Cuts at: 4514 4514
Size: 6385
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3637 4193 276
Size: 53 83 186 936 2103 556 2468
Fragments arranged by size:
2468 2103 936 556 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SacII SapI SexAI
SfiI SrfI SwaI