Restriction map for
pFN21AB7865
(Circular) (Six-base) MAPSORT of: pFN21AB7865.seq Check: 3360 from: 1 to: 6376
pFN21AB7865 6376 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2892 3719 1937
Size: 955 827 4594
Fragments arranged by size:
4594 955 827
Acc65I G'GTAC_C
Cuts at: 3697 3697
Size: 6376
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4439 5823 6091 1632
Size: 2807 1384 268 1917
Fragments arranged by size:
2807 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 3537 3639 5275 6252 1203
Size: 2334 102 1636 977 1327
Fragments arranged by size:
2334 1636 1327 977 102
AgeI A'CCGG_T
Cuts at: 2706 5207 2706
Size: 2501 3875
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AlwNI CAG_nnn'CTG
Cuts at: 2611 3436 4119 5691 2611
Size: 825 683 1572 3296
Fragments arranged by size:
3296 1572 825 683
ApaI G_GGCC'C
Cuts at: 2724 2724
Size: 6376
ApaLI G'TGCA_C
Cuts at: 4433 5589 4433
Size: 1156 5220
ApoI r'AATT_y
Cuts at: 1493 3685 3911 4063 6172 1493
Size: 2192 226 152 2109 1697
Fragments arranged by size:
2192 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4930 160
Size: 872 3898 1606
Fragments arranged by size:
3898 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 3701 1870
Size: 73 1758 4545
Fragments arranged by size:
4545 1758 73
BamHI G'GATC_C
Cuts at: 3706 4073 3706
Size: 367 6009
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2474 3697 5152 618
Size: 325 217 627 687 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 687 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2060 2172 2243 2682 2724 2867 3476 3695
Size: 669 112 71 439 42 143 609 219
Cuts at: 3695 1391
Size: 4072
Fragments arranged by size:
4072 669 609 439 219 143 112 71
42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2179 2492 3636 928
Size: 971 280 313 1144 3668
Fragments arranged by size:
3668 1144 971 313 280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4567 4601 1903
Size: 34 2630 34 3678
Fragments arranged by size:
3678 2630 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4268 5478 4268
Size: 1210 5166
BclI T'GATC_A
Cuts at: 1548 2376 1548
Size: 828 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4987 136
Size: 107 122 71 631 605 3315 1525
Fragments arranged by size:
3315 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6371 6371
Size: 6376
BlpI GC'TnA_GC
Cuts at: 2054 3793 2054
Size: 1739 4637
Bme1580I G_kGCm'C
Cuts at: 1790 2477 2724 4437 5593 1790
Size: 687 247 1713 1156 2573
Fragments arranged by size:
2573 1713 1156 687 247
BmgBI CAC'GTC
Cuts at: 1318 2022 1318
Size: 704 5672
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3233 3559 4107
Size: 416 53 83 399 24 1816 326 548
Cuts at: 4107 5065 442
Size: 958 1753
Fragments arranged by size:
1816 1753 958 548 416 399 326 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6376
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2558 2558
Size: 6376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2012 2153 3342 5036 1239
Size: 773 141 1189 1694 2579
Fragments arranged by size:
2579 1694 1189 773 141
Bpu10I CC'TnA_GC
Cuts at: 2042 2042
Size: 6376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 4501 5366 5664 2264
Size: 2237 865 298 2976
Fragments arranged by size:
2976 2237 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5039 882
Size: 617 3540 2219
Fragments arranged by size:
3540 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4167 493
Size: 711 2963 2702
Fragments arranged by size:
2963 2702 711
BsaBI GATnn'nnATC
Cuts at: 6370 6370
Size: 6376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2952 4183 4565 5200
Size: 53 83 186 936 1419 1231 382 635
Cuts at: 5200 275
Size: 1451
Fragments arranged by size:
1451 1419 1231 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2337 2706 4809 5207 5481 5628 6039 2337
Size: 369 2103 398 274 147 411 2674
Fragments arranged by size:
2674 2103 411 398 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 3254 3284 1178
Size: 30 165 30 822 30 999 30 4270
Fragments arranged by size:
4270 999 822 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2321 2936 3016 3021 3082 3179 3240
Size: 925 210 615 80 5 61 97 61
Cuts at: 3240 1186
Size: 4322
Fragments arranged by size:
4322 925 615 210 97 80 61 61
5
BseYI C'CCAG_C
Cuts at: 2457 2730 2771 3020 3160 3285 3429 3492 5579
Size: 273 41 249 140 125 144 63 2087
Cuts at: 5579 6075 2457
Size: 496 2758
Fragments arranged by size:
2758 2087 496 273 249 144 140 125
63 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3422 1163
Size: 573 1686 4117
Fragments arranged by size:
4117 1686 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2758 3826 4587 5615 1540
Size: 275 186 757 1068 761 1028 2301
Fragments arranged by size:
2301 1068 1028 761 757 275 186
BsiHKAI G_wGCw'C
Cuts at: 2060 4437 4522 5593 2060
Size: 2377 85 1071 2843
Fragments arranged by size:
2843 2377 1071 85
BsmI GAATG_Cn'
Cuts at: 2810 2927 3887 3980 2810
Size: 117 960 93 5206
Fragments arranged by size:
5206 960 117 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2129 2129
Size: 6376
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2172 2243 2477 2682 2724 2867
Size: 399 270 112 71 234 205 42 143
Cuts at: 2867 3476 3695 4437 4522 5593 1391
Size: 609 219 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 609 399 270 234 219
205 143 112 85 71 42
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 3306 4265 5995 3306
Size: 959 1730 3687
Fragments arranged by size:
3687 1730 959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3731 844
Size: 2887 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2504 4263 1394
Size: 429 681 1759 3507
Fragments arranged by size:
3507 1759 681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3603 4871 5045 65
Size: 1183 2355 1268 174 1396
Fragments arranged by size:
2355 1396 1268 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2182 2432 2706 3392 5020 5207 1700
Size: 110 372 250 274 686 1628 187 2869
Fragments arranged by size:
2869 1628 686 372 274 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 2508 96
Size: 2412 3964
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6376
BstBI TT'CG_AA
Cuts at: 5180 5180
Size: 6376
BstEII G'GTnAC_C
Cuts at: 1184 2065 3127 1184
Size: 881 1062 4433
Fragments arranged by size:
4433 1062 881
BstXI CCAn_nnnn'nTGG
Cuts at: 2573 2651 2573
Size: 78 6298
BstYI r'GATC_y
Cuts at: 1972 2538 3706 4073 4458 4475 5927 6013 6025
Size: 566 1168 367 385 17 1452 86 12
Cuts at: 6025 6371 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1168 566 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2644 2854 2946 2998 3109 3123
Size: 552 939 640 210 92 52 111 14
Cuts at: 3123 513
Size: 3766
Fragments arranged by size:
3766 939 640 552 210 111 92 52
14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5637
Fragments arranged by size:
5637 683 56
BtsI GCAGTG_nn'
Cuts at: 3892 4686 4706 3892
Size: 794 20 5562
Fragments arranged by size:
5562 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6341
DraI TTT'AAA
Cuts at: 3680 3680
Size: 6376
DraIII CAC_nnn'GTG
Cuts at: 3368 3368
Size: 6376
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3136 5383 809
Size: 507 1820 2247 1802
Fragments arranged by size:
2247 1820 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2943 3390
Size: 54 1425 50 275 18 376 737 447
Cuts at: 3390 3823 4712 6341 8
Size: 433 889 1629 43
Fragments arranged by size:
1629 1425 889 737 447 433 376 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3823 1537
Size: 275 2011 4090
Fragments arranged by size:
4090 2011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2775 4306 1353
Size: 1422 1531 3423
Fragments arranged by size:
3423 1531 1422
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2330 2342 4963 5337 5483 387
Size: 1064 138 741 12 2621 374 146 1280
Fragments arranged by size:
2621 1280 1064 741 374 146 138 12
EcoICRI GAG'CTC
Cuts at: 2058 2058
Size: 6376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 2153 3342 4439 5036 5823 6091
Size: 393 380 141 1189 1097 597 787 268
Cuts at: 6091 1239
Size: 1524
Fragments arranged by size:
1524 1189 1097 787 597 393 380 268
141
EcoO109I rG'GnC_Cy
Cuts at: 2353 2720 2353
Size: 367 6009
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2239 2203
Size: 36 6340
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 2049 2297 3123 5523 1678
Size: 371 248 826 2400 2531
Fragments arranged by size:
2531 2400 826 371 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2657 2689 5017
Size: 32 135 32 653 32 400 32 2328
Cuts at: 5017 5049 5091 5123 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2328 653 400 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2636 3720 3966 669
Size: 988 281 698 1084 246 3079
Fragments arranged by size:
3079 1084 988 698 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6376
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KpnI G_GTAC'C
Cuts at: 3701 3701
Size: 6376
MfeI C'AATT_G
Cuts at: 3333 3975 3333
Size: 642 5734
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2569 2892 2988 5490 5674 1305
Size: 1264 323 96 2502 184 2007
Fragments arranged by size:
2502 2007 1264 323 184 96
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2945 10
Size: 54 1425 343 1113 3441
Fragments arranged by size:
3441 1425 1113 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 3638 4334 4693 4852 518
Size: 648 1314 1158 696 359 159 2042
Fragments arranged by size:
2042 1314 1158 696 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2160 2323 2608 3436 3512 3523 3792 4469
Size: 554 163 285 828 76 11 269 677
Cuts at: 4469 5617 5862 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 828 677 554 285 269 245
163 76 11
NaeI GCC'GGC
Cuts at: 3394 3394
Size: 6376
NcoI C'CATG_G
Cuts at: 513 1065 2004 2644 2946 3109 3123 513
Size: 552 939 640 302 163 14 3766
Fragments arranged by size:
3766 939 640 552 302 163 14
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NgoMIV G'CCGG_C
Cuts at: 3392 3392
Size: 6376
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6376
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 2392 3541 3643 3734 5279 1132
Size: 97 1163 1149 102 91 1545 2229
Fragments arranged by size:
2229 1545 1163 1149 102 97 91
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6376
PciI A'CATG_T
Cuts at: 3537 3639 5275 3537
Size: 102 1636 4638
Fragments arranged by size:
4638 1636 102
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2860 1108
Size: 1752 4624
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4445 4477 5958 5990 4445
Size: 32 1481 32 4831
Fragments arranged by size:
4831 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2353 2353
Size: 6376
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PspOMI G'GGCC_C
Cuts at: 2720 2720
Size: 6376
PstI C_TGCA'G
Cuts at: 830 2292 3414 3528 3728 830
Size: 1462 1122 114 200 3478
Fragments arranged by size:
3478 1462 1122 200 114
PvuI CG_AT'CG
Cuts at: 2001 2758 2001
Size: 757 5619
PvuII CAG'CTG
Cuts at: 2608 3436 3512 3523 2608
Size: 828 76 11 5461
Fragments arranged by size:
5461 828 76 11
SacI G_AGCT'C
Cuts at: 2060 2060
Size: 6376
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SexAI A'CCwGG_T
Cuts at: 2513 2513
Size: 6376
SfcI C'TryA_G
Cuts at: 826 1045 2288 2956 3058 3410 3524 3724 4859
Size: 219 1243 668 102 352 114 200 1135
Cuts at: 4859 5540 5731 826
Size: 681 191 1471
Fragments arranged by size:
1471 1243 1135 681 668 352 219 200
191 114 102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 3703 1872
Size: 1831 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 4480 5381 5643 820
Size: 197 926 336 2201 901 262 1553
Fragments arranged by size:
2201 1553 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SspI AAT'ATT
Cuts at: 5 52 4300 5
Size: 47 4248 2081
Fragments arranged by size:
4248 2081 47
StuI AGG'CCT
Cuts at: 1517 2090 2750 2792 1517
Size: 573 660 42 5101
Fragments arranged by size:
5101 660 573 42
StyI C'CwwG_G
Cuts at: 513 1065 2004 2070 2091 2214 2357 2644 2946
Size: 552 939 66 21 123 143 287 302
Cuts at: 2946 3109 3123 3815 513
Size: 163 14 692 3074
Fragments arranged by size:
3074 939 692 552 302 287 163 143
123 66 21 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4403 4420 4573 4758 5203 325
Size: 1255 2823 17 153 185 445 1498
Fragments arranged by size:
2823 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2508 3626 4622
Size: 274 80 33 51 1443 531 1118 996
Cuts at: 4622 6334 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1118 996 531 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2259 2291 2332 2364 3254 3286 3354 3386 6336
Size: 32 41 32 890 32 68 32 2950
Cuts at: 6336 6368 2259
Size: 32 2267
Fragments arranged by size:
2950 2267 890 68 41 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6376
XbaI T'CTAG_A
Cuts at: 3712 3712
Size: 6376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2077 2077
Size: 6376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 3701 1870
Size: 1831 4545
XmnI GAAnn'nnTTC
Cuts at: 4505 4505
Size: 6376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI