Restriction map for
pFN21AB7864
(Circular) (Six-base) MAPSORT of: pFN21AB7864.seq Check: 8061 from: 1 to: 6598
pFN21AB7864 6598 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2941 3744 3840 2941
Size: 803 96 5699
Fragments arranged by size:
5699 803 96
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4408 278
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2796 3038 3941 1937
Size: 859 242 903 4594
Fragments arranged by size:
4594 903 859 242
Acc65I G'GTAC_C
Cuts at: 3919 3919
Size: 6598
AclI AA'CG_TT
Cuts at: 1563 4725 5098 1563
Size: 3162 373 3063
Fragments arranged by size:
3162 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4661 6045 6313 1632
Size: 3029 1384 268 1917
Fragments arranged by size:
3029 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6401
AflIII A'CryG_T
Cuts at: 1203 2856 5497 6474 1203
Size: 1653 2641 977 1327
Fragments arranged by size:
2641 1653 1327 977
AgeI A'CCGG_T
Cuts at: 5429 5429
Size: 6598
AhdI GACnn_n'nnGTC
Cuts at: 5327 5327
Size: 6598
AleI CACnn'nnGTG
Cuts at: 1166 2786 1166
Size: 1620 4978
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3806 1184
Size: 2622 3976
AlwNI CAG_nnn'CTG
Cuts at: 2092 2644 2764 3122 3406 3644 3736 4341 5913
Size: 552 120 358 284 238 92 605 1572
Cuts at: 5913 2092
Size: 2777
Fragments arranged by size:
2777 1572 605 552 358 284 238 120
92
ApaI G_GGCC'C
Cuts at: 2109 2627 2871 3131 2109
Size: 518 244 260 5576
Fragments arranged by size:
5576 518 260 244
ApaLI G'TGCA_C
Cuts at: 4655 5811 4655
Size: 1156 5442
ApoI r'AATT_y
Cuts at: 1493 3907 4133 4285 6394 1493
Size: 2414 226 152 2109 1697
Fragments arranged by size:
2414 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5152 160
Size: 872 4120 1606
Fragments arranged by size:
4120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 2205 2887 3524 3923 1870
Size: 73 262 682 637 399 4545
Fragments arranged by size:
4545 682 637 399 262 73
BamHI G'GATC_C
Cuts at: 3928 4295 3928
Size: 367 6231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3293 3919 5374 618
Size: 325 217 627 1506 626 1455 1842
Fragments arranged by size:
1842 1506 1455 627 626 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 2109 2213 2571 2627 2771 2871 3078
Size: 649 69 104 358 56 144 100 207
Cuts at: 3078 3131 3500 3806 3917 1391
Size: 53 369 306 111 4072
Fragments arranged by size:
4072 649 369 358 306 207 144 111
104 100 69 56 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2247 2474 3622 928
Size: 971 348 227 1148 3904
Fragments arranged by size:
3904 1148 971 348 227
BbvCI CC'TCA_GC
Cuts at: 2279 2279
Size: 6598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4789 4823 1903
Size: 34 2852 34 3678
Fragments arranged by size:
3678 2852 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4490 5700 4490
Size: 1210 5388
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6598
BfrBI ATG'CAT
Cuts at: 2727 2727
Size: 6598
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2191 3482 5209
Size: 107 122 71 631 605 519 1291 1727
Cuts at: 5209 136
Size: 1525
Fragments arranged by size:
1727 1525 1291 631 605 519 122 107
71
BglII A'GATC_T
Cuts at: 6593 6593
Size: 6598
BlpI GC'TnA_GC
Cuts at: 2066 2178 3486 3755 4015 2066
Size: 112 1308 269 260 4649
Fragments arranged by size:
4649 1308 269 260 112
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2459 2627 2871 3131 4659 5815 1790
Size: 319 350 168 244 260 1528 1156 2573
Fragments arranged by size:
2573 1528 1156 350 319 260 244 168
BmgBI CAC'GTC
Cuts at: 1318 3703 1318
Size: 2385 4213
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2102 2940 3529
Size: 416 53 83 399 24 685 838 589
Cuts at: 3529 4329 5287 442
Size: 800 958 1753
Fragments arranged by size:
1753 958 838 800 685 589 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6598
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3280 3280
Size: 6598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2075 2142 2291 3092 3637 3763 3796
Size: 830 6 67 149 801 545 126 33
Cuts at: 3796 5258 1239
Size: 1462 2579
Fragments arranged by size:
2579 1462 830 801 545 149 126 67
33 6
Bpu10I CC'TnA_GC
Cuts at: 2279 3593 2279
Size: 1314 5284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2992 4723 5588 5886 2992
Size: 1731 865 298 3704
Fragments arranged by size:
3704 1731 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2312 3118 3854 5261 882
Size: 617 813 806 736 1407 2219
Fragments arranged by size:
2219 1407 813 806 736 617
BsaAI yAC'GTr
Cuts at: 493 1204 2859 4389 493
Size: 711 1655 1530 2702
Fragments arranged by size:
2702 1655 1530 711
BsaBI GATnn'nnATC
Cuts at: 6592 6592
Size: 6598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4405 4787 5422 275
Size: 53 83 186 936 2872 382 635 1451
Fragments arranged by size:
2872 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5031 5429 5703 5850 6261 5031
Size: 398 274 147 411 5368
Fragments arranged by size:
5368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2324 2354 2596 2626 1178
Size: 30 165 30 921 30 242 30 5150
Fragments arranged by size:
5150 921 242 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 2699 2720 1186
Size: 1117 396 21 5064
Fragments arranged by size:
5064 1117 396 21
BseYI C'CCAG_C
Cuts at: 2194 2626 2634 2764 3077 3122 3399 3707 5801
Size: 432 8 130 313 45 277 308 2094
Cuts at: 5801 6297 2194
Size: 496 2495
Fragments arranged by size:
2495 2094 496 432 313 308 277 130
45 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3146 3392 3464 1163
Size: 573 1410 246 72 4297
Fragments arranged by size:
4297 1410 573 246 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2164 4048 4809 5837 1540
Size: 275 186 163 1884 761 1028 2301
Fragments arranged by size:
2301 1884 1028 761 275 186 163
BsiHKAI G_wGCw'C
Cuts at: 2040 3806 4659 4744 5815 2040
Size: 1766 853 85 1071 2823
Fragments arranged by size:
2823 1766 1071 853 85
BsmI GAATG_Cn'
Cuts at: 2729 4109 4202 2729
Size: 1380 93 5125
Fragments arranged by size:
5125 1380 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2216 2687 2216
Size: 471 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2109 2213 2459 2571 2627 2771
Size: 399 250 69 104 246 112 56 144
Cuts at: 2771 2871 3078 3131 3500 3806 3917 4659 4744
Size: 100 207 53 369 306 111 742 85
Cuts at: 4744 5815 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 306 250 246
207 144 112 111 104 100 85 69
56 53
BspEI T'CCGG_A
Cuts at: 6261 6261
Size: 6598
BspHI T'CATG_A
Cuts at: 3237 4487 6217 3237
Size: 1250 1730 3618
Fragments arranged by size:
3618 1730 1250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2379 2902 2941 3616 3744 3840 3953 844
Size: 1535 523 39 675 128 96 113 3489
Fragments arranged by size:
3489 1535 675 523 128 113 96 39
BsrBI CCG'CTC
Cuts at: 1394 1823 2906 3430 4485 1394
Size: 429 1083 524 1055 3507
Fragments arranged by size:
3507 1083 1055 524 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3823 5093 5267 65
Size: 1183 2575 1270 174 1396
Fragments arranged by size:
2575 1396 1270 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2814 3343 3676 3722 3742 3750 5242
Size: 110 1004 529 333 46 20 8 1492
Cuts at: 5242 5429 1700
Size: 187 2869
Fragments arranged by size:
2869 1492 1004 529 333 187 110 46
20 8
BsrGI T'GTAC_A
Cuts at: 96 2490 96
Size: 2394 4204
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6598
BssSI C'ACGA_G
Cuts at: 4658 5670 4658
Size: 1012 5586
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3568 794
Size: 2774 3824
BstBI TT'CG_AA
Cuts at: 5402 5402
Size: 6598
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6598
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 3070 3787 3880 2194
Size: 876 717 93 4912
Fragments arranged by size:
4912 876 717 93
BstYI r'GATC_y
Cuts at: 1972 2640 3928 4295 4680 4697 6149 6235 6247
Size: 668 1288 367 385 17 1452 86 12
Cuts at: 6247 6593 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1288 668 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2286 2286
Size: 6598
BtgI C'CryG_G
Cuts at: 513 1065 2004 2310 2339 2850 2956 3063 3480
Size: 552 939 306 29 511 106 107 417
Cuts at: 3480 513
Size: 3631
Fragments arranged by size:
3631 939 552 511 417 306 107 106
29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5859
Fragments arranged by size:
5859 683 56
BtsI GCAGTG_nn'
Cuts at: 2078 3158 4114 4908 4928 2078
Size: 1080 956 794 20 3748
Fragments arranged by size:
3748 1080 956 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6563
DraI TTT'AAA
Cuts at: 3902 3902
Size: 6598
DraIII CAC_nnn'GTG
Cuts at: 2096 2962 3055 3338 2096
Size: 866 93 283 5356
Fragments arranged by size:
5356 866 283 93
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5605 809
Size: 507 4289 1802
Fragments arranged by size:
4289 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2847 3474 3603
Size: 54 1425 50 275 18 1017 627 129
Cuts at: 3603 3820 4045 4934 6563 8
Size: 217 225 889 1629 43
Fragments arranged by size:
1629 1425 1017 889 627 275 225 217
129 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4045 1537
Size: 275 2233 4090
Fragments arranged by size:
4090 2233 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2559 3892 4528 1353
Size: 1206 1333 636 3423
Fragments arranged by size:
3423 1333 1206 636
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2617 2735 5185 5559 5705 387
Size: 1064 138 1028 118 2450 374 146 1280
Fragments arranged by size:
2450 1280 1064 1028 374 146 138 118
EcoICRI GAG'CTC
Cuts at: 2038 3804 2038
Size: 1766 4832
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2075 2142 2291 3092 3637 3763
Size: 393 437 6 67 149 801 545 126
Cuts at: 3763 3796 4661 5258 6045 6313 1239
Size: 33 865 597 787 268 1524
Fragments arranged by size:
1524 865 801 787 597 545 437 393
268 149 126 67 33 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2138 2327 2450 2648 2659 2138
Size: 189 123 198 11 6077
Fragments arranged by size:
6077 198 189 123 11
EcoO109I rG'GnC_Cy
Cuts at: 2105 2106 2129 2335 2623 2868 2891 3127 3128
Size: 1 23 206 288 245 23 236 1
Cuts at: 3128 3272 3715 2105
Size: 144 443 4988
Fragments arranged by size:
4988 443 288 245 236 206 144 23
23 1 1
EcoRI G'AATT_C
Cuts at: 3907 4285 3907
Size: 378 6220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3646 3646
Size: 6598
FspI TGC'GCA
Cuts at: 5104 5104
Size: 6598
HaeII r_GCGC'y
Cuts at: 1678 3600 3762 5745 1678
Size: 1922 162 1983 2531
Fragments arranged by size:
2531 1983 1922 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3164 3196 5239 5271 5313
Size: 32 135 32 1592 32 2043 32 42
Cuts at: 5313 5345 1373
Size: 32 2626
Fragments arranged by size:
2626 2043 1592 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3942 4188 669
Size: 988 281 2004 246 3079
Fragments arranged by size:
3079 2004 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6598
HpaI GTT'AAC
Cuts at: 4188 4188
Size: 6598
KpnI G_GTAC'C
Cuts at: 3923 3923
Size: 6598
MfeI C'AATT_G
Cuts at: 4197 4197
Size: 6598
MluI A'CGCG_T
Cuts at: 6474 6474
Size: 6598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2796 3867 5712 5896 1305
Size: 1491 1071 1845 184 2007
Fragments arranged by size:
2007 1845 1491 1071 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2849 3476 3605 3822 10
Size: 54 1425 343 1017 627 129 217 2786
Fragments arranged by size:
2786 1425 1017 627 343 217 129 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 2786 3878 4556 4915 5074 518
Size: 648 1296 324 1092 678 359 159 2042
Fragments arranged by size:
2042 1296 1092 678 648 359 324 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2395 2865 3015 3122 3406 3490 4014
Size: 420 369 470 150 107 284 84 524
Cuts at: 4014 4691 5839 6084 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 524 470 420 369 284
245 150 107 84
NaeI GCC'GGC
Cuts at: 3678 3724 3744 3678
Size: 46 20 6532
Fragments arranged by size:
6532 46 20
NcoI C'CATG_G
Cuts at: 513 1065 2004 2850 3480 513
Size: 552 939 846 630 3631
Fragments arranged by size:
3631 939 846 630 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6598
NgoMIV G'CCGG_C
Cuts at: 3676 3722 3742 3676
Size: 46 20 6532
Fragments arranged by size:
6532 46 20
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6598
NotI GC'GGCC_GC
Cuts at: 4045 4045
Size: 6598
NsiI A_TGCA'T
Cuts at: 2729 2729
Size: 6598
NspI r_CATG'y
Cuts at: 1132 1229 3956 5501 1132
Size: 97 2727 1545 2229
Fragments arranged by size:
2727 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2876 3268 1348
Size: 1528 392 4678
Fragments arranged by size:
4678 1528 392
PciI A'CATG_T
Cuts at: 5497 5497
Size: 6598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2092 1108
Size: 984 5614
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4667 4699 6180 6212 4667
Size: 32 1481 32 5053
Fragments arranged by size:
5053 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2335 2891 3272 3715 2335
Size: 556 381 443 5218
Fragments arranged by size:
5218 556 443 381
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6598
PsiI TTA'TAA
Cuts at: 4168 4168
Size: 6598
PspOMI G'GGCC_C
Cuts at: 2105 2623 2867 3127 2105
Size: 518 244 260 5576
Fragments arranged by size:
5576 518 260 244
PstI C_TGCA'G
Cuts at: 830 2031 2331 2376 2998 3495 3741 3950 830
Size: 1201 300 45 622 497 246 209 3478
Fragments arranged by size:
3478 1201 622 497 300 246 209 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6598
PvuII CAG'CTG
Cuts at: 2026 3122 3490 2026
Size: 1096 368 5134
Fragments arranged by size:
5134 1096 368
SacI G_AGCT'C
Cuts at: 2040 3806 2040
Size: 1766 4832
SalI G'TCGA_C
Cuts at: 1936 3940 1936
Size: 2004 4594
SapI GCTCTTCn'nnn_
Cuts at: 2559 2559
Size: 6598
SbfI CC_TGCA'GG
Cuts at: 3950 3950
Size: 6598
ScaI AGT'ACT
Cuts at: 4846 6558 4846
Size: 1712 4886
SexAI A'CCwGG_T
Cuts at: 2495 2495
Size: 6598
SfcI C'TryA_G
Cuts at: 826 1045 2027 2327 2372 2994 3491 3511 3737
Size: 219 982 300 45 622 497 20 226
Cuts at: 3737 3946 5081 5762 5953 826
Size: 209 1135 681 191 1471
Fragments arranged by size:
1471 1135 982 681 622 497 300 226
219 209 191 45 20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 2889 3925 1872
Size: 1017 1036 4545
Fragments arranged by size:
4545 1036 1017
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3007 4702 5603 5865 820
Size: 197 926 1064 1695 901 262 1553
Fragments arranged by size:
1695 1553 1064 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4389 493
Size: 3896 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6598
SphI G_CATG'C
Cuts at: 1132 3956 1132
Size: 2824 3774
SspI AAT'ATT
Cuts at: 5 52 4522 5
Size: 47 4470 2081
Fragments arranged by size:
4470 2081 47
StuI AGG'CCT
Cuts at: 1517 2654 3189 3650 1517
Size: 1137 535 461 4465
Fragments arranged by size:
4465 1137 535 461
StyI C'CwwG_G
Cuts at: 513 1065 2004 2850 3480 4037 513
Size: 552 939 846 630 557 3074
Fragments arranged by size:
3074 939 846 630 557 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 2269 2552 3557 3890 4625 4642
Size: 1255 504 185 283 1005 333 735 17
Cuts at: 4642 4795 4980 5425 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1005 735 504 445 333 283
185 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2490 3095 3537
Size: 274 80 33 51 1443 513 605 442
Cuts at: 3537 4844 6556 96
Size: 1307 1712 138
Fragments arranged by size:
1712 1443 1307 605 513 442 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2324 2356 3041 3073 6558 6590 2324
Size: 32 685 32 3485 32 2332
Fragments arranged by size:
3485 2332 685 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3034 1316
Size: 1718 4880
XbaI T'CTAG_A
Cuts at: 3934 3934
Size: 6598
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6598
XmaI C'CCGG_G
Cuts at: 1870 2887 3923 1870
Size: 1017 1036 4545
Fragments arranged by size:
4545 1036 1017
XmnI GAAnn'nnTTC
Cuts at: 2612 3467 4727 2612
Size: 855 1260 4483
Fragments arranged by size:
4483 1260 855
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4406 276
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI XcmI