Restriction map for
pFN21AB7862
(Circular) (Six-base) MAPSORT of: pFN21AB7862.seq Check: 6985 from: 1 to: 6595
pFN21AB7862 6595 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4405 278
Size: 53 83 186 936 2869 2468
Fragments arranged by size:
2869 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3938 1937
Size: 2001 4594
Acc65I G'GTAC_C
Cuts at: 3916 3916
Size: 6595
AclI AA'CG_TT
Cuts at: 1563 4722 5095 1563
Size: 3159 373 3063
Fragments arranged by size:
3159 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3145 4658 6042 6310 1632
Size: 1513 1513 1384 268 1917
Fragments arranged by size:
1917 1513 1513 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6398
AflIII A'CryG_T
Cuts at: 1203 2503 3187 3271 3859 5494 6471 1203
Size: 1300 684 84 588 1635 977 1327
Fragments arranged by size:
1635 1327 1300 977 684 588 84
AgeI A'CCGG_T
Cuts at: 5426 5426
Size: 6595
AhdI GACnn_n'nnGTC
Cuts at: 5324 5324
Size: 6595
AleI CACnn'nnGTG
Cuts at: 1166 3204 1166
Size: 2038 4557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6595
AlwNI CAG_nnn'CTG
Cuts at: 2032 2463 4338 5910 2032
Size: 431 1875 1572 2717
Fragments arranged by size:
2717 1875 1572 431
ApaLI G'TGCA_C
Cuts at: 4652 5808 4652
Size: 1156 5439
ApoI r'AATT_y
Cuts at: 1493 2531 2550 2563 2796 3111 3363 3904 4130
Size: 1038 19 13 233 315 252 541 226
Cuts at: 4130 4282 6391 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1038 541 315 252 233 226
152 19 13
AseI AT'TA_AT
Cuts at: 160 1032 5149 160
Size: 872 4117 1606
Fragments arranged by size:
4117 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 3920 1870
Size: 73 177 1800 4545
Fragments arranged by size:
4545 1800 177 73
BamHI G'GATC_C
Cuts at: 2152 3925 4292 2152
Size: 1773 367 4455
Fragments arranged by size:
4455 1773 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3916 5371 618
Size: 325 217 627 2129 1455 1842
Fragments arranged by size:
2129 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2121 3914 1391
Size: 730 1793 4072
Fragments arranged by size:
4072 1793 730
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5624
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4786 4820 1903
Size: 34 2849 34 3678
Fragments arranged by size:
3678 2849 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4487 5697 4487
Size: 1210 5385
BclI T'GATC_A
Cuts at: 1548 2578 1548
Size: 1030 5565
BfrBI ATG'CAT
Cuts at: 3845 3845
Size: 6595
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5206 136
Size: 107 122 71 631 605 3534 1525
Fragments arranged by size:
3534 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3194 6590 3194
Size: 3396 3199
BlpI GC'TnA_GC
Cuts at: 4012 4012
Size: 6595
Bme1580I G_kGCm'C
Cuts at: 1790 4656 5812 1790
Size: 2866 1156 2573
Fragments arranged by size:
2866 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6595
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2292 2483 2961
Size: 416 53 83 399 24 875 191 478
Cuts at: 2961 3717 4326 5284 442
Size: 756 609 958 1753
Fragments arranged by size:
1753 958 875 756 609 478 416 399
191 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6595
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 2120 2210 2826 3141 3645 3813 5255
Size: 760 121 90 616 315 504 168 1442
Cuts at: 5255 1239
Size: 2579
Fragments arranged by size:
2579 1442 760 616 504 315 168 121
90
Bpu10I CC'TnA_GC
Cuts at: 2834 2834
Size: 6595
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2424 2569 2847 4720 5585 5883 2424
Size: 145 278 1873 865 298 3136
Fragments arranged by size:
3136 1873 865 298 278 145
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2017 2039 5258 882
Size: 617 518 22 3219 2219
Fragments arranged by size:
3219 2219 617 518 22
BsaAI yAC'GTr
Cuts at: 493 1204 4386 493
Size: 711 3182 2702
Fragments arranged by size:
3182 2702 711
BsaBI GATnn'nnATC
Cuts at: 6589 6589
Size: 6595
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4402 4784 5419 275
Size: 53 83 186 936 2869 382 635 1451
Fragments arranged by size:
2869 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2055 3035 5028 5426 5700 5847 6258 2055
Size: 980 1993 398 274 147 411 2392
Fragments arranged by size:
2392 1993 980 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2050 2080 2231 2261 1178
Size: 30 165 30 647 30 151 30 5512
Fragments arranged by size:
5512 647 165 151 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2074 2399 1186
Size: 888 325 5382
Fragments arranged by size:
5382 888 325
BseYI C'CCAG_C
Cuts at: 2828 5798 6294 2828
Size: 2970 496 3129
Fragments arranged by size:
3129 2970 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6022
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4045 4806 5834 1540
Size: 275 186 2044 761 1028 2301
Fragments arranged by size:
2301 2044 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4656 4741 5812 4656
Size: 85 1071 5439
Fragments arranged by size:
5439 1071 85
BsmI GAATG_Cn'
Cuts at: 3311 4106 4199 3311
Size: 795 93 5707
Fragments arranged by size:
5707 795 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 3914 4656 4741 5812 1391
Size: 399 331 1793 742 85 1071 2174
Fragments arranged by size:
2174 1793 1071 742 399 331 85
BspEI T'CCGG_A
Cuts at: 6258 6258
Size: 6595
BspHI T'CATG_A
Cuts at: 3889 4484 6214 3889
Size: 595 1730 4270
Fragments arranged by size:
4270 1730 595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3950 844
Size: 3106 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4482 1394
Size: 429 2659 3507
Fragments arranged by size:
3507 2659 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3316 5090 5264 65
Size: 1183 2068 1774 174 1396
Fragments arranged by size:
2068 1774 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5239 5426 1700
Size: 110 3429 187 2869
Fragments arranged by size:
3429 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3016 3688 96
Size: 2920 672 3003
Fragments arranged by size:
3003 2920 672
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6595
BssSI C'ACGA_G
Cuts at: 4655 5667 4655
Size: 1012 5583
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6595
BstBI TT'CG_AA
Cuts at: 5399 5399
Size: 6595
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6595
BstYI r'GATC_y
Cuts at: 1972 2152 3194 3925 4292 4677 4694 6146 6232
Size: 180 1042 731 367 385 17 1452 86
Cuts at: 6232 6244 6590 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1042 731 385 367 346 180
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2411 2411
Size: 6595
BtgI C'CryG_G
Cuts at: 513 1065 2049 2133 2273 3076 513
Size: 552 984 84 140 803 4032
Fragments arranged by size:
4032 984 803 552 140 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5856
Fragments arranged by size:
5856 683 56
BtsI GCAGTG_nn'
Cuts at: 2464 4111 4905 4925 2464
Size: 1647 794 20 4134
Fragments arranged by size:
4134 1647 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6595
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3298 3333 529
Size: 35 2734 35 3791
Fragments arranged by size:
3791 2734 35 35
DraI TTT'AAA
Cuts at: 3583 3899 3583
Size: 316 6279
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5602 809
Size: 507 4286 1802
Fragments arranged by size:
4286 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4042 4931 6560
Size: 54 1425 50 275 18 2212 889 1629
Cuts at: 6560 8
Size: 43
Fragments arranged by size:
2212 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4042 1537
Size: 275 2230 4090
Fragments arranged by size:
4090 2230 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4525 1353
Size: 3172 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5182 5556 5702 387
Size: 1064 138 3593 374 146 1280
Fragments arranged by size:
3593 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2120 2210 2826 3141 3145 3645
Size: 393 367 121 90 616 315 4 500
Cuts at: 3645 3813 4658 5255 6042 6310 1239
Size: 168 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 616 597 500 393 367
315 268 168 121 90 4
EcoRI G'AATT_C
Cuts at: 3111 3363 3904 4282 3111
Size: 252 541 378 5424
Fragments arranged by size:
5424 541 378 252
FspI TGC'GCA
Cuts at: 5101 5101
Size: 6595
HaeII r_GCGC'y
Cuts at: 1678 2133 5742 1678
Size: 455 3609 2531
Fragments arranged by size:
3609 2531 455
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2231 2263 2687 2719 5236
Size: 32 135 32 659 32 424 32 2517
Cuts at: 5236 5268 5310 5342 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2517 659 424 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3939 4185 669
Size: 988 281 2001 246 3079
Fragments arranged by size:
3079 2001 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2865 3408 748
Size: 2117 543 3935
Fragments arranged by size:
3935 2117 543
HpaI GTT'AAC
Cuts at: 4185 4185
Size: 6595
KpnI G_GTAC'C
Cuts at: 3920 3920
Size: 6595
MfeI C'AATT_G
Cuts at: 4194 4194
Size: 6595
MluI A'CGCG_T
Cuts at: 6471 6471
Size: 6595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2235 5709 5893 1305
Size: 930 3474 184 2007
Fragments arranged by size:
3474 2007 930 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4773
Fragments arranged by size:
4773 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2968 3052 3087 3204 3220 3288 3304
Size: 648 1802 84 35 117 16 68 16
Cuts at: 3304 3372 3540 4553 4912 5071 518
Size: 68 168 1013 359 159 2042
Fragments arranged by size:
2042 1802 1013 648 359 168 159 117
84 68 68 35 16 16
MspA1I CmG'CkG
Cuts at: 1606 2019 2135 4011 4688 5836 6081 1606
Size: 413 116 1876 677 1148 245 2120
Fragments arranged by size:
2120 1876 1148 677 413 245 116
NcoI C'CATG_G
Cuts at: 513 1065 2273 3076 513
Size: 552 1208 803 4032
Fragments arranged by size:
4032 1208 803 552
NdeI CA'TA_TG
Cuts at: 387 2965 3049 3217 3301 3525 387
Size: 2578 84 168 84 224 3457
Fragments arranged by size:
3457 2578 224 168 84 84
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6595
NotI GC'GGCC_GC
Cuts at: 4042 4042
Size: 6595
NsiI A_TGCA'T
Cuts at: 3847 3847
Size: 6595
NspI r_CATG'y
Cuts at: 1132 1229 2507 3092 3191 3275 3863 3953 5498
Size: 97 1278 585 99 84 588 90 1545
Cuts at: 5498 1132
Size: 2229
Fragments arranged by size:
2229 1545 1278 588 585 99 97 90
84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6595
PciI A'CATG_T
Cuts at: 2503 3187 3271 3859 5494 2503
Size: 684 84 588 1635 3604
Fragments arranged by size:
3604 1635 684 588 84
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6595
PmeI GTTT'AAAC
Cuts at: 3899 3899
Size: 6595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4664 4696 6177 6209 4664
Size: 32 1481 32 5050
Fragments arranged by size:
5050 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6595
PsiI TTA'TAA
Cuts at: 3471 4165 3471
Size: 694 5901
PstI C_TGCA'G
Cuts at: 830 3947 830
Size: 3117 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6595
SacII CC_GC'GG
Cuts at: 2136 2136
Size: 6595
SalI G'TCGA_C
Cuts at: 1936 3937 1936
Size: 2001 4594
SbfI CC_TGCA'GG
Cuts at: 3947 3947
Size: 6595
ScaI AGT'ACT
Cuts at: 4843 6555 4843
Size: 1712 4883
SfcI C'TryA_G
Cuts at: 826 1045 3943 5078 5759 5950 826
Size: 219 2898 1135 681 191 1471
Fragments arranged by size:
2898 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6595
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6595
SmaI CCC'GGG
Cuts at: 1872 3922 1872
Size: 2050 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2439 2584 2862 4699 5600 5862
Size: 197 926 496 145 278 1837 901 262
Cuts at: 5862 820
Size: 1553
Fragments arranged by size:
1837 1553 926 901 496 278 262 197
145
SnaBI TAC'GTA
Cuts at: 493 4386 493
Size: 3893 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6595
SphI G_CATG'C
Cuts at: 1132 3953 1132
Size: 2821 3774
SspI AAT'ATT
Cuts at: 5 52 4519 5
Size: 47 4467 2081
Fragments arranged by size:
4467 2081 47
StuI AGG'CCT
Cuts at: 1517 3597 1517
Size: 2080 4515
StyI C'CwwG_G
Cuts at: 513 1065 2273 3076 4034 513
Size: 552 1208 803 958 3074
Fragments arranged by size:
3074 1208 958 803 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1999 2842 4622 4639 4792 4977 5422
Size: 1255 419 843 1780 17 153 185 445
Cuts at: 5422 325
Size: 1498
Fragments arranged by size:
1780 1498 1255 843 445 419 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3016 3688 4841
Size: 274 80 33 51 1443 1039 672 1153
Cuts at: 4841 6553 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1153 1039 672 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2050 2082 6555 6587 2050
Size: 32 4473 32 2058
Fragments arranged by size:
4473 2058 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2298 1316
Size: 982 5613
XbaI T'CTAG_A
Cuts at: 3931 3931
Size: 6595
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6595
XmaI C'CCGG_G
Cuts at: 1870 3920 1870
Size: 2050 4545
XmnI GAAnn'nnTTC
Cuts at: 2636 3057 3158 3410 3830 4724 2636
Size: 421 101 252 420 894 4507
Fragments arranged by size:
4507 894 421 420 252 101
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4403 276
Size: 53 83 186 936 2869 2468
Fragments arranged by size:
2869 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI