Restriction map for
pFN21AB7860
(Circular) (Six-base) MAPSORT of: pFN21AB7860.seq Check: 2067 from: 1 to: 6502
pFN21AB7860 6502 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3186 3186
Size: 6502
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4312 278
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3845 1937
Size: 1908 4594
Acc65I G'GTAC_C
Cuts at: 3823 3823
Size: 6502
AclI AA'CG_TT
Cuts at: 1563 4629 5002 1563
Size: 3066 373 3063
Fragments arranged by size:
3066 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2060 2160 2750 2811 3315 3527 3556 3683
Size: 428 100 590 61 504 212 29 127
Cuts at: 3683 4565 5949 6217 1632
Size: 882 1384 268 1917
Fragments arranged by size:
1917 1384 882 590 504 428 268 212
127 100 61 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6305
AflIII A'CryG_T
Cuts at: 1203 5401 6378 1203
Size: 4198 977 1327
Fragments arranged by size:
4198 1327 977
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 5231 5231
Size: 6502
AleI CACnn'nnGTG
Cuts at: 1166 3447 1166
Size: 2281 4221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2036 1184
Size: 852 5650
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3104 3136 3104
Size: 32 6470
AlwNI CAG_nnn'CTG
Cuts at: 4245 5817 4245
Size: 1572 4930
ApaLI G'TGCA_C
Cuts at: 3767 4559 5715 3767
Size: 792 1156 4554
Fragments arranged by size:
4554 1156 792
ApoI r'AATT_y
Cuts at: 1493 2446 2597 2624 2894 3401 3811 4037 4189
Size: 953 151 27 270 507 410 226 152
Cuts at: 4189 6298 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 953 507 410 270 226 152
151 27
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 3827 1870
Size: 73 106 1778 4545
Fragments arranged by size:
4545 1778 106 73
BamHI G'GATC_C
Cuts at: 3832 4199 3832
Size: 367 6135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 3823 5278 618
Size: 325 217 627 479 1557 1455 1842
Fragments arranged by size:
1842 1557 1455 627 479 325 217
BanII G_rGCy'C
Cuts at: 1391 2073 3821 1391
Size: 682 1748 4072
Fragments arranged by size:
4072 1748 682
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2148 2461 3033 3104 928
Size: 971 249 313 572 71 4326
Fragments arranged by size:
4326 971 572 313 249 71
BbvCI CC'TCA_GC
Cuts at: 2394 2394
Size: 6502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4693 4727 1903
Size: 34 50 34 2672 34 3678
Fragments arranged by size:
3678 2672 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3238 4394 5604 3238
Size: 1156 1210 4136
Fragments arranged by size:
4136 1210 1156
BclI T'GATC_A
Cuts at: 1548 2928 2992 1548
Size: 1380 64 5058
Fragments arranged by size:
5058 1380 64
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5113 136
Size: 107 122 71 631 605 3441 1525
Fragments arranged by size:
3441 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6497 6497
Size: 6502
BlpI GC'TnA_GC
Cuts at: 2553 3919 2553
Size: 1366 5136
Bme1580I G_kGCm'C
Cuts at: 1790 3771 4563 5719 1790
Size: 1981 792 1156 2573
Fragments arranged by size:
2573 1981 1156 792
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2624 4233 5191
Size: 416 53 83 399 24 1207 1609 958
Cuts at: 5191 442
Size: 1753
Fragments arranged by size:
1753 1609 1207 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2546 2796 3636 5162 1239
Size: 903 404 250 840 1526 2579
Fragments arranged by size:
2579 1526 903 840 404 250
Bpu10I CC'TnA_GC
Cuts at: 2394 2394
Size: 6502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4627 5492 5790 4627
Size: 865 298 5339
Fragments arranged by size:
5339 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3109 5165 882
Size: 617 1610 2056 2219
Fragments arranged by size:
2219 2056 1610 617
BsaAI yAC'GTr
Cuts at: 493 1204 2020 4293 493
Size: 711 816 2273 2702
Fragments arranged by size:
2702 2273 816 711
BsaBI GATnn'nnATC
Cuts at: 6496 6496
Size: 6502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4309 4691 5326 275
Size: 53 83 186 936 2776 382 635 1451
Fragments arranged by size:
2776 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3150 3435 4935 5333 5607 5754 6165 3150
Size: 285 1500 398 274 147 411 3487
Fragments arranged by size:
3487 1500 411 398 285 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2048 3104 3134 3527
Size: 30 165 30 615 30 1056 30 393
Cuts at: 3527 3557 1178
Size: 30 4123
Fragments arranged by size:
4123 1056 615 393 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2411 2603 3125 1186
Size: 1225 192 522 4563
Fragments arranged by size:
4563 1225 522 192
BseYI C'CCAG_C
Cuts at: 2008 5705 6201 2008
Size: 3697 496 2309
Fragments arranged by size:
3697 2309 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3580 1163
Size: 573 1844 4085
Fragments arranged by size:
4085 1844 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3952 4713 5741 1540
Size: 275 186 1951 761 1028 2301
Fragments arranged by size:
2301 1951 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3007 3554 3600 3771 4563 4648 5719 3007
Size: 547 46 171 792 85 1071 3790
Fragments arranged by size:
3790 1071 792 547 171 85 46
BsmI GAATG_Cn'
Cuts at: 2139 3725 4013 4106 2139
Size: 1586 288 93 4535
Fragments arranged by size:
4535 1586 288 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 3007 3554 3600 3771 3821 4563
Size: 399 283 934 547 46 171 50 742
Cuts at: 4563 4648 5719 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 934 742 547 399 283 171
85 50 46
BspEI T'CCGG_A
Cuts at: 3150 3435 6165 3150
Size: 285 2730 3487
Fragments arranged by size:
3487 2730 285
BspHI T'CATG_A
Cuts at: 2260 2860 3274 4391 6121 2260
Size: 600 414 1117 1730 2641
Fragments arranged by size:
2641 1730 1117 600 414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3186 3857 844
Size: 2342 671 3489
Fragments arranged by size:
3489 2342 671
BsrBI CCG'CTC
Cuts at: 1394 1823 4389 1394
Size: 429 2566 3507
Fragments arranged by size:
3507 2566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2567 4997 5171 65
Size: 1183 1319 2430 174 1396
Fragments arranged by size:
2430 1396 1319 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5146 5333 1700
Size: 110 3336 187 2869
Fragments arranged by size:
3336 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2208 96
Size: 2112 4390
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 4562 5574 4562
Size: 1012 5490
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2644 794
Size: 1850 4652
BstBI TT'CG_AA
Cuts at: 5306 5306
Size: 6502
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6502
BstYI r'GATC_y
Cuts at: 1972 2779 3832 4199 4584 4601 6053 6139 6151
Size: 807 1053 367 385 17 1452 86 12
Cuts at: 6151 6497 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1053 807 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2231 3784 513
Size: 552 1166 1553 3231
Fragments arranged by size:
3231 1553 1166 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3237 487
Size: 683 56 2011 3752
Fragments arranged by size:
3752 2011 683 56
BtsI GCAGTG_nn'
Cuts at: 3217 3418 4018 4812 4832 3217
Size: 201 600 794 20 4887
Fragments arranged by size:
4887 794 600 201 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2596 2631 3034 3069 529
Size: 35 2032 35 403 35 3962
Fragments arranged by size:
3962 2032 403 35 35 35
DraI TTT'AAA
Cuts at: 3806 3806
Size: 6502
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3394 3949 4838
Size: 54 1425 50 275 18 1564 555 889
Cuts at: 4838 6467 8
Size: 1629 43
Fragments arranged by size:
1629 1564 1425 889 555 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3949 1537
Size: 275 2137 4090
Fragments arranged by size:
4090 2137 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 2726 4432 1353
Size: 770 603 1706 3423
Fragments arranged by size:
3423 1706 770 603
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5089 5463 5609 387
Size: 1064 138 3500 374 146 1280
Fragments arranged by size:
3500 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2142 2160 2546 2750 2796 2811
Size: 393 428 82 18 386 204 46 15
Cuts at: 2811 3315 3527 3556 3636 3683 4565 5162 5949
Size: 504 212 29 80 47 882 597 787
Cuts at: 5949 6217 1239
Size: 268 1524
Fragments arranged by size:
1524 882 787 597 504 428 393 386
268 212 204 82 80 47 46 29
18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3235 3235
Size: 6502
EcoRI G'AATT_C
Cuts at: 3811 4189 3811
Size: 378 6124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2501 2501
Size: 6502
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 5649 1678
Size: 3971 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2255 2287 2977
Size: 32 135 32 446 32 205 32 690
Cuts at: 2977 3009 3417 3449 3682 3714 5143 5175 5217
Size: 32 408 32 233 32 1429 32 42
Cuts at: 5217 5249 1373
Size: 32 2626
Fragments arranged by size:
2626 1429 690 446 408 233 205 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3846 4092 669
Size: 988 281 1908 246 3079
Fragments arranged by size:
3079 1908 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2792 748
Size: 2044 4458
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 6502
KpnI G_GTAC'C
Cuts at: 3827 3827
Size: 6502
MfeI C'AATT_G
Cuts at: 4101 4101
Size: 6502
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 5616 5800 1305
Size: 960 3351 184 2007
Fragments arranged by size:
3351 2007 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3396 10
Size: 54 1425 343 1564 3116
Fragments arranged by size:
3116 1564 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 3075 3192 3222 3447 4460 4819
Size: 648 852 1057 117 30 225 1013 359
Cuts at: 4819 4978 518
Size: 159 2042
Fragments arranged by size:
2042 1057 1013 852 648 359 225 159
117 30
MspA1I CmG'CkG
Cuts at: 1606 2468 2715 3918 4595 5743 5988 1606
Size: 862 247 1203 677 1148 245 2120
Fragments arranged by size:
2120 1203 1148 862 677 247 245
NcoI C'CATG_G
Cuts at: 513 1065 3784 513
Size: 552 2719 3231
Fragments arranged by size:
3231 2719 552
NdeI CA'TA_TG
Cuts at: 387 2925 387
Size: 2538 3964
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6502
NotI GC'GGCC_GC
Cuts at: 3949 3949
Size: 6502
NspI r_CATG'y
Cuts at: 1132 1229 2017 3860 5405 1132
Size: 97 788 1843 1545 2229
Fragments arranged by size:
2229 1843 1545 788 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6502
PciI A'CATG_T
Cuts at: 5401 5401
Size: 6502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3392 1108
Size: 2284 4218
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3104 3136 4571 4603 6084 6116 3104
Size: 32 1435 32 1481 32 3490
Fragments arranged by size:
3490 1481 1435 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 4072 4072
Size: 6502
PstI C_TGCA'G
Cuts at: 830 3854 830
Size: 3024 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
PvuII CAG'CTG
Cuts at: 2715 2715
Size: 6502
SalI G'TCGA_C
Cuts at: 1936 3844 1936
Size: 1908 4594
SapI GCTCTTCn'nnn_
Cuts at: 2726 2726
Size: 6502
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 4750 6462 4750
Size: 1712 4790
SfcI C'TryA_G
Cuts at: 826 1045 2840 3850 4985 5666 5857 826
Size: 219 1795 1010 1135 681 191 1471
Fragments arranged by size:
1795 1471 1135 1010 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6502
SmaI CCC'GGG
Cuts at: 1872 2051 3829 1872
Size: 179 1778 4545
Fragments arranged by size:
4545 1778 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4606 5507 5769 820
Size: 197 926 2663 901 262 1553
Fragments arranged by size:
2663 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 3317 3569 3588 1517
Size: 1800 252 19 4431
Fragments arranged by size:
4431 1800 252 19
StyI C'CwwG_G
Cuts at: 513 1065 2485 3784 3941 513
Size: 552 1420 1299 157 3074
Fragments arranged by size:
3074 1420 1299 552 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2948 2990 4529 4546 4699 4884 5329
Size: 1255 1368 42 1539 17 153 185 445
Cuts at: 5329 325
Size: 1498
Fragments arranged by size:
1539 1498 1368 1255 445 185 153 42
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 4748 6460
Size: 274 80 33 51 1443 231 2540 1712
Cuts at: 6460 96
Size: 138
Fragments arranged by size:
2540 1712 1443 274 231 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2764 2796 6462 6494 2764
Size: 32 3666 32 2772
Fragments arranged by size:
3666 2772 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6502
XbaI T'CTAG_A
Cuts at: 2450 3838 2450
Size: 1388 5114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3709 3709
Size: 6502
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6502
XmaI C'CCGG_G
Cuts at: 1870 2049 3827 1870
Size: 179 1778 4545
Fragments arranged by size:
4545 1778 179
XmnI GAAnn'nnTTC
Cuts at: 2624 4631 2624
Size: 2007 4495
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4310 276
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI