Restriction map for
pFN21AB7857
(Circular) (Six-base) MAPSORT of: pFN21AB7857.seq Check: 192 from: 1 to: 6364
pFN21AB7857 6364 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2897 4174 278
Size: 53 83 186 936 1361 1277 2468
Fragments arranged by size:
2468 1361 1277 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3707 1937
Size: 1770 4594
Acc65I G'GTAC_C
Cuts at: 3685 3685
Size: 6364
AclI AA'CG_TT
Cuts at: 1563 4491 4864 1563
Size: 2928 373 3063
Fragments arranged by size:
3063 2928 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2869 3037 3205 4427 5811 6079 1632
Size: 1237 168 168 1222 1384 268 1917
Fragments arranged by size:
1917 1384 1237 1222 268 168 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6167
AflIII A'CryG_T
Cuts at: 1203 2943 5263 6240 1203
Size: 1740 2320 977 1327
Fragments arranged by size:
2320 1740 1327 977
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 5093 5093
Size: 6364
AleI CACnn'nnGTG
Cuts at: 1166 2417 3096 1166
Size: 1251 679 4434
Fragments arranged by size:
4434 1251 679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6364
AlwNI CAG_nnn'CTG
Cuts at: 2641 3340 4107 5679 2641
Size: 699 767 1572 3326
Fragments arranged by size:
3326 1572 767 699
ApaI G_GGCC'C
Cuts at: 2077 3635 2077
Size: 1558 4806
ApaLI G'TGCA_C
Cuts at: 2598 3257 4421 5577 2598
Size: 659 1164 1156 3385
Fragments arranged by size:
3385 1164 1156 659
ApoI r'AATT_y
Cuts at: 1493 3673 3899 4051 6160 1493
Size: 2180 226 152 2109 1697
Fragments arranged by size:
2180 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 2397 2510 3628 3689 1870
Size: 73 454 113 1118 61 4545
Fragments arranged by size:
4545 1118 454 113 73 61
BamHI G'GATC_C
Cuts at: 3507 3694 4061 3507
Size: 187 367 5810
Fragments arranged by size:
5810 367 187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2345 2384 2994 3398 3685
Size: 325 217 627 558 39 610 404 287
Cuts at: 3685 5140 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 610 558 404 325 287
217 39
BanII G_rGCy'C
Cuts at: 1391 2055 2077 2181 2724 2813 3233 3618 3635
Size: 664 22 104 543 89 420 385 17
Cuts at: 3635 3683 1391
Size: 48 4072
Fragments arranged by size:
4072 664 543 420 385 104 89 48
22 17
BbeI G_GCGC'C
Cuts at: 3402 3402
Size: 6364
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2323 3530 928
Size: 971 424 1207 3762
Fragments arranged by size:
3762 1207 971 424
BbvCI CC'TCA_GC
Cuts at: 3071 3071
Size: 6364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2782 2816 3370 3404 4555 4589 1903
Size: 34 845 34 554 34 1151 34 3678
Fragments arranged by size:
3678 1151 845 554 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4256 5466 4256
Size: 1210 5154
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 2584 4975
Size: 107 122 71 631 605 400 512 2391
Cuts at: 4975 136
Size: 1525
Fragments arranged by size:
2391 1525 631 605 512 400 122 107
71
BglII A'GATC_T
Cuts at: 6359 6359
Size: 6364
BlpI GC'TnA_GC
Cuts at: 3410 3781 3410
Size: 371 5993
Bme1580I G_kGCm'C
Cuts at: 1790 2077 2602 2951 3261 3635 4425 5581 1790
Size: 287 525 349 310 374 790 1156 2573
Fragments arranged by size:
2573 1156 790 525 374 349 310 287
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6364
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2184 2443 2863
Size: 416 53 83 399 24 767 259 420
Cuts at: 2863 4095 5053 442
Size: 1232 958 1753
Fragments arranged by size:
1753 1232 958 767 420 416 399 259
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2269 2298 2663 5024 1239
Size: 944 86 29 365 2361 2579
Fragments arranged by size:
2579 2361 944 365 86 29
Bpu10I CC'TnA_GC
Cuts at: 2455 2501 2525 2580 3071 2455
Size: 46 24 55 491 5748
Fragments arranged by size:
5748 491 55 46 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2807 4489 5354 5652 2807
Size: 1682 865 298 3519
Fragments arranged by size:
3519 1682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5027 882
Size: 617 3528 2219
Fragments arranged by size:
3528 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4155 493
Size: 711 2951 2702
Fragments arranged by size:
2951 2702 711
BsaBI GATnn'nnATC
Cuts at: 6358 6358
Size: 6364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2517 2576 2894 2936
Size: 53 83 186 936 984 59 318 42
Cuts at: 2936 3103 3399 4171 4553 5188 275
Size: 167 296 772 382 635 1451
Fragments arranged by size:
1451 984 936 772 635 382 318 296
186 167 83 59 53 42
BsaWI w'CCGG_w
Cuts at: 4797 5195 5469 5616 6027 4797
Size: 398 274 147 411 5134
Fragments arranged by size:
5134 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6139
Fragments arranged by size:
6139 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2189 2696 2810 2971 3223 1186
Size: 871 132 507 114 161 252 4327
Fragments arranged by size:
4327 871 507 252 161 132 114
BseYI C'CCAG_C
Cuts at: 2122 2557 2740 2759 3327 3634 5567 6063 2122
Size: 435 183 19 568 307 1933 496 2423
Fragments arranged by size:
2423 1933 568 496 435 307 183 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2700 2809 3326 3536 1163
Size: 573 964 109 517 210 3991
Fragments arranged by size:
3991 964 573 517 210 109
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2659 3015 3298 3814 4575 5603
Size: 275 186 658 356 283 516 761 1028
Cuts at: 5603 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 658 516 356 283 275
186
BsiHKAI G_wGCw'C
Cuts at: 2602 2813 3261 4425 4510 5581 2602
Size: 211 448 1164 85 1071 3385
Fragments arranged by size:
3385 1164 1071 448 211 85
BsiWI C'GTAC_G
Cuts at: 3170 3170
Size: 6364
BsmI GAATG_Cn'
Cuts at: 2783 3423 3875 3968 2783
Size: 640 452 93 5179
Fragments arranged by size:
5179 640 452 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2927 3649 2927
Size: 722 5642
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2077 2181 2602 2724 2813 2951
Size: 399 265 22 104 421 122 89 138
Cuts at: 2951 3233 3261 3618 3635 3683 4425 4510 5581
Size: 282 28 357 17 48 742 85 1071
Cuts at: 5581 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 421 399 357 282 265
138 122 104 89 85 48 28 22
17
BspEI T'CCGG_A
Cuts at: 6027 6027
Size: 6364
BspHI T'CATG_A
Cuts at: 4253 5983 4253
Size: 1730 4634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3719 844
Size: 2875 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2584 2851 3355 4251 1394
Size: 429 761 267 504 896 3507
Fragments arranged by size:
3507 896 761 504 429 267
BsrDI GCAATG_nn'
Cuts at: 65 1248 4859 5033 65
Size: 1183 3611 174 1396
Fragments arranged by size:
3611 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2342 2651 3417 3515 5008 5195 1700
Size: 110 532 309 766 98 1493 187 2869
Fragments arranged by size:
2869 1493 766 532 309 187 110 98
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6364
BssHII G'CGCG_C
Cuts at: 1925 3201 1925
Size: 1276 5088
BssSI C'ACGA_G
Cuts at: 2145 2289 3164 4424 5436 2145
Size: 144 875 1260 1012 3073
Fragments arranged by size:
3073 1260 1012 875 144
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2554 2787 794
Size: 1760 233 4371
Fragments arranged by size:
4371 1760 233
BstBI TT'CG_AA
Cuts at: 2775 5168 2775
Size: 2393 3971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6364
BstXI CCAn_nnnn'nTGG
Cuts at: 3098 3098
Size: 6364
BstYI r'GATC_y
Cuts at: 1972 3087 3507 3694 4061 4446 4463 5915 6001
Size: 1115 420 187 367 385 17 1452 86
Cuts at: 6001 6013 6359 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1115 420 385 367 346 187
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2412 3058 513
Size: 552 939 408 646 3819
Fragments arranged by size:
3819 939 646 552 408
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5625
Fragments arranged by size:
5625 683 56
BtsI GCAGTG_nn'
Cuts at: 2467 3464 3880 4674 4694 2467
Size: 997 416 794 20 4137
Fragments arranged by size:
4137 997 794 416 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6329
DraI TTT'AAA
Cuts at: 3668 3668
Size: 6364
DraIII CAC_nnn'GTG
Cuts at: 3125 3125
Size: 6364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5371 809
Size: 507 4055 1802
Fragments arranged by size:
4055 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2285 2713 3012
Size: 54 1425 50 275 18 455 428 299
Cuts at: 3012 3127 3295 3463 3811 4700 6329 8
Size: 115 168 168 348 889 1629 43
Fragments arranged by size:
1629 1425 889 455 428 348 299 275
168 168 115 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3012 3295 3811 1537
Size: 275 1200 283 516 4090
Fragments arranged by size:
4090 1200 516 283 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2757 2801 3497 4294 1353
Size: 1404 44 696 797 3423
Fragments arranged by size:
3423 1404 797 696 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4951 5325 5471 387
Size: 1064 138 3362 374 146 1280
Fragments arranged by size:
3362 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2811 2811
Size: 6364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2269 2298 2663 2869 3037 3205
Size: 393 551 86 29 365 206 168 168
Cuts at: 3205 4427 5024 5811 6079 1239
Size: 1222 597 787 268 1524
Fragments arranged by size:
1524 1222 787 597 551 393 365 268
206 168 168 86 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2227 2227
Size: 6364
EcoO109I rG'GnC_Cy
Cuts at: 2073 2074 2608 2638 2073
Size: 1 534 30 5799
Fragments arranged by size:
5799 534 30 1
EcoRI G'AATT_C
Cuts at: 3673 4051 3673
Size: 378 5986
FspI TGC'GCA
Cuts at: 3118 4870 3118
Size: 1752 4612
HaeII r_GCGC'y
Cuts at: 1678 2634 3402 3484 5511 1678
Size: 956 768 82 2027 2531
Fragments arranged by size:
2531 2027 956 768 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5005 5037 5079 5111 1373
Size: 32 135 32 3433 32 42 32 2626
Fragments arranged by size:
3433 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3708 3954 669
Size: 988 281 1770 246 3079
Fragments arranged by size:
3079 1770 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6364
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KasI G'GCGC_C
Cuts at: 3398 3398
Size: 6364
KpnI G_GTAC'C
Cuts at: 3689 3689
Size: 6364
MfeI C'AATT_G
Cuts at: 3963 3963
Size: 6364
MluI A'CGCG_T
Cuts at: 2943 6240 2943
Size: 3297 3067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2182 2838 3090 3342 5478 5662 1305
Size: 877 656 252 252 2136 184 2007
Fragments arranged by size:
2136 2007 877 656 252 252 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2287 2715 10
Size: 54 1425 343 455 428 3659
Fragments arranged by size:
3659 1425 455 428 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 3096 4322 4681 4840 518
Size: 648 1251 679 1226 359 159 2042
Fragments arranged by size:
2042 1251 1226 679 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2928 3030 3366 3656 3780 4457 5605 5850
Size: 1322 102 336 290 124 677 1148 245
Cuts at: 5850 1606
Size: 2120
Fragments arranged by size:
2120 1322 1148 677 336 290 245 124
102
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 6364
NarI GG'CG_CC
Cuts at: 3399 3399
Size: 6364
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4873
Fragments arranged by size:
4873 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 6364
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6364
NotI GC'GGCC_GC
Cuts at: 3295 3811 3295
Size: 516 5848
NspI r_CATG'y
Cuts at: 1132 1229 2552 3722 5267 1132
Size: 97 1323 1170 1545 2229
Fragments arranged by size:
2229 1545 1323 1170 97
PasI CC'CwG_GG
Cuts at: 1348 2181 2612 1348
Size: 833 431 5100
Fragments arranged by size:
5100 833 431
PciI A'CATG_T
Cuts at: 5263 5263
Size: 6364
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2641 1108
Size: 1533 4831
PfoI T'CCnGG_A
Cuts at: 2055 2213 2246 2055
Size: 158 33 6173
Fragments arranged by size:
6173 158 33
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 5946 5978 4433
Size: 32 1481 32 4819
Fragments arranged by size:
4819 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2608 2638 2608
Size: 30 6334
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6364
PsiI TTA'TAA
Cuts at: 3934 3934
Size: 6364
PspOMI G'GGCC_C
Cuts at: 2073 3631 2073
Size: 1558 4806
PstI C_TGCA'G
Cuts at: 830 2373 3716 830
Size: 1543 1343 3478
Fragments arranged by size:
3478 1543 1343
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
SacI G_AGCT'C
Cuts at: 2813 2813
Size: 6364
SalI G'TCGA_C
Cuts at: 1936 3706 1936
Size: 1770 4594
SapI GCTCTTCn'nnn_
Cuts at: 2757 2801 2757
Size: 44 6320
SbfI CC_TGCA'GG
Cuts at: 3716 3716
Size: 6364
ScaI AGT'ACT
Cuts at: 4612 6324 4612
Size: 1712 4652
SfcI C'TryA_G
Cuts at: 826 1045 2224 2369 3712 4847 5528 5719 826
Size: 219 1179 145 1343 1135 681 191 1471
Fragments arranged by size:
1471 1343 1179 1135 681 219 191 145
SfoI GGC'GCC
Cuts at: 3400 3400
Size: 6364
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 3417 1700
Size: 1717 4647
SmaI CCC'GGG
Cuts at: 1872 3691 1872
Size: 1819 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2822 4468 5369 5631 820
Size: 197 926 879 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 879 262 197
SnaBI TAC'GTA
Cuts at: 493 4155 493
Size: 3662 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 2552 3722 1132
Size: 1420 1170 3774
Fragments arranged by size:
3774 1420 1170
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 2506 2882 2966 3050 3386 1517
Size: 989 376 84 84 336 4495
Fragments arranged by size:
4495 989 376 336 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 3393 3803 513
Size: 552 939 1389 410 3074
Fragments arranged by size:
3074 1389 939 552 410
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2666 2852 3341 4391 4408 4561 4746
Size: 1255 1086 186 489 1050 17 153 185
Cuts at: 4746 5191 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1086 1050 489 445 186 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2028 4610 6322
Size: 274 80 33 51 1443 51 2582 1712
Cuts at: 6322 96
Size: 138
Fragments arranged by size:
2582 1712 1443 274 138 80 51 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2280 2312 2592 2624 6324 6356 2280
Size: 32 280 32 3700 32 2288
Fragments arranged by size:
3700 2288 280 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6364
XbaI T'CTAG_A
Cuts at: 3700 3700
Size: 6364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2165 2165
Size: 6364
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6364
XmaI C'CCGG_G
Cuts at: 1870 3689 1870
Size: 1819 4545
XmnI GAAnn'nnTTC
Cuts at: 2771 3023 3191 3275 3443 4493 2771
Size: 252 168 84 168 1050 4642
Fragments arranged by size:
4642 1050 252 168 168 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2895 4172 276
Size: 53 83 186 936 1361 1277 2468
Fragments arranged by size:
2468 1361 1277 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SrfI SwaI