Restriction map for
pFN21AB7856
(Circular) (Six-base) MAPSORT of: pFN21AB7856.seq Check: 9009 from: 1 to: 6586
pFN21AB7856 6586 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4396 278
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2357 3929 1937
Size: 420 1572 4594
Fragments arranged by size:
4594 1572 420
Acc65I G'GTAC_C
Cuts at: 3907 3907
Size: 6586
AclI AA'CG_TT
Cuts at: 1563 3873 4713 5086 1563
Size: 2310 840 373 3063
Fragments arranged by size:
3063 2310 840 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2425 2540 2775 4649 6033 6301 1632
Size: 793 115 235 1874 1384 268 1917
Fragments arranged by size:
1917 1874 1384 793 268 235 115
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6389
AflIII A'CryG_T
Cuts at: 1203 2463 2674 2680 2721 2807 5485 6462 1203
Size: 1260 211 6 41 86 2678 977 1327
Fragments arranged by size:
2678 1327 1260 977 211 86 41 6
AgeI A'CCGG_T
Cuts at: 5417 5417
Size: 6586
AhdI GACnn_n'nnGTC
Cuts at: 5315 5315
Size: 6586
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2583 1184
Size: 1399 5187
AlwNI CAG_nnn'CTG
Cuts at: 2353 4329 5901 2353
Size: 1976 1572 3038
Fragments arranged by size:
3038 1976 1572
ApaLI G'TGCA_C
Cuts at: 4643 5799 4643
Size: 1156 5430
ApoI r'AATT_y
Cuts at: 1493 2865 3033 3344 3789 3833 3895 4121 4273
Size: 1372 168 311 445 44 62 226 152
Cuts at: 4273 6382 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1372 445 311 226 168 152
62 44
AseI AT'TA_AT
Cuts at: 160 1032 5140 160
Size: 872 4108 1606
Fragments arranged by size:
4108 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
AvaI C'yCGr_G
Cuts at: 1870 1943 3911 1870
Size: 73 1968 4545
Fragments arranged by size:
4545 1968 73
BamHI G'GATC_C
Cuts at: 3916 4283 3916
Size: 367 6219
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 3907 5362 618
Size: 325 217 627 404 1716 1455 1842
Fragments arranged by size:
1842 1716 1455 627 404 325 217
BanII G_rGCy'C
Cuts at: 1391 2843 3599 3767 3905 1391
Size: 1452 756 168 138 4072
Fragments arranged by size:
4072 1452 756 168 138
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2084 2273 928
Size: 971 185 189 5241
Fragments arranged by size:
5241 971 189 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4777 4811 1903
Size: 34 2840 34 3678
Fragments arranged by size:
3678 2840 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4478 5688 4478
Size: 1210 5376
BclI T'GATC_A
Cuts at: 1548 2770 1548
Size: 1222 5364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5197 136
Size: 107 122 71 631 605 337 3188 1525
Fragments arranged by size:
3188 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2714 3260 3302 6581 2714
Size: 546 42 3279 2719
Fragments arranged by size:
3279 2719 546 42
BlpI GC'TnA_GC
Cuts at: 4003 4003
Size: 6586
Bme1580I G_kGCm'C
Cuts at: 1790 4647 5803 1790
Size: 2857 1156 2573
Fragments arranged by size:
2857 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2059 1318
Size: 741 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2288 2650 4317
Size: 416 53 83 399 24 871 362 1667
Cuts at: 4317 5275 442
Size: 958 1753
Fragments arranged by size:
1753 1667 958 871 416 399 362 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6586
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2229 2229
Size: 6586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2193 2895 2979 3315 3483 3567 3583
Size: 917 37 702 84 336 168 84 16
Cuts at: 3583 5246 1239
Size: 1663 2579
Fragments arranged by size:
2579 1663 917 702 336 168 84 84
37 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2320 4711 5576 5874 2320
Size: 2391 865 298 3032
Fragments arranged by size:
3032 2391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2691 5249 882
Size: 617 1192 2558 2219
Fragments arranged by size:
2558 2219 1192 617
BsaAI yAC'GTr
Cuts at: 493 1204 2681 4377 493
Size: 711 1477 1696 2702
Fragments arranged by size:
2702 1696 1477 711
BsaBI GATnn'nnATC
Cuts at: 6580 6580
Size: 6586
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4393 4775 5410 275
Size: 53 83 186 936 2860 382 635 1451
Fragments arranged by size:
2860 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3195 3629 3713 5019 5417 5691 5838 6249 3195
Size: 434 84 1306 398 274 147 411 3532
Fragments arranged by size:
3532 1306 434 411 398 274 147 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2637 2667 1178
Size: 30 165 30 1234 30 5097
Fragments arranged by size:
5097 1234 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 1186
Size: 1015 5571
BseYI C'CCAG_C
Cuts at: 5789 6285 5789
Size: 496 6090
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2464 2625 2746 2998 3082 3166 3418
Size: 573 728 161 121 252 84 84 252
Cuts at: 3418 3670 1163
Size: 252 4079
Fragments arranged by size:
4079 728 573 252 252 252 161 121
84 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 4036 4797 5825 1540
Size: 275 186 15 2020 761 1028 2301
Fragments arranged by size:
2301 2020 1028 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2210 2843 3599 3767 4647 4732 5803 2210
Size: 633 756 168 880 85 1071 2993
Fragments arranged by size:
2993 1071 880 756 633 168 85
BsmI GAATG_Cn'
Cuts at: 3737 4097 4190 3737
Size: 360 93 6133
Fragments arranged by size:
6133 360 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2210 2843 3599 3767 3905 4647 4732
Size: 399 420 633 756 168 138 742 85
Cuts at: 4732 5803 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 756 742 633 420 399 168
138 85
BspEI T'CCGG_A
Cuts at: 6249 6249
Size: 6586
BspHI T'CATG_A
Cuts at: 3307 3316 4475 6205 3307
Size: 9 1159 1730 3688
Fragments arranged by size:
3688 1730 1159 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3941 844
Size: 3097 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4473 1394
Size: 429 2650 3507
Fragments arranged by size:
3507 2650 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2455 3574 5081 5255 65
Size: 1183 1207 1119 1507 174 1396
Fragments arranged by size:
1507 1396 1207 1183 1119 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5230 5417 1700
Size: 110 3420 187 2869
Fragments arranged by size:
3420 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3358 96
Size: 3262 3324
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6586
BssSI C'ACGA_G
Cuts at: 4646 5658 4646
Size: 1012 5574
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6586
BstBI TT'CG_AA
Cuts at: 5390 5390
Size: 6586
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6586
BstYI r'GATC_y
Cuts at: 1972 2714 3260 3302 3916 4283 4668 4685 6137
Size: 742 546 42 614 367 385 17 1452
Cuts at: 6137 6223 6235 6581 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 742 614 546 385 367 346
86 42 17 12
BstZ17I GTA'TAC
Cuts at: 2358 2358
Size: 6586
Bsu36I CC'TnA_GG
Cuts at: 2244 2244
Size: 6586
BtgI C'CryG_G
Cuts at: 513 1065 2004 2341 513
Size: 552 939 337 4758
Fragments arranged by size:
4758 939 552 337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5847
Fragments arranged by size:
5847 683 56
BtsI GCAGTG_nn'
Cuts at: 2613 2734 2986 3070 3154 3406 3658 3742 4102
Size: 121 252 84 84 252 252 84 360
Cuts at: 4102 4896 4916 2613
Size: 794 20 4283
Fragments arranged by size:
4283 794 360 252 252 252 121 84
84 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6586
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3556 3591 529
Size: 35 2992 35 3524
Fragments arranged by size:
3524 2992 35 35
DraI TTT'AAA
Cuts at: 3890 3890
Size: 6586
DraIII CAC_nnn'GTG
Cuts at: 2128 2128
Size: 6586
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5593 809
Size: 507 4277 1802
Fragments arranged by size:
4277 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 4033 4922
Size: 54 1425 50 275 18 183 2020 889
Cuts at: 4922 6551 8
Size: 1629 43
Fragments arranged by size:
2020 1629 1425 889 275 183 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 4033 1537
Size: 275 201 2020 4090
Fragments arranged by size:
4090 2020 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 3697 4516 1353
Size: 2344 819 3423
Fragments arranged by size:
3423 2344 819
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5173 5547 5693 387
Size: 1064 138 3584 374 146 1280
Fragments arranged by size:
3584 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2841 3597 3765 2841
Size: 756 168 5662
Fragments arranged by size:
5662 756 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2193 2425 2540 2775 2895 2979
Size: 393 524 37 232 115 235 120 84
Cuts at: 2979 3315 3483 3567 3583 4649 5246 6033 6301
Size: 336 168 84 16 1066 597 787 268
Cuts at: 6301 1239
Size: 1524
Fragments arranged by size:
1524 1066 787 597 524 393 336 268
235 232 168 120 115 84 84 37
16
EcoO109I rG'GnC_Cy
Cuts at: 2248 3218 2248
Size: 970 5616
EcoRI G'AATT_C
Cuts at: 2865 3033 3789 3895 4273 2865
Size: 168 756 106 378 5178
Fragments arranged by size:
5178 756 378 168 106
FspI TGC'GCA
Cuts at: 5092 5092
Size: 6586
HaeII r_GCGC'y
Cuts at: 1678 5733 1678
Size: 4055 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5227 5259 5301 5333 1373
Size: 32 135 32 3655 32 42 32 2626
Fragments arranged by size:
3655 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3930 4176 669
Size: 988 281 1992 246 3079
Fragments arranged by size:
3079 1992 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2658 748
Size: 1910 4676
HpaI GTT'AAC
Cuts at: 4176 4176
Size: 6586
KpnI G_GTAC'C
Cuts at: 3911 3911
Size: 6586
MfeI C'AATT_G
Cuts at: 4185 4185
Size: 6586
MluI A'CGCG_T
Cuts at: 6462 6462
Size: 6586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2407 3298 3641 3876 5700 5884 1305
Size: 1102 891 343 235 1824 184 2007
Fragments arranged by size:
2007 1824 1102 891 343 235 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4764
Fragments arranged by size:
4764 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2679 2892 3321 3394 4544 4903 5062
Size: 648 1513 213 429 73 1150 359 159
Cuts at: 5062 518
Size: 2042
Fragments arranged by size:
2042 1513 1150 648 429 359 213 159
73
MspA1I CmG'CkG
Cuts at: 1606 2221 2478 4002 4679 5827 6072 1606
Size: 615 257 1524 677 1148 245 2120
Fragments arranged by size:
2120 1524 1148 677 615 257 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2341 513
Size: 552 939 337 4758
Fragments arranged by size:
4758 939 552 337
NdeI CA'TA_TG
Cuts at: 387 3391 387
Size: 3004 3582
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6586
NotI GC'GGCC_GC
Cuts at: 4033 4033
Size: 6586
NspI r_CATG'y
Cuts at: 1132 1229 2373 2426 2467 2678 2725 2811 3944
Size: 97 1144 53 41 211 47 86 1133
Cuts at: 3944 5489 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1144 1133 211 97 86 53
47 41
PasI CC'CwG_GG
Cuts at: 1348 2035 2161 1348
Size: 687 126 5773
Fragments arranged by size:
5773 687 126
PciI A'CATG_T
Cuts at: 2463 2674 2721 2807 5485 2463
Size: 211 47 86 2678 3564
Fragments arranged by size:
3564 2678 211 86 47
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6586
PfoI T'CCnGG_A
Cuts at: 2075 2075
Size: 6586
PmeI GTTT'AAAC
Cuts at: 3890 3890
Size: 6586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4655 4687 6168 6200 4655
Size: 32 1481 32 5041
Fragments arranged by size:
5041 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6586
PsiI TTA'TAA
Cuts at: 4156 4156
Size: 6586
PstI C_TGCA'G
Cuts at: 830 3938 830
Size: 3108 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6586
PvuII CAG'CTG
Cuts at: 2478 2478
Size: 6586
SacI G_AGCT'C
Cuts at: 2843 3599 3767 2843
Size: 756 168 5662
Fragments arranged by size:
5662 756 168
SalI G'TCGA_C
Cuts at: 1936 3928 1936
Size: 1992 4594
SbfI CC_TGCA'GG
Cuts at: 3938 3938
Size: 6586
ScaI AGT'ACT
Cuts at: 4834 6546 4834
Size: 1712 4874
SfcI C'TryA_G
Cuts at: 826 1045 2758 2830 3934 5069 5750 5941 826
Size: 219 1713 72 1104 1135 681 191 1471
Fragments arranged by size:
1713 1471 1135 1104 681 219 191 72
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6586
SmaI CCC'GGG
Cuts at: 1872 3913 1872
Size: 2041 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2299 4690 5591 5853 820
Size: 197 926 356 2391 901 262 1553
Fragments arranged by size:
2391 1553 926 901 356 262 197
SnaBI TAC'GTA
Cuts at: 493 4377 493
Size: 3884 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6586
SphI G_CATG'C
Cuts at: 1132 3944 1132
Size: 2812 3774
SspI AAT'ATT
Cuts at: 5 52 4510 5
Size: 47 4458 2081
Fragments arranged by size:
4458 2081 47
StuI AGG'CCT
Cuts at: 1517 2968 3052 3136 3472 3556 3640 3724 3808
Size: 1451 84 84 336 84 84 84 84
Cuts at: 3808 1517
Size: 4295
Fragments arranged by size:
4295 1451 336 84 84 84 84 84
84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2329 2341 4025 513
Size: 552 939 325 12 1684 3074
Fragments arranged by size:
3074 1684 939 552 325 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 4613 4630 4783 4968 5413 325
Size: 1255 573 2460 17 153 185 445 1498
Fragments arranged by size:
2460 1498 1255 573 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3358 4832 6544
Size: 274 80 33 51 1443 1381 1474 1712
Cuts at: 6544 96
Size: 138
Fragments arranged by size:
1712 1474 1443 1381 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2637 2669 6546 6578 2637
Size: 32 3877 32 2645
Fragments arranged by size:
3877 2645 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6586
XbaI T'CTAG_A
Cuts at: 3922 3922
Size: 6586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 2605
Size: 6586
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6586
XmaI C'CCGG_G
Cuts at: 1870 3911 1870
Size: 2041 4545
XmnI GAAnn'nnTTC
Cuts at: 2438 2504 2531 2660 4715 2438
Size: 66 27 129 2055 4309
Fragments arranged by size:
4309 2055 129 66 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4394 276
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI