Restriction map for
pFN21AB7855
(Circular) (Six-base) MAPSORT of: pFN21AB7855.seq Check: 5241 from: 1 to: 6358
pFN21AB7855 6358 bp
With 182 enzymes: SgfI *
May 13, 2009 17:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3283 3283
Size: 6358
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4168 278
Size: 53 83 186 936 2632 2468
Fragments arranged by size:
2632 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3411 3701 1937
Size: 1474 290 4594
Fragments arranged by size:
4594 1474 290
Acc65I G'GTAC_C
Cuts at: 3679 3679
Size: 6358
AclI AA'CG_TT
Cuts at: 1563 4485 4858 1563
Size: 2922 373 3063
Fragments arranged by size:
3063 2922 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4421 5805 6073 1632
Size: 2789 1384 268 1917
Fragments arranged by size:
2789 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6161
AflIII A'CryG_T
Cuts at: 1203 5257 6234 1203
Size: 4054 977 1327
Fragments arranged by size:
4054 1327 977
AgeI A'CCGG_T
Cuts at: 5189 5189
Size: 6358
AhdI GACnn_n'nnGTC
Cuts at: 5087 5087
Size: 6358
AleI CACnn'nnGTG
Cuts at: 1166 3101 1166
Size: 1935 4423
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3017 1184
Size: 1833 4525
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 6326
AlwNI CAG_nnn'CTG
Cuts at: 2350 2869 3023 3182 3230 3433 3436 3548 4101
Size: 519 154 159 48 203 3 112 553
Cuts at: 4101 5673 2350
Size: 1572 3035
Fragments arranged by size:
3035 1572 553 519 203 159 154 112
48 3
ApaI G_GGCC'C
Cuts at: 3526 3565 3526
Size: 39 6319
ApaLI G'TGCA_C
Cuts at: 2735 4415 5571 2735
Size: 1680 1156 3522
Fragments arranged by size:
3522 1680 1156
ApoI r'AATT_y
Cuts at: 1493 2858 3667 3893 4045 6154 1493
Size: 1365 809 226 152 2109 1697
Fragments arranged by size:
2109 1697 1365 809 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4912 160
Size: 872 3880 1606
Fragments arranged by size:
3880 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
AvaI C'yCGr_G
Cuts at: 1870 1943 2709 3565 3683 1870
Size: 73 766 856 118 4545
Fragments arranged by size:
4545 856 766 118 73
BamHI G'GATC_C
Cuts at: 3688 4055 3688
Size: 367 5991
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3679 5134 618
Size: 325 217 627 1892 1455 1842
Fragments arranged by size:
1892 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2316 2586 2844 2853 3477 3526 3565 3573
Size: 925 270 258 9 624 49 39 8
Cuts at: 3573 3677 1391
Size: 104 4072
Fragments arranged by size:
4072 925 624 270 258 104 49 39
9 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3354 3606 928
Size: 971 1455 252 3680
Fragments arranged by size:
3680 1455 971 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2450 2484 2605 2639 4549 4583 1903
Size: 34 513 34 121 34 1910 34 3678
Fragments arranged by size:
3678 1910 513 121 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4250 5460 4250
Size: 1210 5148
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2295 4969 136
Size: 107 122 71 631 605 623 2674 1525
Fragments arranged by size:
2674 1525 631 623 605 122 107 71
BglII A'GATC_T
Cuts at: 2589 2637 6353 2589
Size: 48 3716 2594
Fragments arranged by size:
3716 2594 48
BlpI GC'TnA_GC
Cuts at: 2310 3016 3775 2310
Size: 706 759 4893
Fragments arranged by size:
4893 759 706
Bme1580I G_kGCm'C
Cuts at: 1790 2569 2739 3526 3565 4419 5575 1790
Size: 779 170 787 39 854 1156 2573
Fragments arranged by size:
2573 1156 854 787 779 170 39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6358
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3230 3566 4089
Size: 416 53 83 399 24 1813 336 523
Cuts at: 4089 5047 442
Size: 958 1753
Fragments arranged by size:
1813 1753 958 523 416 399 336 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6358
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 6358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 3159 3177 5018 1239
Size: 1163 757 18 1841 2579
Fragments arranged by size:
2579 1841 1163 757 18
Bpu10I CC'TnA_GC
Cuts at: 3068 3068
Size: 6358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2570 4483 5348 5646 2570
Size: 1913 865 298 3282
Fragments arranged by size:
3282 1913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2918 5021 882
Size: 617 1419 2103 2219
Fragments arranged by size:
2219 2103 1419 617
BsaAI yAC'GTr
Cuts at: 493 1204 4149 493
Size: 711 2945 2702
Fragments arranged by size:
2945 2702 711
BsaBI GATnn'nnATC
Cuts at: 6352 6352
Size: 6358
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4165 4547 5182 275
Size: 53 83 186 936 2632 382 635 1451
Fragments arranged by size:
2632 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2760 2892 4791 5189 5463 5610 6021 2760
Size: 132 1899 398 274 147 411 3097
Fragments arranged by size:
3097 1899 411 398 274 147 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3593 3623 1178
Size: 30 165 30 2190 30 3913
Fragments arranged by size:
3913 2190 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2167 2238 2915 3246 3598 1186
Size: 981 71 677 331 352 3946
Fragments arranged by size:
3946 981 677 352 331 71
BseYI C'CCAG_C
Cuts at: 2700 2783 3042 3223 3296 3382 3541 5561 6057
Size: 83 259 181 73 86 159 2020 496
Cuts at: 6057 2700
Size: 3001
Fragments arranged by size:
3001 2020 496 259 181 159 86 83
73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2681 1163
Size: 573 945 4840
Fragments arranged by size:
4840 945 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2836 2887 3808 4569 5597 1540
Size: 275 186 835 51 921 761 1028 2301
Fragments arranged by size:
2301 1028 921 835 761 275 186 51
BsiHKAI G_wGCw'C
Cuts at: 2159 2316 2586 2739 2844 3074 4419 4504 5575
Size: 157 270 153 105 230 1345 85 1071
Cuts at: 5575 2159
Size: 2942
Fragments arranged by size:
2942 1345 1071 270 230 157 153 105
85
BsmI GAATG_Cn'
Cuts at: 2473 3869 3962 2473
Size: 1396 93 4869
Fragments arranged by size:
4869 1396 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2316 2569 2586 2739 2844 2853
Size: 399 369 157 253 17 153 105 9
Cuts at: 2853 3074 3477 3526 3565 3573 3677 4419 4504
Size: 221 403 49 39 8 104 742 85
Cuts at: 4504 5575 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 369 253 221
157 153 105 104 85 49 39 17
9 8
BspEI T'CCGG_A
Cuts at: 2760 6021 2760
Size: 3261 3097
BspHI T'CATG_A
Cuts at: 2940 4247 5977 2940
Size: 1307 1730 3321
Fragments arranged by size:
3321 1730 1307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2877 3283 3713 844
Size: 2033 406 430 3489
Fragments arranged by size:
3489 2033 430 406
BsrBI CCG'CTC
Cuts at: 1394 1823 4245 1394
Size: 429 2422 3507
Fragments arranged by size:
3507 2422 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2994 3612 4853 5027 65
Size: 1183 1746 618 1241 174 1396
Fragments arranged by size:
1746 1396 1241 1183 618 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2253 5002 5189 1700
Size: 110 443 2749 187 2869
Fragments arranged by size:
2869 2749 443 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6358
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6358
BssSI C'ACGA_G
Cuts at: 2267 4418 5430 2267
Size: 2151 1012 3195
Fragments arranged by size:
3195 2151 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6358
BstBI TT'CG_AA
Cuts at: 5162 5162
Size: 6358
BstEII G'GTnAC_C
Cuts at: 1184 2233 1184
Size: 1049 5309
BstXI CCAn_nnnn'nTGG
Cuts at: 2054 3484 2054
Size: 1430 4928
BstYI r'GATC_y
Cuts at: 1972 2589 2637 3688 4055 4440 4457 5909 5995
Size: 617 48 1051 367 385 17 1452 86
Cuts at: 5995 6007 6353 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1051 617 385 367 346 86
48 17 12
Bsu36I CC'TnA_GG
Cuts at: 3258 3495 3258
Size: 237 6121
BtgI C'CryG_G
Cuts at: 513 1065 2004 2130 2853 3099 3198 513
Size: 552 939 126 723 246 99 3673
Fragments arranged by size:
3673 939 723 552 246 126 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5619
Fragments arranged by size:
5619 683 56
BtsI GCAGTG_nn'
Cuts at: 2574 3874 4668 4688 2574
Size: 1300 794 20 4244
Fragments arranged by size:
4244 1300 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6323
DraI TTT'AAA
Cuts at: 3662 3662
Size: 6358
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5365 809
Size: 507 4049 1802
Fragments arranged by size:
4049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2748 3805 4694
Size: 54 1425 50 275 18 918 1057 889
Cuts at: 4694 6323 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1057 918 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3805 1537
Size: 275 1993 4090
Fragments arranged by size:
4090 1993 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 2942 4288 1353
Size: 1083 506 1346 3423
Fragments arranged by size:
3423 1346 1083 506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2513 4945 5319 5465 387
Size: 1064 138 924 2432 374 146 1280
Fragments arranged by size:
2432 1280 1064 924 374 146 138
EcoICRI GAG'CTC
Cuts at: 2314 2584 2842 2314
Size: 270 258 5830
Fragments arranged by size:
5830 270 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2402 3159 3177 4421 5018 5805 6073
Size: 393 770 757 18 1244 597 787 268
Cuts at: 6073 1239
Size: 1524
Fragments arranged by size:
1524 1244 787 770 757 597 393 268
18
EcoNI CCTnn'n_nnAGG
Cuts at: 2384 3167 2384
Size: 783 5575
EcoO109I rG'GnC_Cy
Cuts at: 2242 2377 2503 2517 3220 3251 3319 3357 3522
Size: 135 126 14 703 31 68 38 165
Cuts at: 3522 3531 3561 3562 2242
Size: 9 30 1 5038
Fragments arranged by size:
5038 703 165 135 126 68 38 31
30 14 9 1
EcoRI G'AATT_C
Cuts at: 2858 3667 4045 2858
Size: 809 378 5171
Fragments arranged by size:
5171 809 378
FspI TGC'GCA
Cuts at: 2222 4864 2222
Size: 2642 3716
FspAI rTGC'GCAy
Cuts at: 2222 2222
Size: 6358
HaeII r_GCGC'y
Cuts at: 1678 2128 5505 1678
Size: 450 3377 2531
Fragments arranged by size:
3377 2531 450
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3389 3421 3593 3625 4999
Size: 32 135 32 1817 32 172 32 1374
Cuts at: 4999 5031 5073 5105 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1817 1374 172 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3412 3702 3948 669
Size: 988 281 1474 290 246 3079
Fragments arranged by size:
3079 1474 988 290 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6358
HpaI GTT'AAC
Cuts at: 3948 3948
Size: 6358
KpnI G_GTAC'C
Cuts at: 3683 3683
Size: 6358
MfeI C'AATT_G
Cuts at: 3957 3957
Size: 6358
MluI A'CGCG_T
Cuts at: 6234 6234
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2856 5472 5656 1305
Size: 1551 2616 184 2007
Fragments arranged by size:
2616 2007 1551 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2750 10
Size: 54 1425 343 918 3618
Fragments arranged by size:
3618 1425 918 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 2999 3101 3452 3482 4316 4675
Size: 648 1512 321 102 351 30 834 359
Cuts at: 4675 4834 518
Size: 159 2042
Fragments arranged by size:
2042 1512 834 648 359 351 321 159
102 30
MspA1I CmG'CkG
Cuts at: 1606 2092 2132 2328 2619 2818 2961 3331 3386
Size: 486 40 196 291 199 143 370 55
Cuts at: 3386 3433 3774 4451 5599 5844 1606
Size: 47 341 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 486 370 341 291 245
199 196 143 55 47 40
NcoI C'CATG_G
Cuts at: 513 1065 2004 2853 3099 3198 513
Size: 552 939 849 246 99 3673
Fragments arranged by size:
3673 939 849 552 246 99
NdeI CA'TA_TG
Cuts at: 387 2920 387
Size: 2533 3825
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6358
NotI GC'GGCC_GC
Cuts at: 3805 3805
Size: 6358
NspI r_CATG'y
Cuts at: 1132 1229 2101 3716 5261 1132
Size: 97 872 1615 1545 2229
Fragments arranged by size:
2229 1615 1545 872 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6358
PciI A'CATG_T
Cuts at: 5257 5257
Size: 6358
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 1108
Size: 1242 5116
PmeI GTTT'AAAC
Cuts at: 3662 3662
Size: 6358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2594 2626 4427 4459 5940 5972 2594
Size: 32 1801 32 1481 32 2980
Fragments arranged by size:
2980 1801 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 2377 2503 2242
Size: 135 126 6097
Fragments arranged by size:
6097 135 126
PshAI GACnn'nnGTC
Cuts at: 1613 2886 1613
Size: 1273 5085
PsiI TTA'TAA
Cuts at: 3928 3928
Size: 6358
PspOMI G'GGCC_C
Cuts at: 3522 3561 3522
Size: 39 6319
PstI C_TGCA'G
Cuts at: 830 2793 3330 3336 3710 830
Size: 1963 537 6 374 3478
Fragments arranged by size:
3478 1963 537 374 6
PvuI CG_AT'CG
Cuts at: 2001 2887 2001
Size: 886 5472
PvuII CAG'CTG
Cuts at: 2619 2818 3331 3386 3433 2619
Size: 199 513 55 47 5544
Fragments arranged by size:
5544 513 199 55 47
RsrII CG'GwC_CG
Cuts at: 2323 2323
Size: 6358
SacI G_AGCT'C
Cuts at: 2316 2586 2844 2316
Size: 270 258 5830
Fragments arranged by size:
5830 270 258
SacII CC_GC'GG
Cuts at: 2133 2133
Size: 6358
SalI G'TCGA_C
Cuts at: 1936 3410 3700 1936
Size: 1474 290 4594
Fragments arranged by size:
4594 1474 290
SanDI GG'GwC_CC
Cuts at: 2503 2503
Size: 6358
SapI GCTCTTCn'nnn_
Cuts at: 2436 2436
Size: 6358
SbfI CC_TGCA'GG
Cuts at: 2793 3710 2793
Size: 917 5441
ScaI AGT'ACT
Cuts at: 4606 6318 4606
Size: 1712 4646
SfcI C'TryA_G
Cuts at: 826 1045 2436 2789 3326 3332 3706 4841 5522
Size: 219 1391 353 537 6 374 1135 681
Cuts at: 5522 5713 826
Size: 191 1471
Fragments arranged by size:
1471 1391 1135 681 537 374 353 219
191 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6358
SmaI CCC'GGG
Cuts at: 1872 3567 3685 1872
Size: 1695 118 4545
Fragments arranged by size:
4545 1695 118
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2585 4462 5363 5625 820
Size: 197 926 642 1877 901 262 1553
Fragments arranged by size:
1877 1553 926 901 642 262 197
SnaBI TAC'GTA
Cuts at: 493 4149 493
Size: 3656 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6358
SphI G_CATG'C
Cuts at: 1132 2101 3716 1132
Size: 969 1615 3774
Fragments arranged by size:
3774 1615 969
SspI AAT'ATT
Cuts at: 5 52 4282 5
Size: 47 4230 2081
Fragments arranged by size:
4230 2081 47
StuI AGG'CCT
Cuts at: 1517 2051 3131 1517
Size: 534 1080 4744
Fragments arranged by size:
4744 1080 534
StyI C'CwwG_G
Cuts at: 513 1065 2004 2238 2853 3099 3138 3198 3797
Size: 552 939 234 615 246 39 60 599
Cuts at: 3797 513
Size: 3074
Fragments arranged by size:
3074 939 615 599 552 246 234 60
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3305 4385 4402 4555 4740 5185 325
Size: 1255 1725 1080 17 153 185 445 1498
Fragments arranged by size:
1725 1498 1255 1080 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4604 6316 96
Size: 274 80 33 51 1443 2627 1712 138
Fragments arranged by size:
2627 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6318 6350 6318
Size: 32 6326
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6358
XbaI T'CTAG_A
Cuts at: 3694 3694
Size: 6358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3619 3619
Size: 6358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6358
XmaI C'CCGG_G
Cuts at: 1870 3565 3683 1870
Size: 1695 118 4545
Fragments arranged by size:
4545 1695 118
XmnI GAAnn'nnTTC
Cuts at: 4487 4487
Size: 6358
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4166 276
Size: 53 83 186 936 2632 2468
Fragments arranged by size:
2632 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI SexAI SfiI SfoI SrfI SwaI