Restriction map for
pFN21AB7854
(Circular) (Six-base) MAPSORT of: pFN21AB7854.seq Check: 6419 from: 1 to: 6580
pFN21AB7854 6580 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4390 278
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3923 1937
Size: 1986 4594
Acc65I G'GTAC_C
Cuts at: 3901 3901
Size: 6580
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2505 2682 2899 4643 6027 6295 1632
Size: 873 177 217 1744 1384 268 1917
Fragments arranged by size:
1917 1744 1384 873 268 217 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6383
AflIII A'CryG_T
Cuts at: 1203 2718 5479 6456 1203
Size: 1515 2761 977 1327
Fragments arranged by size:
2761 1515 1327 977
AgeI A'CCGG_T
Cuts at: 5411 5411
Size: 6580
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 2474 1166
Size: 1308 5272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6580
AlwNI CAG_nnn'CTG
Cuts at: 4323 5895 4323
Size: 1572 5008
ApaI G_GGCC'C
Cuts at: 2801 2801
Size: 6580
ApaLI G'TGCA_C
Cuts at: 2519 4637 5793 2519
Size: 2118 1156 3306
Fragments arranged by size:
3306 2118 1156
ApoI r'AATT_y
Cuts at: 1493 2401 2684 3889 4115 4267 6376 1493
Size: 908 283 1205 226 152 2109 1697
Fragments arranged by size:
2109 1697 1205 908 283 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2166 5134 160
Size: 872 1134 2968 1606
Fragments arranged by size:
2968 1606 1134 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 2226 2435 2529 3905 1870
Size: 73 283 209 94 1376 4545
Fragments arranged by size:
4545 1376 283 209 94 73
BamHI G'GATC_C
Cuts at: 3910 4277 3910
Size: 367 6213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 3901 5356 618
Size: 325 217 627 513 1601 1455 1842
Fragments arranged by size:
1842 1601 1455 627 513 325 217
BanII G_rGCy'C
Cuts at: 1391 2444 2801 3899 1391
Size: 1053 357 1098 4072
Fragments arranged by size:
4072 1098 1053 357
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2280 2905 2928 3241 928
Size: 971 381 625 23 313 4267
Fragments arranged by size:
4267 971 625 381 313 23
BbvCI CC'TCA_GC
Cuts at: 2709 2709
Size: 6580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2426 2460 4771 4805 1903
Size: 34 489 34 2311 34 3678
Fragments arranged by size:
3678 2311 489 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4472 5682 4472
Size: 1210 5370
BclI T'GATC_A
Cuts at: 1548 2725 1548
Size: 1177 5403
BfrBI ATG'CAT
Cuts at: 3659 3659
Size: 6580
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 5191 136
Size: 107 122 71 631 605 478 3041 1525
Fragments arranged by size:
3041 1525 631 605 478 122 107 71
BglII A'GATC_T
Cuts at: 6575 6575
Size: 6580
BlpI GC'TnA_GC
Cuts at: 3997 3997
Size: 6580
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2523 2589 2801 4641 5797 1790
Size: 262 471 66 212 1840 1156 2573
Fragments arranged by size:
2573 1840 1156 471 262 212 66
BmgBI CAC'GTC
Cuts at: 1318 2584 3085 1318
Size: 1266 501 4813
Fragments arranged by size:
4813 1266 501
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2052 4311 5269
Size: 416 53 83 399 24 635 2259 958
Cuts at: 5269 442
Size: 1753
Fragments arranged by size:
2259 1753 958 635 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3823 3823
Size: 6580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 5240 1239
Size: 1178 2823 2579
Fragments arranged by size:
2823 2579 1178
Bpu10I CC'TnA_GC
Cuts at: 2709 2709
Size: 6580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3145 4705 5570 5868 3145
Size: 1560 865 298 3857
Fragments arranged by size:
3857 1560 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2843 5243 882
Size: 617 1344 2400 2219
Fragments arranged by size:
2400 2219 1344 617
BsaAI yAC'GTr
Cuts at: 493 1204 3628 4371 493
Size: 711 2424 743 2702
Fragments arranged by size:
2702 2424 743 711
BsaBI GATnn'nnATC
Cuts at: 3107 6574 3107
Size: 3467 3113
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4387 4769 5404 275
Size: 53 83 186 936 2854 382 635 1451
Fragments arranged by size:
2854 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2638 2852 3482 5013 5411 5685 5832 6243 2638
Size: 214 630 1531 398 274 147 411 2975
Fragments arranged by size:
2975 1531 630 411 398 274 214 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2705 2735 1178
Size: 30 165 30 1302 30 5023
Fragments arranged by size:
5023 1302 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2346 2726 3130 3340 3578 1186
Size: 1160 380 404 210 238 4188
Fragments arranged by size:
4188 1160 404 380 238 210
BseYI C'CCAG_C
Cuts at: 5783 6279 5783
Size: 496 6084
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6007
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4030 4791 5819 1540
Size: 275 186 2029 761 1028 2301
Fragments arranged by size:
2301 2029 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2444 2523 3131 3621 3804 4641 4726 5797 2444
Size: 79 608 490 183 837 85 1071 3227
Fragments arranged by size:
3227 1071 837 608 490 183 85 79
BsmI GAATG_Cn'
Cuts at: 4091 4184 4091
Size: 93 6487
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2444 2523 2589 2801 3131 3621
Size: 399 262 392 79 66 212 330 490
Cuts at: 3621 3804 3899 4641 4726 5797 1391
Size: 183 95 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 490 399 392 330 262
212 183 95 85 79 66
BspEI T'CCGG_A
Cuts at: 2638 6243 2638
Size: 3605 2975
BspHI T'CATG_A
Cuts at: 4469 6199 4469
Size: 1730 4850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3480 3935 844
Size: 2636 455 3489
Fragments arranged by size:
3489 2636 455
BsrBI CCG'CTC
Cuts at: 1394 1823 3292 4467 1394
Size: 429 1469 1175 3507
Fragments arranged by size:
3507 1469 1175 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3003 5075 5249 65
Size: 1183 1755 2072 174 1396
Fragments arranged by size:
2072 1755 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5224 5411 1700
Size: 110 3414 187 2869
Fragments arranged by size:
3414 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6580
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6580
BssSI C'ACGA_G
Cuts at: 4640 5652 4640
Size: 1012 5568
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3062 794
Size: 2268 4312
BstBI TT'CG_AA
Cuts at: 5384 5384
Size: 6580
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6580
BstYI r'GATC_y
Cuts at: 1972 3910 4277 4662 4679 6131 6217 6229 6575
Size: 1938 367 385 17 1452 86 12 346
Cuts at: 6575 1972
Size: 1977
Fragments arranged by size:
1977 1938 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3555 3726 513
Size: 552 2490 171 3367
Fragments arranged by size:
3367 2490 552 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5841
Fragments arranged by size:
5841 683 56
BtsI GCAGTG_nn'
Cuts at: 3532 4096 4890 4910 3532
Size: 564 794 20 5202
Fragments arranged by size:
5202 794 564 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6545
DraI TTT'AAA
Cuts at: 3884 3884
Size: 6580
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5587 809
Size: 507 4271 1802
Fragments arranged by size:
4271 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2595 4027 4916
Size: 54 1425 50 275 18 765 1432 889
Cuts at: 4916 6545 8
Size: 1629 43
Fragments arranged by size:
1629 1432 1425 889 765 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4027 1537
Size: 275 2215 4090
Fragments arranged by size:
4090 2215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2077 2538 3042 3087 3282 3383 3449 3555
Size: 724 461 504 45 195 101 66 106
Cuts at: 3555 4510 1353
Size: 955 3423
Fragments arranged by size:
3423 955 724 504 461 195 106 101
66 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5167 5541 5687 387
Size: 1064 138 3578 374 146 1280
Fragments arranged by size:
3578 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2442 2442
Size: 6580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2505 2682 2899 4643 5240 6027
Size: 393 785 88 177 217 1744 597 787
Cuts at: 6027 6295 1239
Size: 268 1524
Fragments arranged by size:
1744 1524 787 785 597 393 268 217
177 88
EcoO109I rG'GnC_Cy
Cuts at: 2797 2798 2995 3688 2797
Size: 1 197 693 5689
Fragments arranged by size:
5689 693 197 1
EcoRI G'AATT_C
Cuts at: 2401 3889 4267 2401
Size: 1488 378 4714
Fragments arranged by size:
4714 1488 378
EcoRV GAT'ATC
Cuts at: 3705 3705
Size: 6580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 3040 2137
Size: 903 5677
FspI TGC'GCA
Cuts at: 5086 5086
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 2610 5727 1678
Size: 932 3117 2531
Fragments arranged by size:
3117 2531 932
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2334 2366 2544 2573 2576
Size: 32 135 32 762 32 178 29 3
Cuts at: 2576 2605 2717 2749 2865 2897 3125 3157 3413
Size: 29 112 32 116 32 228 32 256
Cuts at: 3413 3445 5221 5253 5295 5327 1373
Size: 32 1776 32 42 32 2626
Fragments arranged by size:
2626 1776 762 256 228 178 135 116
112 42 32 32 32 32 32 32
32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3924 4170 669
Size: 988 281 1986 246 3079
Fragments arranged by size:
3079 1986 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3743 748
Size: 2995 3585
HpaI GTT'AAC
Cuts at: 4170 4170
Size: 6580
KpnI G_GTAC'C
Cuts at: 3905 3905
Size: 6580
MfeI C'AATT_G
Cuts at: 4179 4179
Size: 6580
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1998 2896 5694 5878 1305
Size: 693 898 2798 184 2007
Fragments arranged by size:
2798 2007 898 693 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2597 10
Size: 54 1425 343 765 3993
Fragments arranged by size:
3993 1425 765 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 2474 3113 3773 3869 4538 4897
Size: 648 1290 18 639 660 96 669 359
Cuts at: 4897 5056 518
Size: 159 2042
Fragments arranged by size:
2042 1290 669 660 648 639 359 159
96 18
MspA1I CmG'CkG
Cuts at: 1606 3996 4673 5821 6066 1606
Size: 2390 677 1148 245 2120
Fragments arranged by size:
2390 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3555 513
Size: 552 2490 3538
Fragments arranged by size:
3538 2490 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6580
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NsiI A_TGCA'T
Cuts at: 3661 3661
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 2722 3938 5483 1132
Size: 97 1493 1216 1545 2229
Fragments arranged by size:
2229 1545 1493 1216 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6580
PciI A'CATG_T
Cuts at: 2718 5479 2718
Size: 2761 3819
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6580
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 6162 6194 4649
Size: 32 1481 32 5035
Fragments arranged by size:
5035 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2995 2995
Size: 6580
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6580
PsiI TTA'TAA
Cuts at: 3198 4150 3198
Size: 952 5628
PspOMI G'GGCC_C
Cuts at: 2797 2797
Size: 6580
PstI C_TGCA'G
Cuts at: 830 3477 3932 830
Size: 2647 455 3478
Fragments arranged by size:
3478 2647 455
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6580
SacI G_AGCT'C
Cuts at: 2444 2444
Size: 6580
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SapI GCTCTTCn'nnn_
Cuts at: 2077 2538 3282 2077
Size: 461 744 5375
Fragments arranged by size:
5375 744 461
SbfI CC_TGCA'GG
Cuts at: 3932 3932
Size: 6580
ScaI AGT'ACT
Cuts at: 4828 6540 4828
Size: 1712 4868
SfcI C'TryA_G
Cuts at: 826 1045 3473 3928 5063 5744 5935 826
Size: 219 2428 455 1135 681 191 1471
Fragments arranged by size:
2428 1471 1135 681 455 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 2531 3907 1872
Size: 659 1376 4545
Fragments arranged by size:
4545 1376 659
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2226 3124 4684 5585 5847 820
Size: 197 926 283 898 1560 901 262 1553
Fragments arranged by size:
1560 1553 926 901 898 283 262 197
SnaBI TAC'GTA
Cuts at: 493 4371 493
Size: 3878 2702
SpeI A'CTAG_T
Cuts at: 152 2035 152
Size: 1883 4697
SphI G_CATG'C
Cuts at: 1132 3938 1132
Size: 2806 3774
SspI AAT'ATT
Cuts at: 5 52 4504 5
Size: 47 4452 2081
Fragments arranged by size:
4452 2081 47
StuI AGG'CCT
Cuts at: 1517 3326 1517
Size: 1809 4771
StyI C'CwwG_G
Cuts at: 513 1065 2148 2296 3555 4019 513
Size: 552 1083 148 1259 464 3074
Fragments arranged by size:
3074 1259 1083 552 464 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 4607 4624 4777 4962 5407 325
Size: 1255 1035 1992 17 153 185 445 1498
Fragments arranged by size:
1992 1498 1255 1035 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2418 2715 2751
Size: 274 80 33 51 1443 441 297 36
Cuts at: 2751 4826 6538 96
Size: 2075 1712 138
Fragments arranged by size:
2075 1712 1443 441 297 274 138 80
51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2457 2489 6540 6572 2457
Size: 32 4051 32 2465
Fragments arranged by size:
4051 2465 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6580
XbaI T'CTAG_A
Cuts at: 3916 3916
Size: 6580
XhoI C'TCGA_G
Cuts at: 1943 2226 1943
Size: 283 6297
XmaI C'CCGG_G
Cuts at: 1870 2529 3905 1870
Size: 659 1376 4545
Fragments arranged by size:
4545 1376 659
XmnI GAAnn'nnTTC
Cuts at: 3104 4709 3104
Size: 1605 4975
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4388 276
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI