Restriction map for
pFN21AB7851
(Circular) (Six-base) MAPSORT of: pFN21AB7851.seq Check: 9871 from: 1 to: 6349
pFN21AB7851 6349 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3394 3394
Size: 6349
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2967 3692 1937
Size: 1030 725 4594
Fragments arranged by size:
4594 1030 725
Acc65I G'GTAC_C
Cuts at: 2940 3670 2940
Size: 730 5619
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2902 4412 5796 6064 1632
Size: 1270 1510 1384 268 1917
Fragments arranged by size:
1917 1510 1384 1270 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 5248 6225 1203
Size: 4045 977 1327
Fragments arranged by size:
4045 1327 977
AgeI A'CCGG_T
Cuts at: 3079 5180 3079
Size: 2101 4248
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3448 1184
Size: 2264 4085
AlwNI CAG_nnn'CTG
Cuts at: 2568 4092 5664 2568
Size: 1524 1572 3253
Fragments arranged by size:
3253 1572 1524
ApaI G_GGCC'C
Cuts at: 2257 2664 2886 3503 3576 2257
Size: 407 222 617 73 5030
Fragments arranged by size:
5030 617 407 222 73
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 2165 3658 3884 4036 6145 1493
Size: 672 1493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 672 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2718 3068 3419 3674 1870
Size: 73 775 350 351 255 4545
Fragments arranged by size:
4545 775 351 350 255 73
BamHI G'GATC_C
Cuts at: 2611 2774 3679 4046 2611
Size: 163 905 367 4914
Fragments arranged by size:
4914 905 367 163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2648 2940 3019 3064 3415
Size: 325 217 627 861 292 79 45 351
Cuts at: 3415 3670 5125 618
Size: 255 1455 1842
Fragments arranged by size:
1842 1455 861 627 351 325 292 255
217 79 45
BanII G_rGCy'C
Cuts at: 1391 2257 2664 2858 2886 3291 3408 3503 3576
Size: 866 407 194 28 405 117 95 73
Cuts at: 3576 3599 3668 1391
Size: 23 69 4072
Fragments arranged by size:
4072 866 407 405 194 117 95 73
69 28 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 2689 3357 3480 928
Size: 971 116 674 668 123 3797
Fragments arranged by size:
3797 971 674 668 123 116
BbvCI CC'TCA_GC
Cuts at: 2738 2790 2981 3234 2738
Size: 52 191 253 5853
Fragments arranged by size:
5853 253 191 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4540 4574 1903
Size: 34 2603 34 3678
Fragments arranged by size:
3678 2603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2547 4960 136
Size: 107 122 71 631 605 875 2413 1525
Fragments arranged by size:
2413 1525 875 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 2246 2852 3766 2246
Size: 606 914 4829
Fragments arranged by size:
4829 914 606
Bme1580I G_kGCm'C
Cuts at: 1790 2257 2391 2401 2664 2886 3024 3050 3382
Size: 467 134 10 263 222 138 26 332
Cuts at: 3382 3503 3576 4410 5566 1790
Size: 121 73 834 1156 2573
Fragments arranged by size:
2573 1156 834 467 332 263 222 138
134 121 73 26 10
BmgBI CAC'GTC
Cuts at: 1318 2583 1318
Size: 1265 5084
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2862 3424 4080
Size: 416 53 83 399 24 1445 562 656
Cuts at: 4080 5038 442
Size: 958 1753
Fragments arranged by size:
1753 1445 958 656 562 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3254 1056
Size: 2198 4151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3310 3310
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2612 3125 3487 3590 3653 5009 1239
Size: 887 486 513 362 103 63 1356 2579
Fragments arranged by size:
2579 1356 887 513 486 362 103 63
Bpu10I CC'TnA_GC
Cuts at: 2738 2790 2893 2981 3234 3396 3402 2738
Size: 52 103 88 253 162 6 5685
Fragments arranged by size:
5685 253 162 103 88 52 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2059 3428 4474 5339 5637 2059
Size: 1369 1046 865 298 2771
Fragments arranged by size:
2771 1369 1046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3065 5012 882
Size: 617 1566 1947 2219
Fragments arranged by size:
2219 1947 1566 617
BsaAI yAC'GTr
Cuts at: 493 1204 4140 493
Size: 711 2936 2702
Fragments arranged by size:
2936 2702 711
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 2816 3160 3371
Size: 53 83 186 936 603 680 344 211
Cuts at: 3371 4156 4538 5173 275
Size: 785 382 635 1451
Fragments arranged by size:
1451 936 785 680 635 603 382 344
211 186 83 53
BsaWI w'CCGG_w
Cuts at: 2638 3079 4782 5180 5454 5601 6012 2638
Size: 441 1703 398 274 147 411 2975
Fragments arranged by size:
2975 1703 441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3629 3659 1178
Size: 30 165 30 2226 30 3868
Fragments arranged by size:
3868 2226 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 3079 3326 1186
Size: 952 941 247 4209
Fragments arranged by size:
4209 952 941 247
BseYI C'CCAG_C
Cuts at: 2375 2602 2885 3024 3030 3176 3506 3566 3621
Size: 227 283 139 6 146 330 60 55
Cuts at: 3621 5552 6048 2375
Size: 1931 496 2676
Fragments arranged by size:
2676 1931 496 330 283 227 146 139
60 55 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2270 2403 2984 3240 3372 1163
Size: 573 534 133 581 256 132 4140
Fragments arranged by size:
4140 581 573 534 256 133 132
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3799 4560 5588 1540
Size: 275 186 1798 761 1028 2301
Fragments arranged by size:
2301 1798 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3599 3639 4410 4495 5566 3599
Size: 40 771 85 1071 4382
Fragments arranged by size:
4382 1071 771 85 40
BsmI GAATG_Cn'
Cuts at: 2280 3860 3953 2280
Size: 1580 93 4676
Fragments arranged by size:
4676 1580 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2257 2391 2401 2664 2858 2886 3024
Size: 399 467 134 10 263 194 28 138
Cuts at: 3024 3050 3291 3382 3408 3503 3576 3599 3639
Size: 26 241 91 26 95 73 23 40
Cuts at: 3639 3668 4410 4495 5566 1391
Size: 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 467 399 263 241 194
138 134 95 91 85 73 40 29
28 26 26 23 10
BspEI T'CCGG_A
Cuts at: 2638 6012 2638
Size: 3374 2975
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2952 3394 3704 844
Size: 2108 442 310 3489
Fragments arranged by size:
3489 2108 442 310
BsrBI CCG'CTC
Cuts at: 1394 1823 2484 3210 4236 1394
Size: 429 661 726 1026 3507
Fragments arranged by size:
3507 1026 726 661 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2139 4844 5018 65
Size: 1183 891 2705 174 1396
Fragments arranged by size:
2705 1396 1183 891 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2765 3079 3162 3194 4993 5180 1700
Size: 110 955 314 83 32 1799 187 2869
Fragments arranged by size:
2869 1799 955 314 187 110 83 32
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6349
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstXI CCAn_nnnn'nTGG
Cuts at: 2690 2827 2690
Size: 137 6212
BstYI r'GATC_y
Cuts at: 1972 2082 2611 2641 2774 3679 4046 4431 4448
Size: 110 529 30 133 905 367 385 17
Cuts at: 4448 5900 5986 5998 6344 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 905 529 385 367 346 133
110 86 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 2925 2925
Size: 6349
BtgI C'CryG_G
Cuts at: 513 1065 3060 3615 513
Size: 552 1995 555 3247
Fragments arranged by size:
3247 1995 555 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5610
Fragments arranged by size:
5610 683 56
BtsI GCAGTG_nn'
Cuts at: 2391 3223 3865 4659 4679 2391
Size: 832 642 794 20 4061
Fragments arranged by size:
4061 832 794 642 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3193 3228 529
Size: 35 2629 35 3650
Fragments arranged by size:
3650 2629 35 35
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2693 5356 809
Size: 507 1377 2663 1802
Fragments arranged by size:
2663 1802 1377 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2763 3796 4685
Size: 54 1425 50 275 18 933 1033 889
Cuts at: 4685 6314 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1033 933 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3796 1537
Size: 275 1984 4090
Fragments arranged by size:
4090 1984 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2506 2525 3112 4279 1353
Size: 1153 19 587 1167 3423
Fragments arranged by size:
3423 1167 1153 587 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 4936 5310 5456 387
Size: 1064 138 444 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 444 374 146 138
EcoICRI GAG'CTC
Cuts at: 3597 3597
Size: 6349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2612 2902 3125 3487 3590 3653
Size: 393 494 486 290 223 362 103 63
Cuts at: 3653 4412 5009 5796 6064 1239
Size: 759 597 787 268 1524
Fragments arranged by size:
1524 787 759 597 494 486 393 362
290 268 223 103 63
EcoO109I rG'GnC_Cy
Cuts at: 2254 2476 2660 2661 2882 3499 3500 3554 3572
Size: 222 184 1 221 617 1 54 18
Cuts at: 3572 3573 2254
Size: 1 5030
Fragments arranged by size:
5030 617 222 221 184 54 18 1
1 1
EcoRI G'AATT_C
Cuts at: 2165 3658 4036 2165
Size: 1493 378 4478
Fragments arranged by size:
4478 1493 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3469 3469
Size: 6349
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 3037 3251 3466 5496 1678
Size: 1359 214 215 2030 2531
Fragments arranged by size:
2531 2030 1359 215 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2447 2468 2479 2500 2544
Size: 32 135 32 875 21 11 21 44
Cuts at: 2544 2567 2576 2599 4990 5022 5064 5096 1373
Size: 23 9 23 2391 32 42 32 2626
Fragments arranged by size:
2626 2391 875 135 44 42 32 32
32 32 23 23 21 21 11 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 2944 3674 2944
Size: 730 5619
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3288 5463 5647 1305
Size: 1983 2175 184 2007
Fragments arranged by size:
2175 2007 1983 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4527
Fragments arranged by size:
4527 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 3430 4307 4666 4825 518
Size: 648 1197 1067 877 359 159 2042
Fragments arranged by size:
2042 1197 1067 877 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2602 2668 2889 3052 3169 3176 3506
Size: 483 513 66 221 163 117 7 330
Cuts at: 3506 3765 4442 5590 5835 1606
Size: 259 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 513 483 330 259 245
221 163 117 66 7
NaeI GCC'GGC
Cuts at: 3164 3164
Size: 6349
NcoI C'CATG_G
Cuts at: 513 1065 3060 513
Size: 552 1995 3802
Fragments arranged by size:
3802 1995 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NgoMIV G'CCGG_C
Cuts at: 3162 3162
Size: 6349
NheI G'CTAG_C
Cuts at: 1052 3250 1052
Size: 2198 4151
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 3439 3614 3707 5252 1132
Size: 97 2210 175 93 1545 2229
Fragments arranged by size:
2229 2210 1545 175 97 93
PasI CC'CwG_GG
Cuts at: 1348 3008 1348
Size: 1660 4689
PciI A'CATG_T
Cuts at: 5248 5248
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6349
PfoI T'CCnGG_A
Cuts at: 2037 2103 2770 2037
Size: 66 667 5616
Fragments arranged by size:
5616 667 66
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2476 2476
Size: 6349
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PspOMI G'GGCC_C
Cuts at: 2253 2660 2882 3499 3572 2253
Size: 407 222 617 73 5030
Fragments arranged by size:
5030 617 407 222 73
PstI C_TGCA'G
Cuts at: 830 2949 3701 830
Size: 2119 752 3478
Fragments arranged by size:
3478 2119 752
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 2089 3176 2089
Size: 1087 5262
SacI G_AGCT'C
Cuts at: 3599 3599
Size: 6349
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SapI GCTCTTCn'nnn_
Cuts at: 2506 2506
Size: 6349
SbfI CC_TGCA'GG
Cuts at: 2949 3701 2949
Size: 752 5597
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SfcI C'TryA_G
Cuts at: 826 1045 2569 2945 3697 4832 5513 5704 826
Size: 219 1524 376 752 1135 681 191 1471
Fragments arranged by size:
1524 1471 1135 752 681 376 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 3421 3676 1872
Size: 1549 255 4545
Fragments arranged by size:
4545 1549 255
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2074 3443 4453 5354 5616 820
Size: 197 926 131 1369 1010 901 262 1553
Fragments arranged by size:
1553 1369 1010 926 901 262 197 131
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3439 3614 3707 1132
Size: 2307 175 93 3774
Fragments arranged by size:
3774 2307 175 93
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6349
StyI C'CwwG_G
Cuts at: 513 1065 2295 3060 3584 3788 513
Size: 552 1230 765 524 204 3074
Fragments arranged by size:
3074 1230 765 552 524 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2837 2972 4376 4393 4546 4731 5176
Size: 1255 1257 135 1404 17 153 185 445
Cuts at: 5176 325
Size: 1498
Fragments arranged by size:
1498 1404 1257 1255 445 185 153 135
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4595 6307 96
Size: 274 80 33 51 1443 2618 1712 138
Fragments arranged by size:
2618 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 6309 6341 3629
Size: 32 2648 32 3637
Fragments arranged by size:
3637 2648 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 3419 3674 1870
Size: 1549 255 4545
Fragments arranged by size:
4545 1549 255
XmnI GAAnn'nnTTC
Cuts at: 2306 4478 2306
Size: 2172 4177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI