Restriction map for
pFN21AB7849
(Circular) (Six-base) MAPSORT of: pFN21AB7849.seq Check: 4692 from: 1 to: 6340
pFN21AB7849 6340 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3683 1937
Size: 1746 4594
Acc65I G'GTAC_C
Cuts at: 3090 3661 3090
Size: 571 5769
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2818 3070 3322 3406 3574 4403 5787 6055
Size: 1186 252 252 84 168 829 1384 268
Cuts at: 6055 1632
Size: 1917
Fragments arranged by size:
1917 1384 1186 829 268 252 252 168
84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 2692 2860 3196 3616 5239 6216 1203
Size: 1489 168 336 420 1623 977 1327
Fragments arranged by size:
1623 1489 1327 977 420 336 168
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 5069 5069
Size: 6340
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6340
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 2111
Size: 32 6308
AlwNI CAG_nnn'CTG
Cuts at: 2600 4083 5655 2600
Size: 1483 1572 3285
Fragments arranged by size:
3285 1572 1483
ApaLI G'TGCA_C
Cuts at: 2396 2434 3530 4397 5553 2396
Size: 38 1096 867 1156 3183
Fragments arranged by size:
3183 1156 1096 867 38
ApoI r'AATT_y
Cuts at: 1493 3649 3875 4027 6136 1493
Size: 2156 226 152 2109 1697
Fragments arranged by size:
2156 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 3665 1870
Size: 73 1722 4545
Fragments arranged by size:
4545 1722 73
BamHI G'GATC_C
Cuts at: 3670 4037 3670
Size: 367 5973
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3090 3661 5116 618
Size: 325 217 627 1303 571 1455 1842
Fragments arranged by size:
1842 1455 1303 627 571 325 217
BanII G_rGCy'C
Cuts at: 1391 3659 1391
Size: 2268 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2750 3199 928
Size: 971 851 449 4069
Fragments arranged by size:
4069 971 851 449
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2437 2471 4531 4565 1903
Size: 34 500 34 2060 34 3678
Fragments arranged by size:
3678 2060 500 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4232 5442 4232
Size: 1210 5130
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6340
BfrBI ATG'CAT
Cuts at: 2726 2726
Size: 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4951 136
Size: 107 122 71 631 605 3279 1525
Fragments arranged by size:
3279 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2471 6335 2471
Size: 3864 2476
BlpI GC'TnA_GC
Cuts at: 3757 3757
Size: 6340
Bme1580I G_kGCm'C
Cuts at: 1790 2400 2438 3534 4401 5557 1790
Size: 610 38 1096 867 1156 2573
Fragments arranged by size:
2573 1156 1096 867 610 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6340
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4071 5029 442
Size: 416 53 83 399 24 2654 958 1753
Fragments arranged by size:
2654 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2301 2898 2982 3318 3570 3654 5000
Size: 887 175 597 84 336 252 84 1346
Cuts at: 5000 1239
Size: 2579
Fragments arranged by size:
2579 1346 887 597 336 252 175 84
84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2392 2572 4465 5330 5628 2392
Size: 180 1893 865 298 3104
Fragments arranged by size:
3104 1893 865 298 180
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2102 2360 2482 3550 5003 882
Size: 617 603 258 122 1068 1453 2219
Fragments arranged by size:
2219 1453 1068 617 603 258 122
BsaAI yAC'GTr
Cuts at: 493 1204 2611 2779 2947 3283 3535 4131 493
Size: 711 1407 168 168 336 252 596 2702
Fragments arranged by size:
2702 1407 711 596 336 252 168 168
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4147 4529 5164 275
Size: 53 83 186 936 2614 382 635 1451
Fragments arranged by size:
2614 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3128 4773 5171 5445 5592 6003 3128
Size: 1645 398 274 147 411 3465
Fragments arranged by size:
3465 1645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 2320 2350 1178
Size: 30 165 30 708 30 179 30 5168
Fragments arranged by size:
5168 708 179 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2095 2920 3088 3256 3340 1186
Size: 909 825 168 168 84 4186
Fragments arranged by size:
4186 909 825 168 168 84
BseYI C'CCAG_C
Cuts at: 2323 5543 6039 2323
Size: 3220 496 2624
Fragments arranged by size:
3220 2624 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5767
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3790 4551 5579 1540
Size: 275 186 1789 761 1028 2301
Fragments arranged by size:
2301 1789 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2400 2438 3112 3534 4401 4486 5557 2400
Size: 38 674 422 867 85 1071 3183
Fragments arranged by size:
3183 1071 867 674 422 85 38
BsiWI C'GTAC_G
Cuts at: 3393 3393
Size: 6340
BsmI GAATG_Cn'
Cuts at: 3077 3126 3572 3851 3944 3077
Size: 49 446 279 93 5473
Fragments arranged by size:
5473 446 279 93 49
BsmBI CGTCTCn'nnnn_
Cuts at: 3021 3021
Size: 6340
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2400 2438 3112 3534 3659 4401 4486
Size: 399 610 38 674 422 125 742 85
Cuts at: 4486 5557 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 674 610 422 399 125
85 38
BspEI T'CCGG_A
Cuts at: 6003 6003
Size: 6340
BspHI T'CATG_A
Cuts at: 4229 5959 4229
Size: 1730 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2120 3695 844
Size: 1276 1575 3489
Fragments arranged by size:
3489 1575 1276
BsrBI CCG'CTC
Cuts at: 1394 1823 3638 4227 1394
Size: 429 1815 589 3507
Fragments arranged by size:
3507 1815 589 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2060 2986 4835 5009 65
Size: 1183 812 926 1849 174 1396
Fragments arranged by size:
1849 1396 1183 926 812 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4984 5171 1700
Size: 110 3174 187 2869
Fragments arranged by size:
3174 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 4400 5412 4400
Size: 1012 5328
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6340
BstBI TT'CG_AA
Cuts at: 3274 5144 3274
Size: 1870 4470
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6340
BstXI CCAn_nnnn'nTGG
Cuts at: 2055 2055
Size: 6340
BstYI r'GATC_y
Cuts at: 1972 2471 3670 4037 4422 4439 5891 5977 5989
Size: 499 1199 367 385 17 1452 86 12
Cuts at: 5989 6335 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1199 499 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4849
Fragments arranged by size:
4849 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3588 487
Size: 683 56 2362 3239
Fragments arranged by size:
3239 2362 683 56
BtsI GCAGTG_nn'
Cuts at: 3161 3581 3856 4650 4670 3161
Size: 420 275 794 20 4831
Fragments arranged by size:
4831 794 420 275 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3139 3174 3559 3594 529
Size: 35 2575 35 385 35 3275
Fragments arranged by size:
3275 2575 385 35 35 35
DraI TTT'AAA
Cuts at: 3644 3644
Size: 6340
DraIII CAC_nnn'GTG
Cuts at: 2442 3464 2442
Size: 1022 5318
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5347 809
Size: 507 4031 1802
Fragments arranged by size:
4031 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3787 4676 6305
Size: 54 1425 50 275 18 1957 889 1629
Cuts at: 6305 8
Size: 43
Fragments arranged by size:
1957 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3787 1537
Size: 275 1975 4090
Fragments arranged by size:
4090 1975 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2046 3024 4270 1353
Size: 693 978 1246 3423
Fragments arranged by size:
3423 1246 978 693
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4927 5301 5447 387
Size: 1064 138 3338 374 146 1280
Fragments arranged by size:
3338 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2301 2818 2898 2982 3070 3318
Size: 393 494 175 517 80 84 88 248
Cuts at: 3318 3322 3406 3570 3574 3654 4403 5000 5787
Size: 4 84 164 4 80 749 597 787
Cuts at: 5787 6055 1239
Size: 268 1524
Fragments arranged by size:
1524 787 749 597 517 494 393 268
248 175 164 88 84 84 80 80
4 4
EcoRI G'AATT_C
Cuts at: 3649 4027 3649
Size: 378 5962
FspI TGC'GCA
Cuts at: 4846 4846
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 5487 1678
Size: 3809 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 2847 2879 4981
Size: 32 135 32 656 32 587 32 2102
Cuts at: 4981 5013 5055 5087 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2102 656 587 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3684 3930 669
Size: 988 281 1746 246 3079
Fragments arranged by size:
3079 1746 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2661 748
Size: 1913 4427
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KpnI G_GTAC'C
Cuts at: 3094 3665 3094
Size: 571 5769
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5454 5638 1305
Size: 4149 184 2007
Fragments arranged by size:
4149 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4518
Fragments arranged by size:
4518 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3119 4298 4657 4816 518
Size: 648 1953 1179 359 159 2042
Fragments arranged by size:
2042 1953 1179 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2323 2520 2838 2922 3213 3342 3381 3756
Size: 717 197 318 84 291 129 39 375
Cuts at: 3756 4433 5581 5826 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 717 677 375 318 291 245
197 129 84 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4849
Fragments arranged by size:
4849 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6340
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6340
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NsiI A_TGCA'T
Cuts at: 2728 2728
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 2696 2730 2864 3200 3368 3452 3620
Size: 97 1467 34 134 336 168 84 168
Cuts at: 3620 3632 3698 5243 1132
Size: 12 66 1545 2229
Fragments arranged by size:
2229 1545 1467 336 168 168 134 97
84 66 34 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6340
PciI A'CATG_T
Cuts at: 2692 2860 3196 3616 5239 2692
Size: 168 336 420 1623 3793
Fragments arranged by size:
3793 1623 420 336 168
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6340
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PmlI CAC'GTG
Cuts at: 2947 3283 3535 2947
Size: 336 252 5752
Fragments arranged by size:
5752 336 252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2143 4409 4441 5922 5954 2111
Size: 32 2266 32 1481 32 2497
Fragments arranged by size:
2497 2266 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6340
PsiI TTA'TAA
Cuts at: 3910 3910
Size: 6340
PstI C_TGCA'G
Cuts at: 830 2117 2519 3692 830
Size: 1287 402 1173 3478
Fragments arranged by size:
3478 1287 1173 402
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
PvuII CAG'CTG
Cuts at: 2323 2520 2838 2922 3342 2323
Size: 197 318 84 420 5321
Fragments arranged by size:
5321 420 318 197 84
SalI G'TCGA_C
Cuts at: 1936 3682 1936
Size: 1746 4594
SbfI CC_TGCA'GG
Cuts at: 2117 3692 2117
Size: 1575 4765
ScaI AGT'ACT
Cuts at: 3437 4588 6300 3437
Size: 1151 1712 3477
Fragments arranged by size:
3477 1712 1151
SfcI C'TryA_G
Cuts at: 826 1045 2089 2113 2515 2559 3253 3505 3688
Size: 219 1044 24 402 44 694 252 183
Cuts at: 3688 4823 5504 5695 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1044 694 681 402 252 219
191 183 44 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 3667 1872
Size: 1795 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2371 2587 4444 5345 5607 820
Size: 197 926 428 216 1857 901 262 1553
Fragments arranged by size:
1857 1553 926 901 428 262 216 197
SnaBI TAC'GTA
Cuts at: 493 4131 493
Size: 3638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 3698 1132
Size: 2566 3774
SspI AAT'ATT
Cuts at: 5 52 4264 5
Size: 47 4212 2081
Fragments arranged by size:
4212 2081 47
StuI AGG'CCT
Cuts at: 1517 3167 1517
Size: 1650 4690
StyI C'CwwG_G
Cuts at: 513 1065 2004 2130 3779 513
Size: 552 939 126 1649 3074
Fragments arranged by size:
3074 1649 939 552 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4367 4384 4537 4722 5167 325
Size: 1255 2787 17 153 185 445 1498
Fragments arranged by size:
2787 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3435 3485 4586
Size: 274 80 33 51 1443 1458 50 1101
Cuts at: 4586 6298 96
Size: 1712 138
Fragments arranged by size:
1712 1458 1443 1101 274 138 80 51
50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2320 2352 6300 6332 2320
Size: 32 3948 32 2328
Fragments arranged by size:
3948 2328 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6340
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6340
XmaI C'CCGG_G
Cuts at: 1870 3665 1870
Size: 1795 4545
XmnI GAAnn'nnTTC
Cuts at: 2236 2831 3167 3335 3419 3587 4469 2236
Size: 595 336 168 84 168 882 4107
Fragments arranged by size:
4107 882 595 336 168 168 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI