Restriction map for
pFN21AB7848
(Circular) (Six-base) MAPSORT of: pFN21AB7848.seq Check: 2525 from: 1 to: 6571
pFN21AB7848 6571 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2647 2647
Size: 6571
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3914 1937
Size: 1977 4594
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 4698 5071 1563
Size: 3135 373 3063
Fragments arranged by size:
3135 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3134 4634 6018 6286 1632
Size: 1502 1500 1384 268 1917
Fragments arranged by size:
1917 1502 1500 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 2121 5470 6447 1203
Size: 918 3349 977 1327
Fragments arranged by size:
3349 1327 977 918
AgeI A'CCGG_T
Cuts at: 5402 5402
Size: 6571
AhdI GACnn_n'nnGTC
Cuts at: 3074 5300 3074
Size: 2226 4345
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6571
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3590 1184
Size: 2406 4165
AlwNI CAG_nnn'CTG
Cuts at: 2903 3796 4314 5886 2903
Size: 893 518 1572 3588
Fragments arranged by size:
3588 1572 893 518
ApaI G_GGCC'C
Cuts at: 2218 2703 3552 3620 2218
Size: 485 849 68 5169
Fragments arranged by size:
5169 849 485 68
ApaLI G'TGCA_C
Cuts at: 4628 5784 4628
Size: 1156 5415
ApoI r'AATT_y
Cuts at: 1493 3880 4106 4258 6367 1493
Size: 2387 226 152 2109 1697
Fragments arranged by size:
2387 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 3410 3510 3620 3896 1870
Size: 73 100 1367 100 110 276 4545
Fragments arranged by size:
4545 1367 276 110 100 100 73
BamHI G'GATC_C
Cuts at: 3901 4268 3901
Size: 367 6204
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2425 2440 3168 3174 3374
Size: 325 217 627 638 15 728 6 200
Cuts at: 3374 3601 3629 3671 3729 3892 5347 618
Size: 227 28 42 58 163 1455 1842
Fragments arranged by size:
1842 1455 728 638 627 325 227 217
200 163 58 42 28 15 6
BanII G_rGCy'C
Cuts at: 1391 2218 2703 3141 3336 3509 3552 3620 3890
Size: 827 485 438 195 173 43 68 270
Cuts at: 3890 1391
Size: 4072
Fragments arranged by size:
4072 827 485 438 270 195 173 68
43
BbeI G_GCGC'C
Cuts at: 3178 3675 3733 3178
Size: 497 58 6016
Fragments arranged by size:
6016 497 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2448 928
Size: 971 549 5051
Fragments arranged by size:
5051 971 549
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4762 4796 1903
Size: 34 2825 34 3678
Fragments arranged by size:
3678 2825 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4463 5673 4463
Size: 1210 5361
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6571
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3159 3495 3513
Size: 107 122 71 631 605 1487 336 18
Cuts at: 3513 3763 5182 136
Size: 250 1419 1525
Fragments arranged by size:
1525 1487 1419 631 605 336 250 122
107 71 18
BglII A'GATC_T
Cuts at: 6566 6566
Size: 6571
BlpI GC'TnA_GC
Cuts at: 2087 2816 3988 2087
Size: 729 1172 4670
Fragments arranged by size:
4670 1172 729
Bme1580I G_kGCm'C
Cuts at: 1790 2218 2445 2578 2703 3552 3604 3620 4632
Size: 428 227 133 125 849 52 16 1012
Cuts at: 4632 5788 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1012 849 428 227 133 125
52 16
BmgBI CAC'GTC
Cuts at: 1318 2122 1318
Size: 804 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2439 4302 5260
Size: 416 53 83 399 24 1022 1863 958
Cuts at: 5260 442
Size: 1753
Fragments arranged by size:
1863 1753 1022 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3238 3238
Size: 6571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2528 2654 2765 5231 1239
Size: 863 426 126 111 2466 2579
Fragments arranged by size:
2579 2466 863 426 126 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2415 2462 4696 5561 5859 2415
Size: 47 2234 865 298 3127
Fragments arranged by size:
3127 2234 865 298 47
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5234 882
Size: 617 3735 2219
Fragments arranged by size:
3735 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3787 4362 493
Size: 711 2583 575 2702
Fragments arranged by size:
2702 2583 711 575
BsaBI GATnn'nnATC
Cuts at: 6565 6565
Size: 6571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3175 3672 3730 4378
Size: 53 83 186 936 1642 497 58 648
Cuts at: 4378 4760 5395 275
Size: 382 635 1451
Fragments arranged by size:
1642 1451 936 648 635 497 382 186
83 58 53
BsaWI w'CCGG_w
Cuts at: 5004 5402 5676 5823 6234 5004
Size: 398 274 147 411 5341
Fragments arranged by size:
5341 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 2922 2952 1178
Size: 30 165 30 825 30 664 30 4797
Fragments arranged by size:
4797 825 664 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6571
BseYI C'CCAG_C
Cuts at: 3242 3710 5774 6270 3242
Size: 468 2064 496 3543
Fragments arranged by size:
3543 2064 496 468
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2627 3255 3372 3809 1163
Size: 573 891 628 117 437 3925
Fragments arranged by size:
3925 891 628 573 437 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2912 3407 3811 4021 4782 5810
Size: 275 186 911 495 404 210 761 1028
Cuts at: 5810 1540
Size: 2301
Fragments arranged by size:
2301 1028 911 761 495 404 275 210
186
BsiHKAI G_wGCw'C
Cuts at: 2075 3336 4632 4717 5788 2075
Size: 1261 1296 85 1071 2858
Fragments arranged by size:
2858 1296 1261 1071 85
BsiWI C'GTAC_G
Cuts at: 3759 3759
Size: 6571
BsmI GAATG_Cn'
Cuts at: 4082 4175 4082
Size: 93 6478
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2218 2445 2578 2703 3141 3336
Size: 399 285 143 227 133 125 438 195
Cuts at: 3336 3509 3552 3604 3620 3890 4632 4717 5788
Size: 173 43 52 16 270 742 85 1071
Cuts at: 5788 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 438 399 285 270 227
195 173 143 133 125 85 52 43
16
BspEI T'CCGG_A
Cuts at: 6234 6234
Size: 6571
BspHI T'CATG_A
Cuts at: 2166 4460 6190 2166
Size: 2294 1730 2547
Fragments arranged by size:
2547 2294 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2647 3195 3926 844
Size: 1803 548 731 3489
Fragments arranged by size:
3489 1803 731 548
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 2914 3868 4458 1394
Size: 429 200 891 954 590 3507
Fragments arranged by size:
3507 954 891 590 429 200
BsrDI GCAATG_nn'
Cuts at: 65 1248 5066 5240 65
Size: 1183 3818 174 1396
Fragments arranged by size:
3818 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2646 3171 3377 3427 3626 3831 5215
Size: 110 836 525 206 50 199 205 1384
Cuts at: 5215 5402 1700
Size: 187 2869
Fragments arranged by size:
2869 1384 836 525 206 205 199 187
110 50
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6571
BssHII G'CGCG_C
Cuts at: 1925 3593 1925
Size: 1668 4903
BssSI C'ACGA_G
Cuts at: 2499 4631 5643 2499
Size: 2132 1012 3427
Fragments arranged by size:
3427 2132 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2650 2872 3022 3168 3796 794
Size: 1856 222 150 146 628 3569
Fragments arranged by size:
3569 1856 628 222 150 146
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 3307 1184
Size: 2123 4448
BstXI CCAn_nnnn'nTGG
Cuts at: 3094 3094
Size: 6571
BstYI r'GATC_y
Cuts at: 1972 2037 2889 2899 3901 4268 4653 4670 6122
Size: 65 852 10 1002 367 385 17 1452
Cuts at: 6122 6208 6220 6566 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1002 852 385 367 346 86
65 17 12 10
Bsu36I CC'TnA_GG
Cuts at: 2310 2310
Size: 6571
BtgI C'CryG_G
Cuts at: 513 1065 2934 3191 3393 3401 3724 513
Size: 552 1869 257 202 8 323 3360
Fragments arranged by size:
3360 1869 552 323 257 202 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2106 487
Size: 683 56 880 4952
Fragments arranged by size:
4952 880 683 56
BtsI GCAGTG_nn'
Cuts at: 2267 2731 4087 4881 4901 2267
Size: 464 1356 794 20 3937
Fragments arranged by size:
3937 1356 794 464 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3130 3165 3352 3387 529
Size: 35 2566 35 187 35 3713
Fragments arranged by size:
3713 2566 187 35 35 35
DraI TTT'AAA
Cuts at: 3875 3875
Size: 6571
DraIII CAC_nnn'GTG
Cuts at: 2494 2494
Size: 6571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5578 809
Size: 507 4262 1802
Fragments arranged by size:
4262 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2909 3315 3360
Size: 54 1425 50 275 18 1079 406 45
Cuts at: 3360 3379 3404 3808 4018 4907 6536 8
Size: 19 25 404 210 889 1629 43
Fragments arranged by size:
1629 1425 1079 889 406 404 275 210
54 50 45 43 25 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2909 3404 3808 4018 1537
Size: 275 1097 495 404 210 4090
Fragments arranged by size:
4090 1097 495 404 275 210
EarI CTCTTCn'nnn_
Cuts at: 1353 2013 3644 4501 1353
Size: 660 1631 857 3423
Fragments arranged by size:
3423 1631 857 660
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2081 3537 5158 5532 5678 387
Size: 1064 138 492 1456 1621 374 146 1280
Fragments arranged by size:
1621 1456 1280 1064 492 374 146 138
EcoICRI GAG'CTC
Cuts at: 3334 3334
Size: 6571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2528 2654 2765 3134 4634 5231
Size: 393 470 426 126 111 369 1500 597
Cuts at: 5231 6018 6286 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1500 787 597 470 426 393 369
268 126 111
EcoO109I rG'GnC_Cy
Cuts at: 2214 2215 2699 2700 2842 3292 3570 3616 3617
Size: 1 484 1 142 450 278 46 1
Cuts at: 3617 2214
Size: 5168
Fragments arranged by size:
5168 484 450 278 142 46 1 1
1
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
FspI TGC'GCA
Cuts at: 5077 5077
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 2108 3178 3480 3675 3721 3733 3842 5718
Size: 430 1070 302 195 46 12 109 1876
Cuts at: 5718 1678
Size: 2531
Fragments arranged by size:
2531 1876 1070 430 302 195 109 46
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2379 2411 3060 3092 5212
Size: 32 135 32 807 32 649 32 2120
Cuts at: 5212 5244 5286 5318 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2120 807 649 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3915 4161 669
Size: 988 281 1977 246 3079
Fragments arranged by size:
3079 1977 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6571
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KasI G'GCGC_C
Cuts at: 3174 3671 3729 3174
Size: 497 58 6016
Fragments arranged by size:
6016 497 58
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5685 5869 1305
Size: 4380 184 2007
Fragments arranged by size:
4380 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3362 10
Size: 54 1425 343 1530 3219
Fragments arranged by size:
3219 1530 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3092 4529 4888 5047 518
Size: 648 1926 1437 359 159 2042
Fragments arranged by size:
2042 1926 1437 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2381 2712 3265 3403 3466 3519 3726 3987
Size: 775 331 553 138 63 53 207 261
Cuts at: 3987 4664 5812 6057 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 775 677 553 331 261 245
207 138 63 53
NaeI GCC'GGC
Cuts at: 3173 3429 3173
Size: 256 6315
NarI GG'CG_CC
Cuts at: 3175 3672 3730 3175
Size: 497 58 6016
Fragments arranged by size:
6016 497 58
NcoI C'CATG_G
Cuts at: 513 1065 3191 513
Size: 552 2126 3893
Fragments arranged by size:
3893 2126 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6571
NgoMIV G'CCGG_C
Cuts at: 3171 3427 3171
Size: 256 6315
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 2909 3404 4018 2909
Size: 495 614 5462
Fragments arranged by size:
5462 614 495
NspI r_CATG'y
Cuts at: 1132 1229 2951 3929 5474 1132
Size: 97 1722 978 1545 2229
Fragments arranged by size:
2229 1722 1545 978 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6571
PciI A'CATG_T
Cuts at: 5470 5470
Size: 6571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 3003 1108
Size: 1524 371 4676
Fragments arranged by size:
4676 1524 371
PfoI T'CCnGG_A
Cuts at: 2544 2742 2544
Size: 198 6373
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6153 6185 4640
Size: 32 1481 32 5026
Fragments arranged by size:
5026 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2842 3292 2842
Size: 450 6121
PshAI GACnn'nnGTC
Cuts at: 1613 2381 1613
Size: 768 5803
PsiI TTA'TAA
Cuts at: 4141 4141
Size: 6571
PspOMI G'GGCC_C
Cuts at: 2214 2699 3548 3616 2214
Size: 485 849 68 5169
Fragments arranged by size:
5169 849 485 68
PstI C_TGCA'G
Cuts at: 830 2658 2886 3923 830
Size: 1828 228 1037 3478
Fragments arranged by size:
3478 1828 1037 228
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
PvuII CAG'CTG
Cuts at: 2381 3466 2381
Size: 1085 5486
SacI G_AGCT'C
Cuts at: 3336 3336
Size: 6571
SacII CC_GC'GG
Cuts at: 3404 3727 3404
Size: 323 6248
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SapI GCTCTTCn'nnn_
Cuts at: 2013 3644 2013
Size: 1631 4940
SbfI CC_TGCA'GG
Cuts at: 3923 3923
Size: 6571
ScaI AGT'ACT
Cuts at: 2483 4819 6531 2483
Size: 2336 1712 2523
Fragments arranged by size:
2523 2336 1712
SexAI A'CCwGG_T
Cuts at: 2328 2490 2328
Size: 162 6409
SfcI C'TryA_G
Cuts at: 826 1045 2654 2882 3919 5054 5735 5926 826
Size: 219 1609 228 1037 1135 681 191 1471
Fragments arranged by size:
1609 1471 1135 1037 681 228 219 191
SfoI GGC'GCC
Cuts at: 3176 3673 3731 3176
Size: 497 58 6016
Fragments arranged by size:
6016 497 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 3831 1700
Size: 2131 4440
SmaI CCC'GGG
Cuts at: 1872 3412 3512 3622 3898 1872
Size: 1540 100 110 276 4545
Fragments arranged by size:
4545 1540 276 110 100
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2430 2477 4675 5576 5838 820
Size: 197 926 487 47 2198 901 262 1553
Fragments arranged by size:
2198 1553 926 901 487 262 197 47
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 2951 3929 1132
Size: 1819 978 3774
Fragments arranged by size:
3774 1819 978
SspI AAT'ATT
Cuts at: 5 52 4495 5
Size: 47 4443 2081
Fragments arranged by size:
4443 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6571
StyI C'CwwG_G
Cuts at: 513 1065 3191 4010 513
Size: 552 2126 819 3074
Fragments arranged by size:
3074 2126 819 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3406 4598 4615 4768 4953 5398 325
Size: 1255 1826 1192 17 153 185 445 1498
Fragments arranged by size:
1826 1498 1255 1192 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2481 3858 4817
Size: 274 80 33 51 1443 504 1377 959
Cuts at: 4817 6529 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1377 959 504 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 2922 2954 6531 6563 2228
Size: 32 662 32 3577 32 2236
Fragments arranged by size:
3577 2236 662 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6571
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6571
XmaI C'CCGG_G
Cuts at: 1870 3410 3510 3620 3896 1870
Size: 1540 100 110 276 4545
Fragments arranged by size:
4545 1540 276 110 100
XmnI GAAnn'nnTTC
Cuts at: 4700 4700
Size: 6571
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SfiI
SrfI SwaI XcmI