Restriction map for
pFN21AB7847
(Circular) (Six-base) MAPSORT of: pFN21AB7847.seq Check: 9186 from: 1 to: 6340
pFN21AB7847 6340 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4150 278
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3683 1937
Size: 1746 4594
Acc65I G'GTAC_C
Cuts at: 3661 3661
Size: 6340
AclI AA'CG_TT
Cuts at: 1563 4467 4840 1563
Size: 2904 373 3063
Fragments arranged by size:
3063 2904 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3142 3226 4403 5787 6055 1632
Size: 1510 84 1177 1384 268 1917
Fragments arranged by size:
1917 1510 1384 1177 268 84
AfeI AGC'GCT
Cuts at: 2168 2168
Size: 6340
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6143
AflIII A'CryG_T
Cuts at: 1203 3560 5239 6216 1203
Size: 2357 1679 977 1327
Fragments arranged by size:
2357 1679 1327 977
AgeI A'CCGG_T
Cuts at: 5171 5171
Size: 6340
AhdI GACnn_n'nnGTC
Cuts at: 2044 5069 2044
Size: 3025 3315
AleI CACnn'nnGTG
Cuts at: 1166 3033 3117 3534 1166
Size: 1867 84 417 3972
Fragments arranged by size:
3972 1867 417 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2828 2958 1184
Size: 1644 130 4566
Fragments arranged by size:
4566 1644 130
AlwNI CAG_nnn'CTG
Cuts at: 2281 2308 2903 4083 5655 2281
Size: 27 595 1180 1572 2966
Fragments arranged by size:
2966 1572 1180 595 27
ApaI G_GGCC'C
Cuts at: 2150 2204 2150
Size: 54 6286
ApaLI G'TGCA_C
Cuts at: 4397 5553 4397
Size: 1156 5184
ApoI r'AATT_y
Cuts at: 1493 3276 3334 3649 3875 4027 6136 1493
Size: 1783 58 315 226 152 2109 1697
Fragments arranged by size:
2109 1783 1697 315 226 152 58
AseI AT'TA_AT
Cuts at: 160 1032 4894 160
Size: 872 3862 1606
Fragments arranged by size:
3862 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
AvaI C'yCGr_G
Cuts at: 1870 1943 2142 3665 1870
Size: 73 199 1523 4545
Fragments arranged by size:
4545 1523 199 73
BamHI G'GATC_C
Cuts at: 2552 3670 4037 2552
Size: 1118 367 4855
Fragments arranged by size:
4855 1118 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2419 3661 5116 618
Size: 325 217 627 632 1242 1455 1842
Fragments arranged by size:
1842 1455 1242 632 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2070 2150 2204 3086 3659 1391
Size: 679 80 54 882 573 4072
Fragments arranged by size:
4072 882 679 573 80 54
BbeI G_GCGC'C
Cuts at: 2423 2423
Size: 6340
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2877 3063 3148 3242 928
Size: 971 162 816 186 85 94 4026
Fragments arranged by size:
4026 971 816 186 162 94 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2217 2251 2949 2983 4531 4565 1903
Size: 34 280 34 698 34 1548 34 3678
Fragments arranged by size:
3678 1548 698 280 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4232 5442 4232
Size: 1210 5130
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6340
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 4951 136
Size: 107 122 71 631 605 439 2840 1525
Fragments arranged by size:
2840 1525 631 605 439 122 107 71
BglII A'GATC_T
Cuts at: 6335 6335
Size: 6340
BlpI GC'TnA_GC
Cuts at: 3757 3757
Size: 6340
Bme1580I G_kGCm'C
Cuts at: 1790 2150 2204 4401 5557 1790
Size: 360 54 2197 1156 2573
Fragments arranged by size:
2573 2197 1156 360 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6340
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2038 3210 3294
Size: 416 53 83 399 24 621 1172 84
Cuts at: 3294 4071 5029 442
Size: 777 958 1753
Fragments arranged by size:
1753 1172 958 777 621 416 399 84
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2116 3009 2116
Size: 893 5447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2318 2564 2631 2802 5000 1239
Size: 1064 15 246 67 171 2198 2579
Fragments arranged by size:
2579 2198 1064 246 171 67 15
Bpu10I CC'TnA_GC
Cuts at: 3390 3390
Size: 6340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2475 4465 5330 5628 2475
Size: 1990 865 298 3187
Fragments arranged by size:
3187 1990 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2502 2779 5003 882
Size: 617 1003 277 2224 2219
Fragments arranged by size:
2224 2219 1003 617 277
BsaAI yAC'GTr
Cuts at: 493 1204 4131 493
Size: 711 2927 2702
Fragments arranged by size:
2927 2702 711
BsaBI GATnn'nnATC
Cuts at: 6334 6334
Size: 6340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2420 4147 4529
Size: 53 83 186 936 507 380 1727 382
Cuts at: 4529 5164 275
Size: 635 1451
Fragments arranged by size:
1727 1451 936 635 507 382 380 186
83 53
BsaWI w'CCGG_w
Cuts at: 4773 5171 5445 5592 6003 4773
Size: 398 274 147 411 5110
Fragments arranged by size:
5110 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2680 2710 2906 2936 3436
Size: 30 165 30 1277 30 196 30 500
Cuts at: 3436 3466 1178
Size: 30 4052
Fragments arranged by size:
4052 1277 500 196 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2384 1186
Size: 1198 5142
BseYI C'CCAG_C
Cuts at: 2337 2556 5543 6039 2337
Size: 219 2987 496 2638
Fragments arranged by size:
2987 2638 496 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5767
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3790 4551 5579 1540
Size: 275 186 1789 761 1028 2301
Fragments arranged by size:
2301 1789 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2128 3086 3396 4401 4486 5557 2128
Size: 958 310 1005 85 1071 2911
Fragments arranged by size:
2911 1071 1005 958 310 85
BsmI GAATG_Cn'
Cuts at: 3851 3944 3851
Size: 93 6247
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2128 2150 2204 3086 3396 3659
Size: 399 280 58 22 54 882 310 263
Cuts at: 3659 4401 4486 5557 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 882 742 399 310 280 263
85 58 54 22
BspEI T'CCGG_A
Cuts at: 6003 6003
Size: 6340
BspHI T'CATG_A
Cuts at: 4229 5959 4229
Size: 1730 4610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2416 2910 3695 844
Size: 1572 494 785 3489
Fragments arranged by size:
3489 1572 785 494
BsrBI CCG'CTC
Cuts at: 1394 1823 2364 4227 1394
Size: 429 541 1863 3507
Fragments arranged by size:
3507 1863 541 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2631 2874 4835 5009 65
Size: 1183 1383 243 1961 174 1396
Fragments arranged by size:
1961 1396 1383 1183 243 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2220 2416 4984 5171 1700
Size: 110 410 196 2568 187 2869
Fragments arranged by size:
2869 2568 410 196 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6340
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6340
BssSI C'ACGA_G
Cuts at: 4400 5412 4400
Size: 1012 5328
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2455 794
Size: 1661 4679
BstBI TT'CG_AA
Cuts at: 5144 5144
Size: 6340
BstEII G'GTnAC_C
Cuts at: 1184 2438 1184
Size: 1254 5086
BstXI CCAn_nnnn'nTGG
Cuts at: 2588 2978 2588
Size: 390 5950
BstYI r'GATC_y
Cuts at: 1972 2277 2552 3670 4037 4422 4439 5891 5977
Size: 305 275 1118 367 385 17 1452 86
Cuts at: 5977 5989 6335 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1118 385 367 346 305 275
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2864 2864
Size: 6340
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4849
Fragments arranged by size:
4849 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5601
Fragments arranged by size:
5601 683 56
BtsI GCAGTG_nn'
Cuts at: 3856 4650 4670 3856
Size: 794 20 5526
Fragments arranged by size:
5526 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3295 3330 529
Size: 35 2731 35 3539
Fragments arranged by size:
3539 2731 35 35
DraI TTT'AAA
Cuts at: 3644 3644
Size: 6340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5347 809
Size: 507 4031 1802
Fragments arranged by size:
4031 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3787 4676 6305
Size: 54 1425 50 275 18 1957 889 1629
Cuts at: 6305 8
Size: 43
Fragments arranged by size:
1957 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3787 1537
Size: 275 1975 4090
Fragments arranged by size:
4090 1975 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4270 1353
Size: 2917 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2092 2129 3346 4927 5301 5447
Size: 1064 138 503 37 1217 1581 374 146
Cuts at: 5447 387
Size: 1280
Fragments arranged by size:
1581 1280 1217 1064 503 374 146 138
37
EcoICRI GAG'CTC
Cuts at: 3084 3084
Size: 6340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2318 2564 2631 2802 3142 3226
Size: 393 671 15 246 67 171 340 84
Cuts at: 3226 4403 5000 5787 6055 1239
Size: 1177 597 787 268 1524
Fragments arranged by size:
1524 1177 787 671 597 393 340 268
246 171 84 67 15
EcoO109I rG'GnC_Cy
Cuts at: 2146 2147 2201 2652 2146
Size: 1 54 451 5834
Fragments arranged by size:
5834 451 54 1
EcoRI G'AATT_C
Cuts at: 3334 3649 4027 3334
Size: 315 378 5647
Fragments arranged by size:
5647 378 315
FspI TGC'GCA
Cuts at: 4846 4846
Size: 6340
HaeII r_GCGC'y
Cuts at: 1678 2170 2423 5487 1678
Size: 492 253 3064 2531
Fragments arranged by size:
3064 2531 492 253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2473 2505 2680
Size: 32 135 32 458 32 411 32 175
Cuts at: 2680 2712 4981 5013 5055 5087 1373
Size: 32 2269 32 42 32 2626
Fragments arranged by size:
2626 2269 458 411 175 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3684 3930 669
Size: 988 281 1746 246 3079
Fragments arranged by size:
3079 1746 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2828 748
Size: 2080 4260
HpaI GTT'AAC
Cuts at: 3930 3930
Size: 6340
KasI G'GCGC_C
Cuts at: 2419 2419
Size: 6340
KpnI G_GTAC'C
Cuts at: 3665 3665
Size: 6340
MfeI C'AATT_G
Cuts at: 3939 3939
Size: 6340
MluI A'CGCG_T
Cuts at: 6216 6216
Size: 6340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5454 5638 1305
Size: 4149 184 2007
Fragments arranged by size:
4149 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4518
Fragments arranged by size:
4518 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2976 3033 3117 3133 3301 3534 4298
Size: 648 1810 57 84 16 168 233 764
Cuts at: 4298 4657 4816 518
Size: 359 159 2042
Fragments arranged by size:
2042 1810 764 648 359 233 168 159
84 57 16
MspA1I CmG'CkG
Cuts at: 1606 2407 2713 3756 4433 5581 5826 1606
Size: 801 306 1043 677 1148 245 2120
Fragments arranged by size:
2120 1148 1043 801 677 306 245
NaeI GCC'GGC
Cuts at: 2222 2418 2222
Size: 196 6144
NarI GG'CG_CC
Cuts at: 2420 2420
Size: 6340
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4849
Fragments arranged by size:
4849 939 552
NdeI CA'TA_TG
Cuts at: 387 3130 387
Size: 2743 3597
NgoMIV G'CCGG_C
Cuts at: 2220 2416 2220
Size: 196 6144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6340
NotI GC'GGCC_GC
Cuts at: 3787 3787
Size: 6340
NspI r_CATG'y
Cuts at: 1132 1229 2897 3564 3698 5243 1132
Size: 97 1668 667 134 1545 2229
Fragments arranged by size:
2229 1668 1545 667 134 97
PasI CC'CwG_GG
Cuts at: 1348 2599 2656 1348
Size: 1251 57 5032
Fragments arranged by size:
5032 1251 57
PciI A'CATG_T
Cuts at: 3560 5239 3560
Size: 1679 4661
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6340
PfoI T'CCnGG_A
Cuts at: 2280 2280
Size: 6340
PmeI GTTT'AAAC
Cuts at: 3644 3644
Size: 6340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4409 4441 5922 5954 4409
Size: 32 1481 32 4795
Fragments arranged by size:
4795 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2652 2652
Size: 6340
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6340
PsiI TTA'TAA
Cuts at: 3465 3910 3465
Size: 445 5895
PspOMI G'GGCC_C
Cuts at: 2146 2200 2146
Size: 54 6286
PstI C_TGCA'G
Cuts at: 830 2053 2135 2253 2334 2615 3257 3692 830
Size: 1223 82 118 81 281 642 435 3478
Fragments arranged by size:
3478 1223 642 435 281 118 82 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6340
PvuII CAG'CTG
Cuts at: 2407 2713 2407
Size: 306 6034
SacI G_AGCT'C
Cuts at: 3086 3086
Size: 6340
SalI G'TCGA_C
Cuts at: 1936 3682 1936
Size: 1746 4594
SanDI GG'GwC_CC
Cuts at: 2652 2652
Size: 6340
SbfI CC_TGCA'GG
Cuts at: 3692 3692
Size: 6340
ScaI AGT'ACT
Cuts at: 4588 6300 4588
Size: 1712 4628
SfcI C'TryA_G
Cuts at: 826 1045 2049 2131 2249 2330 2611 2737 3253
Size: 219 1004 82 118 81 281 126 516
Cuts at: 3253 3688 4823 5504 5695 826
Size: 435 1135 681 191 1471
Fragments arranged by size:
1471 1135 1004 681 516 435 281 219
191 126 118 82 81
SfoI GGC'GCC
Cuts at: 2421 2421
Size: 6340
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6340
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6340
SmaI CCC'GGG
Cuts at: 1872 3667 1872
Size: 1795 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2490 4444 5345 5607 820
Size: 197 926 547 1954 901 262 1553
Fragments arranged by size:
1954 1553 926 901 547 262 197
SnaBI TAC'GTA
Cuts at: 493 4131 493
Size: 3638 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6340
SphI G_CATG'C
Cuts at: 1132 3698 1132
Size: 2566 3774
SspI AAT'ATT
Cuts at: 5 52 4264 5
Size: 47 4212 2081
Fragments arranged by size:
4212 2081 47
StuI AGG'CCT
Cuts at: 1517 3006 1517
Size: 1489 4851
StyI C'CwwG_G
Cuts at: 513 1065 2004 2779 3779 513
Size: 552 939 775 1000 3074
Fragments arranged by size:
3074 1000 939 775 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2329 2691 4367 4384 4537 4722 5167
Size: 1255 749 362 1676 17 153 185 445
Cuts at: 5167 325
Size: 1498
Fragments arranged by size:
1676 1498 1255 749 445 362 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4586 6298 96
Size: 274 80 33 51 1443 2609 1712 138
Fragments arranged by size:
2609 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3352 3384 3386 3418 3419 3451 6300 6332 3352
Size: 32 2 32 1 32 2849 32 3360
Fragments arranged by size:
3360 2849 32 32 32 32 2 1
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6340
XbaI T'CTAG_A
Cuts at: 3676 3676
Size: 6340
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6340
XmaI C'CCGG_G
Cuts at: 1870 3665 1870
Size: 1795 4545
XmnI GAAnn'nnTTC
Cuts at: 3239 4469 3239
Size: 1230 5110
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4148 276
Size: 53 83 186 936 2614 2468
Fragments arranged by size:
2614 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SapI SexAI SfiI SrfI SwaI XcmI