Restriction map for
pFN21AB7846
(Circular) (Six-base) MAPSORT of: pFN21AB7846.seq Check: 9409 from: 1 to: 6565
pFN21AB7846 6565 bp
With 182 enzymes: SgfI *
November 30, 2011 11:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2702 3049 2702
Size: 347 6218
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2262 2291 3105 4375
Size: 53 83 186 936 726 29 814 1270
Cuts at: 4375 278
Size: 2468
Fragments arranged by size:
2468 1270 936 814 726 186 83 53
29
AccI GT'mk_AC
Cuts at: 1937 3908 1937
Size: 1971 4594
Acc65I G'GTAC_C
Cuts at: 3886 3886
Size: 6565
AclI AA'CG_TT
Cuts at: 1563 4692 5065 1563
Size: 3129 373 3063
Fragments arranged by size:
3129 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2459 2896 3422 3786 4628 6012 6280
Size: 578 249 437 526 364 842 1384 268
Cuts at: 6280 1632
Size: 1917
Fragments arranged by size:
1917 1384 842 578 526 437 364 268
249
AfeI AGC'GCT
Cuts at: 2999 3747 2999
Size: 748 5817
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6368
AflIII A'CryG_T
Cuts at: 1203 3138 5464 6441 1203
Size: 1935 2326 977 1327
Fragments arranged by size:
2326 1935 1327 977
AgeI A'CCGG_T
Cuts at: 5396 5396
Size: 6565
AhdI GACnn_n'nnGTC
Cuts at: 3114 5294 3114
Size: 2180 4385
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6565
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3713 1184
Size: 2529 4036
AlwNI CAG_nnn'CTG
Cuts at: 2494 3196 4308 5880 2494
Size: 702 1112 1572 3179
Fragments arranged by size:
3179 1572 1112 702
ApaI G_GGCC'C
Cuts at: 2048 2351 2365 3086 3372 2048
Size: 303 14 721 286 5241
Fragments arranged by size:
5241 721 303 286 14
ApaLI G'TGCA_C
Cuts at: 2768 4622 5778 2768
Size: 1854 1156 3555
Fragments arranged by size:
3555 1854 1156
ApoI r'AATT_y
Cuts at: 1493 3874 4100 4252 6361 1493
Size: 2381 226 152 2109 1697
Fragments arranged by size:
2381 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5119 160
Size: 872 4087 1606
Fragments arranged by size:
4087 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
AvaI C'yCGr_G
Cuts at: 1870 1943 2083 2162 2177 2773 2893 3354 3788
Size: 73 140 79 15 596 120 461 434
Cuts at: 3788 3890 1870
Size: 102 4545
Fragments arranged by size:
4545 596 461 434 140 120 102 79
73 15
BamHI G'GATC_C
Cuts at: 3895 4262 3895
Size: 367 6198
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3285 3886 5341 618
Size: 325 217 627 1498 601 1455 1842
Fragments arranged by size:
1842 1498 1455 627 601 325 217
BanII G_rGCy'C
Cuts at: 1391 2048 2324 2351 2365 2472 3086 3372 3512
Size: 657 276 27 14 107 614 286 140
Cuts at: 3512 3884 1391
Size: 372 4072
Fragments arranged by size:
4072 657 614 372 286 276 140 107
27 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5594
BbvCI CC'TCA_GC
Cuts at: 3686 3726 3686
Size: 40 6525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4756 4790 1903
Size: 34 2819 34 3678
Fragments arranged by size:
3678 2819 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3222 4457 5667 3222
Size: 1235 1210 4120
Fragments arranged by size:
4120 1235 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6565
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2397 2544 2565
Size: 107 122 71 631 605 725 147 21
Cuts at: 2565 2865 5176 136
Size: 300 2311 1525
Fragments arranged by size:
2311 1525 725 631 605 300 147 122
107 71 21
BglII A'GATC_T
Cuts at: 2338 6560 2338
Size: 4222 2343
BlpI GC'TnA_GC
Cuts at: 3982 3982
Size: 6565
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2351 2365 2772 3086 3372 4626 5782
Size: 258 303 14 407 314 286 1254 1156
Cuts at: 5782 1790
Size: 2573
Fragments arranged by size:
2573 1254 1156 407 314 303 286 258
14
BmgBI CAC'GTC
Cuts at: 1318 3031 3141 1318
Size: 1713 110 4742
Fragments arranged by size:
4742 1713 110
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4296 5254 442
Size: 416 53 83 399 24 2879 958 1753
Fragments arranged by size:
2879 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6565
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2343 3465 2343
Size: 1122 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2852 2905 5225 1239
Size: 902 711 53 2320 2579
Fragments arranged by size:
2579 2320 902 711 53
Bpu10I CC'TnA_GC
Cuts at: 3686 3726 3686
Size: 40 6525
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2825 4690 5555 5853 2825
Size: 1865 865 298 3537
Fragments arranged by size:
3537 1865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2159 3635 5228 882
Size: 617 660 1476 1593 2219
Fragments arranged by size:
2219 1593 1476 660 617
BsaAI yAC'GTr
Cuts at: 493 1204 4356 493
Size: 711 3152 2702
Fragments arranged by size:
3152 2702 711
BsaBI GATnn'nnATC
Cuts at: 6559 6559
Size: 6565
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2259 2288 3102 4372
Size: 53 83 186 936 726 29 814 1270
Cuts at: 4372 4754 5389 275
Size: 382 635 1451
Fragments arranged by size:
1451 1270 936 814 726 635 382 186
83 53 29
BsaWI w'CCGG_w
Cuts at: 2316 3597 4998 5396 5670 5817 6228 2316
Size: 1281 1401 398 274 147 411 2653
Fragments arranged by size:
2653 1401 1281 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2249 2279 2999 3029 3830
Size: 30 165 30 846 30 720 30 801
Cuts at: 3830 3860 1178
Size: 30 3883
Fragments arranged by size:
3883 846 801 720 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2224 2227 2242 2269 2648 2869 3368 1186
Size: 1038 3 15 27 379 221 499 4383
Fragments arranged by size:
4383 1038 499 379 221 27 15 3
BseYI C'CCAG_C
Cuts at: 2138 2227 2554 2598 3011 3262 3409 3479 3530
Size: 89 327 44 413 251 147 70 51
Cuts at: 3530 5768 6264 2138
Size: 2238 496 2439
Fragments arranged by size:
2439 2238 496 413 327 251 147 89
70 51 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2048 2634 1163
Size: 573 312 586 5094
Fragments arranged by size:
5094 586 573 312
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 4015 4776 5804 1540
Size: 275 186 928 1086 761 1028 2301
Fragments arranged by size:
2301 1086 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2472 2772 3353 4626 4711 5782 2472
Size: 300 581 1273 85 1071 3255
Fragments arranged by size:
3255 1273 1071 581 300 85
BsiWI C'GTAC_G
Cuts at: 2967 2967
Size: 6565
BsmI GAATG_Cn'
Cuts at: 4076 4169 4076
Size: 93 6472
BsmBI CGTCTCn'nnnn_
Cuts at: 2295 2295
Size: 6565
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2324 2351 2365 2472 2772 3086
Size: 399 258 276 27 14 107 300 314
Cuts at: 3086 3353 3372 3512 3884 4626 4711 5782 1391
Size: 267 19 140 372 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 314 300 276
267 258 140 107 85 27 19 14
BspEI T'CCGG_A
Cuts at: 2316 6228 2316
Size: 3912 2653
BspHI T'CATG_A
Cuts at: 4454 6184 4454
Size: 1730 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 3049 3920 844
Size: 1858 347 871 3489
Fragments arranged by size:
3489 1858 871 347
BsrBI CCG'CTC
Cuts at: 1394 1823 2277 4452 1394
Size: 429 454 2175 3507
Fragments arranged by size:
3507 2175 454 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5060 5234 65
Size: 1183 3812 174 1396
Fragments arranged by size:
3812 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3617 5209 5396 1700
Size: 110 1807 1592 187 2869
Fragments arranged by size:
2869 1807 1592 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6565
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6565
BssSI C'ACGA_G
Cuts at: 4625 5637 4625
Size: 1012 5553
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2494 3061 794
Size: 1700 567 4298
Fragments arranged by size:
4298 1700 567
BstBI TT'CG_AA
Cuts at: 5369 5369
Size: 6565
BstEII G'GTnAC_C
Cuts at: 1184 2296 3542 1184
Size: 1112 1246 4207
Fragments arranged by size:
4207 1246 1112
BstXI CCAn_nnnn'nTGG
Cuts at: 3151 3151
Size: 6565
BstYI r'GATC_y
Cuts at: 1972 2338 2844 2979 3895 4262 4647 4664 6116
Size: 366 506 135 916 367 385 17 1452
Cuts at: 6116 6202 6214 6560 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 916 506 385 367 366 346
135 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2852 2852
Size: 6565
BtgI C'CryG_G
Cuts at: 513 1065 2004 2451 3339 3646 513
Size: 552 939 447 888 307 3432
Fragments arranged by size:
3432 939 888 552 447 307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5826
Fragments arranged by size:
5826 683 56
BtsI GCAGTG_nn'
Cuts at: 3826 4081 4875 4895 3826
Size: 255 794 20 5496
Fragments arranged by size:
5496 794 255 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6565
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6530
DraI TTT'AAA
Cuts at: 3869 3869
Size: 6565
DraIII CAC_nnn'GTG
Cuts at: 2246 2246
Size: 6565
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3632 5572 809
Size: 507 2316 1940 1802
Fragments arranged by size:
2316 1940 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2577 4012
Size: 54 1425 50 275 18 222 525 1435
Cuts at: 4012 4901 6530 8
Size: 889 1629 43
Fragments arranged by size:
1629 1435 1425 889 525 275 222 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4012 1537
Size: 275 2200 4090
Fragments arranged by size:
4090 2200 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2651 4495 1353
Size: 1298 1844 3423
Fragments arranged by size:
3423 1844 1298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5152 5526 5672 387
Size: 1064 138 3563 374 146 1280
Fragments arranged by size:
3563 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2470 2470
Size: 6565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2210 2459 2852 2896 2905 3422
Size: 393 509 69 249 393 44 9 517
Cuts at: 3422 3786 4628 5225 6012 6280 1239
Size: 364 842 597 787 268 1524
Fragments arranged by size:
1524 842 787 597 517 509 393 393
364 268 249 69 44 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2177 2441 3188 3705 3725 2177
Size: 264 747 517 20 5017
Fragments arranged by size:
5017 747 517 264 20
EcoO109I rG'GnC_Cy
Cuts at: 2044 2347 2348 2491 3083 3368 3718 2044
Size: 303 1 143 592 285 350 4891
Fragments arranged by size:
4891 592 350 303 285 143 1
EcoRI G'AATT_C
Cuts at: 3874 4252 3874
Size: 378 6187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 6565
FspI TGC'GCA
Cuts at: 5071 5071
Size: 6565
HaeII r_GCGC'y
Cuts at: 1678 3001 3749 5712 1678
Size: 1323 748 1963 2531
Fragments arranged by size:
2531 1963 1323 748
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 2323 2355 2999
Size: 32 135 32 677 32 42 32 644
Cuts at: 2999 3031 3100 3132 3761 3793 5206 5238 5280
Size: 32 69 32 629 32 1413 32 42
Cuts at: 5280 5312 1373
Size: 32 2626
Fragments arranged by size:
2626 1413 677 644 629 135 69 42
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3909 4155 669
Size: 988 281 1971 246 3079
Fragments arranged by size:
3079 1971 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6565
HpaI GTT'AAC
Cuts at: 4155 4155
Size: 6565
KpnI G_GTAC'C
Cuts at: 3890 3890
Size: 6565
MfeI C'AATT_G
Cuts at: 4164 4164
Size: 6565
MluI A'CGCG_T
Cuts at: 6441 6441
Size: 6565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2280 5679 5863 1305
Size: 975 3399 184 2007
Fragments arranged by size:
3399 2007 975 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2054 10
Size: 54 1425 343 222 4521
Fragments arranged by size:
4521 1425 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2759 2765 4523 4882 5041 518
Size: 648 1593 6 1758 359 159 2042
Fragments arranged by size:
2042 1758 1593 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2050 2453 2598 3151 3295 3424 3783 3981
Size: 444 403 145 553 144 129 359 198
Cuts at: 3981 4658 5806 6051 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 553 444 403 359 245
198 145 144 129
NcoI C'CATG_G
Cuts at: 513 1065 2004 3339 513
Size: 552 939 1335 3739
Fragments arranged by size:
3739 1335 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6565
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6565
NotI GC'GGCC_GC
Cuts at: 4012 4012
Size: 6565
NspI r_CATG'y
Cuts at: 1132 1229 2764 2800 3080 3923 5468 1132
Size: 97 1535 36 280 843 1545 2229
Fragments arranged by size:
2229 1545 1535 843 280 97 36
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6565
PciI A'CATG_T
Cuts at: 5464 5464
Size: 6565
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2540 3295 3832 1108
Size: 1432 755 537 3841
Fragments arranged by size:
3841 1432 755 537
PmeI GTTT'AAAC
Cuts at: 3869 3869
Size: 6565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4634 4666 6147 6179 4634
Size: 32 1481 32 5020
Fragments arranged by size:
5020 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6565
PsiI TTA'TAA
Cuts at: 4135 4135
Size: 6565
PspOMI G'GGCC_C
Cuts at: 2044 2347 2361 3082 3368 2044
Size: 303 14 721 286 5241
Fragments arranged by size:
5241 721 303 286 14
PstI C_TGCA'G
Cuts at: 830 2813 3129 3917 830
Size: 1983 316 788 3478
Fragments arranged by size:
3478 1983 788 316
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6565
PvuII CAG'CTG
Cuts at: 3151 3151
Size: 6565
SacI G_AGCT'C
Cuts at: 2472 2472
Size: 6565
SacII CC_GC'GG
Cuts at: 2454 2454
Size: 6565
SalI G'TCGA_C
Cuts at: 1936 3907 1936
Size: 1971 4594
SapI GCTCTTCn'nnn_
Cuts at: 2651 2651
Size: 6565
SbfI CC_TGCA'GG
Cuts at: 3917 3917
Size: 6565
ScaI AGT'ACT
Cuts at: 4813 6525 4813
Size: 1712 4853
SfcI C'TryA_G
Cuts at: 826 1045 2809 3125 3913 5048 5729 5920 826
Size: 219 1764 316 788 1135 681 191 1471
Fragments arranged by size:
1764 1471 1135 788 681 316 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6565
SmaI CCC'GGG
Cuts at: 1872 2085 2895 3356 3892 1872
Size: 213 810 461 536 4545
Fragments arranged by size:
4545 810 536 461 213
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2840 4669 5570 5832 820
Size: 197 926 897 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 897 262 197
SnaBI TAC'GTA
Cuts at: 493 4356 493
Size: 3863 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6565
SphI G_CATG'C
Cuts at: 1132 3080 3923 1132
Size: 1948 843 3774
Fragments arranged by size:
3774 1948 843
SspI AAT'ATT
Cuts at: 5 52 4489 5
Size: 47 4437 2081
Fragments arranged by size:
4437 2081 47
StuI AGG'CCT
Cuts at: 1517 2547 2819 2909 3731 1517
Size: 1030 272 90 822 4351
Fragments arranged by size:
4351 1030 822 272 90
StyI C'CwwG_G
Cuts at: 513 1065 2004 2539 3339 4004 513
Size: 552 939 535 800 665 3074
Fragments arranged by size:
3074 939 800 665 552 535
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2082 2590 2963 3125 4592 4609 4762
Size: 1255 502 508 373 162 1467 17 153
Cuts at: 4762 4947 5392 325
Size: 185 445 1498
Fragments arranged by size:
1498 1467 1255 508 502 445 373 185
162 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4811 6523 96
Size: 274 80 33 51 1443 2834 1712 138
Fragments arranged by size:
2834 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3311 3343 3830 3862 6525 6557 3311
Size: 32 487 32 2663 32 3319
Fragments arranged by size:
3319 2663 487 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6565
XbaI T'CTAG_A
Cuts at: 3901 3901
Size: 6565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2146 2146
Size: 6565
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6565
XmaI C'CCGG_G
Cuts at: 1870 2083 2893 3354 3890 1870
Size: 213 810 461 536 4545
Fragments arranged by size:
4545 810 536 461 213
XmnI GAAnn'nnTTC
Cuts at: 2909 4694 2909
Size: 1785 4780
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2260 2289 3103 4373
Size: 53 83 186 936 726 29 814 1270
Cuts at: 4373 276
Size: 2468
Fragments arranged by size:
2468 1270 936 814 726 186 83 53
29
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BstZ17I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI
SexAI SfiI SfoI SrfI SwaI