Restriction map for
pFN21AB7845
(Circular) (Six-base) MAPSORT of: pFN21AB7845.seq Check: 9181 from: 1 to: 6337
pFN21AB7845 6337 bp
With 182 enzymes: SgfI *
March 28, 2012 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3334 3348 3392 3334
Size: 14 44 6279
Fragments arranged by size:
6279 44 14
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4147 278
Size: 53 83 186 936 2611 2468
Fragments arranged by size:
2611 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3680 1937
Size: 1743 4594
Acc65I G'GTAC_C
Cuts at: 2534 3658 2534
Size: 1124 5213
AclI AA'CG_TT
Cuts at: 1563 4464 4837 1563
Size: 2901 373 3063
Fragments arranged by size:
3063 2901 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3223 3307 3388 4400 5784 6052 1632
Size: 1591 84 81 1012 1384 268 1917
Fragments arranged by size:
1917 1591 1384 1012 268 84 81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6140
AflIII A'CryG_T
Cuts at: 1203 3213 5236 6213 1203
Size: 2010 2023 977 1327
Fragments arranged by size:
2023 2010 1327 977
AgeI A'CCGG_T
Cuts at: 5168 5168
Size: 6337
AhdI GACnn_n'nnGTC
Cuts at: 5066 5066
Size: 6337
AleI CACnn'nnGTG
Cuts at: 1166 3435 1166
Size: 2269 4068
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3453 1184
Size: 2269 4068
AlwNI CAG_nnn'CTG
Cuts at: 2248 3340 4080 5652 2248
Size: 1092 740 1572 2933
Fragments arranged by size:
2933 1572 1092 740
ApaI G_GGCC'C
Cuts at: 2915 2915
Size: 6337
ApaLI G'TGCA_C
Cuts at: 3263 3347 3428 4394 5550 3263
Size: 84 81 966 1156 4050
Fragments arranged by size:
4050 1156 966 84 81
ApoI r'AATT_y
Cuts at: 1493 3646 3872 4024 6133 1493
Size: 2153 226 152 2109 1697
Fragments arranged by size:
2153 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4891 160
Size: 872 3859 1606
Fragments arranged by size:
3859 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6337
AvaI C'yCGr_G
Cuts at: 1870 1943 2222 3662 1870
Size: 73 279 1440 4545
Fragments arranged by size:
4545 1440 279 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2518 2551 2518
Size: 33 6304
BamHI G'GATC_C
Cuts at: 2307 3667 4034 2307
Size: 1360 367 4610
Fragments arranged by size:
4610 1360 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2534 2993 3099 3465 3597
Size: 325 217 627 747 459 106 366 132
Cuts at: 3597 3658 5113 618
Size: 61 1455 1842
Fragments arranged by size:
1842 1455 747 627 459 366 325 217
132 106 61
BanII G_rGCy'C
Cuts at: 1391 2193 2915 2922 3086 3656 1391
Size: 802 722 7 164 570 4072
Fragments arranged by size:
4072 802 722 570 164 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2856 3611 928
Size: 971 957 755 3654
Fragments arranged by size:
3654 971 957 755
BbvCI CC'TCA_GC
Cuts at: 2040 2132 2216 2040
Size: 92 84 6161
Fragments arranged by size:
6161 92 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4528 4562 1903
Size: 34 50 34 2507 34 3678
Fragments arranged by size:
3678 2507 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4229 5439 4229
Size: 1210 5127
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6337
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2958 3422 4948
Size: 107 122 71 631 605 1286 464 1526
Cuts at: 4948 136
Size: 1525
Fragments arranged by size:
1526 1525 1286 631 605 464 122 107
71
BglII A'GATC_T
Cuts at: 6332 6332
Size: 6337
BlpI GC'TnA_GC
Cuts at: 3754 3754
Size: 6337
Bme1580I G_kGCm'C
Cuts at: 1790 2915 3056 3267 3351 3432 3442 4398 5554
Size: 1125 141 211 84 81 10 956 1156
Cuts at: 5554 1790
Size: 2573
Fragments arranged by size:
2573 1156 1125 956 211 141 84 81
10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6337
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4068 5026
Size: 416 53 83 399 24 1874 777 958
Cuts at: 5026 442
Size: 1753
Fragments arranged by size:
1874 1753 958 777 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2177 2270 2285 2399 2583 2790 2891
Size: 854 84 93 15 114 184 207 101
Cuts at: 2891 3278 4997 1239
Size: 387 1719 2579
Fragments arranged by size:
2579 1719 854 387 207 184 114 101
93 84 15
Bpu10I CC'TnA_GC
Cuts at: 2040 2132 2216 2040
Size: 92 84 6161
Fragments arranged by size:
6161 92 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4462 5327 5625 4462
Size: 865 298 5174
Fragments arranged by size:
5174 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2637 2806 5000 882
Size: 617 1138 169 2194 2219
Fragments arranged by size:
2219 2194 1138 617 169
BsaAI yAC'GTr
Cuts at: 493 1204 3214 4128 493
Size: 711 2010 914 2702
Fragments arranged by size:
2702 2010 914 711
BsaBI GATnn'nnATC
Cuts at: 6331 6331
Size: 6337
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4144 4526 5161 275
Size: 53 83 186 936 2611 382 635 1451
Fragments arranged by size:
2611 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3351 4770 5168 5442 5589 6000 3351
Size: 1419 398 274 147 411 3688
Fragments arranged by size:
3688 1419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 1178
Size: 30 165 30 663 30 5419
Fragments arranged by size:
5419 663 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2028 2555 2741 2744 2970 1186
Size: 842 527 186 3 226 4553
Fragments arranged by size:
4553 842 527 226 186 3
BseYI C'CCAG_C
Cuts at: 2464 2660 2749 2985 3609 5540 6036 2464
Size: 196 89 236 624 1931 496 2765
Fragments arranged by size:
2765 1931 624 496 236 196 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3238 3653 1163
Size: 573 1502 415 3847
Fragments arranged by size:
3847 1502 573 415
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3787 4548 5576 1540
Size: 275 186 1786 761 1028 2301
Fragments arranged by size:
2301 1786 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 2377 3086 3267 3351 3432 4398 4483 5554
Size: 184 709 181 84 81 966 85 1071
Cuts at: 5554 2193
Size: 2976
Fragments arranged by size:
2976 1071 966 709 184 181 85 84
81
BsmI GAATG_Cn'
Cuts at: 3418 3848 3941 3418
Size: 430 93 5814
Fragments arranged by size:
5814 430 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2377 2915 2922 3056 3086 3267
Size: 399 403 184 538 7 134 30 181
Cuts at: 3267 3351 3432 3442 3656 4398 4483 5554 1391
Size: 84 81 10 214 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 538 403 399 214 184
181 134 85 84 81 30 10 7
BspEI T'CCGG_A
Cuts at: 6000 6000
Size: 6337
BspHI T'CATG_A
Cuts at: 4226 5956 4226
Size: 1730 4607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3334 3348 3392 3625 3692 844
Size: 2490 14 44 233 67 3489
Fragments arranged by size:
3489 2490 233 67 44 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3502 4224 1394
Size: 429 1679 722 3507
Fragments arranged by size:
3507 1679 722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4832 5006 65
Size: 1183 3584 174 1396
Fragments arranged by size:
3584 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3432 3594 4981 5168 1700
Size: 110 1622 162 1387 187 2869
Fragments arranged by size:
2869 1622 1387 187 162 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6337
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6337
BssSI C'ACGA_G
Cuts at: 4397 5409 4397
Size: 1012 5325
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2963 3225 3340 794
Size: 2169 262 115 3791
Fragments arranged by size:
3791 2169 262 115
BstBI TT'CG_AA
Cuts at: 5141 5141
Size: 6337
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6337
BstXI CCAn_nnnn'nTGG
Cuts at: 2915 2915
Size: 6337
BstYI r'GATC_y
Cuts at: 1972 2244 2307 3667 4034 4419 4436 5888 5974
Size: 272 63 1360 367 385 17 1452 86
Cuts at: 5974 5986 6332 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1360 385 367 346 272 86
63 17 12
Bsu36I CC'TnA_GG
Cuts at: 2724 2724
Size: 6337
BtgI C'CryG_G
Cuts at: 513 1065 2087 2349 513
Size: 552 1022 262 4501
Fragments arranged by size:
4501 1022 552 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5598
Fragments arranged by size:
5598 683 56
BtsI GCAGTG_nn'
Cuts at: 2637 2968 3853 4647 4667 2637
Size: 331 885 794 20 4307
Fragments arranged by size:
4307 885 794 331 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6337
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6302
DraI TTT'AAA
Cuts at: 2701 3641 2701
Size: 940 5397
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2798 5344 809
Size: 507 1482 2546 1802
Fragments arranged by size:
2546 1802 1482 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 3784 4673
Size: 54 1425 50 275 18 513 1441 889
Cuts at: 4673 6302 8
Size: 1629 43
Fragments arranged by size:
1629 1441 1425 889 513 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3784 1537
Size: 275 1972 4090
Fragments arranged by size:
4090 1972 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3111 3311 4267 1353
Size: 1758 200 956 3423
Fragments arranged by size:
3423 1758 956 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2045 4924 5298 5444 387
Size: 1064 138 456 2879 374 146 1280
Fragments arranged by size:
2879 1280 1064 456 374 146 138
EcoICRI GAG'CTC
Cuts at: 2191 3084 2191
Size: 893 5444
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2177 2270 2285 2399 2583 2790
Size: 393 461 84 93 15 114 184 207
Cuts at: 2790 2891 3223 3278 3307 3388 4400 4997 5784
Size: 101 332 55 29 81 1012 597 787
Cuts at: 5784 6052 1239
Size: 268 1524
Fragments arranged by size:
1524 1012 787 597 461 393 332 268
207 184 114 101 93 84 81 55
29 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2508 2508
Size: 6337
EcoO109I rG'GnC_Cy
Cuts at: 2107 2338 2359 2557 2615 2911 2912 3207 2107
Size: 231 21 198 58 296 1 295 5237
Fragments arranged by size:
5237 296 295 231 198 58 21 1
EcoRI G'AATT_C
Cuts at: 3646 4024 3646
Size: 378 5959
FspI TGC'GCA
Cuts at: 4843 4843
Size: 6337
HaeII r_GCGC'y
Cuts at: 1678 2445 2867 5484 1678
Size: 767 422 2617 2531
Fragments arranged by size:
2617 2531 767 422
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3073 3105 4978 5010 5052
Size: 32 135 32 1501 32 1873 32 42
Cuts at: 5052 5084 1373
Size: 32 2626
Fragments arranged by size:
2626 1873 1501 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3681 3927 669
Size: 988 281 1743 246 3079
Fragments arranged by size:
3079 1743 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3568 748
Size: 2820 3517
HpaI GTT'AAC
Cuts at: 3927 3927
Size: 6337
KpnI G_GTAC'C
Cuts at: 2538 3662 2538
Size: 1124 5213
MfeI C'AATT_G
Cuts at: 3936 3936
Size: 6337
MluI A'CGCG_T
Cuts at: 6213 6213
Size: 6337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3059 3535 5451 5635 1305
Size: 1754 476 1916 184 2007
Fragments arranged by size:
2007 1916 1754 476 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4515
Fragments arranged by size:
4515 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3435 3463 4295 4654 4813 518
Size: 648 2269 28 832 359 159 2042
Fragments arranged by size:
2269 2042 832 648 359 159 28
MspA1I CmG'CkG
Cuts at: 1606 2165 2464 2660 3628 3753 4430 5578 5823
Size: 559 299 196 968 125 677 1148 245
Cuts at: 5823 1606
Size: 2120
Fragments arranged by size:
2120 1148 968 677 559 299 245 196
125
NcoI C'CATG_G
Cuts at: 513 1065 2087 2349 513
Size: 552 1022 262 4501
Fragments arranged by size:
4501 1022 552 262
NdeI CA'TA_TG
Cuts at: 387 3460 387
Size: 3073 3264
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6337
NotI GC'GGCC_GC
Cuts at: 3784 3784
Size: 6337
NspI r_CATG'y
Cuts at: 1132 1229 2744 3695 5240 1132
Size: 97 1515 951 1545 2229
Fragments arranged by size:
2229 1545 1515 951 97
PasI CC'CwG_GG
Cuts at: 1348 2504 2915 1348
Size: 1156 411 4770
Fragments arranged by size:
4770 1156 411
PciI A'CATG_T
Cuts at: 5236 5236
Size: 6337
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2330 2653 2813 1108
Size: 1222 323 160 4632
Fragments arranged by size:
4632 1222 323 160
PfoI T'CCnGG_A
Cuts at: 2193 2247 2310 2583 2193
Size: 54 63 273 5947
Fragments arranged by size:
5947 273 63 54
PmeI GTTT'AAAC
Cuts at: 3641 3641
Size: 6337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4406 4438 5919 5951 4406
Size: 32 1481 32 4792
Fragments arranged by size:
4792 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 2359 2557 2615 2107
Size: 252 198 58 5829
Fragments arranged by size:
5829 252 198 58
PshAI GACnn'nnGTC
Cuts at: 1613 2112 1613
Size: 499 5838
PsiI TTA'TAA
Cuts at: 3907 3907
Size: 6337
PspOMI G'GGCC_C
Cuts at: 2911 2911
Size: 6337
PstI C_TGCA'G
Cuts at: 830 2610 2798 2959 3345 3689 830
Size: 1780 188 161 386 344 3478
Fragments arranged by size:
3478 1780 386 344 188 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6337
PvuII CAG'CTG
Cuts at: 2165 2464 2660 2165
Size: 299 196 5842
Fragments arranged by size:
5842 299 196
SacI G_AGCT'C
Cuts at: 2193 3086 2193
Size: 893 5444
SalI G'TCGA_C
Cuts at: 1936 3679 1936
Size: 1743 4594
SapI GCTCTTCn'nnn_
Cuts at: 3311 3311
Size: 6337
SbfI CC_TGCA'GG
Cuts at: 2959 3345 3689 2959
Size: 386 344 5607
Fragments arranged by size:
5607 386 344
ScaI AGT'ACT
Cuts at: 4585 6297 4585
Size: 1712 4625
SfcI C'TryA_G
Cuts at: 826 1045 2606 2794 2955 3341 3685 4820 5501
Size: 219 1561 188 161 386 344 1135 681
Cuts at: 5501 5692 826
Size: 191 1471
Fragments arranged by size:
1561 1471 1135 681 386 344 219 191
188 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6337
SgrAI Cr'CCGG_yG
Cuts at: 1700 3432 1700
Size: 1732 4605
SmaI CCC'GGG
Cuts at: 1872 3664 1872
Size: 1792 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4441 5342 5604 820
Size: 197 926 2498 901 262 1553
Fragments arranged by size:
2498 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4128 493
Size: 3635 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6337
SphI G_CATG'C
Cuts at: 1132 3695 1132
Size: 2563 3774
SspI AAT'ATT
Cuts at: 5 52 4261 5
Size: 47 4209 2081
Fragments arranged by size:
4209 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6337
StyI C'CwwG_G
Cuts at: 513 1065 2087 2235 2349 2385 3776 513
Size: 552 1022 148 114 36 1391 3074
Fragments arranged by size:
3074 1391 1022 552 148 114 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3453 4364 4381 4534 4719 5164 325
Size: 1255 1873 911 17 153 185 445 1498
Fragments arranged by size:
1873 1498 1255 911 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4583 6295 96
Size: 274 80 33 51 1443 2606 1712 138
Fragments arranged by size:
2606 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2565 2597 6297 6329 2565
Size: 32 3700 32 2573
Fragments arranged by size:
3700 2573 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3014 1316
Size: 1698 4639
XbaI T'CTAG_A
Cuts at: 3673 3673
Size: 6337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2162 2162
Size: 6337
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6337
XmaI C'CCGG_G
Cuts at: 1870 3662 1870
Size: 1792 4545
XmnI GAAnn'nnTTC
Cuts at: 2514 3044 3071 3236 3320 3485 4466 2514
Size: 530 27 165 84 165 981 4385
Fragments arranged by size:
4385 981 530 165 165 84 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4145 276
Size: 53 83 186 936 2611 2468
Fragments arranged by size:
2611 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI