Restriction map for
pFN21AB7842
(Circular) (Six-base) MAPSORT of: pFN21AB7842.seq Check: 5876 from: 1 to: 6556
pFN21AB7842 6556 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4366 278
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3899 1937
Size: 1962 4594
Acc65I G'GTAC_C
Cuts at: 3877 3877
Size: 6556
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2076 2176 2879 3196 4619 6003 6271 1632
Size: 444 100 703 317 1423 1384 268 1917
Fragments arranged by size:
1917 1423 1384 703 444 317 268 100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6359
AflIII A'CryG_T
Cuts at: 1203 2600 3029 5455 6432 1203
Size: 1397 429 2426 977 1327
Fragments arranged by size:
2426 1397 1327 977 429
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 3423 1166
Size: 2257 4299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6556
AlwNI CAG_nnn'CTG
Cuts at: 4299 5871 4299
Size: 1572 4984
ApaLI G'TGCA_C
Cuts at: 4613 5769 4613
Size: 1156 5400
ApoI r'AATT_y
Cuts at: 1493 2048 2299 2705 3304 3641 3806 3865 4091
Size: 555 251 406 599 337 165 59 226
Cuts at: 4091 4243 6352 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 599 555 406 337 251 226
165 152 59
AseI AT'TA_AT
Cuts at: 160 1032 5110 160
Size: 872 4078 1606
Fragments arranged by size:
4078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 3881 1870
Size: 73 1938 4545
Fragments arranged by size:
4545 1938 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3622 3655 3622
Size: 33 6523
BamHI G'GATC_C
Cuts at: 3886 4253 3886
Size: 367 6189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3877 5332 618
Size: 325 217 627 2090 1455 1842
Fragments arranged by size:
2090 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2720 3875 1391
Size: 1329 1155 4072
Fragments arranged by size:
4072 1329 1155
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2110 2282 3212 928
Size: 971 211 172 930 4272
Fragments arranged by size:
4272 971 930 211 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4747 4781 1903
Size: 34 2810 34 3678
Fragments arranged by size:
3678 2810 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5658 4448
Size: 1210 5346
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5167 136
Size: 107 122 71 631 605 3495 1525
Fragments arranged by size:
3495 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3795 6551 3795
Size: 2756 3800
BlpI GC'TnA_GC
Cuts at: 3973 3973
Size: 6556
Bme1580I G_kGCm'C
Cuts at: 1790 4617 5773 1790
Size: 2827 1156 2573
Fragments arranged by size:
2827 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2928 4287 5245
Size: 416 53 83 399 24 1511 1359 958
Cuts at: 5245 442
Size: 1753
Fragments arranged by size:
1753 1511 1359 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6556
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3567 3817 3567
Size: 250 6306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2772 3612 3801 5216 1239
Size: 1533 840 189 1415 2579
Fragments arranged by size:
2579 1533 1415 840 189
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3419 4681 5546 5844 3419
Size: 1262 865 298 4131
Fragments arranged by size:
4131 1262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2415 3424 5219 882
Size: 617 916 1009 1795 2219
Fragments arranged by size:
2219 1795 1009 916 617
BsaAI yAC'GTr
Cuts at: 493 1204 2497 4347 493
Size: 711 1293 1850 2702
Fragments arranged by size:
2702 1850 1293 711
BsaBI GATnn'nnATC
Cuts at: 2565 6550 2565
Size: 3985 2571
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4363 4745 5380 275
Size: 53 83 186 936 2830 382 635 1451
Fragments arranged by size:
2830 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4989 5387 5661 5808 6219 4989
Size: 398 274 147 411 5326
Fragments arranged by size:
5326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6331
Fragments arranged by size:
6331 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2144 3377 1186
Size: 955 3 1233 4365
Fragments arranged by size:
4365 1233 955 3
BseYI C'CCAG_C
Cuts at: 2134 2635 3468 3720 5759 6255 2134
Size: 501 833 252 2039 496 2435
Fragments arranged by size:
2435 2039 833 501 496 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5983
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4006 4767 5795 1540
Size: 275 186 2005 761 1028 2301
Fragments arranged by size:
2301 2005 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2720 4617 4702 5773 2720
Size: 1897 85 1071 3503
Fragments arranged by size:
3503 1897 1071 85
BsmI GAATG_Cn'
Cuts at: 2986 4067 4160 2986
Size: 1081 93 5382
Fragments arranged by size:
5382 1081 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2720 3875 4617 4702 5773 1391
Size: 399 930 1155 742 85 1071 2174
Fragments arranged by size:
2174 1155 1071 930 742 399 85
BspEI T'CCGG_A
Cuts at: 6219 6219
Size: 6556
BspHI T'CATG_A
Cuts at: 2881 4445 6175 2881
Size: 1564 1730 3262
Fragments arranged by size:
3262 1730 1564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2208 3911 844
Size: 1364 1703 3489
Fragments arranged by size:
3489 1703 1364
BsrBI CCG'CTC
Cuts at: 1394 1823 4443 1394
Size: 429 2620 3507
Fragments arranged by size:
3507 2620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2783 2818 3119 5051 5225 65
Size: 1183 1535 35 301 1932 174 1396
Fragments arranged by size:
1932 1535 1396 1183 301 174 35
BsrFI r'CCGG_y
Cuts at: 1700 1810 5200 5387 1700
Size: 110 3390 187 2869
Fragments arranged by size:
3390 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6556
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6556
BssSI C'ACGA_G
Cuts at: 4616 5628 4616
Size: 1012 5544
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6556
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6556
BstYI r'GATC_y
Cuts at: 1972 2313 3329 3394 3795 3886 4253 4638 4655
Size: 341 1016 65 401 91 367 385 17
Cuts at: 4655 6107 6193 6205 6551 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1016 401 385 367 346 341
91 86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2991 3075 513
Size: 552 1926 84 3994
Fragments arranged by size:
3994 1926 552 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5817
Fragments arranged by size:
5817 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 3623 4072 4866 4886 2097
Size: 1526 449 794 20 3767
Fragments arranged by size:
3767 1526 794 449 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6521
DraI TTT'AAA
Cuts at: 3860 3860
Size: 6556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5563 809
Size: 507 4247 1802
Fragments arranged by size:
4247 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4003 4892 6521
Size: 54 1425 50 275 18 2173 889 1629
Cuts at: 6521 8
Size: 43
Fragments arranged by size:
2173 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4003 1537
Size: 275 2191 4090
Fragments arranged by size:
4090 2191 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 3084 3525 3786 4486 1353
Size: 999 732 441 261 700 3423
Fragments arranged by size:
3423 999 732 700 441 261
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5143 5517 5663 387
Size: 1064 138 3554 374 146 1280
Fragments arranged by size:
3554 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2718 2718
Size: 6556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2176 2772 2879 3196 3612 3801
Size: 393 444 100 596 107 317 416 189
Cuts at: 3801 4619 5216 6003 6271 1239
Size: 818 597 787 268 1524
Fragments arranged by size:
1524 818 787 597 596 444 416 393
317 268 189 107 100
EcoRI G'AATT_C
Cuts at: 2299 3806 3865 4243 2299
Size: 1507 59 378 4612
Fragments arranged by size:
4612 1507 378 59
FspI TGC'GCA
Cuts at: 5062 5062
Size: 6556
HaeII r_GCGC'y
Cuts at: 1678 5703 1678
Size: 4025 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2298 2330 2707 2739 3379
Size: 32 135 32 726 32 377 32 640
Cuts at: 3379 3411 5197 5229 5271 5303 1373
Size: 32 1786 32 42 32 2626
Fragments arranged by size:
2626 1786 726 640 377 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3900 4146 669
Size: 988 281 1962 246 3079
Fragments arranged by size:
3079 1962 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2620 748
Size: 1872 4684
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KpnI G_GTAC'C
Cuts at: 3881 3881
Size: 6556
MfeI C'AATT_G
Cuts at: 4155 4155
Size: 6556
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3372 5670 5854 1305
Size: 2067 2298 184 2007
Fragments arranged by size:
2298 2067 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4734
Fragments arranged by size:
4734 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2601 2935 3019 3103 3423 4514 4873
Size: 648 1435 334 84 84 320 1091 359
Cuts at: 4873 5032 518
Size: 159 2042
Fragments arranged by size:
2042 1435 1091 648 359 334 320 159
84 84
MspA1I CmG'CkG
Cuts at: 1606 3972 4649 5797 6042 1606
Size: 2366 677 1148 245 2120
Fragments arranged by size:
2366 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2991 3075 513
Size: 552 1926 84 3994
Fragments arranged by size:
3994 1926 552 84
NdeI CA'TA_TG
Cuts at: 387 2664 387
Size: 2277 4279
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6556
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 2604 3033 3585 3658 3914 5459 1132
Size: 97 1375 429 552 73 256 1545 2229
Fragments arranged by size:
2229 1545 1375 552 429 256 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6556
PciI A'CATG_T
Cuts at: 2600 3029 5455 2600
Size: 429 2426 3701
Fragments arranged by size:
3701 2426 429
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6556
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PmlI CAC'GTG
Cuts at: 2497 2497
Size: 6556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2189 2221 4625 4657 6138 6170 2189
Size: 32 2404 32 1481 32 2575
Fragments arranged by size:
2575 2404 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6556
PsiI TTA'TAA
Cuts at: 2850 4126 2850
Size: 1276 5280
PstI C_TGCA'G
Cuts at: 830 3908 830
Size: 3078 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
SacI G_AGCT'C
Cuts at: 2720 2720
Size: 6556
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SbfI CC_TGCA'GG
Cuts at: 3908 3908
Size: 6556
ScaI AGT'ACT
Cuts at: 4804 6516 4804
Size: 1712 4844
SexAI A'CCwGG_T
Cuts at: 2405 2405
Size: 6556
SfcI C'TryA_G
Cuts at: 826 1045 3904 5039 5720 5911 826
Size: 219 2859 1135 681 191 1471
Fragments arranged by size:
2859 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6556
SmaI CCC'GGG
Cuts at: 1872 3883 1872
Size: 2011 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3398 4660 5561 5823 820
Size: 197 926 1455 1262 901 262 1553
Fragments arranged by size:
1553 1455 1262 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6556
SphI G_CATG'C
Cuts at: 1132 3914 1132
Size: 2782 3774
SspI AAT'ATT
Cuts at: 5 52 4480 5
Size: 47 4428 2081
Fragments arranged by size:
4428 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6556
StyI C'CwwG_G
Cuts at: 513 1065 2991 3075 3995 513
Size: 552 1926 84 920 3074
Fragments arranged by size:
3074 1926 920 552 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4583 4600 4753 4938 5383 325
Size: 1255 3003 17 153 185 445 1498
Fragments arranged by size:
3003 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 4802 6514
Size: 274 80 33 51 1443 1580 1245 1712
Cuts at: 6514 96
Size: 138
Fragments arranged by size:
1712 1580 1443 1245 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6516 6548 6516
Size: 32 6524
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6556
XbaI T'CTAG_A
Cuts at: 3892 3892
Size: 6556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2358 2358
Size: 6556
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6556
XmaI C'CCGG_G
Cuts at: 1870 3881 1870
Size: 2011 4545
XmnI GAAnn'nnTTC
Cuts at: 2028 2762 2789 2873 2930 3209 4685 2028
Size: 734 27 84 57 279 1476 3899
Fragments arranged by size:
3899 1476 734 279 84 57 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4364 276
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI