Restriction map for
pFN21AB7841
(Circular) (Six-base) MAPSORT of: pFN21AB7841.seq Check: 8713 from: 1 to: 6217
pFN21AB7841 6217 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2979 3179 4280 5664 5932 1632
Size: 1347 200 1101 1384 268 1917
Fragments arranged by size:
1917 1384 1347 1101 268 200
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 3401 5048 3401
Size: 1647 4570
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 2601 1166
Size: 1435 4782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3239 3242 3261 1184
Size: 2055 3 19 4140
Fragments arranged by size:
4140 2055 19 3
AlwNI CAG_nnn'CTG
Cuts at: 2092 3960 5532 2092
Size: 1868 1572 2777
Fragments arranged by size:
2777 1868 1572
ApaLI G'TGCA_C
Cuts at: 2051 4274 5430 2051
Size: 2223 1156 2838
Fragments arranged by size:
2838 2223 1156
ApoI r'AATT_y
Cuts at: 1493 2283 2516 2579 2985 3117 3138 3439 3526
Size: 790 233 63 406 132 21 301 87
Cuts at: 3526 3752 3904 6013 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 790 406 301 233 226 152
132 87 63 21
AseI AT'TA_AT
Cuts at: 160 1032 3375 4771 160
Size: 872 2343 1396 1606
Fragments arranged by size:
2343 1606 1396 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 3542 1870
Size: 73 131 1468 4545
Fragments arranged by size:
4545 1468 131 73
BamHI G'GATC_C
Cuts at: 2625 3547 3914 2625
Size: 922 367 4928
Fragments arranged by size:
4928 922 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 3077 3277 3538 4993
Size: 325 217 627 309 981 200 261 1455
Cuts at: 4993 618
Size: 1842
Fragments arranged by size:
1842 1455 981 627 325 309 261 217
200
BanII G_rGCy'C
Cuts at: 1391 3536 1391
Size: 2145 4072
BbeI G_GCGC'C
Cuts at: 2100 2100
Size: 6217
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 3424 1548
Size: 1876 4341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2076 4828 136
Size: 107 122 71 631 605 404 2752 1525
Fragments arranged by size:
2752 1525 631 605 404 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 3634 3634
Size: 6217
Bme1580I G_kGCm'C
Cuts at: 1790 2055 2619 3280 4278 5434 1790
Size: 265 564 661 998 1156 2573
Fragments arranged by size:
2573 1156 998 661 564 265
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2102 3948 4906
Size: 416 53 83 399 24 685 1846 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
1846 1753 958 685 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2691 1056
Size: 1635 4582
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2787 2787
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4877 1239
Size: 3638 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 4342 5207 5505 2003
Size: 2339 865 298 2715
Fragments arranged by size:
2715 2339 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2097 4024 4406
Size: 53 83 186 936 531 33 1927 382
Cuts at: 4406 5041 275
Size: 635 1451
Fragments arranged by size:
1927 1451 936 635 531 382 186 83
53 33
BsaWI w'CCGG_w
Cuts at: 3401 4650 5048 5322 5469 5880 3401
Size: 1249 398 274 147 411 3738
Fragments arranged by size:
3738 1249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5992
Fragments arranged by size:
5992 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6217
BseYI C'CCAG_C
Cuts at: 2530 2875 5420 5916 2530
Size: 345 2545 496 2831
Fragments arranged by size:
2831 2545 496 345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2742 3147 1163
Size: 573 1006 405 4233
Fragments arranged by size:
4233 1006 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2019 2055 2187 2909 4278 4363 5434 2019
Size: 36 132 722 1369 85 1071 2802
Fragments arranged by size:
2802 1369 1071 722 132 85 36
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2055 2187 2619 2909 3280 3536
Size: 399 229 36 132 432 290 371 256
Cuts at: 3536 4278 4363 5434 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 371 290 256
229 132 85 36
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 2773 4106 5836 2773
Size: 1333 1730 3154
Fragments arranged by size:
3154 1730 1333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3572 844
Size: 2728 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4104 1394
Size: 429 2281 3507
Fragments arranged by size:
3507 2281 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2129 3026 4712 4886 65
Size: 1183 881 897 1686 174 1396
Fragments arranged by size:
1686 1396 1183 897 881 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2099 3401 4861 5048 1700
Size: 110 289 1302 1460 187 2869
Fragments arranged by size:
2869 1460 1302 289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3025 3254 794
Size: 2231 229 3757
Fragments arranged by size:
3757 2231 229
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 2625 3547 3914 4299 4316 5768 5854 5866
Size: 653 922 367 385 17 1452 86 12
Cuts at: 5866 6212 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 922 653 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2588 2831 513
Size: 552 939 584 243 3899
Fragments arranged by size:
3899 939 584 552 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2563 2920 3259 3277 3733 4527 4547 2563
Size: 357 339 18 456 794 20 4233
Fragments arranged by size:
4233 794 456 357 339 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2448 2483 529
Size: 35 1884 35 4263
Fragments arranged by size:
4263 1884 35 35
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DraIII CAC_nnn'GTG
Cuts at: 2823 2823
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3664 4553 6182
Size: 54 1425 50 275 18 1834 889 1629
Cuts at: 6182 8
Size: 43
Fragments arranged by size:
1834 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2756 3049 4147 1353
Size: 1403 293 1098 3423
Fragments arranged by size:
3423 1403 1098 293
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4804 5178 5324 387
Size: 1064 138 3215 374 146 1280
Fragments arranged by size:
3215 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2979 3179 4280 4877 5664 5932 1239
Size: 393 1347 200 1101 597 787 268 1524
Fragments arranged by size:
1524 1347 1101 787 597 393 268 200
EcoO109I rG'GnC_Cy
Cuts at: 2527 3395 2527
Size: 868 5349
EcoRI G'AATT_C
Cuts at: 3138 3526 3904 3138
Size: 388 378 5451
Fragments arranged by size:
5451 388 378
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2100 2106 2882 5364 1678
Size: 422 6 776 2482 2531
Fragments arranged by size:
2531 2482 776 422 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4858 4890 4932 4964 1373
Size: 32 135 32 3286 32 42 32 2626
Fragments arranged by size:
3286 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2635 3561 3807 669
Size: 988 281 697 926 246 3079
Fragments arranged by size:
3079 988 926 697 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KasI G'GCGC_C
Cuts at: 2096 2096
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5331 5515 1305
Size: 4026 184 2007
Fragments arranged by size:
4026 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4395
Fragments arranged by size:
4395 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2601 3019 4175 4534 4693 518
Size: 648 1435 418 1156 359 159 2042
Fragments arranged by size:
2042 1435 1156 648 418 359 159
MspA1I CmG'CkG
Cuts at: 1606 3633 4310 5458 5703 1606
Size: 2027 677 1148 245 2120
Fragments arranged by size:
2120 2027 1148 677 245
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 6217
NarI GG'CG_CC
Cuts at: 2097 2097
Size: 6217
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4726
Fragments arranged by size:
4726 939 552
NdeI CA'TA_TG
Cuts at: 387 2278 387
Size: 1891 4326
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 2687 1052
Size: 1635 4582
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 3575 5120 1132
Size: 97 2346 1545 2229
Fragments arranged by size:
2346 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2171 1108
Size: 902 161 5154
Fragments arranged by size:
5154 902 161
PfoI T'CCnGG_A
Cuts at: 3006 3006
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2527 3395 2527
Size: 868 5349
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PstI C_TGCA'G
Cuts at: 830 3569 830
Size: 2739 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SanDI GG'GwC_CC
Cuts at: 2527 3395 2527
Size: 868 5349
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 3565 4700 5381 5572 826
Size: 219 2520 1135 681 191 1471
Fragments arranged by size:
2520 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2098 2098
Size: 6217
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 2099 1700
Size: 399 5818
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2018 4321 5222 5484 820
Size: 197 926 75 2303 901 262 1553
Fragments arranged by size:
2303 1553 926 901 262 197 75
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6217
StyI C'CwwG_G
Cuts at: 513 1065 2004 2266 3656 513
Size: 552 939 262 1390 3074
Fragments arranged by size:
3074 1390 939 552 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2002 2202 2300 2760 4244 4261 4414
Size: 1255 422 200 98 460 1484 17 153
Cuts at: 4414 4599 5044 325
Size: 185 445 1498
Fragments arranged by size:
1498 1484 1255 460 445 422 200 185
153 98 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4463 6175 96
Size: 274 80 33 51 1443 2486 1712 138
Fragments arranged by size:
2486 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2296 2328 6177 6209 2296
Size: 32 3849 32 2304
Fragments arranged by size:
3849 2304 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3393 1316
Size: 2077 4140
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2164 2164
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 2985 4346 2985
Size: 1361 4856
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SapI SexAI SfiI SrfI SwaI