Restriction map for
pFN21AB7840
(Circular) (Six-base) MAPSORT of: pFN21AB7840.seq Check: 526 from: 1 to: 6550
pFN21AB7840 6550 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 4360 278
Size: 53 83 186 936 2056 768 2468
Fragments arranged by size:
2468 2056 936 768 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2715 3893 1937
Size: 778 1178 4594
Fragments arranged by size:
4594 1178 778
Acc65I G'GTAC_C
Cuts at: 3871 3871
Size: 6550
AclI AA'CG_TT
Cuts at: 1563 4677 5050 1563
Size: 3114 373 3063
Fragments arranged by size:
3114 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3471 4613 5997 6265 1632
Size: 1839 1142 1384 268 1917
Fragments arranged by size:
1917 1839 1384 1142 268
AfeI AGC'GCT
Cuts at: 2870 2870
Size: 6550
AflII C'TTAA_G
Cuts at: 820 1017 2303 820
Size: 197 1286 5067
Fragments arranged by size:
5067 1286 197
AflIII A'CryG_T
Cuts at: 1203 3421 5449 6426 1203
Size: 2218 2028 977 1327
Fragments arranged by size:
2218 2028 1327 977
AgeI A'CCGG_T
Cuts at: 5381 5381
Size: 6550
AhdI GACnn_n'nnGTC
Cuts at: 5279 5279
Size: 6550
AleI CACnn'nnGTG
Cuts at: 1166 2424 1166
Size: 1258 5292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2455 3018 3728 3752 1184
Size: 1271 563 710 24 3982
Fragments arranged by size:
3982 1271 710 563 24
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3315 3347 3315
Size: 32 6518
AlwNI CAG_nnn'CTG
Cuts at: 2401 2674 3269 4293 5865 2401
Size: 273 595 1024 1572 3086
Fragments arranged by size:
3086 1572 1024 595 273
ApaI G_GGCC'C
Cuts at: 3046 3526 3046
Size: 480 6070
ApaLI G'TGCA_C
Cuts at: 2177 2791 3477 4607 5763 2177
Size: 614 686 1130 1156 2964
Fragments arranged by size:
2964 1156 1130 686 614
ApoI r'AATT_y
Cuts at: 1493 3859 4085 4237 6346 1493
Size: 2366 226 152 2109 1697
Fragments arranged by size:
2366 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5104 160
Size: 872 4072 1606
Fragments arranged by size:
4072 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6550
AvaI C'yCGr_G
Cuts at: 1870 1943 3093 3344 3825 3875 1870
Size: 73 1150 251 481 50 4545
Fragments arranged by size:
4545 1150 481 251 73 50
AvrII C'CTAG_G
Cuts at: 3694 3694
Size: 6550
BamHI G'GATC_C
Cuts at: 3880 4247 3880
Size: 367 6183
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2028 2185 2290 2627 2637
Size: 325 217 627 241 157 105 337 10
Cuts at: 2637 3236 3871 5326 618
Size: 599 635 1455 1842
Fragments arranged by size:
1842 1455 635 627 599 337 325 241
217 157 105 10
BanII G_rGCy'C
Cuts at: 1391 2475 3046 3120 3279 3301 3333 3526 3869
Size: 1084 571 74 159 22 32 193 343
Cuts at: 3869 1391
Size: 4072
Fragments arranged by size:
4072 1084 571 343 193 159 74 32
22
BbeI G_GCGC'C
Cuts at: 2032 2032
Size: 6550
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5579
BbvCI CC'TCA_GC
Cuts at: 2802 2802
Size: 6550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4741 4775 1903
Size: 34 2804 34 3678
Fragments arranged by size:
3678 2804 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4442 5652 4442
Size: 1210 5340
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6550
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2619 5161 136
Size: 107 122 71 631 605 947 2542 1525
Fragments arranged by size:
2542 1525 947 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2136 6545 2136
Size: 4409 2141
BlpI GC'TnA_GC
Cuts at: 3967 3967
Size: 6550
Bme1580I G_kGCm'C
Cuts at: 1790 2108 2181 2295 2795 3046 3481 3526 4611
Size: 318 73 114 500 251 435 45 1085
Cuts at: 4611 5767 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1085 500 435 318 251 114
73 45
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6550
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2559 3335 4281
Size: 416 53 83 399 24 1142 776 946
Cuts at: 4281 5239 442
Size: 958 1753
Fragments arranged by size:
1753 1142 958 946 776 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6550
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3295 3315 3295
Size: 20 6530
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 2149 3111 3169 3625 3637 5210 1239
Size: 795 115 962 58 456 12 1573 2579
Fragments arranged by size:
2579 1573 962 795 456 115 58 12
Bpu10I CC'TnA_GC
Cuts at: 2802 3744 2802
Size: 942 5608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4675 5540 5838 4675
Size: 865 298 5387
Fragments arranged by size:
5387 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3215 3755 5213 882
Size: 617 1716 540 1458 2219
Fragments arranged by size:
2219 1716 1458 617 540
BsaAI yAC'GTr
Cuts at: 493 1204 2664 4341 493
Size: 711 1460 1677 2702
Fragments arranged by size:
2702 1677 1460 711
BsaBI GATnn'nnATC
Cuts at: 6544 6544
Size: 6550
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2029 2725 3589 4357
Size: 53 83 186 936 496 696 864 768
Cuts at: 4357 4739 5374 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 864 768 696 635 496 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4983 5381 5655 5802 6213 4983
Size: 398 274 147 411 5320
Fragments arranged by size:
5320 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 3296 3315 3326
Size: 30 165 30 828 30 1035 19 11
Cuts at: 3326 3345 1178
Size: 19 4383
Fragments arranged by size:
4383 1035 828 165 30 30 30 19
19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2291 2471 2579 3287 1186
Size: 994 111 180 108 708 4449
Fragments arranged by size:
4449 994 708 180 111 108
BseYI C'CCAG_C
Cuts at: 3049 3489 3527 5753 6249 3049
Size: 440 38 2226 496 3350
Fragments arranged by size:
3350 2226 496 440 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 2425 2637 3748 1163
Size: 573 651 38 212 1111 3965
Fragments arranged by size:
3965 1111 651 573 212 38
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4000 4761 5789 1540
Size: 275 186 1405 594 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 594 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 2475 2795 2955 3416 3481 4611 4696 5767
Size: 294 320 160 461 65 1130 85 1071
Cuts at: 5767 2181
Size: 2964
Fragments arranged by size:
2964 1130 1071 461 320 294 160 85
65
BsmI GAATG_Cn'
Cuts at: 2744 4061 4154 2744
Size: 1317 93 5140
Fragments arranged by size:
5140 1317 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2181 2295 2475 2795 2955 3046
Size: 399 318 73 114 180 320 160 91
Cuts at: 3046 3120 3279 3301 3333 3416 3481 3526 3869
Size: 74 159 22 32 83 65 45 343
Cuts at: 3869 4611 4696 5767 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 343 320 318 180
160 159 114 91 85 83 74 73
65 45 32 22
BspEI T'CCGG_A
Cuts at: 6213 6213
Size: 6550
BspHI T'CATG_A
Cuts at: 4439 6169 4439
Size: 1730 4820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2305 3387 3905 844
Size: 1461 1082 518 3489
Fragments arranged by size:
3489 1461 1082 518
BsrBI CCG'CTC
Cuts at: 1394 1823 2034 4437 1394
Size: 429 211 2403 3507
Fragments arranged by size:
3507 2403 429 211
BsrDI GCAATG_nn'
Cuts at: 65 1248 2146 3762 5045 5219 65
Size: 1183 898 1616 1283 174 1396
Fragments arranged by size:
1616 1396 1283 1183 898 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3155 5194 5381 1700
Size: 110 1345 2039 187 2869
Fragments arranged by size:
2869 2039 1345 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6550
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6550
BssSI C'ACGA_G
Cuts at: 4610 5622 4610
Size: 1012 5538
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2176 2185 2401 2410 2606 794
Size: 1382 9 216 9 196 4738
Fragments arranged by size:
4738 1382 216 196 9 9
BstBI TT'CG_AA
Cuts at: 5354 5354
Size: 6550
BstEII G'GTnAC_C
Cuts at: 1184 2539 1184
Size: 1355 5195
BstYI r'GATC_y
Cuts at: 1972 2136 3880 4247 4632 4649 6101 6187 6199
Size: 164 1744 367 385 17 1452 86 12
Cuts at: 6199 6545 1972
Size: 346 1977
Fragments arranged by size:
1977 1744 1452 385 367 346 164 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3270 3270
Size: 6550
BtgI C'CryG_G
Cuts at: 513 1065 2122 2159 513
Size: 552 1057 37 4904
Fragments arranged by size:
4904 1057 552 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2581 487
Size: 683 56 1355 4456
Fragments arranged by size:
4456 1355 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 2181 2607 2896 3343 3750 4066 4860 4880
Size: 73 426 289 447 407 316 794 20
Cuts at: 4880 2108
Size: 3778
Fragments arranged by size:
3778 794 447 426 407 316 289 73
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6550
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6515
DraI TTT'AAA
Cuts at: 3854 3854
Size: 6550
DraIII CAC_nnn'GTG
Cuts at: 2185 2185
Size: 6550
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5557 809
Size: 507 4241 1802
Fragments arranged by size:
4241 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3367 3997 4886
Size: 54 1425 50 275 18 1537 630 889
Cuts at: 4886 6515 8
Size: 1629 43
Fragments arranged by size:
1629 1537 1425 889 630 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3997 1537
Size: 275 2185 4090
Fragments arranged by size:
4090 2185 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2448 4480 1353
Size: 1095 2032 3423
Fragments arranged by size:
3423 2032 1095
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5137 5511 5657 387
Size: 1064 138 3548 374 146 1280
Fragments arranged by size:
3548 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2473 2473
Size: 6550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2149 3111 3169 3471 3625 3637
Size: 393 402 115 962 58 302 154 12
Cuts at: 3637 4613 5210 5997 6265 1239
Size: 976 597 787 268 1524
Fragments arranged by size:
1524 976 962 787 597 402 393 302
268 154 115 58 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2342 2342
Size: 6550
EcoO109I rG'GnC_Cy
Cuts at: 2019 2062 2353 3043 3266 3522 3523 2019
Size: 43 291 690 223 256 1 5046
Fragments arranged by size:
5046 690 291 256 223 43 1
EcoRI G'AATT_C
Cuts at: 3859 4237 3859
Size: 378 6172
FspI TGC'GCA
Cuts at: 2528 5056 2528
Size: 2528 4022
FspAI rTGC'GCAy
Cuts at: 2528 2528
Size: 6550
HaeII r_GCGC'y
Cuts at: 1678 2032 2239 2872 3257 3496 5697 1678
Size: 354 207 633 385 239 2201 2531
Fragments arranged by size:
2531 2201 633 385 354 239 207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 3066 3098 3599
Size: 32 135 32 671 32 791 32 501
Cuts at: 3599 3617 3631 3649 3690 3722 3802 3834 5191
Size: 18 14 18 41 32 80 32 1357
Cuts at: 5191 5223 5265 5297 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1357 791 671 501 135 80 42
41 32 32 32 32 32 32 32
32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2365 2683 3894 4140 669
Size: 988 281 427 318 1211 246 3079
Fragments arranged by size:
3079 1211 988 427 318 281 246
HindIII A'AGCT_T
Cuts at: 748 2850 748
Size: 2102 4448
HpaI GTT'AAC
Cuts at: 4140 4140
Size: 6550
KasI G'GCGC_C
Cuts at: 2028 2028
Size: 6550
KpnI G_GTAC'C
Cuts at: 3875 3875
Size: 6550
MfeI C'AATT_G
Cuts at: 4149 4149
Size: 6550
MluI A'CGCG_T
Cuts at: 3421 6426 3421
Size: 3005 3545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 5664 5848 1305
Size: 969 3390 184 2007
Fragments arranged by size:
3390 2007 969 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4728
Fragments arranged by size:
4728 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2424 2600 3000 3755 4508 4867 5026
Size: 648 1258 176 400 755 753 359 159
Cuts at: 5026 518
Size: 2042
Fragments arranged by size:
2042 1258 755 753 648 400 359 176
159
MspA1I CmG'CkG
Cuts at: 1606 2161 2334 2398 2410 2494 3015 3147 3966
Size: 555 173 64 12 84 521 132 819
Cuts at: 3966 4643 5791 6036 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 819 677 555 521 245 173
132 84 64 12
NaeI GCC'GGC
Cuts at: 3157 3157
Size: 6550
NarI GG'CG_CC
Cuts at: 2029 2029
Size: 6550
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5998
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6550
NgoMIV G'CCGG_C
Cuts at: 3155 3155
Size: 6550
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6550
NotI GC'GGCC_GC
Cuts at: 3997 3997
Size: 6550
NspI r_CATG'y
Cuts at: 1132 1229 2174 3005 3908 5453 1132
Size: 97 945 831 903 1545 2229
Fragments arranged by size:
2229 1545 945 903 831 97
PasI CC'CwG_GG
Cuts at: 1348 3519 3792 1348
Size: 2171 273 4106
Fragments arranged by size:
4106 2171 273
PciI A'CATG_T
Cuts at: 5449 5449
Size: 6550
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6550
PfoI T'CCnGG_A
Cuts at: 2021 2021
Size: 6550
PmeI GTTT'AAAC
Cuts at: 3854 3854
Size: 6550
PmlI CAC'GTG
Cuts at: 2664 2664
Size: 6550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3315 3347 4619 4651 6132 6164 3315
Size: 32 1272 32 1481 32 3701
Fragments arranged by size:
3701 1481 1272 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2019 2062 2353 2019
Size: 43 291 6216
Fragments arranged by size:
6216 291 43
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6550
PsiI TTA'TAA
Cuts at: 4120 4120
Size: 6550
PspOMI G'GGCC_C
Cuts at: 3042 3522 3042
Size: 480 6070
PstI C_TGCA'G
Cuts at: 830 3104 3902 830
Size: 2274 798 3478
Fragments arranged by size:
3478 2274 798
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6550
PvuII CAG'CTG
Cuts at: 2334 2398 2410 3147 2334
Size: 64 12 737 5737
Fragments arranged by size:
5737 737 64 12
SacI G_AGCT'C
Cuts at: 2475 2475
Size: 6550
SacII CC_GC'GG
Cuts at: 2162 2162
Size: 6550
SalI G'TCGA_C
Cuts at: 1936 3892 1936
Size: 1956 4594
SanDI GG'GwC_CC
Cuts at: 2019 2019
Size: 6550
SbfI CC_TGCA'GG
Cuts at: 3902 3902
Size: 6550
ScaI AGT'ACT
Cuts at: 2438 2933 4798 6510 2438
Size: 495 1865 1712 2478
Fragments arranged by size:
2478 1865 1712 495
SfcI C'TryA_G
Cuts at: 826 1045 2092 3025 3100 3261 3898 5033 5714
Size: 219 1047 933 75 161 637 1135 681
Cuts at: 5714 5905 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1047 933 681 637 219 191
161 75
SfoI GGC'GCC
Cuts at: 2030 2030
Size: 6550
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6550
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6550
SmaI CCC'GGG
Cuts at: 1872 3877 1872
Size: 2005 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 3093 4654 5555 5817 820
Size: 197 926 360 790 1561 901 262 1553
Fragments arranged by size:
1561 1553 926 901 790 360 262 197
SnaBI TAC'GTA
Cuts at: 493 4341 493
Size: 3848 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6550
SphI G_CATG'C
Cuts at: 1132 2174 3005 3908 1132
Size: 1042 831 903 3774
Fragments arranged by size:
3774 1042 903 831
SspI AAT'ATT
Cuts at: 5 52 4474 5
Size: 47 4422 2081
Fragments arranged by size:
4422 2081 47
StuI AGG'CCT
Cuts at: 1517 2301 2622 3078 1517
Size: 784 321 456 4989
Fragments arranged by size:
4989 784 456 321
StyI C'CwwG_G
Cuts at: 513 1065 2968 3106 3694 3989 513
Size: 552 1903 138 588 295 3074
Fragments arranged by size:
3074 1903 588 552 295 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2278 2615 2655 3392 3532 3594 4577
Size: 1255 698 337 40 737 140 62 983
Cuts at: 4577 4594 4747 4932 5377 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 983 737 698 445 337 185
153 140 62 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2436 2931 4796
Size: 274 80 33 51 1443 459 495 1865
Cuts at: 4796 6508 96
Size: 1712 138
Fragments arranged by size:
1865 1712 1443 495 459 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2231 2263 3296 3328 3460 3492 6510 6542 2231
Size: 32 1033 32 132 32 3018 32 2239
Fragments arranged by size:
3018 2239 1033 132 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2351 1316
Size: 1035 5515
XbaI T'CTAG_A
Cuts at: 3886 3886
Size: 6550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3322 3322
Size: 6550
XhoI C'TCGA_G
Cuts at: 1943 3093 1943
Size: 1150 5400
XmaI C'CCGG_G
Cuts at: 1870 3875 1870
Size: 2005 4545
XmnI GAAnn'nnTTC
Cuts at: 4679 4679
Size: 6550
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 4358 276
Size: 53 83 186 936 2056 768 2468
Fragments arranged by size:
2468 2056 936 768 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BfrBI BsiWI BsmBI BstXI BstZ17I
EcoRV FalI FseI NruI NsiI PacI PspXI PsrI
RsrII SapI SexAI SfiI SrfI SwaI