Restriction map for
pFN21AB7838
(Circular) (Six-base) MAPSORT of: pFN21AB7838.seq Check: 4885 from: 1 to: 6547
pFN21AB7838 6547 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3025 3217 3025
Size: 192 6355
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4357 278
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2311 3890 1937
Size: 374 1579 4594
Fragments arranged by size:
4594 1579 374
Acc65I G'GTAC_C
Cuts at: 3868 3868
Size: 6547
AclI AA'CG_TT
Cuts at: 1563 4674 5047 1563
Size: 3111 373 3063
Fragments arranged by size:
3111 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2534 3556 3749 4610 5994 6262 1632
Size: 902 1022 193 861 1384 268 1917
Fragments arranged by size:
1917 1384 1022 902 861 268 193
AflII C'TTAA_G
Cuts at: 820 1017 3419 820
Size: 197 2402 3948
Fragments arranged by size:
3948 2402 197
AflIII A'CryG_T
Cuts at: 1203 3180 3267 5446 6423 1203
Size: 1977 87 2179 977 1327
Fragments arranged by size:
2179 1977 1327 977 87
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 5276 5276
Size: 6547
AleI CACnn'nnGTG
Cuts at: 1166 2436 3179 3629 1166
Size: 1270 743 450 4084
Fragments arranged by size:
4084 1270 743 450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6547
AlwNI CAG_nnn'CTG
Cuts at: 2134 4290 5862 2134
Size: 2156 1572 2819
Fragments arranged by size:
2819 2156 1572
ApaI G_GGCC'C
Cuts at: 2759 2890 2759
Size: 131 6416
ApaLI G'TGCA_C
Cuts at: 2441 4604 5760 2441
Size: 2163 1156 3228
Fragments arranged by size:
3228 2163 1156
ApoI r'AATT_y
Cuts at: 1493 3667 3856 4082 4234 6343 1493
Size: 2174 189 226 152 2109 1697
Fragments arranged by size:
2174 2109 1697 226 189 152
AseI AT'TA_AT
Cuts at: 160 1032 5101 160
Size: 872 4069 1606
Fragments arranged by size:
4069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 2422 2601 2883 3376 3655 3872 1870
Size: 73 479 179 282 493 279 217 4545
Fragments arranged by size:
4545 493 479 282 279 217 179 73
BamHI G'GATC_C
Cuts at: 3877 4244 3877
Size: 367 6180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3159 3205 3428 3530 3868
Size: 325 217 627 1372 46 223 102 338
Cuts at: 3868 5323 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1372 627 338 325 223 217
102 46
BanII G_rGCy'C
Cuts at: 1391 2300 2759 2853 2890 3712 3721 3866 1391
Size: 909 459 94 37 822 9 145 4072
Fragments arranged by size:
4072 909 822 459 145 94 37 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3636 3670 4738 4772 1903
Size: 34 1699 34 1068 34 3678
Fragments arranged by size:
3678 1699 1068 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4439 5649 4439
Size: 1210 5337
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6547
BfrBI ATG'CAT
Cuts at: 2960 3808 2960
Size: 848 5699
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2754 5158 136
Size: 107 122 71 631 605 1082 2404 1525
Fragments arranged by size:
2404 1525 1082 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2898 3306 6542 2898
Size: 408 3236 2903
Fragments arranged by size:
3236 2903 408
BlpI GC'TnA_GC
Cuts at: 3964 3964
Size: 6547
Bme1580I G_kGCm'C
Cuts at: 1790 2445 2711 2759 2890 3162 4608 5764 1790
Size: 655 266 48 131 272 1446 1156 2573
Fragments arranged by size:
2573 1446 1156 655 272 266 131 48
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6547
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2800 4278 5236
Size: 416 53 83 399 24 1383 1478 958
Cuts at: 5236 442
Size: 1753
Fragments arranged by size:
1753 1478 1383 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 3337 3569 2545
Size: 792 232 5523
Fragments arranged by size:
5523 792 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2044 2735 2958 3674 5207 1239
Size: 805 691 223 716 1533 2579
Fragments arranged by size:
2579 1533 805 716 691 223
Bpu10I CC'TnA_GC
Cuts at: 2847 3497 2847
Size: 650 5897
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3758 4672 5537 5835 3758
Size: 914 865 298 4470
Fragments arranged by size:
4470 914 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3540 5210 882
Size: 617 2041 1670 2219
Fragments arranged by size:
2219 2041 1670 617
BsaAI yAC'GTr
Cuts at: 493 1204 3181 4338 493
Size: 711 1977 1157 2702
Fragments arranged by size:
2702 1977 1157 711
BsaBI GATnn'nnATC
Cuts at: 6541 6541
Size: 6547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2825 3840 4354
Size: 53 83 186 936 507 785 1015 514
Cuts at: 4354 4736 5371 275
Size: 382 635 1451
Fragments arranged by size:
1451 1015 936 785 635 514 507 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2776 4980 5378 5652 5799 6210 2776
Size: 2204 398 274 147 411 3113
Fragments arranged by size:
3113 2204 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 3097 3127 1178
Size: 30 165 30 639 30 1025 30 4598
Fragments arranged by size:
4598 1025 639 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2059 2266 2269 3082 3620 3623 1186
Size: 873 207 3 813 538 3 4110
Fragments arranged by size:
4110 873 813 538 207 3 3
BseYI C'CCAG_C
Cuts at: 2222 2348 2730 2923 2994 3048 3613 5750 6246
Size: 126 382 193 71 54 565 2137 496
Cuts at: 6246 2222
Size: 2523
Fragments arranged by size:
2523 2137 565 496 382 193 126 71
54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3108 3320 1163
Size: 573 1372 212 4390
Fragments arranged by size:
4390 1372 573 212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3997 4758 5786 1540
Size: 275 186 1996 761 1028 2301
Fragments arranged by size:
2301 1996 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2360 2445 4608 4693 5764 2360
Size: 85 2163 85 1071 3143
Fragments arranged by size:
3143 2163 1071 85 85
BsmI GAATG_Cn'
Cuts at: 2214 3808 4058 4151 2214
Size: 1594 250 93 4610
Fragments arranged by size:
4610 1594 250 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2598 3689 2598
Size: 1091 5456
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2300 2360 2445 2711 2759 2853 2890
Size: 399 510 60 85 266 48 94 37
Cuts at: 2890 3162 3712 3721 3866 4608 4693 5764 1391
Size: 272 550 9 145 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 550 510 399 272 266
145 94 85 85 60 48 37 9
BspEI T'CCGG_A
Cuts at: 6210 6210
Size: 6547
BspHI T'CATG_A
Cuts at: 4436 6166 4436
Size: 1730 4817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3025 3217 3558 3795 3902 844
Size: 2181 192 341 237 107 3489
Fragments arranged by size:
3489 2181 341 237 192 107
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 4434 1394
Size: 429 1831 780 3507
Fragments arranged by size:
3507 1831 780 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2570 5042 5216 65
Size: 1183 1322 2472 174 1396
Fragments arranged by size:
2472 1396 1322 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3735 5191 5378 1700
Size: 110 1925 1456 187 2869
Fragments arranged by size:
2869 1925 1456 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6547
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6547
BssSI C'ACGA_G
Cuts at: 4607 5619 4607
Size: 1012 5535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2556 2650 2706 794
Size: 1762 94 56 4635
Fragments arranged by size:
4635 1762 94 56
BstBI TT'CG_AA
Cuts at: 5351 5351
Size: 6547
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6547
BstXI CCAn_nnnn'nTGG
Cuts at: 2680 2994 3541 2680
Size: 314 547 5686
Fragments arranged by size:
5686 547 314
BstYI r'GATC_y
Cuts at: 1972 2834 2898 3306 3877 4244 4629 4646 6098
Size: 862 64 408 571 367 385 17 1452
Cuts at: 6098 6184 6196 6542 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 862 571 408 385 367 346
86 64 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2322 2751 3534 3627 513
Size: 552 1257 429 783 93 3433
Fragments arranged by size:
3433 1257 783 552 429 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2975 487
Size: 683 56 1749 4059
Fragments arranged by size:
4059 1749 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4063 4857 4877 3292
Size: 771 794 20 4962
Fragments arranged by size:
4962 794 771 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2017 2052 529
Size: 35 1453 35 5024
Fragments arranged by size:
5024 1453 35 35
DraI TTT'AAA
Cuts at: 3851 3851
Size: 6547
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5554 809
Size: 507 4238 1802
Fragments arranged by size:
4238 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 2682 3994
Size: 54 1425 50 275 18 489 363 1312
Cuts at: 3994 4883 6512 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1312 889 489 363 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3994 1537
Size: 275 2182 4090
Fragments arranged by size:
4090 2182 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3626 3717 4477 1353
Size: 2273 91 760 3423
Fragments arranged by size:
3423 2273 760 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5134 5508 5654 387
Size: 1064 138 3545 374 146 1280
Fragments arranged by size:
3545 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2534 2735 2958 3556 3674 3749
Size: 393 412 490 201 223 598 118 75
Cuts at: 3749 4610 5207 5994 6262 1239
Size: 861 597 787 268 1524
Fragments arranged by size:
1524 861 787 598 597 490 412 393
268 223 201 118 75
EcoNI CCTnn'n_nnAGG
Cuts at: 3419 3419
Size: 6547
EcoO109I rG'GnC_Cy
Cuts at: 2747 2756 3064 2747
Size: 9 308 6230
Fragments arranged by size:
6230 308 9
EcoRI G'AATT_C
Cuts at: 3667 3856 4234 3667
Size: 189 378 5980
Fragments arranged by size:
5980 378 189
EcoRV GAT'ATC
Cuts at: 2871 2871
Size: 6547
FspI TGC'GCA
Cuts at: 5053 5053
Size: 6547
HaeII r_GCGC'y
Cuts at: 1678 3599 5694 1678
Size: 1921 2095 2531
Fragments arranged by size:
2531 2095 1921
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2405 2437 2855
Size: 32 135 32 653 32 148 32 418
Cuts at: 2855 2887 5188 5220 5262 5294 1373
Size: 32 2301 32 42 32 2626
Fragments arranged by size:
2626 2301 653 418 148 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3891 4137 669
Size: 988 281 1953 246 3079
Fragments arranged by size:
3079 1953 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6547
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KpnI G_GTAC'C
Cuts at: 3872 3872
Size: 6547
MfeI C'AATT_G
Cuts at: 4146 4146
Size: 6547
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2046 2456 3171 5661 5845 1305
Size: 741 410 715 2490 184 2007
Fragments arranged by size:
2490 2007 741 715 410 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2321 2684 10
Size: 54 1425 343 489 363 3873
Fragments arranged by size:
3873 1425 489 363 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2436 3179 3183 3539 3629 4505 4864
Size: 648 1270 743 4 356 90 876 359
Cuts at: 4864 5023 518
Size: 159 2042
Fragments arranged by size:
2042 1270 876 743 648 359 356 159
90 4
MspA1I CmG'CkG
Cuts at: 1606 2556 2647 2713 2821 3135 3341 3629 3963
Size: 950 91 66 108 314 206 288 334
Cuts at: 3963 4640 5788 6033 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 950 677 334 314 288 245
206 108 91 66
NcoI C'CATG_G
Cuts at: 513 1065 2322 2751 3534 513
Size: 552 1257 429 783 3526
Fragments arranged by size:
3526 1257 783 552 429
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3994 3994
Size: 6547
NsiI A_TGCA'T
Cuts at: 2962 3810 2962
Size: 848 5699
NspI r_CATG'y
Cuts at: 1132 1229 2599 3258 3271 3808 3905 5450 1132
Size: 97 1370 659 13 537 97 1545 2229
Fragments arranged by size:
2229 1545 1370 659 537 97 97 13
PasI CC'CwG_GG
Cuts at: 1348 2303 1348
Size: 955 5592
PciI A'CATG_T
Cuts at: 3267 5446 3267
Size: 2179 4368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6547
PfoI T'CCnGG_A
Cuts at: 3690 3690
Size: 6547
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PmlI CAC'GTG
Cuts at: 3181 3181
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 6129 6161 4616
Size: 32 1481 32 5002
Fragments arranged by size:
5002 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6547
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PspOMI G'GGCC_C
Cuts at: 2755 2886 2755
Size: 131 6416
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2483 2515 2483
Size: 32 6515
PstI C_TGCA'G
Cuts at: 830 3522 3649 3899 830
Size: 2692 127 250 3478
Fragments arranged by size:
3478 2692 250 127
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6547
PvuII CAG'CTG
Cuts at: 2821 3135 2821
Size: 314 6233
SacII CC_GC'GG
Cuts at: 3630 3630
Size: 6547
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SapI GCTCTTCn'nnn_
Cuts at: 3717 3717
Size: 6547
SbfI CC_TGCA'GG
Cuts at: 3522 3899 3522
Size: 377 6170
ScaI AGT'ACT
Cuts at: 4795 6507 4795
Size: 1712 4835
SfcI C'TryA_G
Cuts at: 826 1045 2101 3511 3518 3645 3895 5030 5711
Size: 219 1056 1410 7 127 250 1135 681
Cuts at: 5711 5902 826
Size: 191 1471
Fragments arranged by size:
1471 1410 1135 1056 681 250 219 191
127 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 2754 2754
Size: 6547
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 2885 3378 3874 1872
Size: 1013 493 496 4545
Fragments arranged by size:
4545 1013 496 493
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2601 3419 3773 4651 5552 5814
Size: 197 926 658 818 354 878 901 262
Cuts at: 5814 820
Size: 1553
Fragments arranged by size:
1553 926 901 878 818 658 354 262
197
SnaBI TAC'GTA
Cuts at: 493 4338 493
Size: 3845 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6547
SphI G_CATG'C
Cuts at: 1132 3905 1132
Size: 2773 3774
SrfI GCCC'GGGC
Cuts at: 2885 2885
Size: 6547
SspI AAT'ATT
Cuts at: 5 52 4471 5
Size: 47 4419 2081
Fragments arranged by size:
4419 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6547
StyI C'CwwG_G
Cuts at: 513 1065 2140 2322 2751 3039 3534 3741 3986
Size: 552 1075 182 429 288 495 207 245
Cuts at: 3986 513
Size: 3074
Fragments arranged by size:
3074 1075 552 495 429 288 245 207
182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2201 2236 2740 2744 3191 4574 4591
Size: 1255 621 35 504 4 447 1383 17
Cuts at: 4591 4744 4929 5374 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1383 1255 621 504 447 445 185
153 35 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2499 4793 6505
Size: 274 80 33 51 1443 522 2294 1712
Cuts at: 6505 96
Size: 138
Fragments arranged by size:
2294 1712 1443 522 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3097 3129 6507 6539 3097
Size: 32 3378 32 3105
Fragments arranged by size:
3378 3105 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3407 1316
Size: 2091 4456
XbaI T'CTAG_A
Cuts at: 3883 3883
Size: 6547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6547
XhoI C'TCGA_G
Cuts at: 1943 2601 1943
Size: 658 5889
XmaI C'CCGG_G
Cuts at: 1870 2883 3376 3872 1870
Size: 1013 493 496 4545
Fragments arranged by size:
4545 1013 496 493
XmnI GAAnn'nnTTC
Cuts at: 4676 4676
Size: 6547
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4355 276
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacII SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI
BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PpuMI PspXI RsrII
SacI SanDI SexAI SfoI SwaI