Restriction map for
pFN21AB7837
(Circular) (Six-base) MAPSORT of: pFN21AB7837.seq Check: 8176 from: 1 to: 6325
pFN21AB7837 6325 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3668 1937
Size: 1731 4594
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2434 2854 2938 3358 3606 4388 5772 6040
Size: 802 420 84 420 248 782 1384 268
Cuts at: 6040 1632
Size: 1917
Fragments arranged by size:
1917 1384 802 782 420 420 268 248
84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6128
AflIII A'CryG_T
Cuts at: 1203 2426 3570 5224 6201 1203
Size: 1223 1144 1654 977 1327
Fragments arranged by size:
1654 1327 1223 1144 977
AgeI A'CCGG_T
Cuts at: 5156 5156
Size: 6325
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 2997 3417 1166
Size: 1831 420 4074
Fragments arranged by size:
4074 1831 420
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6325
AlwNI CAG_nnn'CTG
Cuts at: 2278 4068 5640 2278
Size: 1790 1572 2963
Fragments arranged by size:
2963 1790 1572
ApaLI G'TGCA_C
Cuts at: 4382 5538 4382
Size: 1156 5169
ApoI r'AATT_y
Cuts at: 1493 2250 2820 2988 3634 3860 4012 6121 1493
Size: 757 570 168 646 226 152 2109 1697
Fragments arranged by size:
2109 1697 757 646 570 226 168 152
AseI AT'TA_AT
Cuts at: 160 1032 4879 160
Size: 872 3847 1606
Fragments arranged by size:
3847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 3650 1870
Size: 73 1707 4545
Fragments arranged by size:
4545 1707 73
BamHI G'GATC_C
Cuts at: 3655 4022 3655
Size: 367 5958
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3646 5101 618
Size: 325 217 627 1859 1455 1842
Fragments arranged by size:
1859 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3644 1391
Size: 2253 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2563 2731 3151 928
Size: 971 664 168 420 4102
Fragments arranged by size:
4102 971 664 420 168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4516 4550 1903
Size: 34 2579 34 3678
Fragments arranged by size:
3678 2579 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3140 4217 5427 3140
Size: 1077 1210 4038
Fragments arranged by size:
4038 1210 1077
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6325
BfrBI ATG'CAT
Cuts at: 3609 3609
Size: 6325
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4936 136
Size: 107 122 71 631 605 3264 1525
Fragments arranged by size:
3264 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3041 3461 6320 3041
Size: 420 2859 3046
Fragments arranged by size:
3046 2859 420
BlpI GC'TnA_GC
Cuts at: 3742 3742
Size: 6325
Bme1580I G_kGCm'C
Cuts at: 1790 4386 5542 1790
Size: 2596 1156 2573
Fragments arranged by size:
2596 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6325
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2622 4056 5014
Size: 416 53 83 399 24 1205 1434 958
Cuts at: 5014 442
Size: 1753
Fragments arranged by size:
1753 1434 1205 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 2519 2321
Size: 198 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2316 2514 2682 2766 2850 2934 3102 3186
Size: 1077 198 168 84 84 84 168 84
Cuts at: 3186 3270 3354 4985 1239
Size: 84 84 1631 2579
Fragments arranged by size:
2579 1631 1077 198 168 168 84 84
84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4450 5315 5613 4450
Size: 865 298 5162
Fragments arranged by size:
5162 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4988 882
Size: 617 3489 2219
Fragments arranged by size:
3489 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2429 4116 493
Size: 711 1225 1687 2702
Fragments arranged by size:
2702 1687 1225 711
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4132 4514 5149 275
Size: 53 83 186 936 2599 382 635 1451
Fragments arranged by size:
2599 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4758 5156 5430 5577 5988 4758
Size: 398 274 147 411 5095
Fragments arranged by size:
5095 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6100
Fragments arranged by size:
6100 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 5445
BseYI C'CCAG_C
Cuts at: 5528 6024 5528
Size: 496 5829
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5752
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3775 4536 5564 1540
Size: 275 186 1774 761 1028 2301
Fragments arranged by size:
2301 1774 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4386 4471 5542 4386
Size: 85 1071 5169
Fragments arranged by size:
5169 1071 85
BsmI GAATG_Cn'
Cuts at: 2936 3119 3836 3929 2936
Size: 183 717 93 5332
Fragments arranged by size:
5332 717 183 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3644 4386 4471 5542 1391
Size: 399 1854 742 85 1071 2174
Fragments arranged by size:
2174 1854 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5988 5988
Size: 6325
BspHI T'CATG_A
Cuts at: 4214 5944 4214
Size: 1730 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3680 844
Size: 2836 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4212 1394
Size: 429 2389 3507
Fragments arranged by size:
3507 2389 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2861 3365 3517 4820 4994 65
Size: 1183 1613 504 152 1303 174 1396
Fragments arranged by size:
1613 1396 1303 1183 504 174 152
BsrFI r'CCGG_y
Cuts at: 1700 1810 4969 5156 1700
Size: 110 3159 187 2869
Fragments arranged by size:
3159 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 4385 5397 4385
Size: 1012 5313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 2470 2638 2722 3226 3310 3394 5129 2470
Size: 168 84 504 84 84 1735 3666
Fragments arranged by size:
3666 1735 504 168 84 84 84
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6325
BstYI r'GATC_y
Cuts at: 1972 2068 3041 3461 3655 4022 4407 4424 5876
Size: 96 973 420 194 367 385 17 1452
Cuts at: 5876 5962 5974 6320 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 973 420 385 367 346 194
96 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4834
Fragments arranged by size:
4834 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3457 487
Size: 683 56 2231 3355
Fragments arranged by size:
3355 2231 683 56
BtsI GCAGTG_nn'
Cuts at: 2945 3841 4635 4655 2945
Size: 896 794 20 4615
Fragments arranged by size:
4615 896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2923 2958 3511 3546 529
Size: 35 2359 35 553 35 3308
Fragments arranged by size:
3308 2359 553 35 35 35
DraI TTT'AAA
Cuts at: 2845 3629 2845
Size: 784 5541
DraIII CAC_nnn'GTG
Cuts at: 2576 2576
Size: 6325
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5332 809
Size: 507 4016 1802
Fragments arranged by size:
4016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3772 4661 6290
Size: 54 1425 50 275 18 1942 889 1629
Cuts at: 6290 8
Size: 43
Fragments arranged by size:
1942 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3772 1537
Size: 275 1960 4090
Fragments arranged by size:
4090 1960 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4255 1353
Size: 2902 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4912 5286 5432 387
Size: 1064 138 3323 374 146 1280
Fragments arranged by size:
3323 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2316 2434 2514 2682 2766 2850 2854
Size: 393 684 118 80 168 84 84 4
Cuts at: 2854 2934 2938 3102 3186 3270 3354 3358 3606
Size: 80 4 164 84 84 84 4 248
Cuts at: 3606 4388 4985 5772 6040 1239
Size: 782 597 787 268 1524
Fragments arranged by size:
1524 787 782 684 597 393 268 248
168 164 118 84 84 84 84 84
80 80 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 3173 3173
Size: 6325
EcoRI G'AATT_C
Cuts at: 2820 2988 3634 4012 2820
Size: 168 646 378 5133
Fragments arranged by size:
5133 646 378 168
FspI TGC'GCA
Cuts at: 4831 4831
Size: 6325
HaeII r_GCGC'y
Cuts at: 1678 5472 1678
Size: 3794 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2550 2582 4966 4998 5040
Size: 32 135 32 978 32 2384 32 42
Cuts at: 5040 5072 1373
Size: 32 2626
Fragments arranged by size:
2626 2384 978 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3576 3669 3915 669
Size: 988 281 1638 93 246 3079
Fragments arranged by size:
3079 1638 988 281 246 93
HindIII A'AGCT_T
Cuts at: 748 2613 3285 748
Size: 1865 672 3788
Fragments arranged by size:
3788 1865 672
HpaI GTT'AAC
Cuts at: 3576 3915 3576
Size: 339 5986
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2420 5439 5623 1305
Size: 1115 3019 184 2007
Fragments arranged by size:
3019 2007 1115 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4503
Fragments arranged by size:
4503 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2593 2997 3417 4283 4642 4801 518
Size: 648 1427 404 420 866 359 159 2042
Fragments arranged by size:
2042 1427 866 648 420 404 359 159
MspA1I CmG'CkG
Cuts at: 1606 3741 4418 5566 5811 1606
Size: 2135 677 1148 245 2120
Fragments arranged by size:
2135 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4834
Fragments arranged by size:
4834 939 552
NdeI CA'TA_TG
Cuts at: 387 2202 387
Size: 1815 4510
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NsiI A_TGCA'T
Cuts at: 3611 3611
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 3574 3584 3613 3683 5228 1132
Size: 97 2345 10 29 70 1545 2229
Fragments arranged by size:
2345 2229 1545 97 70 29 10
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6325
PciI A'CATG_T
Cuts at: 3570 5224 3570
Size: 1654 4671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6325
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3173 3173
Size: 6325
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 2760 3180 3603 3895 2760
Size: 420 423 292 5190
Fragments arranged by size:
5190 423 420 292
PstI C_TGCA'G
Cuts at: 830 3677 830
Size: 2847 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
SalI G'TCGA_C
Cuts at: 1936 3667 1936
Size: 1731 4594
SbfI CC_TGCA'GG
Cuts at: 3677 3677
Size: 6325
ScaI AGT'ACT
Cuts at: 4573 6285 4573
Size: 1712 4613
SfcI C'TryA_G
Cuts at: 826 1045 2089 2130 3673 4808 5489 5680 826
Size: 219 1044 41 1543 1135 681 191 1471
Fragments arranged by size:
1543 1471 1135 1044 681 219 191 41
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6325
SmaI CCC'GGG
Cuts at: 1872 3652 1872
Size: 1780 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4429 5330 5592 820
Size: 197 926 2486 901 262 1553
Fragments arranged by size:
2486 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 3683 1132
Size: 2551 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 2951 1517
Size: 1434 4891
StyI C'CwwG_G
Cuts at: 513 1065 2004 3764 513
Size: 552 939 1760 3074
Fragments arranged by size:
3074 1760 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 4352 4369 4522 4707 5152 325
Size: 1255 480 2292 17 153 185 445 1498
Fragments arranged by size:
2292 1498 1255 480 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4571 6283 96
Size: 274 80 33 51 1443 2594 1712 138
Fragments arranged by size:
2594 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6285 6317 6285
Size: 32 6293
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6325
XbaI T'CTAG_A
Cuts at: 3661 3661
Size: 6325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2119 2011
Size: 108 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6325
XmaI C'CCGG_G
Cuts at: 1870 3650 1870
Size: 1780 4545
XmnI GAAnn'nnTTC
Cuts at: 2447 2820 2951 2988 3008 3371 3408 4454 2447
Size: 373 131 37 20 363 37 1046 4318
Fragments arranged by size:
4318 1046 373 363 131 37 37 20
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI