Restriction map for
pFN21AB7836
(Circular) (Six-base) MAPSORT of: pFN21AB7836.seq Check: 681 from: 1 to: 6541
pFN21AB7836 6541 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4351 278
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3884 1937
Size: 1947 4594
Acc65I G'GTAC_C
Cuts at: 3862 3862
Size: 6541
AclI AA'CG_TT
Cuts at: 1563 4668 5041 1563
Size: 3105 373 3063
Fragments arranged by size:
3105 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2824 2908 3160 3412 4604 5988 6256 1632
Size: 1192 84 252 252 1192 1384 268 1917
Fragments arranged by size:
1917 1384 1192 1192 268 252 252 84
AflII C'TTAA_G
Cuts at: 820 1017 3343 820
Size: 197 2326 4018
Fragments arranged by size:
4018 2326 197
AflIII A'CryG_T
Cuts at: 1203 2657 3665 5440 6417 1203
Size: 1454 1008 1775 977 1327
Fragments arranged by size:
1775 1454 1327 1008 977
AgeI A'CCGG_T
Cuts at: 5372 5372
Size: 6541
AhdI GACnn_n'nnGTC
Cuts at: 5270 5270
Size: 6541
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6541
AlwNI CAG_nnn'CTG
Cuts at: 4284 5856 4284
Size: 1572 4969
ApaLI G'TGCA_C
Cuts at: 4598 5754 4598
Size: 1156 5385
ApoI r'AATT_y
Cuts at: 1493 2422 2489 2596 2602 3042 3126 3210 3378
Size: 929 67 107 6 440 84 84 168
Cuts at: 3378 3546 3714 3798 3850 4076 4228 6337 1493
Size: 168 168 84 52 226 152 2109 1697
Fragments arranged by size:
2109 1697 929 440 226 168 168 168
152 107 84 84 84 67 52 6
AseI AT'TA_AT
Cuts at: 160 1032 5095 160
Size: 872 4063 1606
Fragments arranged by size:
4063 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
AvaI C'yCGr_G
Cuts at: 1870 1943 3866 1870
Size: 73 1923 4545
Fragments arranged by size:
4545 1923 73
BamHI G'GATC_C
Cuts at: 3871 4238 3871
Size: 367 6174
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3862 5317 618
Size: 325 217 627 2075 1455 1842
Fragments arranged by size:
2075 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3860 1391
Size: 2469 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2512 928
Size: 971 613 4957
Fragments arranged by size:
4957 971 613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4732 4766 1903
Size: 34 2795 34 3678
Fragments arranged by size:
3678 2795 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4433 5643 4433
Size: 1210 5331
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6541
BfrBI ATG'CAT
Cuts at: 3466 3749 3466
Size: 283 6258
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5152 136
Size: 107 122 71 631 605 3480 1525
Fragments arranged by size:
3480 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6536 6536
Size: 6541
BlpI GC'TnA_GC
Cuts at: 3958 3958
Size: 6541
Bme1580I G_kGCm'C
Cuts at: 1790 4602 5758 1790
Size: 2812 1156 2573
Fragments arranged by size:
2812 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2059 1318
Size: 741 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 4272 5230
Size: 416 53 83 399 24 754 2101 958
Cuts at: 5230 442
Size: 1753
Fragments arranged by size:
2101 1753 958 754 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6541
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3207 3459 3207
Size: 252 6289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2736 3156 3324 3408 3492 3576 3744
Size: 917 580 420 168 84 84 84 168
Cuts at: 3744 5201 1239
Size: 1457 2579
Fragments arranged by size:
2579 1457 917 580 420 168 168 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2952 4666 5531 5829 2952
Size: 1714 865 298 3664
Fragments arranged by size:
3664 1714 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2161 5204 882
Size: 617 662 3043 2219
Fragments arranged by size:
3043 2219 662 617
BsaAI yAC'GTr
Cuts at: 493 1204 4332 493
Size: 711 3128 2702
Fragments arranged by size:
3128 2702 711
BsaBI GATnn'nnATC
Cuts at: 6535 6535
Size: 6541
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2247 4348 4730 5365
Size: 53 83 186 936 714 2101 382 635
Cuts at: 5365 275
Size: 1451
Fragments arranged by size:
2101 1451 936 714 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3218 4974 5372 5646 5793 6204 3218
Size: 1756 398 274 147 411 3555
Fragments arranged by size:
3555 1756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6316
Fragments arranged by size:
6316 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2242 1186
Size: 1056 5485
BseYI C'CCAG_C
Cuts at: 2012 5744 6240 2012
Size: 3732 496 2313
Fragments arranged by size:
3732 2313 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3627 1163
Size: 573 1891 4077
Fragments arranged by size:
4077 1891 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3991 4752 5780 1540
Size: 275 186 1990 761 1028 2301
Fragments arranged by size:
2301 1990 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 2273 4602 4687 5758 2070
Size: 203 2329 85 1071 2853
Fragments arranged by size:
2853 2329 1071 203 85
BsmI GAATG_Cn'
Cuts at: 3468 4052 4145 3468
Size: 584 93 5864
Fragments arranged by size:
5864 584 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2254 2254
Size: 6541
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2273 3860 4602 4687 5758 1391
Size: 399 280 203 1587 742 85 1071 2174
Fragments arranged by size:
2174 1587 1071 742 399 280 203 85
BspEI T'CCGG_A
Cuts at: 6204 6204
Size: 6541
BspHI T'CATG_A
Cuts at: 3370 4430 6160 3370
Size: 1060 1730 3751
Fragments arranged by size:
3751 1730 1060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3896 844
Size: 3052 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3443 4428 1394
Size: 429 1620 985 3507
Fragments arranged by size:
3507 1620 985 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3247 5036 5210 65
Size: 1183 1999 1789 174 1396
Fragments arranged by size:
1999 1789 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5185 5372 1700
Size: 110 3375 187 2869
Fragments arranged by size:
3375 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6541
BssSI C'ACGA_G
Cuts at: 4601 5613 4601
Size: 1012 5529
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6541
BstBI TT'CG_AA
Cuts at: 5345 5345
Size: 6541
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6541
BstYI r'GATC_y
Cuts at: 1972 2215 2372 3871 4238 4623 4640 6092 6178
Size: 243 157 1499 367 385 17 1452 86
Cuts at: 6178 6190 6536 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1499 1452 385 367 346 243 157
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5050
Fragments arranged by size:
5050 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3510 487
Size: 683 56 2284 3518
Fragments arranged by size:
3518 2284 683 56
BtsI GCAGTG_nn'
Cuts at: 4057 4851 4871 4057
Size: 794 20 5727
Fragments arranged by size:
5727 794 20
ClaI AT'CG_AT
Cuts at: 1554 3571 1554
Size: 2017 4524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3229 3264 529
Size: 35 2665 35 3806
Fragments arranged by size:
3806 2665 35 35
DraI TTT'AAA
Cuts at: 2731 2748 3151 3655 3845 2731
Size: 17 403 504 190 5427
Fragments arranged by size:
5427 504 403 190 17
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5548 809
Size: 507 4232 1802
Fragments arranged by size:
4232 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2494 3598 3988
Size: 54 1425 50 275 18 664 1104 390
Cuts at: 3988 4877 6506 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1104 889 664 390 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3988 1537
Size: 275 2176 4090
Fragments arranged by size:
4090 2176 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2382 3804 4471 1353
Size: 1029 1422 667 3423
Fragments arranged by size:
3423 1422 1029 667
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5128 5502 5648 387
Size: 1064 138 3539 374 146 1280
Fragments arranged by size:
3539 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2736 2824 2908 3156 3160 3324
Size: 393 524 580 88 84 248 4 164
Cuts at: 3324 3408 3412 3492 3576 3744 4604 5201 5988
Size: 84 4 80 84 168 860 597 787
Cuts at: 5988 6256 1239
Size: 268 1524
Fragments arranged by size:
1524 860 787 597 580 524 393 268
248 168 164 88 84 84 84 80
4 4
EcoO109I rG'GnC_Cy
Cuts at: 2031 2874 2031
Size: 843 5698
EcoRI G'AATT_C
Cuts at: 2596 3126 3210 3378 3546 3714 3850 4228 2596
Size: 530 84 168 168 168 136 378 4909
Fragments arranged by size:
4909 530 378 168 168 168 136 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3082 3082
Size: 6541
FspI TGC'GCA
Cuts at: 5047 5047
Size: 6541
HaeII r_GCGC'y
Cuts at: 1678 5688 1678
Size: 4010 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5182 5214 5256 5288 1373
Size: 32 135 32 3610 32 42 32 2626
Fragments arranged by size:
3610 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2292 3885 4131 669
Size: 988 281 354 1593 246 3079
Fragments arranged by size:
3079 1593 988 354 281 246
HindIII A'AGCT_T
Cuts at: 748 2751 748
Size: 2003 4538
HpaI GTT'AAC
Cuts at: 4131 4131
Size: 6541
KpnI G_GTAC'C
Cuts at: 3866 3866
Size: 6541
MfeI C'AATT_G
Cuts at: 4140 4140
Size: 6541
MluI A'CGCG_T
Cuts at: 6417 6417
Size: 6541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5655 5839 1305
Size: 4350 184 2007
Fragments arranged by size:
4350 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2496 3600 10
Size: 54 1425 343 664 1104 2951
Fragments arranged by size:
2951 1425 1104 664 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2481 2563 3403 3514 3571 4499 4858
Size: 648 1315 82 840 111 57 928 359
Cuts at: 4858 5017 518
Size: 159 2042
Fragments arranged by size:
2042 1315 928 840 648 359 159 111
82 57
MspA1I CmG'CkG
Cuts at: 1606 2020 3957 4634 5782 6027 1606
Size: 414 1937 677 1148 245 2120
Fragments arranged by size:
2120 1937 1148 677 414 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5050
Fragments arranged by size:
5050 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6541
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6541
NotI GC'GGCC_GC
Cuts at: 3988 3988
Size: 6541
NsiI A_TGCA'T
Cuts at: 3468 3751 3468
Size: 283 6258
NspI r_CATG'y
Cuts at: 1132 1229 2661 3669 3753 3839 3899 5444 1132
Size: 97 1432 1008 84 86 60 1545 2229
Fragments arranged by size:
2229 1545 1432 1008 97 86 84 60
PasI CC'CwG_GG
Cuts at: 1348 2220 1348
Size: 872 5669
PciI A'CATG_T
Cuts at: 2657 3665 5440 2657
Size: 1008 1775 3758
Fragments arranged by size:
3758 1775 1008
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2884 1108
Size: 1776 4765
PmeI GTTT'AAAC
Cuts at: 3845 3845
Size: 6541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2173 4610 4642 6123 6155 2141
Size: 32 2437 32 1481 32 2527
Fragments arranged by size:
2527 2437 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2874 2874
Size: 6541
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6541
PsiI TTA'TAA
Cuts at: 3795 4111 3795
Size: 316 6225
PstI C_TGCA'G
Cuts at: 830 3893 830
Size: 3063 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6541
SalI G'TCGA_C
Cuts at: 1936 3883 1936
Size: 1947 4594
SbfI CC_TGCA'GG
Cuts at: 3893 3893
Size: 6541
ScaI AGT'ACT
Cuts at: 4789 6501 4789
Size: 1712 4829
SexAI A'CCwGG_T
Cuts at: 2151 2151
Size: 6541
SfcI C'TryA_G
Cuts at: 826 1045 2143 2463 2896 3232 3889 5024 5705
Size: 219 1098 320 433 336 657 1135 681
Cuts at: 5705 5896 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1098 681 657 433 336 320
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6541
SmaI CCC'GGG
Cuts at: 1872 3868 1872
Size: 1996 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2967 3343 4645 5546 5808 820
Size: 197 926 1024 376 1302 901 262 1553
Fragments arranged by size:
1553 1302 1024 926 901 376 262 197
SnaBI TAC'GTA
Cuts at: 493 4332 493
Size: 3839 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6541
SphI G_CATG'C
Cuts at: 1132 3899 1132
Size: 2767 3774
SspI AAT'ATT
Cuts at: 5 52 4465 5
Size: 47 4413 2081
Fragments arranged by size:
4413 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6541
StyI C'CwwG_G
Cuts at: 513 1065 2004 3980 513
Size: 552 939 1976 3074
Fragments arranged by size:
3074 1976 939 552
SwaI ATTT'AAAT
Cuts at: 2731 2731
Size: 6541
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4568 4585 4738 4923 5368 325
Size: 1255 2988 17 153 185 445 1498
Fragments arranged by size:
2988 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2654 4787 6499
Size: 274 80 33 51 1443 677 2133 1712
Cuts at: 6499 96
Size: 138
Fragments arranged by size:
2133 1712 1443 677 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2669 2701 6501 6533 2669
Size: 32 3800 32 2677
Fragments arranged by size:
3800 2677 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2287 1316
Size: 971 5570
XbaI T'CTAG_A
Cuts at: 3877 3877
Size: 6541
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6541
XmaI C'CCGG_G
Cuts at: 1870 3866 1870
Size: 1996 4545
XmnI GAAnn'nnTTC
Cuts at: 2837 3210 4670 2837
Size: 373 1460 4708
Fragments arranged by size:
4708 1460 373
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4349 276
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
XcmI