Restriction map for
pFN21AB7835
(Circular) (Six-base) MAPSORT of: pFN21AB7835.seq Check: 5608 from: 1 to: 6325
pFN21AB7835 6325 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3591 3591
Size: 6325
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2103 3229 3668 1937
Size: 166 1126 439 4594
Fragments arranged by size:
4594 1126 439 166
Acc65I G'GTAC_C
Cuts at: 2350 3646 2350
Size: 1296 5029
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4388 5772 6040 1632
Size: 2756 1384 268 1917
Fragments arranged by size:
2756 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6128
AflIII A'CryG_T
Cuts at: 1203 2127 5224 6201 1203
Size: 924 3097 977 1327
Fragments arranged by size:
3097 1327 977 924
AgeI A'CCGG_T
Cuts at: 5156 5156
Size: 6325
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2259 1184
Size: 1075 5250
AlwNI CAG_nnn'CTG
Cuts at: 4068 5640 4068
Size: 1572 4753
ApaLI G'TGCA_C
Cuts at: 2244 4382 5538 2244
Size: 2138 1156 3031
Fragments arranged by size:
3031 2138 1156
ApoI r'AATT_y
Cuts at: 1493 2454 2951 3197 3634 3860 4012 6121 1493
Size: 961 497 246 437 226 152 2109 1697
Fragments arranged by size:
2109 1697 961 497 437 246 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2747 4879 160
Size: 872 1715 2132 1606
Fragments arranged by size:
2132 1715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 3650 1870
Size: 73 1707 4545
Fragments arranged by size:
4545 1707 73
AvrII C'CTAG_G
Cuts at: 2411 2411
Size: 6325
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2334 2367 2334
Size: 33 6292
BamHI G'GATC_C
Cuts at: 3006 3655 4022 3006
Size: 649 367 5309
Fragments arranged by size:
5309 649 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2350 3448 3646 5101 618
Size: 325 217 627 563 1098 198 1455 1842
Fragments arranged by size:
1842 1455 1098 627 563 325 217 198
BanII G_rGCy'C
Cuts at: 1391 2773 3644 1391
Size: 1382 871 4072
Fragments arranged by size:
4072 1382 871
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 3333 928
Size: 971 237 1197 3920
Fragments arranged by size:
3920 1197 971 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2531 2565 4516 4550 1903
Size: 34 594 34 1951 34 3678
Fragments arranged by size:
3678 1951 594 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4217 5427 4217
Size: 1210 5115
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6325
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4936 136
Size: 107 122 71 631 605 3264 1525
Fragments arranged by size:
3264 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6320 6320
Size: 6325
BlpI GC'TnA_GC
Cuts at: 3612 3742 3612
Size: 130 6195
Bme1580I G_kGCm'C
Cuts at: 1790 2248 3451 4386 5542 1790
Size: 458 1203 935 1156 2573
Fragments arranged by size:
2573 1203 1156 935 458
BmgBI CAC'GTC
Cuts at: 1318 2332 1318
Size: 1014 5311
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4056 5014 442
Size: 416 53 83 399 24 2639 958 1753
Fragments arranged by size:
2639 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2845 2979 3544 4985 1239
Size: 1606 134 565 1441 2579
Fragments arranged by size:
2579 1606 1441 565 134
Bpu10I CC'TnA_GC
Cuts at: 3537 3537
Size: 6325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3030 3219 4450 5315 5613 3030
Size: 189 1231 865 298 3742
Fragments arranged by size:
3742 1231 865 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4988 882
Size: 617 3489 2219
Fragments arranged by size:
3489 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4116 493
Size: 711 2912 2702
Fragments arranged by size:
2912 2702 711
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4132 4514 5149 275
Size: 53 83 186 936 2599 382 635 1451
Fragments arranged by size:
2599 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4758 5156 5430 5577 5988 4758
Size: 398 274 147 411 5095
Fragments arranged by size:
5095 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3351 3381 1178
Size: 30 165 30 1948 30 4122
Fragments arranged by size:
4122 1948 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3375 1186
Size: 2189 4136
BseYI C'CCAG_C
Cuts at: 2695 5528 6024 2695
Size: 2833 496 2996
Fragments arranged by size:
2996 2833 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5752
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3775 4536 5564 1540
Size: 275 186 1774 761 1028 2301
Fragments arranged by size:
2301 1774 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2248 2773 4386 4471 5542 2248
Size: 525 1613 85 1071 3031
Fragments arranged by size:
3031 1613 1071 525 85
BsmI GAATG_Cn'
Cuts at: 3836 3929 3836
Size: 93 6232
BsmBI CGTCTCn'nnnn_
Cuts at: 3357 3357
Size: 6325
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2248 2773 3451 3644 4386 4471 5542
Size: 399 458 525 678 193 742 85 1071
Cuts at: 5542 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 678 525 458 399 193
85
BspEI T'CCGG_A
Cuts at: 5988 5988
Size: 6325
BspHI T'CATG_A
Cuts at: 4214 5944 4214
Size: 1730 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3591 3680 844
Size: 2747 89 3489
Fragments arranged by size:
3489 2747 89
BsrBI CCG'CTC
Cuts at: 1394 1823 4212 1394
Size: 429 2389 3507
Fragments arranged by size:
3507 2389 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4820 4994 65
Size: 1183 3572 174 1396
Fragments arranged by size:
3572 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4969 5156 1700
Size: 110 3159 187 2869
Fragments arranged by size:
3159 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 4385 5397 4385
Size: 1012 5313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 2732 1184
Size: 1548 4777
BstYI r'GATC_y
Cuts at: 1972 2872 3006 3655 4022 4407 4424 5876 5962
Size: 900 134 649 367 385 17 1452 86
Cuts at: 5962 5974 6320 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 900 649 385 367 346 134
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4834
Fragments arranged by size:
4834 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5586
Fragments arranged by size:
5586 683 56
BtsI GCAGTG_nn'
Cuts at: 3393 3841 4635 4655 3393
Size: 448 794 20 5063
Fragments arranged by size:
5063 794 448 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6290
DraI TTT'AAA
Cuts at: 3629 3629
Size: 6325
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5332 809
Size: 507 4016 1802
Fragments arranged by size:
4016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3313 3772 4661
Size: 54 1425 50 275 18 1483 459 889
Cuts at: 4661 6290 8
Size: 1629 43
Fragments arranged by size:
1629 1483 1425 889 459 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3772 1537
Size: 275 1960 4090
Fragments arranged by size:
4090 1960 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2761 3341 4255 1353
Size: 1408 580 914 3423
Fragments arranged by size:
3423 1408 914 580
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4912 5286 5432 387
Size: 1064 138 3323 374 146 1280
Fragments arranged by size:
3323 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2771 2771
Size: 6325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2845 2979 3544 4388 4985 5772 6040
Size: 393 1213 134 565 844 597 787 268
Cuts at: 6040 1239
Size: 1524
Fragments arranged by size:
1524 1213 844 787 597 565 393 268
134
EcoNI CCTnn'n_nnAGG
Cuts at: 3487 3487
Size: 6325
EcoRI G'AATT_C
Cuts at: 2454 3634 4012 2454
Size: 1180 378 4767
Fragments arranged by size:
4767 1180 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2790 2790
Size: 6325
FspI TGC'GCA
Cuts at: 4831 4831
Size: 6325
HaeII r_GCGC'y
Cuts at: 1678 5472 1678
Size: 3794 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2864 2885 2896 2917 4966
Size: 32 135 32 1292 21 11 21 2049
Cuts at: 4966 4998 5040 5072 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2049 1292 135 42 32 32 32
32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2104 3087 3230 3669 3915 669
Size: 988 281 166 983 143 439 246 3079
Fragments arranged by size:
3079 988 983 439 281 246 166 143
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6325
HpaI GTT'AAC
Cuts at: 3087 3915 3087
Size: 828 5497
KpnI G_GTAC'C
Cuts at: 2354 3650 2354
Size: 1296 5029
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2761 2900 5439 5623 1305
Size: 1456 139 2539 184 2007
Fragments arranged by size:
2539 2007 1456 184 139
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4503
Fragments arranged by size:
4503 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4283 4642 4801 518
Size: 648 3117 359 159 2042
Fragments arranged by size:
3117 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 3741 4418 5566 5811 1606
Size: 600 1535 677 1148 245 2120
Fragments arranged by size:
2120 1535 1148 677 600 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4834
Fragments arranged by size:
4834 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6325
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 2068 2131 3683 5228 1132
Size: 97 839 63 1552 1545 2229
Fragments arranged by size:
2229 1552 1545 839 97 63
PasI CC'CwG_GG
Cuts at: 1348 3210 1348
Size: 1862 4463
PciI A'CATG_T
Cuts at: 2127 5224 2127
Size: 3097 3228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6325
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 3895 3895
Size: 6325
PstI C_TGCA'G
Cuts at: 830 3388 3677 830
Size: 2558 289 3478
Fragments arranged by size:
3478 2558 289
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
PvuII CAG'CTG
Cuts at: 2206 2206
Size: 6325
SacI G_AGCT'C
Cuts at: 2773 2773
Size: 6325
SalI G'TCGA_C
Cuts at: 1936 2102 3228 3667 1936
Size: 166 1126 439 4594
Fragments arranged by size:
4594 1126 439 166
SapI GCTCTTCn'nnn_
Cuts at: 2761 2761
Size: 6325
SbfI CC_TGCA'GG
Cuts at: 3677 3677
Size: 6325
ScaI AGT'ACT
Cuts at: 3266 4573 6285 3266
Size: 1307 1712 3306
Fragments arranged by size:
3306 1712 1307
SexAI A'CCwGG_T
Cuts at: 2049 2049
Size: 6325
SfcI C'TryA_G
Cuts at: 826 1045 2134 3384 3673 4808 5489 5680 826
Size: 219 1089 1250 289 1135 681 191 1471
Fragments arranged by size:
1471 1250 1135 1089 681 289 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6325
SmaI CCC'GGG
Cuts at: 1872 3652 1872
Size: 1780 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3045 3234 4429 5330 5592 820
Size: 197 926 1102 189 1195 901 262 1553
Fragments arranged by size:
1553 1195 1102 926 901 262 197 189
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 3683 1132
Size: 2551 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 2253 3162 1517
Size: 736 909 4680
Fragments arranged by size:
4680 909 736
StyI C'CwwG_G
Cuts at: 513 1065 2004 2411 2757 3764 513
Size: 552 939 407 346 1007 3074
Fragments arranged by size:
3074 1007 939 552 407 346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2151 2724 4352 4369 4522 4707 5152
Size: 1255 571 573 1628 17 153 185 445
Cuts at: 5152 325
Size: 1498
Fragments arranged by size:
1628 1498 1255 573 571 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 2936 3264
Size: 274 80 33 51 1443 153 806 328
Cuts at: 3264 4571 6283 96
Size: 1307 1712 138
Fragments arranged by size:
1712 1443 1307 806 328 274 153 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 6285 6317 3572
Size: 32 2681 32 3580
Fragments arranged by size:
3580 2681 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6325
XbaI T'CTAG_A
Cuts at: 3661 3661
Size: 6325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2435 2435
Size: 6325
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6325
XmaI C'CCGG_G
Cuts at: 1870 3650 1870
Size: 1780 4545
XmnI GAAnn'nnTTC
Cuts at: 2841 3330 4454 2841
Size: 489 1124 4712
Fragments arranged by size:
4712 1124 489
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI