Restriction map for
pFN21AB7834
(Circular) (Six-base) MAPSORT of: pFN21AB7834.seq Check: 1483 from: 1 to: 6538
pFN21AB7834 6538 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3097 4348 278
Size: 53 83 186 936 1561 1251 2468
Fragments arranged by size:
2468 1561 1251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3881 1937
Size: 1944 4594
Acc65I G'GTAC_C
Cuts at: 3859 3859
Size: 6538
AclI AA'CG_TT
Cuts at: 1563 4665 5038 1563
Size: 3102 373 3063
Fragments arranged by size:
3102 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2501 2940 3093 3674 4601 5985 6253 1632
Size: 869 439 153 581 927 1384 268 1917
Fragments arranged by size:
1917 1384 927 869 581 439 268 153
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6341
AflIII A'CryG_T
Cuts at: 1203 2472 5437 6414 1203
Size: 1269 2965 977 1327
Fragments arranged by size:
2965 1327 1269 977
AgeI A'CCGG_T
Cuts at: 5369 5369
Size: 6538
AhdI GACnn_n'nnGTC
Cuts at: 2241 5267 2241
Size: 3026 3512
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6538
AlwNI CAG_nnn'CTG
Cuts at: 2315 3570 3742 4281 5853 2315
Size: 1255 172 539 1572 3000
Fragments arranged by size:
3000 1572 1255 539 172
ApaLI G'TGCA_C
Cuts at: 4595 5751 4595
Size: 1156 5382
ApoI r'AATT_y
Cuts at: 1493 2818 3110 3397 3847 4073 4225 6334 1493
Size: 1325 292 287 450 226 152 2109 1697
Fragments arranged by size:
2109 1697 1325 450 292 287 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5092 160
Size: 872 4060 1606
Fragments arranged by size:
4060 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2124 2949 3863 1870
Size: 73 132 49 825 914 4545
Fragments arranged by size:
4545 914 825 132 73 49
BamHI G'GATC_C
Cuts at: 3799 3868 4235 3799
Size: 69 367 6102
Fragments arranged by size:
6102 367 69
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2751 2758 3859 5314 618
Size: 325 217 627 964 7 1101 1455 1842
Fragments arranged by size:
1842 1455 1101 964 627 325 217 7
BanII G_rGCy'C
Cuts at: 1391 3324 3857 1391
Size: 1933 533 4072
Fragments arranged by size:
4072 1933 533
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5567
BbvCI CC'TCA_GC
Cuts at: 2140 2140
Size: 6538
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4729 4763 1903
Size: 34 50 34 2708 34 3678
Fragments arranged by size:
3678 2708 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3437 4430 5640 3437
Size: 993 1210 4335
Fragments arranged by size:
4335 1210 993
BclI T'GATC_A
Cuts at: 1548 2362 1548
Size: 814 5724
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3767 5149 136
Size: 107 122 71 631 605 2095 1382 1525
Fragments arranged by size:
2095 1525 1382 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2071 3249 3541 6533 2071
Size: 1178 292 2992 2076
Fragments arranged by size:
2992 2076 1178 292
BlpI GC'TnA_GC
Cuts at: 2150 3955 2150
Size: 1805 4733
Bme1580I G_kGCm'C
Cuts at: 1790 3836 4599 5755 1790
Size: 2046 763 1156 2573
Fragments arranged by size:
2573 2046 1156 763
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6538
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2255 4269 5227
Size: 416 53 83 399 24 838 2014 958
Cuts at: 5227 442
Size: 1753
Fragments arranged by size:
2014 1753 958 838 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2416 5198 1239
Size: 1177 2782 2579
Fragments arranged by size:
2782 2579 1177
Bpu10I CC'TnA_GC
Cuts at: 2140 2140
Size: 6538
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2148 4663 5528 5826 2148
Size: 2515 865 298 2860
Fragments arranged by size:
2860 2515 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5201 882
Size: 617 3702 2219
Fragments arranged by size:
3702 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4329 493
Size: 711 3125 2702
Fragments arranged by size:
3125 2702 711
BsaBI GATnn'nnATC
Cuts at: 3623 6532 3623
Size: 2909 3629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3094 4345 4727 5362
Size: 53 83 186 936 1561 1251 382 635
Cuts at: 5362 275
Size: 1451
Fragments arranged by size:
1561 1451 1251 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4971 5369 5643 5790 6201 4971
Size: 398 274 147 411 5308
Fragments arranged by size:
5308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3404 3434 1178
Size: 30 165 30 2001 30 4282
Fragments arranged by size:
4282 2001 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2128 2222 1186
Size: 942 94 5502
Fragments arranged by size:
5502 942 94
BseYI C'CCAG_C
Cuts at: 3088 3803 5741 6237 3088
Size: 715 1938 496 3389
Fragments arranged by size:
3389 1938 715 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5965
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3988 4749 5777 1540
Size: 275 186 1987 761 1028 2301
Fragments arranged by size:
2301 1987 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 2677 2944 4599 4684 5755 2082
Size: 595 267 1655 85 1071 2865
Fragments arranged by size:
2865 1655 1071 595 267 85
BsmI GAATG_Cn'
Cuts at: 2029 2666 4049 4142 2029
Size: 637 1383 93 4425
Fragments arranged by size:
4425 1383 637 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3101 3424 3638 3101
Size: 323 214 6001
Fragments arranged by size:
6001 323 214
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2677 2944 3324 3836 3857 4599
Size: 399 292 595 267 380 512 21 742
Cuts at: 4599 4684 5755 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 595 512 399 380 292
267 85 21
BspEI T'CCGG_A
Cuts at: 6201 6201
Size: 6538
BspHI T'CATG_A
Cuts at: 2365 4427 6157 2365
Size: 2062 1730 2746
Fragments arranged by size:
2746 2062 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3603 3893 844
Size: 2759 290 3489
Fragments arranged by size:
3489 2759 290
BsrBI CCG'CTC
Cuts at: 1394 1823 2136 4425 1394
Size: 429 313 2289 3507
Fragments arranged by size:
3507 2289 429 313
BsrDI GCAATG_nn'
Cuts at: 65 1248 2460 5033 5207 65
Size: 1183 1212 2573 174 1396
Fragments arranged by size:
2573 1396 1212 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3150 5182 5369 1700
Size: 110 1340 2032 187 2869
Fragments arranged by size:
2869 2032 1340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6538
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6538
BssSI C'ACGA_G
Cuts at: 4598 5610 4598
Size: 1012 5526
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6538
BstBI TT'CG_AA
Cuts at: 2182 5342 2182
Size: 3160 3378
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6538
BstYI r'GATC_y
Cuts at: 1972 2071 2926 3249 3541 3799 3868 4235 4620
Size: 99 855 323 292 258 69 367 385
Cuts at: 4620 4637 6089 6175 6187 6533 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 855 385 367 346 323 292
258 99 86 69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3795 513
Size: 552 2730 3256
Fragments arranged by size:
3256 2730 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2492 487
Size: 683 56 1266 4533
Fragments arranged by size:
4533 1266 683 56
BtsI GCAGTG_nn'
Cuts at: 2500 4054 4848 4868 2500
Size: 1554 794 20 4170
Fragments arranged by size:
4170 1554 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6538
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6503
DraI TTT'AAA
Cuts at: 3120 3729 3842 3120
Size: 609 113 5816
Fragments arranged by size:
5816 609 113
DraIII CAC_nnn'GTG
Cuts at: 3188 3188
Size: 6538
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5545 809
Size: 507 4229 1802
Fragments arranged by size:
4229 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3031 3159 3276
Size: 54 1425 50 275 18 1201 128 117
Cuts at: 3276 3985 4874 6503 8
Size: 709 889 1629 43
Fragments arranged by size:
1629 1425 1201 889 709 275 128 117
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3985 1537
Size: 275 2173 4090
Fragments arranged by size:
4090 2173 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 2224 2289 2932 3125 4468 1353
Size: 659 212 65 643 193 1343 3423
Fragments arranged by size:
3423 1343 659 643 212 193 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3137 3365 5125 5499 5645 387
Size: 1064 138 1548 228 1760 374 146 1280
Fragments arranged by size:
1760 1548 1280 1064 374 228 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2416 2501 2940 3093 3674 4601 5198
Size: 393 784 85 439 153 581 927 597
Cuts at: 5198 5985 6253 1239
Size: 787 268 1524
Fragments arranged by size:
1524 927 787 784 597 581 439 393
268 153 85
EcoNI CCTnn'n_nnAGG
Cuts at: 2143 2565 2143
Size: 422 6116
EcoO109I rG'GnC_Cy
Cuts at: 3739 3739
Size: 6538
EcoRI G'AATT_C
Cuts at: 3110 3397 3847 4225 3110
Size: 287 450 378 5423
Fragments arranged by size:
5423 450 378 287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3599 3599
Size: 6538
FspI TGC'GCA
Cuts at: 5044 5044
Size: 6538
HaeII r_GCGC'y
Cuts at: 1678 2521 5685 1678
Size: 843 3164 2531
Fragments arranged by size:
3164 2531 843
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2227 2259 2698
Size: 32 135 32 521 32 102 32 439
Cuts at: 2698 2730 5179 5211 5253 5285 1373
Size: 32 2449 32 42 32 2626
Fragments arranged by size:
2626 2449 521 439 135 102 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3882 4128 669
Size: 988 281 1944 246 3079
Fragments arranged by size:
3079 1944 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6538
HpaI GTT'AAC
Cuts at: 4128 4128
Size: 6538
KpnI G_GTAC'C
Cuts at: 3863 3863
Size: 6538
MfeI C'AATT_G
Cuts at: 3021 4137 3021
Size: 1116 5422
MluI A'CGCG_T
Cuts at: 6414 6414
Size: 6538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2414 3804 5652 5836 1305
Size: 1109 1390 1848 184 2007
Fragments arranged by size:
2007 1848 1390 1109 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3161 3278 10
Size: 54 1425 343 1329 117 3270
Fragments arranged by size:
3270 1425 1329 343 117 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4496 4855 5014 518
Size: 648 3330 359 159 2042
Fragments arranged by size:
3330 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2360 2654 3807 3954 4631 5779 6024 1606
Size: 754 294 1153 147 677 1148 245 2120
Fragments arranged by size:
2120 1153 1148 754 677 294 245 147
NaeI GCC'GGC
Cuts at: 3152 3152
Size: 6538
NcoI C'CATG_G
Cuts at: 513 1065 3795 513
Size: 552 2730 3256
Fragments arranged by size:
3256 2730 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6538
NgoMIV G'CCGG_C
Cuts at: 3150 3150
Size: 6538
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6538
NotI GC'GGCC_GC
Cuts at: 3985 3985
Size: 6538
NspI r_CATG'y
Cuts at: 1132 1229 2476 2744 3896 5441 1132
Size: 97 1247 268 1152 1545 2229
Fragments arranged by size:
2229 1545 1247 1152 268 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6538
PciI A'CATG_T
Cuts at: 2472 5437 2472
Size: 2965 3573
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6538
PmeI GTTT'AAAC
Cuts at: 3842 3842
Size: 6538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3519 3551 4607 4639 6120 6152 3519
Size: 32 1056 32 1481 32 3905
Fragments arranged by size:
3905 1481 1056 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3739 3739
Size: 6538
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6538
PsiI TTA'TAA
Cuts at: 3554 4108 3554
Size: 554 5984
PspXI vC'TCGA_Gb
Cuts at: 2124 2124
Size: 6538
PstI C_TGCA'G
Cuts at: 830 2193 3890 830
Size: 1363 1697 3478
Fragments arranged by size:
3478 1697 1363
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6538
PvuII CAG'CTG
Cuts at: 2360 2654 3807 2360
Size: 294 1153 5091
Fragments arranged by size:
5091 1153 294
SalI G'TCGA_C
Cuts at: 1936 3880 1936
Size: 1944 4594
SapI GCTCTTCn'nnn_
Cuts at: 2932 3125 2932
Size: 193 6345
SbfI CC_TGCA'GG
Cuts at: 3890 3890
Size: 6538
ScaI AGT'ACT
Cuts at: 3582 4786 6498 3582
Size: 1204 1712 3622
Fragments arranged by size:
3622 1712 1204
SexAI A'CCwGG_T
Cuts at: 3050 3184 3050
Size: 134 6404
SfcI C'TryA_G
Cuts at: 826 1045 2067 2189 3886 5021 5702 5893 826
Size: 219 1022 122 1697 1135 681 191 1471
Fragments arranged by size:
1697 1471 1135 1022 681 219 191 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6538
SmaI CCC'GGG
Cuts at: 1872 2951 3865 1872
Size: 1079 914 4545
Fragments arranged by size:
4545 1079 914
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2075 2124 2163 4642 5543 5805
Size: 197 926 132 49 39 2479 901 262
Cuts at: 5805 820
Size: 1553
Fragments arranged by size:
2479 1553 926 901 262 197 132 49
39
SnaBI TAC'GTA
Cuts at: 493 4329 493
Size: 3836 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6538
SphI G_CATG'C
Cuts at: 1132 2744 3896 1132
Size: 1612 1152 3774
Fragments arranged by size:
3774 1612 1152
SrfI GCCC'GGGC
Cuts at: 2951 2951
Size: 6538
SspI AAT'ATT
Cuts at: 5 52 4462 5
Size: 47 4410 2081
Fragments arranged by size:
4410 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6538
StyI C'CwwG_G
Cuts at: 513 1065 2466 3795 3828 3977 513
Size: 552 1401 1329 33 149 3074
Fragments arranged by size:
3074 1401 1329 552 149 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3195 4565 4582 4735 4920 5365 325
Size: 1255 1615 1370 17 153 185 445 1498
Fragments arranged by size:
1615 1498 1370 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3444 3580 4784
Size: 274 80 33 51 1443 1467 136 1204
Cuts at: 4784 6496 96
Size: 1712 138
Fragments arranged by size:
1712 1467 1443 1204 274 138 136 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6498 6530 6498
Size: 32 6506
Tth111I GACn'n_nGTC
Cuts at: 1316 3668 1316
Size: 2352 4186
XbaI T'CTAG_A
Cuts at: 3874 3874
Size: 6538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3286 3793 3286
Size: 507 6031
XhoI C'TCGA_G
Cuts at: 1943 2075 2124 1943
Size: 132 49 6357
Fragments arranged by size:
6357 132 49
XmaI C'CCGG_G
Cuts at: 1870 2949 3863 1870
Size: 1079 914 4545
Fragments arranged by size:
4545 1079 914
XmnI GAAnn'nnTTC
Cuts at: 3401 3582 4667 3401
Size: 181 1085 5272
Fragments arranged by size:
5272 1085 181
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3095 4346 276
Size: 53 83 186 936 1561 1251 2468
Fragments arranged by size:
2468 1561 1251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PsrI RsrII SacI SacII SanDI SfiI
SfoI SwaI