Restriction map for
pFN21AB7833
(Circular) (Six-base) MAPSORT of: pFN21AB7833.seq Check: 9521 from: 1 to: 6319
pFN21AB7833 6319 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2371 2455 3409 4382 5766 6034 1632
Size: 739 84 954 973 1384 268 1917
Fragments arranged by size:
1917 1384 973 954 739 268 84
AflII C'TTAA_G
Cuts at: 820 1017 2321 820
Size: 197 1304 4818
Fragments arranged by size:
4818 1304 197
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 3132 3468 1166
Size: 1966 336 4017
Fragments arranged by size:
4017 1966 336
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6319
AlwNI CAG_nnn'CTG
Cuts at: 2571 4062 5634 2571
Size: 1491 1572 3256
Fragments arranged by size:
3256 1572 1491
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 2209 2619 2720 2822 2918 3628 3854 4006
Size: 716 410 101 102 96 710 226 152
Cuts at: 4006 6115 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 716 710 410 226 152 102
101 96
AseI AT'TA_AT
Cuts at: 160 1032 2181 3177 4873 160
Size: 872 1149 996 1696 1606
Fragments arranged by size:
1696 1606 1149 996 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 3644 1870
Size: 73 1701 4545
Fragments arranged by size:
4545 1701 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3640 5095 618
Size: 325 217 627 1853 1455 1842
Fragments arranged by size:
1853 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3638 1391
Size: 2247 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5348
BbvCI CC'TCA_GC
Cuts at: 2202 2202
Size: 6319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4930 136
Size: 107 122 71 631 605 337 2921 1525
Fragments arranged by size:
2921 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2851 6314 2851
Size: 3463 2856
BlpI GC'TnA_GC
Cuts at: 3736 3736
Size: 6319
Bme1580I G_kGCm'C
Cuts at: 1790 4380 5536 1790
Size: 2590 1156 2573
Fragments arranged by size:
2590 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4050 5008 442
Size: 416 53 83 399 24 2633 958 1753
Fragments arranged by size:
2633 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2985 3069 4979 1239
Size: 917 829 84 1910 2579
Fragments arranged by size:
2579 1910 917 829 84
Bpu10I CC'TnA_GC
Cuts at: 2202 2202
Size: 6319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2323 2529 2691 4444 5309 5607 2323
Size: 206 162 1753 865 298 3035
Fragments arranged by size:
3035 1753 865 298 206 162
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2629 4982 882
Size: 617 1130 2353 2219
Fragments arranged by size:
2353 2219 1130 617
BsaAI yAC'GTr
Cuts at: 493 1204 4110 493
Size: 711 2906 2702
Fragments arranged by size:
2906 2702 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4126 4508 5143 275
Size: 53 83 186 936 2593 382 635 1451
Fragments arranged by size:
2593 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4752 5150 5424 5571 5982 4752
Size: 398 274 147 411 5089
Fragments arranged by size:
5089 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6094
Fragments arranged by size:
6094 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2584 3340 1186
Size: 910 488 756 4165
Fragments arranged by size:
4165 910 756 488
BseYI C'CCAG_C
Cuts at: 2012 5522 6018 2012
Size: 3510 496 2313
Fragments arranged by size:
3510 2313 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3321 1163
Size: 573 1585 4161
Fragments arranged by size:
4161 1585 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4380 4465 5536 4380
Size: 85 1071 5163
Fragments arranged by size:
5163 1071 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3638 4380 4465 5536 1391
Size: 399 1848 742 85 1071 2174
Fragments arranged by size:
2174 1848 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3674 844
Size: 2830 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4206 1394
Size: 429 2383 3507
Fragments arranged by size:
3507 2383 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4814 4988 65
Size: 1183 3566 174 1396
Fragments arranged by size:
3566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4963 5150 1700
Size: 110 3153 187 2869
Fragments arranged by size:
3153 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2692 3112 96
Size: 2596 420 3303
Fragments arranged by size:
3303 2596 420
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6319
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 2239
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 2851 3649 4016 4401 4418 5870 5956 5968
Size: 879 798 367 385 17 1452 86 12
Cuts at: 5968 6314 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 879 798 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4828
Fragments arranged by size:
4828 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2572 3835 4629 4649 2572
Size: 1263 794 20 4242
Fragments arranged by size:
4242 1263 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2974 3009 529
Size: 35 2410 35 3839
Fragments arranged by size:
3839 2410 35 35
DraI TTT'AAA
Cuts at: 3193 3623 3193
Size: 430 5889
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3766 4655 6284
Size: 54 1425 50 275 18 1936 889 1629
Cuts at: 6284 8
Size: 43
Fragments arranged by size:
1936 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3103 3190 4249 1353
Size: 1750 87 1059 3423
Fragments arranged by size:
3423 1750 1059 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2371 2455 2985 3069 3409 4382
Size: 393 524 215 84 530 84 340 973
Cuts at: 4382 4979 5766 6034 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 973 787 597 530 524 393 340
268 215 84 84
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
EcoRV GAT'ATC
Cuts at: 2428 2428
Size: 6319
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 5466 1678
Size: 3788 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4960 4992 5034 5066 1373
Size: 32 135 32 3388 32 42 32 2626
Fragments arranged by size:
3388 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2022 3239 3365 3663 3909 669
Size: 988 281 84 1217 126 298 246 3079
Fragments arranged by size:
3079 1217 988 298 281 246 126 84
HindIII A'AGCT_T
Cuts at: 748 3084 748
Size: 2336 3983
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5433 5617 1305
Size: 4128 184 2007
Fragments arranged by size:
4128 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4497
Fragments arranged by size:
4497 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2560 3132 3216 3400 3468 4277 4636
Size: 648 1394 572 84 184 68 809 359
Cuts at: 4636 4795 518
Size: 159 2042
Fragments arranged by size:
2042 1394 809 648 572 359 184 159
84 68
MspA1I CmG'CkG
Cuts at: 1606 3735 4412 5560 5805 1606
Size: 2129 677 1148 245 2120
Fragments arranged by size:
2129 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4828
Fragments arranged by size:
4828 939 552
NdeI CA'TA_TG
Cuts at: 387 3117 387
Size: 2730 3589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2701 2742 3205 3677 5222 1132
Size: 97 1472 41 463 472 1545 2229
Fragments arranged by size:
2229 1545 1472 472 463 97 41
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6319
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3026 3889 3026
Size: 863 5456
PstI C_TGCA'G
Cuts at: 830 3671 830
Size: 2841 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SfcI C'TryA_G
Cuts at: 826 1045 2679 2808 3667 4802 5483 5674 826
Size: 219 1634 129 859 1135 681 191 1471
Fragments arranged by size:
1634 1471 1135 859 681 219 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3646 1872
Size: 1774 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2302 2321 2544 2706 4423 5324
Size: 197 926 359 19 223 162 1717 901
Cuts at: 5324 5586 820
Size: 262 1553
Fragments arranged by size:
1717 1553 926 901 359 262 223 197
162 19
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 3310 3441 1517
Size: 1793 131 4395
Fragments arranged by size:
4395 1793 131
StyI C'CwwG_G
Cuts at: 513 1065 2004 3758 513
Size: 552 939 1754 3074
Fragments arranged by size:
3074 1754 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2004 2090 3504 4346 4363 4516 4701
Size: 1255 424 86 1414 842 17 153 185
Cuts at: 4701 5146 325
Size: 445 1498
Fragments arranged by size:
1498 1414 1255 842 445 424 185 153
86 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2692 3112 3448
Size: 274 80 33 51 1443 715 420 336
Cuts at: 3448 4565 6277 96
Size: 1117 1712 138
Fragments arranged by size:
1712 1443 1117 715 420 336 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 3644 1870
Size: 1774 4545
XmnI GAAnn'nnTTC
Cuts at: 2750 3002 3254 4448 2750
Size: 252 252 1194 4621
Fragments arranged by size:
4621 1194 252 252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI