Restriction map for
pFN21AB7832
(Circular) (Six-base) MAPSORT of: pFN21AB7832.seq Check: 7341 from: 1 to: 6535
pFN21AB7832 6535 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4345 278
Size: 53 83 186 936 2809 2468
Fragments arranged by size:
2809 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3878 1937
Size: 1941 4594
Acc65I G'GTAC_C
Cuts at: 3856 3856
Size: 6535
AclI AA'CG_TT
Cuts at: 1563 4662 5035 1563
Size: 3099 373 3063
Fragments arranged by size:
3099 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4598 5982 6250 1632
Size: 2966 1384 268 1917
Fragments arranged by size:
2966 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6338
AflIII A'CryG_T
Cuts at: 1203 2921 5434 6411 1203
Size: 1718 2513 977 1327
Fragments arranged by size:
2513 1718 1327 977
AgeI A'CCGG_T
Cuts at: 5366 5366
Size: 6535
AhdI GACnn_n'nnGTC
Cuts at: 5264 5264
Size: 6535
AleI CACnn'nnGTG
Cuts at: 1166 2022 3006 1166
Size: 856 984 4695
Fragments arranged by size:
4695 984 856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6535
AlwNI CAG_nnn'CTG
Cuts at: 2230 4278 5850 2230
Size: 2048 1572 2915
Fragments arranged by size:
2915 2048 1572
ApaLI G'TGCA_C
Cuts at: 2756 4592 5748 2756
Size: 1836 1156 3543
Fragments arranged by size:
3543 1836 1156
ApoI r'AATT_y
Cuts at: 1493 2099 2286 3520 3541 3844 4070 4222 6331
Size: 606 187 1234 21 303 226 152 2109
Cuts at: 6331 1493
Size: 1697
Fragments arranged by size:
2109 1697 1234 606 303 226 187 152
21
AseI AT'TA_AT
Cuts at: 160 1032 5089 160
Size: 872 4057 1606
Fragments arranged by size:
4057 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6535
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 3860 1870
Size: 73 765 1152 4545
Fragments arranged by size:
4545 1152 765 73
BamHI G'GATC_C
Cuts at: 3865 4232 3865
Size: 367 6168
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3856 5311 618
Size: 325 217 627 2069 1455 1842
Fragments arranged by size:
2069 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3462 3854 1391
Size: 2071 392 4072
Fragments arranged by size:
4072 2071 392
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2426 3118 928
Size: 971 527 692 4345
Fragments arranged by size:
4345 971 692 527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4726 4760 1903
Size: 34 50 34 2705 34 3678
Fragments arranged by size:
3678 2705 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3739 4427 5637 3739
Size: 688 1210 4637
Fragments arranged by size:
4637 1210 688
BclI T'GATC_A
Cuts at: 1548 2194 1548
Size: 646 5889
BfrBI ATG'CAT
Cuts at: 2624 3142 2624
Size: 518 6017
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5146 136
Size: 107 122 71 631 605 3474 1525
Fragments arranged by size:
3474 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6530 6530
Size: 6535
BlpI GC'TnA_GC
Cuts at: 3952 3952
Size: 6535
Bme1580I G_kGCm'C
Cuts at: 1790 2760 4596 5752 1790
Size: 970 1836 1156 2573
Fragments arranged by size:
2573 1836 1156 970
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6535
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2598 4266 5224
Size: 416 53 83 399 24 1181 1668 958
Cuts at: 5224 442
Size: 1753
Fragments arranged by size:
1753 1668 1181 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3294 3294
Size: 6535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3339 3580 5195 1239
Size: 2100 241 1615 2579
Fragments arranged by size:
2579 2100 1615 241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3194 3264 4660 5525 5823 3194
Size: 70 1396 865 298 3906
Fragments arranged by size:
3906 1396 865 298 70
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5198 882
Size: 617 3699 2219
Fragments arranged by size:
3699 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4326 493
Size: 711 3122 2702
Fragments arranged by size:
3122 2702 711
BsaBI GATnn'nnATC
Cuts at: 6529 6529
Size: 6535
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4342 4724 5359 275
Size: 53 83 186 936 2809 382 635 1451
Fragments arranged by size:
2809 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4968 5366 5640 5787 6198 4968
Size: 398 274 147 411 5305
Fragments arranged by size:
5305 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3739 3769 1178
Size: 30 165 30 2336 30 3944
Fragments arranged by size:
3944 2336 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6535
BseYI C'CCAG_C
Cuts at: 5738 6234 5738
Size: 496 6039
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3597 3621 1163
Size: 573 1861 24 4077
Fragments arranged by size:
4077 1861 573 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3985 4746 5774 1540
Size: 275 186 1984 761 1028 2301
Fragments arranged by size:
2301 1984 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2031 2760 2809 3462 4596 4681 5752 2031
Size: 729 49 653 1134 85 1071 2814
Fragments arranged by size:
2814 1134 1071 729 653 85 49
BsmI GAATG_Cn'
Cuts at: 2626 4046 4139 2626
Size: 1420 93 5022
Fragments arranged by size:
5022 1420 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2760 2809 3462 3854 4596 4681
Size: 399 241 729 49 653 392 742 85
Cuts at: 4681 5752 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 729 653 399 392 241
85 49
BspEI T'CCGG_A
Cuts at: 6198 6198
Size: 6535
BspHI T'CATG_A
Cuts at: 4424 6154 4424
Size: 1730 4805
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 3890 844
Size: 1210 1836 3489
Fragments arranged by size:
3489 1836 1210
BsrBI CCG'CTC
Cuts at: 1394 1823 4422 1394
Size: 429 2599 3507
Fragments arranged by size:
3507 2599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5030 5204 65
Size: 1183 3782 174 1396
Fragments arranged by size:
3782 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5179 5366 1700
Size: 110 3369 187 2869
Fragments arranged by size:
3369 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6535
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6535
BssSI C'ACGA_G
Cuts at: 4595 5607 4595
Size: 1012 5523
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6535
BstBI TT'CG_AA
Cuts at: 5339 5339
Size: 6535
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6535
BstXI CCAn_nnnn'nTGG
Cuts at: 3008 3008
Size: 6535
BstYI r'GATC_y
Cuts at: 1972 3865 4232 4617 4634 6086 6172 6184 6530
Size: 1893 367 385 17 1452 86 12 346
Cuts at: 6530 1972
Size: 1977
Fragments arranged by size:
1977 1893 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3451 3451
Size: 6535
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5983
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5796
Fragments arranged by size:
5796 683 56
BtsI GCAGTG_nn'
Cuts at: 4051 4845 4865 4051
Size: 794 20 5721
Fragments arranged by size:
5721 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6535
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6500
DraI TTT'AAA
Cuts at: 2110 2259 3546 3839 2110
Size: 149 1287 293 4806
Fragments arranged by size:
4806 1287 293 149
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5542 809
Size: 507 4226 1802
Fragments arranged by size:
4226 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3982 4871 6500
Size: 54 1425 50 275 18 2152 889 1629
Cuts at: 6500 8
Size: 43
Fragments arranged by size:
2152 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3982 1537
Size: 275 2170 4090
Fragments arranged by size:
4090 2170 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 2871 3705 4465 1353
Size: 960 558 834 760 3423
Fragments arranged by size:
3423 960 834 760 558
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2876 5122 5496 5642 387
Size: 1064 138 1287 2246 374 146 1280
Fragments arranged by size:
2246 1287 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3460 3460
Size: 6535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3339 3580 4598 5195 5982 6250 1239
Size: 393 1707 241 1018 597 787 268 1524
Fragments arranged by size:
1707 1524 1018 787 597 393 268 241
EcoNI CCTnn'n_nnAGG
Cuts at: 2508 2508
Size: 6535
EcoRI G'AATT_C
Cuts at: 2099 3844 4222 2099
Size: 1745 378 4412
Fragments arranged by size:
4412 1745 378
EcoRV GAT'ATC
Cuts at: 2391 2391
Size: 6535
FspI TGC'GCA
Cuts at: 5041 5041
Size: 6535
HaeII r_GCGC'y
Cuts at: 1678 5682 1678
Size: 4004 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 2702 2734 3486
Size: 32 135 32 1037 32 61 32 752
Cuts at: 3486 3518 3737 3769 5176 5208 5250 5282 1373
Size: 32 219 32 1407 32 42 32 2626
Fragments arranged by size:
2626 1407 1037 752 219 135 61 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2715 3783 3879 4125 669
Size: 988 281 777 1068 96 246 3079
Fragments arranged by size:
3079 1068 988 777 281 246 96
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6535
HpaI GTT'AAC
Cuts at: 4125 4125
Size: 6535
KpnI G_GTAC'C
Cuts at: 3860 3860
Size: 6535
MfeI C'AATT_G
Cuts at: 4134 4134
Size: 6535
MluI A'CGCG_T
Cuts at: 6411 6411
Size: 6535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2214 2252 5649 5833 1305
Size: 909 38 3397 184 2007
Fragments arranged by size:
3397 2007 909 184 38
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4713
Fragments arranged by size:
4713 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2022 2121 3006 3063 3079 3147 3402
Size: 648 856 99 885 57 16 68 255
Cuts at: 3402 3418 3573 4493 4852 5011 518
Size: 16 155 920 359 159 2042
Fragments arranged by size:
2042 920 885 856 648 359 255 159
155 99 68 57 16 16
MspA1I CmG'CkG
Cuts at: 1606 2056 3037 3951 4628 5776 6021 1606
Size: 450 981 914 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 914 677 450 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5983
NdeI CA'TA_TG
Cuts at: 387 3247 3419 387
Size: 2860 172 3503
Fragments arranged by size:
3503 2860 172
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6535
NotI GC'GGCC_GC
Cuts at: 3982 3982
Size: 6535
NsiI A_TGCA'T
Cuts at: 2626 3144 2626
Size: 518 6017
NspI r_CATG'y
Cuts at: 1132 1229 2925 3050 3142 3730 3893 5438 1132
Size: 97 1696 125 92 588 163 1545 2229
Fragments arranged by size:
2229 1696 1545 588 163 125 97 92
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6535
PciI A'CATG_T
Cuts at: 2921 5434 2921
Size: 2513 4022
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6535
PmeI GTTT'AAAC
Cuts at: 3839 3839
Size: 6535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4604 4636 6117 6149 4604
Size: 32 1481 32 4990
Fragments arranged by size:
4990 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6535
PsiI TTA'TAA
Cuts at: 2454 4105 2454
Size: 1651 4884
PspXI vC'TCGA_Gb
Cuts at: 2708 2708
Size: 6535
PstI C_TGCA'G
Cuts at: 830 3887 830
Size: 3057 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6535
PvuII CAG'CTG
Cuts at: 3037 3037
Size: 6535
SacI G_AGCT'C
Cuts at: 3462 3462
Size: 6535
SalI G'TCGA_C
Cuts at: 1936 3877 1936
Size: 1941 4594
SapI GCTCTTCn'nnn_
Cuts at: 2871 3705 2871
Size: 834 5701
SbfI CC_TGCA'GG
Cuts at: 3887 3887
Size: 6535
ScaI AGT'ACT
Cuts at: 4783 6495 4783
Size: 1712 4823
SfcI C'TryA_G
Cuts at: 826 1045 2161 2382 2481 2667 3883 5018 5699
Size: 219 1116 221 99 186 1216 1135 681
Cuts at: 5699 5890 826
Size: 191 1471
Fragments arranged by size:
1471 1216 1135 1116 681 221 219 191
186 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6535
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6535
SmaI CCC'GGG
Cuts at: 1872 3862 1872
Size: 1990 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2708 3209 3279 4639 5540 5802
Size: 197 926 765 501 70 1360 901 262
Cuts at: 5802 820
Size: 1553
Fragments arranged by size:
1553 1360 926 901 765 501 262 197
70
SnaBI TAC'GTA
Cuts at: 493 4326 493
Size: 3833 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6535
SphI G_CATG'C
Cuts at: 1132 3142 3893 1132
Size: 2010 751 3774
Fragments arranged by size:
3774 2010 751
SspI AAT'ATT
Cuts at: 5 52 4459 5
Size: 47 4407 2081
Fragments arranged by size:
4407 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6535
StyI C'CwwG_G
Cuts at: 513 1065 2898 3974 513
Size: 552 1833 1076 3074
Fragments arranged by size:
3074 1833 1076 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3465 4562 4579 4732 4917 5362 325
Size: 1255 1885 1097 17 153 185 445 1498
Fragments arranged by size:
1885 1498 1255 1097 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4781 6493 96
Size: 274 80 33 51 1443 2804 1712 138
Fragments arranged by size:
2804 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3217 3249 3461 3493 6495 6527 3217
Size: 32 212 32 3002 32 3225
Fragments arranged by size:
3225 3002 212 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6535
XbaI T'CTAG_A
Cuts at: 3589 3871 3589
Size: 282 6253
XhoI C'TCGA_G
Cuts at: 1943 2708 1943
Size: 765 5770
XmaI C'CCGG_G
Cuts at: 1870 3860 1870
Size: 1990 4545
XmnI GAAnn'nnTTC
Cuts at: 2170 2558 4664 2170
Size: 388 2106 4041
Fragments arranged by size:
4041 2106 388
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4343 276
Size: 53 83 186 936 2809 2468
Fragments arranged by size:
2809 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoO109I FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI