Restriction map for
pFN21AB7827
(Circular) (Six-base) MAPSORT of: pFN21AB7827.seq Check: 9097 from: 1 to: 6319
pFN21AB7827 6319 bp
With 182 enzymes: SgfI *
March 28, 2012 12:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2110 2458 3130 4382 5766 6034 1632
Size: 478 348 672 1252 1384 268 1917
Fragments arranged by size:
1917 1384 1252 672 478 348 268
AflII C'TTAA_G
Cuts at: 820 1017 2828 2912 820
Size: 197 1811 84 4227
Fragments arranged by size:
4227 1811 197 84
AflIII A'CryG_T
Cuts at: 1203 2534 2963 5218 6195 1203
Size: 1331 429 2255 977 1327
Fragments arranged by size:
2255 1331 1327 977 429
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 3273 1166
Size: 2107 4212
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6319
AlwNI CAG_nnn'CTG
Cuts at: 4062 5634 4062
Size: 1572 4747
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 2233 2639 3238 3628 3854 4006 6115 1493
Size: 740 406 599 390 226 152 2109 1697
Fragments arranged by size:
2109 1697 740 599 406 390 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 3644 1870
Size: 73 1701 4545
Fragments arranged by size:
4545 1701 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3640 5095 618
Size: 325 217 627 1853 1455 1842
Fragments arranged by size:
1853 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3638 1391
Size: 2247 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2044 3243 928
Size: 971 145 1199 4004
Fragments arranged by size:
4004 1199 971 145
BbvCI CC'TCA_GC
Cuts at: 2502 2502
Size: 6319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4930 136
Size: 107 122 71 631 605 3258 1525
Fragments arranged by size:
3258 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 3736 3736
Size: 6319
Bme1580I G_kGCm'C
Cuts at: 1790 4380 5536 1790
Size: 2590 1156 2573
Fragments arranged by size:
2590 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2862 4050 5008
Size: 416 53 83 399 24 1445 1188 958
Cuts at: 5008 442
Size: 1753
Fragments arranged by size:
1753 1445 1188 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2627
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2706 4979 1239
Size: 899 568 2273 2579
Fragments arranged by size:
2579 2273 899 568
Bpu10I CC'TnA_GC
Cuts at: 2502 2502
Size: 6319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3394 4444 5309 5607 3394
Size: 1050 865 298 4106
Fragments arranged by size:
4106 1050 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2349 3358 4982 882
Size: 617 850 1009 1624 2219
Fragments arranged by size:
2219 1624 1009 850 617
BsaAI yAC'GTr
Cuts at: 493 1204 2599 4110 493
Size: 711 1395 1511 2702
Fragments arranged by size:
2702 1511 1395 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4126 4508 5143 275
Size: 53 83 186 936 2593 382 635 1451
Fragments arranged by size:
2593 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4752 5150 5424 5571 5982 4752
Size: 398 274 147 411 5089
Fragments arranged by size:
5089 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6094
Fragments arranged by size:
6094 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2078 3311 1186
Size: 889 3 1233 4194
Fragments arranged by size:
4194 1233 889 3
BseYI C'CCAG_C
Cuts at: 2068 2823 5522 6018 2068
Size: 755 2699 496 2369
Fragments arranged by size:
2699 2369 755 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5746
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2287 4380 4465 5536 2287
Size: 2093 85 1071 3070
Fragments arranged by size:
3070 2093 1071 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
BsmBI CGTCTCn'nnnn_
Cuts at: 3553 3553
Size: 6319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2287 3638 4380 4465 5536 1391
Size: 399 497 1351 742 85 1071 2174
Fragments arranged by size:
2174 1351 1071 742 497 399 85
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 2815 4208 5938 2815
Size: 1393 1730 3196
Fragments arranged by size:
3196 1730 1393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2142 3674 844
Size: 1298 1532 3489
Fragments arranged by size:
3489 1532 1298
BsrBI CCG'CTC
Cuts at: 1394 1823 4206 1394
Size: 429 2383 3507
Fragments arranged by size:
3507 2383 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2717 3004 4814 4988 65
Size: 1183 1469 287 1810 174 1396
Fragments arranged by size:
1810 1469 1396 1183 287 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4963 5150 1700
Size: 110 3153 187 2869
Fragments arranged by size:
3153 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3484 96
Size: 3388 2931
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6319
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 2247 3263 3649 4016 4401 4418 5870 5956
Size: 275 1016 386 367 385 17 1452 86
Cuts at: 5956 5968 6314 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1016 386 385 367 346 275
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2183 2183
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 2925 513
Size: 552 1860 3907
Fragments arranged by size:
3907 1860 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2031 3053 3436 3835 4629 4649 2031
Size: 1022 383 399 794 20 3701
Fragments arranged by size:
3701 1022 794 399 383 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3766 4655 6284
Size: 54 1425 50 275 18 1936 889 1629
Cuts at: 6284 8
Size: 43
Fragments arranged by size:
1936 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 2514 2580 3134 4249 1353
Size: 933 228 66 554 1115 3423
Fragments arranged by size:
3423 1115 933 554 228 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2138 2458 2706 3130 4382 4979
Size: 393 478 28 320 248 424 1252 597
Cuts at: 4979 5766 6034 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1252 787 597 478 424 393 320
268 248 28
EcoRI G'AATT_C
Cuts at: 2233 3628 4006 2233
Size: 1395 378 4546
Fragments arranged by size:
4546 1395 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3592 3592
Size: 6319
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 3577 5466 1678
Size: 1899 1889 2531
Fragments arranged by size:
2531 1899 1889
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2232 2249 2264 2281 2381
Size: 32 135 32 660 17 15 17 100
Cuts at: 2381 2413 2965 2997 4960 4992 5034 5066 1373
Size: 32 552 32 1963 32 42 32 2626
Fragments arranged by size:
2626 1963 660 552 135 100 42 32
32 32 32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3199 748
Size: 2451 3868
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 5433 5617 1305
Size: 936 3192 184 2007
Fragments arranged by size:
3192 2007 936 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4497
Fragments arranged by size:
4497 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2701 2869 2953 3273 3289 3457 3541
Size: 648 1535 168 84 320 16 168 84
Cuts at: 3541 4277 4636 4795 518
Size: 736 359 159 2042
Fragments arranged by size:
2042 1535 736 648 359 320 168 168
159 84 84 16
MspA1I CmG'CkG
Cuts at: 1606 3735 4412 5560 5805 1606
Size: 2129 677 1148 245 2120
Fragments arranged by size:
2129 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2925 513
Size: 552 1860 3907
Fragments arranged by size:
3907 1860 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2538 2799 2967 3677 5222 1132
Size: 97 1309 261 168 710 1545 2229
Fragments arranged by size:
2229 1545 1309 710 261 168 97
PasI CC'CwG_GG
Cuts at: 1348 2449 1348
Size: 1101 5218
PciI A'CATG_T
Cuts at: 2534 2963 5218 2534
Size: 429 2255 3635
Fragments arranged by size:
3635 2255 429
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2926 1108
Size: 1818 4501
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PmlI CAC'GTG
Cuts at: 2599 2599
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2123 2155 4388 4420 5901 5933 2123
Size: 32 2233 32 1481 32 2509
Fragments arranged by size:
2509 2233 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3084 3372 3889 3084
Size: 288 517 5514
Fragments arranged by size:
5514 517 288
PstI C_TGCA'G
Cuts at: 830 3671 830
Size: 2841 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SapI GCTCTTCn'nnn_
Cuts at: 2580 3134 2580
Size: 554 5765
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SexAI A'CCwGG_T
Cuts at: 2339 2339
Size: 6319
SfcI C'TryA_G
Cuts at: 826 1045 3520 3667 4802 5483 5674 826
Size: 219 2475 147 1135 681 191 1471
Fragments arranged by size:
2475 1471 1135 681 219 191 147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3646 1872
Size: 1774 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2828 2912 3409 4423 5324 5586
Size: 197 926 885 84 497 1014 901 262
Cuts at: 5586 820
Size: 1553
Fragments arranged by size:
1553 1014 926 901 885 497 262 197
84
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 2807 1517
Size: 1290 5029
StyI C'CwwG_G
Cuts at: 513 1065 2925 3758 513
Size: 552 1860 833 3074
Fragments arranged by size:
3074 1860 833 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4346 4363 4516 4701 5146 325
Size: 1255 2766 17 153 185 445 1498
Fragments arranged by size:
2766 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3484 4565 6277
Size: 274 80 33 51 1443 1507 1081 1712
Cuts at: 6277 96
Size: 138
Fragments arranged by size:
1712 1507 1443 1081 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 3644 1870
Size: 1774 4545
XmnI GAAnn'nnTTC
Cuts at: 2696 2723 2807 2864 3143 4448 2696
Size: 27 84 57 279 1305 4567
Fragments arranged by size:
4567 1305 279 84 57 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI
XcmI