Restriction map for
pFN21AB7824
(Circular) (Six-base) MAPSORT of: pFN21AB7824.seq Check: 9830 from: 1 to: 6517
pFN21AB7824 6517 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4327 278
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2909 3552 3860 1937
Size: 972 643 308 4594
Fragments arranged by size:
4594 972 643 308
Acc65I G'GTAC_C
Cuts at: 3838 3838
Size: 6517
AclI AA'CG_TT
Cuts at: 1563 4644 5017 1563
Size: 3081 373 3063
Fragments arranged by size:
3081 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2485 2610 2887 3139 4580 5964 6232 1632
Size: 853 125 277 252 1441 1384 268 1917
Fragments arranged by size:
1917 1441 1384 853 277 268 252 125
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6320
AflIII A'CryG_T
Cuts at: 1203 5416 6393 1203
Size: 4213 977 1327
Fragments arranged by size:
4213 1327 977
AgeI A'CCGG_T
Cuts at: 5348 5348
Size: 6517
AhdI GACnn_n'nnGTC
Cuts at: 5246 5246
Size: 6517
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6517
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6517
AlwNI CAG_nnn'CTG
Cuts at: 2633 4260 5832 2633
Size: 1627 1572 3318
Fragments arranged by size:
3318 1627 1572
ApaLI G'TGCA_C
Cuts at: 4574 5730 4574
Size: 1156 5361
ApoI r'AATT_y
Cuts at: 1493 2276 2484 3826 4052 4204 6313 1493
Size: 783 208 1342 226 152 2109 1697
Fragments arranged by size:
2109 1697 1342 783 226 208 152
AseI AT'TA_AT
Cuts at: 160 1032 5071 160
Size: 872 4039 1606
Fragments arranged by size:
4039 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
AvaI C'yCGr_G
Cuts at: 1870 1943 2860 3842 1870
Size: 73 917 982 4545
Fragments arranged by size:
4545 982 917 73
BamHI G'GATC_C
Cuts at: 3847 4214 3847
Size: 367 6150
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3264 3838 5293 618
Size: 325 217 627 1477 574 1455 1842
Fragments arranged by size:
1842 1477 1455 627 574 325 217
BanII G_rGCy'C
Cuts at: 1391 3107 3836 1391
Size: 1716 729 4072
Fragments arranged by size:
4072 1716 729
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3805 928
Size: 971 1906 3640
Fragments arranged by size:
3640 1906 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3221 3255 4708 4742 1903
Size: 34 1284 34 1453 34 3678
Fragments arranged by size:
3678 1453 1284 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4409 5619 4409
Size: 1210 5307
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6517
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5128 136
Size: 107 122 71 631 605 3456 1525
Fragments arranged by size:
3456 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2361 6512 2361
Size: 4151 2366
BlpI GC'TnA_GC
Cuts at: 3934 3934
Size: 6517
Bme1580I G_kGCm'C
Cuts at: 1790 2717 3234 3305 3389 3473 4578 5734 1790
Size: 927 517 71 84 84 1105 1156 2573
Fragments arranged by size:
2573 1156 1105 927 517 84 84 71
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6517
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2516 2672 3301
Size: 416 53 83 399 24 1099 156 629
Cuts at: 3301 4248 5206 442
Size: 947 958 1753
Fragments arranged by size:
1753 1099 958 947 629 416 399 156
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6517
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2121 2121
Size: 6517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2222 2655 3077 5177 1239
Size: 917 66 433 422 2100 2579
Fragments arranged by size:
2579 2100 917 433 422 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3158 4642 5507 5805 3158
Size: 1484 865 298 3870
Fragments arranged by size:
3870 1484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2161 5180 882
Size: 617 662 3019 2219
Fragments arranged by size:
3019 2219 662 617
BsaAI yAC'GTr
Cuts at: 493 1204 2648 3550 4308 493
Size: 711 1444 902 758 2702
Fragments arranged by size:
2702 1444 902 758 711
BsaBI GATnn'nnATC
Cuts at: 6511 6511
Size: 6517
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2812 4324 4706 5341
Size: 53 83 186 936 1279 1512 382 635
Cuts at: 5341 275
Size: 1451
Fragments arranged by size:
1512 1451 1279 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4950 5348 5622 5769 6180 4950
Size: 398 274 147 411 5287
Fragments arranged by size:
5287 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2001 2031 2858 2888 3698
Size: 30 165 30 598 30 827 30 810
Cuts at: 3698 3728 1178
Size: 30 3967
Fragments arranged by size:
3967 827 810 598 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 2315 3047 3080 3161 3497 3551 3554
Size: 1057 72 732 33 81 336 54 3
Cuts at: 3554 1186
Size: 4149
Fragments arranged by size:
4149 1057 732 336 81 72 54 33
3
BseYI C'CCAG_C
Cuts at: 5720 6216 5720
Size: 496 6021
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2902 1163
Size: 573 1166 4778
Fragments arranged by size:
4778 1166 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3967 4728 5756 1540
Size: 275 186 1966 761 1028 2301
Fragments arranged by size:
2301 1966 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3107 4578 4663 5734 3107
Size: 1471 85 1071 3890
Fragments arranged by size:
3890 1471 1071 85
BsmI GAATG_Cn'
Cuts at: 2726 4028 4121 2726
Size: 1302 93 5122
Fragments arranged by size:
5122 1302 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2717 3107 3234 3305 3389 3473 3836
Size: 399 927 390 127 71 84 84 363
Cuts at: 3836 4578 4663 5734 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 927 742 399 390 363 127
85 84 84 71
BspEI T'CCGG_A
Cuts at: 6180 6180
Size: 6517
BspHI T'CATG_A
Cuts at: 4406 6136 4406
Size: 1730 4787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3872 844
Size: 3028 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4404 1394
Size: 429 2581 3507
Fragments arranged by size:
3507 2581 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5012 5186 65
Size: 1183 3764 174 1396
Fragments arranged by size:
3764 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5161 5348 1700
Size: 110 3351 187 2869
Fragments arranged by size:
3351 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2118 96
Size: 2022 4495
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6517
BssSI C'ACGA_G
Cuts at: 4577 5589 4577
Size: 1012 5505
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6517
BstBI TT'CG_AA
Cuts at: 5321 5321
Size: 6517
BstEII G'GTnAC_C
Cuts at: 1184 2516 3334 3418 1184
Size: 1332 818 84 4283
Fragments arranged by size:
4283 1332 818 84
BstXI CCAn_nnnn'nTGG
Cuts at: 2529 2529
Size: 6517
BstYI r'GATC_y
Cuts at: 1972 2361 3847 4214 4599 4616 6068 6154 6166
Size: 389 1486 367 385 17 1452 86 12
Cuts at: 6166 6512 1972
Size: 346 1977
Fragments arranged by size:
1977 1486 1452 389 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2910 2910
Size: 6517
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5965
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3654 487
Size: 683 56 2428 3350
Fragments arranged by size:
3350 2428 683 56
BtsI GCAGTG_nn'
Cuts at: 4033 4827 4847 4033
Size: 794 20 5703
Fragments arranged by size:
5703 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6517
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6482
DraI TTT'AAA
Cuts at: 3821 3821
Size: 6517
DraIII CAC_nnn'GTG
Cuts at: 2302 2302
Size: 6517
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5524 809
Size: 507 4208 1802
Fragments arranged by size:
4208 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2347 3577 3964
Size: 54 1425 50 275 18 517 1230 387
Cuts at: 3964 4853 6482 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1230 889 517 387 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3964 1537
Size: 275 2152 4090
Fragments arranged by size:
4090 2152 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4447 1353
Size: 3094 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5104 5478 5624 387
Size: 1064 138 3515 374 146 1280
Fragments arranged by size:
3515 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3105 3105
Size: 6517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2222 2485 2610 2655 2887 3077
Size: 393 524 66 263 125 45 232 190
Cuts at: 3077 3139 4580 5177 5964 6232 1239
Size: 62 1441 597 787 268 1524
Fragments arranged by size:
1524 1441 787 597 524 393 268 263
232 190 125 66 62 45
EcoO109I rG'GnC_Cy
Cuts at: 2112 2450 2955 3123 2112
Size: 338 505 168 5506
Fragments arranged by size:
5506 505 338 168
EcoRI G'AATT_C
Cuts at: 2276 3826 4204 2276
Size: 1550 378 4589
Fragments arranged by size:
4589 1550 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3775 3775
Size: 6517
FspI TGC'GCA
Cuts at: 5023 5023
Size: 6517
HaeII r_GCGC'y
Cuts at: 1678 5664 1678
Size: 3986 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5158 5190 5232 5264 1373
Size: 32 135 32 3586 32 42 32 2626
Fragments arranged by size:
3586 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3861 4107 669
Size: 988 281 1923 246 3079
Fragments arranged by size:
3079 1923 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6517
HpaI GTT'AAC
Cuts at: 4107 4107
Size: 6517
KpnI G_GTAC'C
Cuts at: 3842 3842
Size: 6517
MfeI C'AATT_G
Cuts at: 4116 4116
Size: 6517
MluI A'CGCG_T
Cuts at: 6393 6393
Size: 6517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2419 3706 5631 5815 1305
Size: 1114 1287 1925 184 2007
Fragments arranged by size:
2007 1925 1287 1114 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2349 3579 10
Size: 54 1425 343 517 1230 2948
Fragments arranged by size:
2948 1425 1230 517 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2514 2794 4475 4834 4993 518
Size: 648 1348 280 1681 359 159 2042
Fragments arranged by size:
2042 1681 1348 648 359 280 159
MspA1I CmG'CkG
Cuts at: 1606 2200 3691 3933 4610 5758 6003 1606
Size: 594 1491 242 677 1148 245 2120
Fragments arranged by size:
2120 1491 1148 677 594 245 242
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5965
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6517
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6517
NotI GC'GGCC_GC
Cuts at: 3964 3964
Size: 6517
NspI r_CATG'y
Cuts at: 1132 1229 3875 5420 1132
Size: 97 2646 1545 2229
Fragments arranged by size:
2646 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2161 1348
Size: 813 5704
PciI A'CATG_T
Cuts at: 5416 5416
Size: 6517
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2302 1108
Size: 1194 5323
PfoI T'CCnGG_A
Cuts at: 2052 2052
Size: 6517
PmeI GTTT'AAAC
Cuts at: 3821 3821
Size: 6517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4586 4618 6099 6131 4586
Size: 32 1481 32 4972
Fragments arranged by size:
4972 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2112 2112
Size: 6517
PshAI GACnn'nnGTC
Cuts at: 1613 2253 1613
Size: 640 5877
PsiI TTA'TAA
Cuts at: 4087 4087
Size: 6517
PstI C_TGCA'G
Cuts at: 830 2383 3725 3869 830
Size: 1553 1342 144 3478
Fragments arranged by size:
3478 1553 1342 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6517
PvuII CAG'CTG
Cuts at: 2200 2200
Size: 6517
SacI G_AGCT'C
Cuts at: 3107 3107
Size: 6517
SalI G'TCGA_C
Cuts at: 1936 3859 1936
Size: 1923 4594
SbfI CC_TGCA'GG
Cuts at: 3869 3869
Size: 6517
ScaI AGT'ACT
Cuts at: 4765 6477 4765
Size: 1712 4805
SexAI A'CCwGG_T
Cuts at: 2298 3183 2298
Size: 885 5632
SfcI C'TryA_G
Cuts at: 826 1045 2143 2379 2656 3721 3865 5000 5681
Size: 219 1098 236 277 1065 144 1135 681
Cuts at: 5681 5872 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1098 1065 681 277 236 219
191 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6517
SmaI CCC'GGG
Cuts at: 1872 3844 1872
Size: 1972 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3137 4621 5522 5784 820
Size: 197 926 1194 1484 901 262 1553
Fragments arranged by size:
1553 1484 1194 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3550 4308 493
Size: 3057 758 2702
Fragments arranged by size:
3057 2702 758
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6517
SphI G_CATG'C
Cuts at: 1132 3875 1132
Size: 2743 3774
SspI AAT'ATT
Cuts at: 5 52 4441 5
Size: 47 4389 2081
Fragments arranged by size:
4389 2081 47
StuI AGG'CCT
Cuts at: 1517 2831 1517
Size: 1314 5203
StyI C'CwwG_G
Cuts at: 513 1065 2219 3956 513
Size: 552 1154 1737 3074
Fragments arranged by size:
3074 1737 1154 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2090 4544 4561 4714 4899 5344 325
Size: 1255 510 2454 17 153 185 445 1498
Fragments arranged by size:
2454 1498 1255 510 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 4763 6475
Size: 274 80 33 51 1443 141 2645 1712
Cuts at: 6475 96
Size: 138
Fragments arranged by size:
2645 1712 1443 274 141 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2001 2033 2349 2381 6477 6509 2001
Size: 32 316 32 4096 32 2009
Fragments arranged by size:
4096 2009 316 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3781 1316
Size: 2465 4052
XbaI T'CTAG_A
Cuts at: 3853 3853
Size: 6517
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6517
XmaI C'CCGG_G
Cuts at: 1870 3842 1870
Size: 1972 4545
XmnI GAAnn'nnTTC
Cuts at: 2241 3041 4646 2241
Size: 800 1605 4112
Fragments arranged by size:
4112 1605 800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4325 276
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI