Restriction map for
pFN21AB7821
(Circular) (Six-base) MAPSORT of: pFN21AB7821.seq Check: 3579 from: 1 to: 6313
pFN21AB7821 6313 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 3656 1937
Size: 1401 318 4594
Fragments arranged by size:
4594 1401 318
Acc65I G'GTAC_C
Cuts at: 3634 3634
Size: 6313
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2724 3546 3552 4376 5760 6028 1632
Size: 1092 822 6 824 1384 268 1917
Fragments arranged by size:
1917 1384 1092 824 822 268 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 5212 6189 1203
Size: 4009 977 1327
Fragments arranged by size:
4009 1327 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 5042 5042
Size: 6313
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2151 1184
Size: 967 5346
AlwNI CAG_nnn'CTG
Cuts at: 4056 5628 4056
Size: 1572 4741
ApaLI G'TGCA_C
Cuts at: 4370 5526 4370
Size: 1156 5157
ApoI r'AATT_y
Cuts at: 1493 2145 2273 2364 3622 3848 4000 6109 1493
Size: 652 128 91 1258 226 152 2109 1697
Fragments arranged by size:
2109 1697 1258 652 226 152 128 91
AseI AT'TA_AT
Cuts at: 160 1032 4867 160
Size: 872 3835 1606
Fragments arranged by size:
3835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 3638 1870
Size: 73 1695 4545
Fragments arranged by size:
4545 1695 73
BamHI G'GATC_C
Cuts at: 3643 4010 3643
Size: 367 5946
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3634 5089 618
Size: 325 217 627 1847 1455 1842
Fragments arranged by size:
1847 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2626 3632 1391
Size: 1235 1006 4072
Fragments arranged by size:
4072 1235 1006
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3151 928
Size: 971 1252 4090
Fragments arranged by size:
4090 1252 971
BbvCI CC'TCA_GC
Cuts at: 2036 2036
Size: 6313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4504 4538 1903
Size: 34 2567 34 3678
Fragments arranged by size:
3678 2567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4205 5415 4205
Size: 1210 5103
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6313
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 4924 136
Size: 107 122 71 631 605 428 2824 1525
Fragments arranged by size:
2824 1525 631 605 428 122 107 71
BglII A'GATC_T
Cuts at: 3166 6308 3166
Size: 3142 3171
BlpI GC'TnA_GC
Cuts at: 3730 3730
Size: 6313
Bme1580I G_kGCm'C
Cuts at: 1790 4374 5530 1790
Size: 2584 1156 2573
Fragments arranged by size:
2584 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2783 4044 5002
Size: 416 53 83 399 24 1366 1261 958
Cuts at: 5002 442
Size: 1753
Fragments arranged by size:
1753 1366 1261 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 3533 4973 1239
Size: 871 1423 1440 2579
Fragments arranged by size:
2579 1440 1423 871
Bpu10I CC'TnA_GC
Cuts at: 2036 2036
Size: 6313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4438 5303 5601 4438
Size: 865 298 5150
Fragments arranged by size:
5150 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4976 882
Size: 617 3477 2219
Fragments arranged by size:
3477 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4104 493
Size: 711 2900 2702
Fragments arranged by size:
2900 2702 711
BsaBI GATnn'nnATC
Cuts at: 2673 6307 2673
Size: 3634 2679
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4120 4502 5137 275
Size: 53 83 186 936 2587 382 635 1451
Fragments arranged by size:
2587 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4746 5144 5418 5565 5976 4746
Size: 398 274 147 411 5083
Fragments arranged by size:
5083 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6088
Fragments arranged by size:
6088 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6313
BseYI C'CCAG_C
Cuts at: 5516 6012 5516
Size: 496 5817
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3218 3561 1163
Size: 573 1482 343 3915
Fragments arranged by size:
3915 1482 573 343
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2404 4374 4459 5530 2404
Size: 1970 85 1071 3187
Fragments arranged by size:
3187 1970 1071 85
BsmI GAATG_Cn'
Cuts at: 3359 3824 3917 3359
Size: 465 93 5755
Fragments arranged by size:
5755 465 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2404 2626 3632 4374 4459 5530 1391
Size: 399 614 222 1006 742 85 1071 2174
Fragments arranged by size:
2174 1071 1006 742 614 399 222 85
BspEI T'CCGG_A
Cuts at: 5976 5976
Size: 6313
BspHI T'CATG_A
Cuts at: 4202 5932 4202
Size: 1730 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3668 844
Size: 2824 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4200 1394
Size: 429 2377 3507
Fragments arranged by size:
3507 2377 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4808 4982 65
Size: 1183 3560 174 1396
Fragments arranged by size:
3560 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2026 4957 5144 1700
Size: 110 216 2931 187 2869
Fragments arranged by size:
2931 2869 216 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 4373 5385 4373
Size: 1012 5301
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2507 794
Size: 1713 4600
BstBI TT'CG_AA
Cuts at: 2018 2530 5117 2018
Size: 512 2587 3214
Fragments arranged by size:
3214 2587 512
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstXI CCAn_nnnn'nTGG
Cuts at: 2993 2993
Size: 6313
BstYI r'GATC_y
Cuts at: 1972 3019 3166 3643 4010 4395 4412 5864 5950
Size: 1047 147 477 367 385 17 1452 86
Cuts at: 5950 5962 6308 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1047 477 385 367 346 147
86 17 12
BstZ17I GTA'TAC
Cuts at: 3339 3339
Size: 6313
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5761
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 2956 3829 4623 4643 2956
Size: 873 794 20 4626
Fragments arranged by size:
4626 873 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6278
DraI TTT'AAA
Cuts at: 2586 2712 3617 2586
Size: 126 905 5282
Fragments arranged by size:
5282 905 126
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5320 809
Size: 507 4004 1802
Fragments arranged by size:
4004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2101 3760 4649
Size: 54 1425 50 275 18 271 1659 889
Cuts at: 4649 6278 8
Size: 1629 43
Fragments arranged by size:
1659 1629 1425 889 275 271 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3285 4243 1353
Size: 1932 958 3423
Fragments arranged by size:
3423 1932 958
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4900 5274 5420 387
Size: 1064 138 3311 374 146 1280
Fragments arranged by size:
3311 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2724 3533 3546 3552 4376 4973
Size: 393 478 614 809 13 6 824 597
Cuts at: 4973 5760 6028 1239
Size: 787 268 1524
Fragments arranged by size:
1524 824 809 787 614 597 478 393
268 13 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2477 2477
Size: 6313
EcoRI G'AATT_C
Cuts at: 3622 4000 3622
Size: 378 5935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2165 2165
Size: 6313
FspI TGC'GCA
Cuts at: 4819 4819
Size: 6313
HaeII r_GCGC'y
Cuts at: 1678 5460 1678
Size: 3782 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 2804 2836 4954
Size: 32 135 32 881 32 319 32 2118
Cuts at: 4954 4986 5028 5060 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2118 881 319 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3657 3903 669
Size: 988 281 1719 246 3079
Fragments arranged by size:
3079 1719 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2182 3500 748
Size: 1434 1318 3561
Fragments arranged by size:
3561 1434 1318
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KpnI G_GTAC'C
Cuts at: 3638 3638
Size: 6313
MfeI C'AATT_G
Cuts at: 3912 3912
Size: 6313
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2295 2856 3066 5427 5611 1305
Size: 990 561 210 2361 184 2007
Fragments arranged by size:
2361 2007 990 561 210 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2103 10
Size: 54 1425 343 271 4220
Fragments arranged by size:
4220 1425 343 271 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4271 4630 4789 518
Size: 648 3105 359 159 2042
Fragments arranged by size:
3105 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3729 4406 5554 5799 1606
Size: 2123 677 1148 245 2120
Fragments arranged by size:
2123 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2028 2028
Size: 6313
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5761
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NgoMIV G'CCGG_C
Cuts at: 2026 2026
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 2510 3671 5216 1132
Size: 97 1281 1161 1545 2229
Fragments arranged by size:
2229 1545 1281 1161 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6313
PciI A'CATG_T
Cuts at: 5212 5212
Size: 6313
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6313
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6313
PsiI TTA'TAA
Cuts at: 2393 3883 2393
Size: 1490 4823
PstI C_TGCA'G
Cuts at: 830 3113 3665 830
Size: 2283 552 3478
Fragments arranged by size:
3478 2283 552
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SfcI C'TryA_G
Cuts at: 826 1045 2268 2836 3109 3432 3661 4796 5477
Size: 219 1223 568 273 323 229 1135 681
Cuts at: 5477 5668 826
Size: 191 1471
Fragments arranged by size:
1471 1223 1135 681 568 323 273 229
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 3640 1872
Size: 1768 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4417 5318 5580 820
Size: 197 926 2474 901 262 1553
Fragments arranged by size:
2474 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4104 493
Size: 3611 2702
SpeI A'CTAG_T
Cuts at: 152 3188 152
Size: 3036 3277
SphI G_CATG'C
Cuts at: 1132 2510 3671 1132
Size: 1378 1161 3774
Fragments arranged by size:
3774 1378 1161
SspI AAT'ATT
Cuts at: 5 52 2159 4237 5
Size: 47 2107 2078 2081
Fragments arranged by size:
2107 2081 2078 47
StuI AGG'CCT
Cuts at: 1517 2599 1517
Size: 1082 5231
StyI C'CwwG_G
Cuts at: 513 1065 2717 3752 513
Size: 552 1652 1035 3074
Fragments arranged by size:
3074 1652 1035 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4340 4357 4510 4695 5140 325
Size: 1255 2760 17 153 185 445 1498
Fragments arranged by size:
2760 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2224 3401 4559
Size: 274 80 33 51 1443 247 1177 1158
Cuts at: 4559 6271 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1177 1158 274 247 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6273 6305 6273
Size: 32 6281
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6313
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6313
XmaI C'CCGG_G
Cuts at: 1870 3638 1870
Size: 1768 4545
XmnI GAAnn'nnTTC
Cuts at: 2148 4442 2148
Size: 2294 4019
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI