Restriction map for
pFN21AB7820
(Circular) (Six-base) MAPSORT of: pFN21AB7820.seq Check: 3299 from: 1 to: 6502
pFN21AB7820 6502 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2931 2931
Size: 6502
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4312 278
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2217 3845 1937
Size: 280 1628 4594
Fragments arranged by size:
4594 1628 280
Acc65I G'GTAC_C
Cuts at: 3092 3250 3823 3092
Size: 158 573 5771
Fragments arranged by size:
5771 573 158
AclI AA'CG_TT
Cuts at: 1563 4629 5002 1563
Size: 3066 373 3063
Fragments arranged by size:
3066 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 2666 2699 3581 4565 5949 6217 1632
Size: 731 303 33 882 984 1384 268 1917
Fragments arranged by size:
1917 1384 984 882 731 303 268 33
AflII C'TTAA_G
Cuts at: 820 1017 3644 820
Size: 197 2627 3678
Fragments arranged by size:
3678 2627 197
AflIII A'CryG_T
Cuts at: 1203 5401 6378 1203
Size: 4198 977 1327
Fragments arranged by size:
4198 1327 977
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 5231 5231
Size: 6502
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2598 1184
Size: 1414 5088
AlwNI CAG_nnn'CTG
Cuts at: 2266 2753 3397 4245 5817 2266
Size: 487 644 848 1572 2951
Fragments arranged by size:
2951 1572 848 644 487
ApaLI G'TGCA_C
Cuts at: 4559 5715 4559
Size: 1156 5346
ApoI r'AATT_y
Cuts at: 1493 3261 3605 3696 3811 4037 4189 6298 1493
Size: 1768 344 91 115 226 152 2109 1697
Fragments arranged by size:
2109 1768 1697 344 226 152 115 91
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 2059 2420 3827 1870
Size: 73 116 361 1407 4545
Fragments arranged by size:
4545 1407 361 116 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3084 3117 3084
Size: 33 6469
BamHI G'GATC_C
Cuts at: 2391 3114 3832 4199 2391
Size: 723 718 367 4694
Fragments arranged by size:
4694 723 718 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 3092 3250 3823 5278
Size: 325 217 627 261 1044 158 573 1455
Cuts at: 5278 618
Size: 1842
Fragments arranged by size:
1842 1455 1044 627 573 325 261 217
158
BanII G_rGCy'C
Cuts at: 1391 2109 3477 3821 1391
Size: 718 1368 344 4072
Fragments arranged by size:
4072 1368 718 344
BbeI G_GCGC'C
Cuts at: 2052 2052
Size: 6502
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5531
BbvCI CC'TCA_GC
Cuts at: 2667 2667
Size: 6502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4693 4727 1903
Size: 34 2756 34 3678
Fragments arranged by size:
3678 2756 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4394 5604 4394
Size: 1210 5292
BclI T'GATC_A
Cuts at: 1548 2130 1548
Size: 582 5920
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2877 3050 5113
Size: 107 122 71 631 605 1205 173 2063
Cuts at: 5113 136
Size: 1525
Fragments arranged by size:
2063 1525 1205 631 605 173 122 107
71
BglII A'GATC_T
Cuts at: 6497 6497
Size: 6502
BlpI GC'TnA_GC
Cuts at: 3919 3919
Size: 6502
Bme1580I G_kGCm'C
Cuts at: 1790 4563 5719 1790
Size: 2773 1156 2573
Fragments arranged by size:
2773 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4233 5191 442
Size: 416 53 83 399 24 2816 958 1753
Fragments arranged by size:
2816 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6502
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3147 3147
Size: 6502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2007 3067 3491 5162 1239
Size: 768 1060 424 1671 2579
Fragments arranged by size:
2579 1671 1060 768 424
Bpu10I CC'TnA_GC
Cuts at: 2553 2667 2553
Size: 114 6388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 4627 5492 5790 2285
Size: 2342 865 298 2997
Fragments arranged by size:
2997 2342 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5165 882
Size: 617 3666 2219
Fragments arranged by size:
3666 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4293 493
Size: 711 3089 2702
Fragments arranged by size:
3089 2702 711
BsaBI GATnn'nnATC
Cuts at: 6496 6496
Size: 6502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 2055 4309 4691
Size: 53 83 186 936 516 6 2254 382
Cuts at: 4691 5326 275
Size: 635 1451
Fragments arranged by size:
2254 1451 936 635 516 382 186 83
53 6
BsaWI w'CCGG_w
Cuts at: 2388 4935 5333 5607 5754 6165 2388
Size: 2547 398 274 147 411 2725
Fragments arranged by size:
2725 2547 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3362 3392 1178
Size: 30 165 30 1959 30 4288
Fragments arranged by size:
4288 1959 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3720 1186
Size: 2534 3968
BseYI C'CCAG_C
Cuts at: 2404 5705 6201 2404
Size: 3301 496 2705
Fragments arranged by size:
3301 2705 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2811 1163
Size: 573 1075 4854
Fragments arranged by size:
4854 1075 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3952 4713 5741 1540
Size: 275 186 1951 761 1028 2301
Fragments arranged by size:
2301 1951 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2893 3477 4563 4648 5719 2893
Size: 584 1086 85 1071 3676
Fragments arranged by size:
3676 1086 1071 584 85
BsmI GAATG_Cn'
Cuts at: 4013 4106 4013
Size: 93 6409
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2893 3477 3821 4563 4648 5719
Size: 399 319 784 584 344 742 85 1071
Cuts at: 5719 1391
Size: 2174
Fragments arranged by size:
2174 1071 784 742 584 399 344 319
85
BspEI T'CCGG_A
Cuts at: 2388 6165 2388
Size: 3777 2725
BspHI T'CATG_A
Cuts at: 3733 4391 6121 3733
Size: 658 1730 4114
Fragments arranged by size:
4114 1730 658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2374 2931 3857 844
Size: 1530 557 926 3489
Fragments arranged by size:
3489 1530 926 557
BsrBI CCG'CTC
Cuts at: 1394 1823 3112 4389 1394
Size: 429 1289 1277 3507
Fragments arranged by size:
3507 1289 1277 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3018 3314 4997 5171 65
Size: 1183 1770 296 1683 174 1396
Fragments arranged by size:
1770 1683 1396 1183 296 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 5146 5333 1700
Size: 110 241 3095 187 2869
Fragments arranged by size:
3095 2869 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 2331 96
Size: 2235 4267
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 4562 5574 4562
Size: 1012 5490
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3002 3316 794
Size: 2208 314 3980
Fragments arranged by size:
3980 2208 314
BstBI TT'CG_AA
Cuts at: 2460 5306 2460
Size: 2846 3656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6502
BstXI CCAn_nnnn'nTGG
Cuts at: 2938 2938
Size: 6502
BstYI r'GATC_y
Cuts at: 1972 2391 2941 3114 3832 4199 4584 4601 6053
Size: 419 550 173 718 367 385 17 1452
Cuts at: 6053 6139 6151 6497 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 718 550 419 385 367 346
173 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3674 3674
Size: 6502
BtgI C'CryG_G
Cuts at: 513 1065 2004 2364 2398 3006 3366 513
Size: 552 939 360 34 608 360 3649
Fragments arranged by size:
3649 939 608 552 360 360 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2675 2687 487
Size: 683 56 1449 12 4302
Fragments arranged by size:
4302 1449 683 56 12
BtsI GCAGTG_nn'
Cuts at: 2906 3202 4018 4812 4832 2906
Size: 296 816 794 20 4576
Fragments arranged by size:
4576 816 794 296 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6467
DraI TTT'AAA
Cuts at: 3806 3806
Size: 6502
DraIII CAC_nnn'GTG
Cuts at: 2311 2329 2311
Size: 18 6484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2257 2869 3949
Size: 54 1425 50 275 18 427 612 1080
Cuts at: 3949 4838 6467 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1080 889 612 427 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3949 1537
Size: 275 2137 4090
Fragments arranged by size:
4090 2137 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2339 2492 2718 3144 3184 3240 4432 1353
Size: 986 153 226 426 40 56 1192 3423
Fragments arranged by size:
3423 1192 986 426 226 153 56 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5089 5463 5609 387
Size: 1064 138 3500 374 146 1280
Fragments arranged by size:
3500 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3475 3475
Size: 6502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2363 2666 2699 3067 3491 3581
Size: 393 375 356 303 33 368 424 90
Cuts at: 3581 4565 5162 5949 6217 1239
Size: 984 597 787 268 1524
Fragments arranged by size:
1524 984 787 597 424 393 375 368
356 303 268 90 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3563 3563
Size: 6502
EcoO109I rG'GnC_Cy
Cuts at: 2547 2547
Size: 6502
EcoRI G'AATT_C
Cuts at: 3696 3811 4189 3696
Size: 115 378 6009
Fragments arranged by size:
6009 378 115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3298 3298
Size: 6502
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 2052 3559 3622 5649 1678
Size: 374 1507 63 2027 2531
Fragments arranged by size:
2531 2027 1507 374 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2800 2832 2947 2950 2979
Size: 32 135 32 1228 32 115 3 29
Cuts at: 2979 2982 3013 3045 3058 3090 3211 3243 3344
Size: 3 31 32 13 32 121 32 101
Cuts at: 3344 3376 3459 3491 5143 5175 5217 5249 1373
Size: 32 83 32 1652 32 42 32 2626
Fragments arranged by size:
2626 1652 1228 135 121 115 101 83
42 32 32 32 32 32 32 32
32 32 32 31 29 13 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2218 2527 3381 3846 4092 669
Size: 988 281 280 309 854 465 246 3079
Fragments arranged by size:
3079 988 854 465 309 281 280 246
HindIII A'AGCT_T
Cuts at: 748 2693 748
Size: 1945 4557
HpaI GTT'AAC
Cuts at: 2527 4092 2527
Size: 1565 4937
KasI G'GCGC_C
Cuts at: 2048 2048
Size: 6502
KpnI G_GTAC'C
Cuts at: 3096 3254 3827 3096
Size: 158 573 5771
Fragments arranged by size:
5771 573 158
MfeI C'AATT_G
Cuts at: 4101 4101
Size: 6502
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2663 2918 5616 5800 1305
Size: 1358 255 2698 184 2007
Fragments arranged by size:
2698 2007 1358 255 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2871 10
Size: 54 1425 343 1039 3641
Fragments arranged by size:
3641 1425 1039 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3011 3738 4460 4819 4978 518
Size: 648 1845 727 722 359 159 2042
Fragments arranged by size:
2042 1845 727 722 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2400 2433 2654 3298 3323 3918 4595
Size: 440 354 33 221 644 25 595 677
Cuts at: 4595 5743 5988 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 644 595 440 354 245
221 33 25
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 6502
NarI GG'CG_CC
Cuts at: 2049 2049
Size: 6502
NcoI C'CATG_G
Cuts at: 513 1065 2004 3006 513
Size: 552 939 1002 4009
Fragments arranged by size:
4009 1002 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6502
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 6502
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6502
NotI GC'GGCC_GC
Cuts at: 3949 3949
Size: 6502
NspI r_CATG'y
Cuts at: 1132 1229 2224 3860 5405 1132
Size: 97 995 1636 1545 2229
Fragments arranged by size:
2229 1636 1545 995 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6502
PciI A'CATG_T
Cuts at: 5401 5401
Size: 6502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2882 1108
Size: 1774 4728
PfoI T'CCnGG_A
Cuts at: 3429 3429
Size: 6502
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 6084 6116 4571
Size: 32 1481 32 4957
Fragments arranged by size:
4957 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 3104 4072 3104
Size: 968 5534
PstI C_TGCA'G
Cuts at: 830 2659 2913 3061 3854 830
Size: 1829 254 148 793 3478
Fragments arranged by size:
3478 1829 793 254 148
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
PvuII CAG'CTG
Cuts at: 2654 3298 2654
Size: 644 5858
SacI G_AGCT'C
Cuts at: 3477 3477
Size: 6502
SacII CC_GC'GG
Cuts at: 2401 2401
Size: 6502
SalI G'TCGA_C
Cuts at: 1936 2216 3844 1936
Size: 280 1628 4594
Fragments arranged by size:
4594 1628 280
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 4750 6462 4750
Size: 1712 4790
SexAI A'CCwGG_T
Cuts at: 2307 2307
Size: 6502
SfcI C'TryA_G
Cuts at: 826 1045 2655 2909 3057 3478 3850 4985 5666
Size: 219 1610 254 148 421 372 1135 681
Cuts at: 5666 5857 826
Size: 191 1471
Fragments arranged by size:
1610 1471 1135 681 421 372 254 219
191 148
SfoI GGC'GCC
Cuts at: 2050 2050
Size: 6502
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 2051 1700
Size: 351 6151
SmaI CCC'GGG
Cuts at: 1872 2061 2422 3829 1872
Size: 189 361 1407 4545
Fragments arranged by size:
4545 1407 361 189
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2300 3644 4606 5507 5769 820
Size: 197 926 357 1344 962 901 262 1553
Fragments arranged by size:
1553 1344 962 926 901 357 262 197
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 3659 152
Size: 3507 2995
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 2613 1517
Size: 1096 5406
StyI C'CwwG_G
Cuts at: 513 1065 2004 3006 3282 3600 3941 513
Size: 552 939 1002 276 318 341 3074
Fragments arranged by size:
3074 1002 939 552 341 318 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2756 4529 4546 4699 4884 5329 325
Size: 1255 1176 1773 17 153 185 445 1498
Fragments arranged by size:
1773 1498 1255 1176 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2088 2331 2372
Size: 274 80 33 51 1443 111 243 41
Cuts at: 2372 4748 6460 96
Size: 2376 1712 138
Fragments arranged by size:
2376 1712 1443 274 243 138 111 80
51 41 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6462 6494 6462
Size: 32 6470
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6502
XbaI T'CTAG_A
Cuts at: 3145 3838 3145
Size: 693 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2879 2879
Size: 6502
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6502
XmaI C'CCGG_G
Cuts at: 1870 2059 2420 3827 1870
Size: 189 361 1407 4545
Fragments arranged by size:
4545 1407 361 189
XmnI GAAnn'nnTTC
Cuts at: 2456 4631 2456
Size: 2175 4327
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4310 276
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BfrBI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI
SrfI SwaI