Restriction map for
pFN21AB7814
(Circular) (Six-base) MAPSORT of: pFN21AB7814.seq Check: 6716 from: 1 to: 6466
pFN21AB7814 6466 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3634 3634
Size: 6466
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3332 4276 278
Size: 53 83 186 936 1796 944 2468
Fragments arranged by size:
2468 1796 944 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 3009 3809 1937
Size: 487 585 800 4594
Fragments arranged by size:
4594 800 585 487
Acc65I G'GTAC_C
Cuts at: 3787 3787
Size: 6466
AclI AA'CG_TT
Cuts at: 1563 4593 4966 1563
Size: 3030 373 3063
Fragments arranged by size:
3063 3030 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3137 3280 4529 5913 6181 1632
Size: 1505 143 1249 1384 268 1917
Fragments arranged by size:
1917 1505 1384 1249 268 143
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6269
AflIII A'CryG_T
Cuts at: 1203 2697 3318 5365 6342 1203
Size: 1494 621 2047 977 1327
Fragments arranged by size:
2047 1494 1327 977 621
AgeI A'CCGG_T
Cuts at: 5297 5297
Size: 6466
AhdI GACnn_n'nnGTC
Cuts at: 5195 5195
Size: 6466
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6466
AlwNI CAG_nnn'CTG
Cuts at: 2543 4209 5781 2543
Size: 1666 1572 3228
Fragments arranged by size:
3228 1666 1572
ApaI G_GGCC'C
Cuts at: 2737 2737
Size: 6466
ApaLI G'TGCA_C
Cuts at: 2705 4523 5679 2705
Size: 1818 1156 3492
Fragments arranged by size:
3492 1818 1156
ApoI r'AATT_y
Cuts at: 1493 3775 4001 4153 6262 1493
Size: 2282 226 152 2109 1697
Fragments arranged by size:
2282 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5020 160
Size: 872 3988 1606
Fragments arranged by size:
3988 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
AvaI C'yCGr_G
Cuts at: 1870 1943 2619 3791 1870
Size: 73 676 1172 4545
Fragments arranged by size:
4545 1172 676 73
BamHI G'GATC_C
Cuts at: 3796 4163 3796
Size: 367 6099
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2810 3464 3787 5242 618
Size: 325 217 627 1023 654 323 1455 1842
Fragments arranged by size:
1842 1455 1023 654 627 325 323 217
BanII G_rGCy'C
Cuts at: 1391 2737 3785 1391
Size: 1346 1048 4072
Fragments arranged by size:
4072 1346 1048
BbeI G_GCGC'C
Cuts at: 3468 3468
Size: 6466
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5495
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3552 3586 4657 4691 1903
Size: 34 1615 34 1071 34 3678
Fragments arranged by size:
3678 1615 1071 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4358 5568 4358
Size: 1210 5256
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6466
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2126 5077 136
Size: 107 122 71 631 605 454 2951 1525
Fragments arranged by size:
2951 1525 631 605 454 122 107 71
BglII A'GATC_T
Cuts at: 2377 6461 2377
Size: 4084 2382
BlpI GC'TnA_GC
Cuts at: 3883 3883
Size: 6466
Bme1580I G_kGCm'C
Cuts at: 1790 2709 2737 4527 5683 1790
Size: 919 28 1790 1156 2573
Fragments arranged by size:
2573 1790 1156 919 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6466
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2781 4197 5155
Size: 416 53 83 399 24 1364 1416 958
Cuts at: 5155 442
Size: 1753
Fragments arranged by size:
1753 1416 1364 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3311 1056
Size: 2255 4211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 5126 1239
Size: 1085 2802 2579
Fragments arranged by size:
2802 2579 1085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 4591 5456 5754 2564
Size: 2027 865 298 3276
Fragments arranged by size:
3276 2027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5129 882
Size: 617 3630 2219
Fragments arranged by size:
3630 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2677 4257 493
Size: 711 1473 1580 2702
Fragments arranged by size:
2702 1580 1473 711
BsaBI GATnn'nnATC
Cuts at: 6460 6460
Size: 6466
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2118 2943 3285 3329
Size: 53 83 186 936 585 825 342 44
Cuts at: 3329 3465 4273 4655 5290 275
Size: 136 808 382 635 1451
Fragments arranged by size:
1451 936 825 808 635 585 382 342
186 136 83 53 44
BsaWI w'CCGG_w
Cuts at: 2190 4899 5297 5571 5718 6129 2190
Size: 2709 398 274 147 411 2527
Fragments arranged by size:
2709 2527 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6241
Fragments arranged by size:
6241 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 3505 3684 3687 1186
Size: 883 1436 179 3 3965
Fragments arranged by size:
3965 1436 883 179 3
BseYI C'CCAG_C
Cuts at: 2062 3398 3411 5669 6165 2062
Size: 1336 13 2258 496 2363
Fragments arranged by size:
2363 2258 1336 496 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2506 3249 3691 1163
Size: 573 770 743 442 3938
Fragments arranged by size:
3938 770 743 573 442
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2130 3916 4677 5705 1540
Size: 275 186 129 1786 761 1028 2301
Fragments arranged by size:
2301 1786 1028 761 275 186 129
BsiHKAI G_wGCw'C
Cuts at: 2709 2995 4527 4612 5683 2709
Size: 286 1532 85 1071 3492
Fragments arranged by size:
3492 1532 1071 286 85
BsmI GAATG_Cn'
Cuts at: 2611 3159 3977 4070 2611
Size: 548 818 93 5007
Fragments arranged by size:
5007 818 548 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3320 3320
Size: 6466
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2709 2737 2995 3785 4527 4612 5683
Size: 399 919 28 258 790 742 85 1071
Cuts at: 5683 1391
Size: 2174
Fragments arranged by size:
2174 1071 919 790 742 399 258 85
28
BspEI T'CCGG_A
Cuts at: 2190 6129 2190
Size: 3939 2527
BspHI T'CATG_A
Cuts at: 4355 6085 4355
Size: 1730 4736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3634 3821 844
Size: 2790 187 3489
Fragments arranged by size:
3489 2790 187
BsrBI CCG'CTC
Cuts at: 1394 1823 2139 3191 4353 1394
Size: 429 316 1052 1162 3507
Fragments arranged by size:
3507 1162 1052 429 316
BsrDI GCAATG_nn'
Cuts at: 65 1248 4961 5135 65
Size: 1183 3713 174 1396
Fragments arranged by size:
3713 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2245 2490 5110 5297 1700
Size: 110 435 245 2620 187 2869
Fragments arranged by size:
2869 2620 435 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 2349 3213 96
Size: 2253 864 3349
Fragments arranged by size:
3349 2253 864
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6466
BssSI C'ACGA_G
Cuts at: 2089 2474 4526 5538 2089
Size: 385 2052 1012 3017
Fragments arranged by size:
3017 2052 1012 385
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2493 794
Size: 1699 4767
BstBI TT'CG_AA
Cuts at: 5270 5270
Size: 6466
BstEII G'GTnAC_C
Cuts at: 1184 3096 3574 1184
Size: 1912 478 4076
Fragments arranged by size:
4076 1912 478
BstYI r'GATC_y
Cuts at: 1972 2377 3240 3796 4163 4548 4565 6017 6103
Size: 405 863 556 367 385 17 1452 86
Cuts at: 6103 6115 6461 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 863 556 405 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2802 3468 3558 3672 513
Size: 552 1737 666 90 114 3307
Fragments arranged by size:
3307 1737 666 552 114 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3184 487
Size: 683 56 1958 3769
Fragments arranged by size:
3769 1958 683 56
BtsI GCAGTG_nn'
Cuts at: 3651 3982 4776 4796 3651
Size: 331 794 20 5321
Fragments arranged by size:
5321 794 331 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6466
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6431
DraI TTT'AAA
Cuts at: 3770 3770
Size: 6466
DraIII CAC_nnn'GTG
Cuts at: 2215 2766 2215
Size: 551 5915
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5473 809
Size: 507 4157 1802
Fragments arranged by size:
4157 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2671 2682
Size: 54 1425 50 275 18 297 544 11
Cuts at: 2682 3471 3913 4802 6431 8
Size: 789 442 889 1629 43
Fragments arranged by size:
1629 1425 889 789 544 442 297 275
54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2127 3913 1537
Size: 275 315 1786 4090
Fragments arranged by size:
4090 1786 315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 3650 4396 1353
Size: 2221 76 746 3423
Fragments arranged by size:
3423 2221 746 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3143 3209 5053 5427 5573 387
Size: 1064 138 1554 66 1844 374 146 1280
Fragments arranged by size:
1844 1554 1280 1064 374 146 138 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 3137 3280 4529 5126 5913 6181
Size: 393 692 813 143 1249 597 787 268
Cuts at: 6181 1239
Size: 1524
Fragments arranged by size:
1524 1249 813 787 692 597 393 268
143
EcoO109I rG'GnC_Cy
Cuts at: 3484 3484
Size: 6466
EcoRI G'AATT_C
Cuts at: 3775 4153 3775
Size: 378 6088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2283 3679 2283
Size: 1396 5070
FspI TGC'GCA
Cuts at: 4972 4972
Size: 6466
HaeII r_GCGC'y
Cuts at: 1678 2225 3004 3212 3468 3663 3717 5613 1678
Size: 547 779 208 256 195 54 1896 2531
Fragments arranged by size:
2531 1896 779 547 256 208 195 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3378 3410 5107 5139 5181
Size: 32 135 32 1806 32 1697 32 42
Cuts at: 5181 5213 1373
Size: 32 2626
Fragments arranged by size:
2626 1806 1697 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2662 3289 3316 3810 4056 669
Size: 988 281 724 627 27 494 246 3079
Fragments arranged by size:
3079 988 724 627 494 281 246 27
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6466
HpaI GTT'AAC
Cuts at: 4056 4056
Size: 6466
KasI G'GCGC_C
Cuts at: 3464 3464
Size: 6466
KpnI G_GTAC'C
Cuts at: 3791 3791
Size: 6466
MfeI C'AATT_G
Cuts at: 4065 4065
Size: 6466
MluI A'CGCG_T
Cuts at: 6342 6342
Size: 6466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 5580 5764 1305
Size: 903 3372 184 2007
Fragments arranged by size:
3372 2007 903 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3473 10
Size: 54 1425 343 1641 3003
Fragments arranged by size:
3003 1641 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2702 3081 3275 3443 4424 4783 4942
Size: 648 1536 379 194 168 981 359 159
Cuts at: 4942 518
Size: 2042
Fragments arranged by size:
2042 1536 981 648 379 359 194 168
159
MspA1I CmG'CkG
Cuts at: 1606 2062 2070 3882 4559 5707 5952 1606
Size: 456 8 1812 677 1148 245 2120
Fragments arranged by size:
2120 1812 1148 677 456 245 8
NarI GG'CG_CC
Cuts at: 3465 3465
Size: 6466
NcoI C'CATG_G
Cuts at: 513 1065 3468 3558 513
Size: 552 2403 90 3421
Fragments arranged by size:
3421 2403 552 90
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6466
NheI G'CTAG_C
Cuts at: 1052 3307 1052
Size: 2255 4211
NotI GC'GGCC_GC
Cuts at: 3913 3913
Size: 6466
NspI r_CATG'y
Cuts at: 1132 1229 2701 3824 5369 1132
Size: 97 1472 1123 1545 2229
Fragments arranged by size:
2229 1545 1472 1123 97
PasI CC'CwG_GG
Cuts at: 1348 3250 1348
Size: 1902 4564
PciI A'CATG_T
Cuts at: 2697 5365 2697
Size: 2668 3798
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2680 1108
Size: 1572 4894
PmeI GTTT'AAAC
Cuts at: 3770 3770
Size: 6466
PmlI CAC'GTG
Cuts at: 2677 2677
Size: 6466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4535 4567 6048 6080 4535
Size: 32 1481 32 4921
Fragments arranged by size:
4921 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3484 3484
Size: 6466
PshAI GACnn'nnGTC
Cuts at: 1613 2657 1613
Size: 1044 5422
PsiI TTA'TAA
Cuts at: 4036 4036
Size: 6466
PspOMI G'GGCC_C
Cuts at: 2733 2733
Size: 6466
PstI C_TGCA'G
Cuts at: 830 3818 830
Size: 2988 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6466
SalI G'TCGA_C
Cuts at: 1936 3808 1936
Size: 1872 4594
SanDI GG'GwC_CC
Cuts at: 3484 3484
Size: 6466
SapI GCTCTTCn'nnn_
Cuts at: 3650 3650
Size: 6466
SbfI CC_TGCA'GG
Cuts at: 3818 3818
Size: 6466
ScaI AGT'ACT
Cuts at: 3406 4714 6426 3406
Size: 1308 1712 3446
Fragments arranged by size:
3446 1712 1308
SexAI A'CCwGG_T
Cuts at: 3132 3132
Size: 6466
SfcI C'TryA_G
Cuts at: 826 1045 3355 3592 3814 4949 5630 5821 826
Size: 219 2310 237 222 1135 681 191 1471
Fragments arranged by size:
2310 1471 1135 681 237 222 219 191
SfoI GGC'GCC
Cuts at: 3466 3466
Size: 6466
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6466
SmaI CCC'GGG
Cuts at: 1872 3793 1872
Size: 1921 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 2619 4570 5471 5733 820
Size: 197 926 636 40 1951 901 262 1553
Fragments arranged by size:
1951 1553 926 901 636 262 197 40
SnaBI TAC'GTA
Cuts at: 493 4257 493
Size: 3764 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6466
SphI G_CATG'C
Cuts at: 1132 3824 1132
Size: 2692 3774
SspI AAT'ATT
Cuts at: 5 52 2026 4390 5
Size: 47 1974 2364 2081
Fragments arranged by size:
2364 2081 1974 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6466
StyI C'CwwG_G
Cuts at: 513 1065 3102 3468 3558 3905 513
Size: 552 2037 366 90 347 3074
Fragments arranged by size:
3074 2037 552 366 347 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3562 4493 4510 4663 4848 5293 325
Size: 1255 1982 931 17 153 185 445 1498
Fragments arranged by size:
1982 1498 1255 931 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 3213 3404
Size: 274 80 33 51 1443 372 864 191
Cuts at: 3404 4712 6424 96
Size: 1308 1712 138
Fragments arranged by size:
1712 1443 1308 864 372 274 191 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2699 2731 6426 6458 2699
Size: 32 3695 32 2707
Fragments arranged by size:
3695 2707 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3347 1316
Size: 2031 4435
XbaI T'CTAG_A
Cuts at: 3802 3802
Size: 6466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3556 3556
Size: 6466
XhoI C'TCGA_G
Cuts at: 1943 2619 1943
Size: 676 5790
XmaI C'CCGG_G
Cuts at: 1870 3791 1870
Size: 1921 4545
XmnI GAAnn'nnTTC
Cuts at: 4595 4595
Size: 6466
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3330 4274 276
Size: 53 83 186 936 1796 944 2468
Fragments arranged by size:
2468 1796 944 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PspXI PsrI PvuII RsrII SacI SacII SfiI SrfI
SwaI