Restriction map for
pFN21AB7812
(Circular) (Six-base) MAPSORT of: pFN21AB7812.seq Check: 4817 from: 1 to: 6490
pFN21AB7812 6490 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2326 3833 1937
Size: 389 1507 4594
Fragments arranged by size:
4594 1507 389
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 3456 4553 5937 6205 1632
Size: 437 1387 1097 1384 268 1917
Fragments arranged by size:
1917 1387 1384 1097 437 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 3620 5389 6366 1203
Size: 2417 1769 977 1327
Fragments arranged by size:
2417 1769 1327 977
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 5219 5219
Size: 6490
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6490
AlwNI CAG_nnn'CTG
Cuts at: 4233 5805 4233
Size: 1572 4918
ApaLI G'TGCA_C
Cuts at: 4547 5703 4547
Size: 1156 5334
ApoI r'AATT_y
Cuts at: 1493 2039 2083 2335 2474 2482 2586 2602 2670
Size: 546 44 252 139 8 104 16 68
Cuts at: 2670 2754 2922 3342 3594 3678 3799 4025 4177
Size: 84 168 420 252 84 121 226 152
Cuts at: 4177 6286 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 546 420 252 252 226 168
152 139 121 104 84 84 68 44
16 8
AseI AT'TA_AT
Cuts at: 160 1032 2345 5044 160
Size: 872 1313 2699 1606
Fragments arranged by size:
2699 1606 1313 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 2253 3815 1870
Size: 73 310 1562 4545
Fragments arranged by size:
4545 1562 310 73
BamHI G'GATC_C
Cuts at: 3820 4187 3820
Size: 367 6123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3811 5266 618
Size: 325 217 627 2024 1455 1842
Fragments arranged by size:
2024 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3809 1391
Size: 2418 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 928
Size: 971 220 5299
Fragments arranged by size:
5299 971 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4681 4715 1903
Size: 34 2744 34 3678
Fragments arranged by size:
3678 2744 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4382 5592 4382
Size: 1210 5280
BclI T'GATC_A
Cuts at: 1548 2160 1548
Size: 612 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5101 136
Size: 107 122 71 631 605 3429 1525
Fragments arranged by size:
3429 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6485 6485
Size: 6490
BlpI GC'TnA_GC
Cuts at: 3907 3907
Size: 6490
Bme1580I G_kGCm'C
Cuts at: 1790 4551 5707 1790
Size: 2761 1156 2573
Fragments arranged by size:
2761 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4221 5179 442
Size: 416 53 83 399 24 2804 958 1753
Fragments arranged by size:
2804 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2212 2784 2868 2952 3036 3120 3204
Size: 953 20 572 84 84 84 84 84
Cuts at: 3204 3288 3372 3540 3624 3708 5150 1239
Size: 84 84 168 84 84 1442 2579
Fragments arranged by size:
2579 1442 953 572 168 84 84 84
84 84 84 84 84 84 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4615 5480 5778 4615
Size: 865 298 5327
Fragments arranged by size:
5327 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2250 5153 882
Size: 617 751 2903 2219
Fragments arranged by size:
2903 2219 751 617
BsaAI yAC'GTr
Cuts at: 493 1204 4281 493
Size: 711 3077 2702
Fragments arranged by size:
3077 2702 711
BsaBI GATnn'nnATC
Cuts at: 6484 6484
Size: 6490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4297 4679 5314 275
Size: 53 83 186 936 2764 382 635 1451
Fragments arranged by size:
2764 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3770 4923 5321 5595 5742 6153 3770
Size: 1153 398 274 147 411 4107
Fragments arranged by size:
4107 1153 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6265
Fragments arranged by size:
6265 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6490
BseYI C'CCAG_C
Cuts at: 5693 6189 5693
Size: 496 5994
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2058 3051 1163
Size: 573 322 993 4602
Fragments arranged by size:
4602 993 573 322
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3940 4701 5729 1540
Size: 275 186 1939 761 1028 2301
Fragments arranged by size:
2301 1939 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4551 4636 5707 4551
Size: 85 1071 5334
Fragments arranged by size:
5334 1071 85
BsmI GAATG_Cn'
Cuts at: 4001 4094 4001
Size: 93 6397
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3809 4551 4636 5707 1391
Size: 399 2019 742 85 1071 2174
Fragments arranged by size:
2174 2019 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6153 6153
Size: 6490
BspHI T'CATG_A
Cuts at: 4379 6109 4379
Size: 1730 4760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3845 844
Size: 3001 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4377 1394
Size: 429 2554 3507
Fragments arranged by size:
3507 2554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2066 4985 5159 65
Size: 1183 818 2919 174 1396
Fragments arranged by size:
2919 1396 1183 818 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5134 5321 1700
Size: 110 3324 187 2869
Fragments arranged by size:
3324 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6490
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 4550 5562 4550
Size: 1012 5478
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6490
BstBI TT'CG_AA
Cuts at: 5294 5294
Size: 6490
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 3820 4187 4572 4589 6041 6127 6139 6485
Size: 1848 367 385 17 1452 86 12 346
Cuts at: 6485 1972
Size: 1977
Fragments arranged by size:
1977 1848 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4999
Fragments arranged by size:
4999 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5751
Fragments arranged by size:
5751 683 56
BtsI GCAGTG_nn'
Cuts at: 2070 4006 4800 4820 2070
Size: 1936 794 20 3740
Fragments arranged by size:
3740 1936 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6455
DraI TTT'AAA
Cuts at: 3226 3478 3730 3794 3226
Size: 252 252 64 5922
Fragments arranged by size:
5922 252 252 64
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5497 809
Size: 507 4181 1802
Fragments arranged by size:
4181 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2220 2264
Size: 54 1425 50 275 18 201 189 44
Cuts at: 2264 3937 4826 6455 8
Size: 1673 889 1629 43
Fragments arranged by size:
1673 1629 1425 889 275 201 189 54
50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3937 1537
Size: 275 2125 4090
Fragments arranged by size:
4090 2125 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 3238 3432 4420 1353
Size: 842 1043 194 988 3423
Fragments arranged by size:
3423 1043 988 842 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5077 5451 5597 387
Size: 1064 138 3488 374 146 1280
Fragments arranged by size:
3488 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2192 2212 2784 2868 2952 3036
Size: 393 437 123 20 572 84 84 84
Cuts at: 3036 3120 3204 3288 3372 3456 3540 3624 3708
Size: 84 84 84 84 84 84 84 84
Cuts at: 3708 4553 5150 5937 6205 1239
Size: 845 597 787 268 1524
Fragments arranged by size:
1524 845 787 597 572 437 393 268
123 84 84 84 84 84 84 84
84 84 84 84 20
EcoRI G'AATT_C
Cuts at: 2039 2586 2670 3678 3799 4177 2039
Size: 547 84 1008 121 378 4352
Fragments arranged by size:
4352 1008 547 378 121 84
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 5637 1678
Size: 3959 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2152 2184 2196 2228 5131
Size: 32 135 32 580 32 12 32 2903
Cuts at: 5131 5163 5205 5237 1373
Size: 32 42 32 2626
Fragments arranged by size:
2903 2626 580 135 42 32 32 32
32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3834 4080 669
Size: 988 281 1896 246 3079
Fragments arranged by size:
3079 1896 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2883 3219 3303 3387 3555 3723 748
Size: 2135 336 84 84 168 168 3515
Fragments arranged by size:
3515 2135 336 168 168 84 84
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 4089 4089
Size: 6490
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5604 5788 1305
Size: 4299 184 2007
Fragments arranged by size:
4299 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2033 2222 10
Size: 54 1425 343 201 189 4278
Fragments arranged by size:
4278 1425 343 201 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2234 3535 4448 4807 4966 518
Size: 648 1068 1301 913 359 159 2042
Fragments arranged by size:
2042 1301 1068 913 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 3906 4583 5731 5976 1606
Size: 474 1826 677 1148 245 2120
Fragments arranged by size:
2120 1826 1148 677 474 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4999
Fragments arranged by size:
4999 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6490
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 3937 3937
Size: 6490
NspI r_CATG'y
Cuts at: 1132 1229 3624 3848 5393 1132
Size: 97 2395 224 1545 2229
Fragments arranged by size:
2395 2229 1545 224 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6490
PciI A'CATG_T
Cuts at: 3620 5389 3620
Size: 1769 4721
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6490
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 6072 6104 4559
Size: 32 1481 32 4945
Fragments arranged by size:
4945 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6490
PsiI TTA'TAA
Cuts at: 4060 4060
Size: 6490
PstI C_TGCA'G
Cuts at: 830 3842 830
Size: 3012 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SbfI CC_TGCA'GG
Cuts at: 3842 3842
Size: 6490
ScaI AGT'ACT
Cuts at: 4738 6450 4738
Size: 1712 4778
SexAI A'CCwGG_T
Cuts at: 2121 2121
Size: 6490
SfcI C'TryA_G
Cuts at: 826 1045 2113 3838 4973 5654 5845 826
Size: 219 1068 1725 1135 681 191 1471
Fragments arranged by size:
1725 1471 1135 1068 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 3817 1872
Size: 1945 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4594 5495 5757 820
Size: 197 926 2651 901 262 1553
Fragments arranged by size:
2651 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6490
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 2439 4414 5
Size: 47 2387 1975 2081
Fragments arranged by size:
2387 2081 1975 47
StuI AGG'CCT
Cuts at: 1517 2189 1517
Size: 672 5818
StyI C'CwwG_G
Cuts at: 513 1065 2004 3929 513
Size: 552 939 1925 3074
Fragments arranged by size:
3074 1925 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4517 4534 4687 4872 5317 325
Size: 1255 2937 17 153 185 445 1498
Fragments arranged by size:
2937 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4736 6448 96
Size: 274 80 33 51 1443 2759 1712 138
Fragments arranged by size:
2759 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6450 6482 6450
Size: 32 6458
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6490
XmaI C'CCGG_G
Cuts at: 1870 3815 1870
Size: 1945 4545
XmnI GAAnn'nnTTC
Cuts at: 2522 4619 2522
Size: 2097 4393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI