Restriction map for
pFN21AB7811
(Circular) (Six-base) MAPSORT of: pFN21AB7811.seq Check: 5301 from: 1 to: 6292
pFN21AB7811 6292 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3635 1937
Size: 1698 4594
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 3208 4355 5739 6007 1632
Size: 767 809 1147 1384 268 1917
Fragments arranged by size:
1917 1384 1147 809 767 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 2139 2226 5191 6168 1203
Size: 936 87 2965 977 1327
Fragments arranged by size:
2965 1327 977 936 87
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2556 3482 1184
Size: 1372 926 3994
Fragments arranged by size:
3994 1372 926
AlwNI CAG_nnn'CTG
Cuts at: 2581 3421 4035 5607 2581
Size: 840 614 1572 3266
Fragments arranged by size:
3266 1572 840 614
ApaI G_GGCC'C
Cuts at: 2024 2040 2720 2024
Size: 16 680 5596
Fragments arranged by size:
5596 680 16
ApaLI G'TGCA_C
Cuts at: 3401 4349 5505 3401
Size: 948 1156 4188
Fragments arranged by size:
4188 1156 948
ApoI r'AATT_y
Cuts at: 1493 3601 3827 3979 6088 1493
Size: 2108 226 152 2109 1697
Fragments arranged by size:
2109 2108 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 2119 2713 2928 2948 3359 3617 1870
Size: 73 176 594 215 20 411 258 4545
Fragments arranged by size:
4545 594 411 258 215 176 73 20
BamHI G'GATC_C
Cuts at: 2607 3622 3989 2607
Size: 1015 367 4910
Fragments arranged by size:
4910 1015 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2952 3613 5068 618
Size: 325 217 627 1165 661 1455 1842
Fragments arranged by size:
1842 1455 1165 661 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2040 2120 2720 2907 2927 3156 3330
Size: 633 16 80 600 187 20 229 174
Cuts at: 3330 3348 3497 3611 1391
Size: 18 149 114 4072
Fragments arranged by size:
4072 633 600 229 187 174 149 114
80 20 18 16
BbeI G_GCGC'C
Cuts at: 2956 2956
Size: 6292
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2394 928
Size: 971 495 4826
Fragments arranged by size:
4826 971 495
BbvCI CC'TCA_GC
Cuts at: 3125 3125
Size: 6292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4184 5394 3329
Size: 855 1210 4227
Fragments arranged by size:
4227 1210 855
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3352 4903 136
Size: 107 122 71 631 605 1680 1551 1525
Fragments arranged by size:
1680 1551 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 2656 3209 3709 2656
Size: 553 500 5239
Fragments arranged by size:
5239 553 500
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2040 2720 3244 3279 3405 4353 5509
Size: 234 16 680 524 35 126 948 1156
Cuts at: 5509 1790
Size: 2573
Fragments arranged by size:
2573 1156 948 680 524 234 126 35
16
BmgBI CAC'GTC
Cuts at: 1318 2434 2704 1318
Size: 1116 270 4906
Fragments arranged by size:
4906 1116 270
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4023 4981 442
Size: 416 53 83 399 24 2606 958 1753
Fragments arranged by size:
2606 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2134 2717 2737 4952 1239
Size: 895 583 20 2215 2579
Fragments arranged by size:
2579 2215 895 583 20
Bpu10I CC'TnA_GC
Cuts at: 3125 3125
Size: 6292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 4417 5282 5580 2360
Size: 2057 865 298 3072
Fragments arranged by size:
3072 2057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4955 882
Size: 617 3456 2219
Fragments arranged by size:
3456 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2140 4083 493
Size: 711 936 1943 2702
Fragments arranged by size:
2702 1943 936 711
BsaBI GATnn'nnATC
Cuts at: 6286 6286
Size: 6292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2953 3054 4099 4481
Size: 53 83 186 936 1420 101 1045 382
Cuts at: 4481 5116 275
Size: 635 1451
Fragments arranged by size:
1451 1420 1045 936 635 382 186 101
83 53
BsaWI w'CCGG_w
Cuts at: 2238 2604 4725 5123 5397 5544 5955 2238
Size: 366 2121 398 274 147 411 2575
Fragments arranged by size:
2575 2121 411 398 366 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2735 2765 3425 3455 1178
Size: 30 165 30 1332 30 660 30 4015
Fragments arranged by size:
4015 1332 660 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2915 2951 1186
Size: 1729 36 4527
Fragments arranged by size:
4527 1729 36
BseYI C'CCAG_C
Cuts at: 2039 2544 2737 2909 3484 5495 5991 2039
Size: 505 193 172 575 2011 496 2340
Fragments arranged by size:
2340 2011 575 505 496 193 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2456 2615 1163
Size: 573 720 159 4840
Fragments arranged by size:
4840 720 573 159
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2988 3742 4503 5531 1540
Size: 275 186 987 754 761 1028 2301
Fragments arranged by size:
2301 1028 987 761 754 275 186
BsiHKAI G_wGCw'C
Cuts at: 2703 3156 3330 3405 4353 4438 5509 2703
Size: 453 174 75 948 85 1071 3486
Fragments arranged by size:
3486 1071 948 453 174 85 75
BsmI GAATG_Cn'
Cuts at: 3803 3896 3803
Size: 93 6199
BsmBI CGTCTCn'nnnn_
Cuts at: 2280 2280
Size: 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2040 2120 2703 2720 2907 2927
Size: 399 234 16 80 583 17 187 20
Cuts at: 2927 3156 3244 3279 3330 3348 3405 3497 3611
Size: 229 88 35 51 18 57 92 114
Cuts at: 3611 4353 4438 5509 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 583 399 234 229 187
114 92 88 85 80 57 51 35
20 18 17 16
BspEI T'CCGG_A
Cuts at: 2238 5955 2238
Size: 3717 2575
BspHI T'CATG_A
Cuts at: 4181 5911 4181
Size: 1730 4562
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3031 3204 3474 3647 844
Size: 2187 173 270 173 3489
Fragments arranged by size:
3489 2187 270 173 173
BsrBI CCG'CTC
Cuts at: 1394 1823 2124 4179 1394
Size: 429 301 2055 3507
Fragments arranged by size:
3507 2055 429 301
BsrDI GCAATG_nn'
Cuts at: 65 1248 4787 4961 65
Size: 1183 3539 174 1396
Fragments arranged by size:
3539 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2682 3081 3469 4936 5123 1700
Size: 110 872 399 388 1467 187 2869
Fragments arranged by size:
2869 1467 872 399 388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 2078 4352 5364 2078
Size: 2274 1012 3006
Fragments arranged by size:
3006 2274 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3205 3400 794
Size: 2411 195 3686
Fragments arranged by size:
3686 2411 195
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 3268 1184
Size: 2084 4208
BstXI CCAn_nnnn'nTGG
Cuts at: 2479 2833 2479
Size: 354 5938
BstYI r'GATC_y
Cuts at: 1972 2008 2397 2607 2628 3622 3989 4374 4391
Size: 36 389 210 21 994 367 385 17
Cuts at: 4391 5843 5929 5941 6287 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 994 389 385 367 346 210
86 36 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2707 2832 2964 3217 3444
Size: 552 939 12 691 125 132 253 227
Cuts at: 3444 513
Size: 3361
Fragments arranged by size:
3361 939 691 552 253 227 132 125
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5553
Fragments arranged by size:
5553 683 56
BtsI GCAGTG_nn'
Cuts at: 3808 4602 4622 3808
Size: 794 20 5478
Fragments arranged by size:
5478 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2368 3584 5299 809
Size: 507 1052 1216 1715 1802
Fragments arranged by size:
1802 1715 1216 1052 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 2323 2481
Size: 54 1425 50 275 18 372 121 158
Cuts at: 2481 2520 2778 3312 3739 4628 6257 8
Size: 39 258 534 427 889 1629 43
Fragments arranged by size:
1629 1425 889 534 427 372 275 258
158 121 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2375 2615 4222 1353
Size: 1022 240 1607 3423
Fragments arranged by size:
3423 1607 1022 240
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2390 4879 5253 5399 387
Size: 1064 138 801 2489 374 146 1280
Fragments arranged by size:
2489 1280 1064 801 374 146 138
EcoICRI GAG'CTC
Cuts at: 3154 3328 3154
Size: 174 6118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2399 2717 2737 3208 4355 4952
Size: 393 502 265 318 20 471 1147 597
Cuts at: 4952 5739 6007 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1147 787 597 502 471 393 318
268 265 20
EcoO109I rG'GnC_Cy
Cuts at: 2021 2036 2717 2865 3529 3577 2021
Size: 15 681 148 664 48 4736
Fragments arranged by size:
4736 681 664 148 48 15
EcoRI G'AATT_C
Cuts at: 3601 3979 3601
Size: 378 5914
FseI GG_CCGG'CC
Cuts at: 3085 3085
Size: 6292
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 2956 5439 1678
Size: 1278 2483 2531
Fragments arranged by size:
2531 2483 1278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2639 2671 2781 2813 3317
Size: 32 135 32 1067 32 110 32 504
Cuts at: 3317 3349 3425 3457 4933 4965 5007 5039 1373
Size: 32 76 32 1476 32 42 32 2626
Fragments arranged by size:
2626 1476 1067 504 135 110 76 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2563 3636 3882 669
Size: 988 281 625 1073 246 3079
Fragments arranged by size:
3079 1073 988 625 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6292
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KasI G'GCGC_C
Cuts at: 2952 2952
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2826 3207 5406 5590 1305
Size: 1521 381 2199 184 2007
Fragments arranged by size:
2199 2007 1521 381 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2204 2325 2483 2522 2780
Size: 54 1425 343 372 121 158 39 258
Cuts at: 2780 3314 10
Size: 534 2988
Fragments arranged by size:
2988 1425 534 372 343 258 158 121
54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2496 3168 4250 4609 4768 518
Size: 648 1330 672 1082 359 159 2042
Fragments arranged by size:
2042 1330 1082 672 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2440 3397 3421 3708 4385 5533 5778 1606
Size: 834 957 24 287 677 1148 245 2120
Fragments arranged by size:
2120 1148 957 834 677 287 245 24
NaeI GCC'GGC
Cuts at: 2684 3083 2684
Size: 399 5893
NarI GG'CG_CC
Cuts at: 2953 2953
Size: 6292
NcoI C'CATG_G
Cuts at: 513 1065 2004 2832 513
Size: 552 939 828 3973
Fragments arranged by size:
3973 939 828 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NgoMIV G'CCGG_C
Cuts at: 2682 3081 2682
Size: 399 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 2230 2543 3650 5195 1132
Size: 97 1001 313 1107 1545 2229
Fragments arranged by size:
2229 1545 1107 1001 313 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6292
PciI A'CATG_T
Cuts at: 2226 5191 2226
Size: 2965 3327
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2070 1108
Size: 962 5330
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PmlI CAC'GTG
Cuts at: 2140 2140
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3529 3577 3529
Size: 48 6244
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PspOMI G'GGCC_C
Cuts at: 2020 2036 2716 2020
Size: 16 680 5596
Fragments arranged by size:
5596 680 16
PstI C_TGCA'G
Cuts at: 830 3201 3644 830
Size: 2371 443 3478
Fragments arranged by size:
3478 2371 443
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 2440 3397 2440
Size: 957 5335
SacI G_AGCT'C
Cuts at: 3156 3330 3156
Size: 174 6118
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 2183 4540 6252 2183
Size: 2357 1712 2223
Fragments arranged by size:
2357 2223 1712
SfcI C'TryA_G
Cuts at: 826 1045 2743 3197 3640 4775 5456 5647 826
Size: 219 1698 454 443 1135 681 191 1471
Fragments arranged by size:
1698 1471 1135 681 454 443 219 191
SfoI GGC'GCC
Cuts at: 2954 2954
Size: 6292
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 2930 2950 3619 1872
Size: 1058 20 669 4545
Fragments arranged by size:
4545 1058 669 20
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 4396 5297 5559 820
Size: 197 926 396 2057 901 262 1553
Fragments arranged by size:
2057 1553 926 901 396 262 197
SnaBI TAC'GTA
Cuts at: 493 4083 493
Size: 3590 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 2543 3650 1132
Size: 1411 1107 3774
Fragments arranged by size:
3774 1411 1107
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 3355 1517
Size: 1838 4454
StyI C'CwwG_G
Cuts at: 513 1065 2004 2163 2832 3573 3731 513
Size: 552 939 159 669 741 158 3074
Fragments arranged by size:
3074 939 741 669 552 159 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2322 4319 4336 4489 4674 5119 325
Size: 1255 742 1997 17 153 185 445 1498
Fragments arranged by size:
1997 1498 1255 742 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 4538 6250
Size: 274 80 33 51 1443 204 2357 1712
Cuts at: 6250 96
Size: 138
Fragments arranged by size:
2357 1712 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6252 6284 6252
Size: 32 6260
Tth111I GACn'n_nGTC
Cuts at: 1316 2411 1316
Size: 1095 5197
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2881 3244 2881
Size: 363 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 2928 2948 3617 1870
Size: 1058 20 669 4545
Fragments arranged by size:
4545 1058 669 20
XmnI GAAnn'nnTTC
Cuts at: 2132 4421 2132
Size: 2289 4003
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI
NruI NsiI PacI PfoI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI