Restriction map for
pFN21AB7808
(Circular) (Six-base) MAPSORT of: pFN21AB7808.seq Check: 4666 from: 1 to: 6490
pFN21AB7808 6490 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2821 2905 3833 1937
Size: 884 84 928 4594
Fragments arranged by size:
4594 928 884 84
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 3475 4617 4990 1563
Size: 1912 1142 373 3063
Fragments arranged by size:
3063 1912 1142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2176 2328 2446 3202 3286 3474 3558 3638
Size: 544 152 118 756 84 188 84 80
Cuts at: 3638 3642 4553 5937 6205 1632
Size: 4 911 1384 268 1917
Fragments arranged by size:
1917 1384 911 756 544 268 188 152
118 84 84 80 4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 2885 2992 5389 6366 1203
Size: 1682 107 2397 977 1327
Fragments arranged by size:
2397 1682 1327 977 107
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 5219 5219
Size: 6490
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6490
AlwNI CAG_nnn'CTG
Cuts at: 4233 5805 4233
Size: 1572 4918
ApaLI G'TGCA_C
Cuts at: 4547 5703 4547
Size: 1156 5334
ApoI r'AATT_y
Cuts at: 1493 2664 3061 3084 3764 3799 4025 4177 6286
Size: 1171 397 23 680 35 226 152 2109
Cuts at: 6286 1493
Size: 1697
Fragments arranged by size:
2109 1697 1171 680 397 226 152 35
23
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 3815 1870
Size: 73 1872 4545
Fragments arranged by size:
4545 1872 73
AvrII C'CTAG_G
Cuts at: 2350 2350
Size: 6490
BamHI G'GATC_C
Cuts at: 3820 4187 3820
Size: 367 6123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3811 5266 618
Size: 325 217 627 2024 1455 1842
Fragments arranged by size:
2024 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3809 1391
Size: 2418 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2575 928
Size: 971 676 4843
Fragments arranged by size:
4843 971 676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4681 4715 1903
Size: 34 2744 34 3678
Fragments arranged by size:
3678 2744 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4382 5592 4382
Size: 1210 5280
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6490
BfrBI ATG'CAT
Cuts at: 3600 3718 3600
Size: 118 6372
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5101 136
Size: 107 122 71 631 605 3429 1525
Fragments arranged by size:
3429 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3221 3661 6485 3221
Size: 440 2824 3226
Fragments arranged by size:
3226 2824 440
BlpI GC'TnA_GC
Cuts at: 3907 3907
Size: 6490
Bme1580I G_kGCm'C
Cuts at: 1790 4551 5707 1790
Size: 2761 1156 2573
Fragments arranged by size:
2761 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2006 2255 4221
Size: 416 53 83 399 24 589 249 1966
Cuts at: 4221 5179 442
Size: 958 1753
Fragments arranged by size:
1966 1753 958 589 416 399 249 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3473
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2526 2694 2778 3030 3198 3282 3384 3468
Size: 1287 168 84 252 168 84 102 84
Cuts at: 3468 3554 5150 1239
Size: 86 1596 2579
Fragments arranged by size:
2579 1596 1287 252 168 168 102 86
84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4615 5480 5778 4615
Size: 865 298 5327
Fragments arranged by size:
5327 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5153 882
Size: 617 3654 2219
Fragments arranged by size:
3654 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2886 2995 3685 4281 493
Size: 711 1682 109 690 596 2702
Fragments arranged by size:
2702 1682 711 690 596 109
BsaBI GATnn'nnATC
Cuts at: 6484 6484
Size: 6490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4297 4679 5314 275
Size: 53 83 186 936 2764 382 635 1451
Fragments arranged by size:
2764 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4923 5321 5595 5742 6153 4923
Size: 398 274 147 411 5260
Fragments arranged by size:
5260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2459 2489 1178
Size: 30 165 30 1056 30 5179
Fragments arranged by size:
5179 1056 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 5610
BseYI C'CCAG_C
Cuts at: 5693 6189 5693
Size: 496 5994
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5917
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3940 4701 5729 1540
Size: 275 186 1939 761 1028 2301
Fragments arranged by size:
2301 1939 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 4551 4636 5707 2172
Size: 2379 85 1071 2955
Fragments arranged by size:
2955 2379 1071 85
BsmI GAATG_Cn'
Cuts at: 2879 3470 3602 4001 4094 2879
Size: 591 132 399 93 5275
Fragments arranged by size:
5275 591 399 132 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 3809 4551 4636 5707 1391
Size: 399 382 1637 742 85 1071 2174
Fragments arranged by size:
2174 1637 1071 742 399 382 85
BspEI T'CCGG_A
Cuts at: 6153 6153
Size: 6490
BspHI T'CATG_A
Cuts at: 3160 3328 3516 3768 4379 6109 3160
Size: 168 188 252 611 1730 3541
Fragments arranged by size:
3541 1730 611 252 188 168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3845 844
Size: 3001 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4377 1394
Size: 429 2554 3507
Fragments arranged by size:
3507 2554 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3209 3565 3649 4985 5159 65
Size: 1183 1961 356 84 1336 174 1396
Fragments arranged by size:
1961 1396 1336 1183 356 174 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 5134 5321 1700
Size: 110 3324 187 2869
Fragments arranged by size:
3324 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6490
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 4550 5562 4550
Size: 1012 5478
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6490
BstBI TT'CG_AA
Cuts at: 2566 3762 5294 2566
Size: 1196 1532 3762
Fragments arranged by size:
3762 1532 1196
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 2810 3221 3661 3745 3820 4187 4572 4589
Size: 838 411 440 84 75 367 385 17
Cuts at: 4589 6041 6127 6139 6485 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 838 440 411 385 367 346
86 84 75 17 12
BstZ17I GTA'TAC
Cuts at: 2906 2906
Size: 6490
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4999
Fragments arranged by size:
4999 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5751
Fragments arranged by size:
5751 683 56
BtsI GCAGTG_nn'
Cuts at: 2957 3125 4006 4800 4820 2957
Size: 168 881 794 20 4627
Fragments arranged by size:
4627 881 794 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6455
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5497 809
Size: 507 4181 1802
Fragments arranged by size:
4181 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3937 4826 6455
Size: 54 1425 50 275 18 2107 889 1629
Cuts at: 6455 8
Size: 43
Fragments arranged by size:
2107 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3937 1537
Size: 275 2125 4090
Fragments arranged by size:
4090 2125 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3614 4420 1353
Size: 2261 806 3423
Fragments arranged by size:
3423 2261 806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5077 5451 5597 387
Size: 1064 138 3488 374 146 1280
Fragments arranged by size:
3488 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2328 2446 2526 2694 2778 3030
Size: 393 544 152 118 80 168 84 252
Cuts at: 3030 3198 3202 3282 3286 3384 3468 3474 3554
Size: 168 4 80 4 98 84 6 80
Cuts at: 3554 3558 3638 3642 4553 5150 5937 6205 1239
Size: 4 80 4 911 597 787 268 1524
Fragments arranged by size:
1524 911 787 597 544 393 268 252
168 168 152 118 98 84 84 80
80 80 80 6 4 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2916 2916
Size: 6490
EcoRI G'AATT_C
Cuts at: 2664 3061 3764 3799 4177 2664
Size: 397 703 35 378 4977
Fragments arranged by size:
4977 703 397 378 35
EcoRV GAT'ATC
Cuts at: 3158 3514 3158
Size: 356 6134
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 5637 1678
Size: 3959 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5131 5163 5205 5237 1373
Size: 32 135 32 3559 32 42 32 2626
Fragments arranged by size:
3559 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2444 3834 4080 669
Size: 988 281 506 1390 246 3079
Fragments arranged by size:
3079 1390 988 506 281 246
HindIII A'AGCT_T
Cuts at: 748 2298 2326 748
Size: 1550 28 4912
Fragments arranged by size:
4912 1550 28
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 4089 4089
Size: 6490
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5604 5788 1305
Size: 4299 184 2007
Fragments arranged by size:
4299 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4668
Fragments arranged by size:
4668 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2437 2559 2579 2689 2884 4448 4807
Size: 648 1271 122 20 110 195 1564 359
Cuts at: 4807 4966 518
Size: 159 2042
Fragments arranged by size:
2042 1564 1271 648 359 195 159 122
110 20
MspA1I CmG'CkG
Cuts at: 1606 3906 4583 5731 5976 1606
Size: 2300 677 1148 245 2120
Fragments arranged by size:
2300 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4999
Fragments arranged by size:
4999 939 552
NdeI CA'TA_TG
Cuts at: 387 2404 387
Size: 2017 4473
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 3937 3937
Size: 6490
NsiI A_TGCA'T
Cuts at: 3602 3720 3602
Size: 118 6372
NspI r_CATG'y
Cuts at: 1132 1229 3722 3848 5393 1132
Size: 97 2493 126 1545 2229
Fragments arranged by size:
2493 2229 1545 126 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6490
PciI A'CATG_T
Cuts at: 5389 5389
Size: 6490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6490
PfoI T'CCnGG_A
Cuts at: 2286 2286
Size: 6490
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PmlI CAC'GTG
Cuts at: 2886 2886
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2143 4559 4591 6072 6104 2111
Size: 32 2416 32 1481 32 2497
Fragments arranged by size:
2497 2416 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2916 2916
Size: 6490
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6490
PsiI TTA'TAA
Cuts at: 2604 3378 4060 2604
Size: 774 682 5034
Fragments arranged by size:
5034 774 682
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3486 3518 3486
Size: 32 6458
PstI C_TGCA'G
Cuts at: 830 3842 830
Size: 3012 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SbfI CC_TGCA'GG
Cuts at: 3842 3842
Size: 6490
ScaI AGT'ACT
Cuts at: 4738 6450 4738
Size: 1712 4778
SfcI C'TryA_G
Cuts at: 826 1045 2089 2130 3838 4973 5654 5845 826
Size: 219 1044 41 1708 1135 681 191 1471
Fragments arranged by size:
1708 1471 1135 1044 681 219 191 41
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 3817 1872
Size: 1945 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4594 5495 5757 820
Size: 197 926 2651 901 262 1553
Fragments arranged by size:
2651 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6490
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 4414 5
Size: 47 4362 2081
Fragments arranged by size:
4362 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6490
StyI C'CwwG_G
Cuts at: 513 1065 2004 2350 3929 513
Size: 552 939 346 1579 3074
Fragments arranged by size:
3074 1579 939 552 346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 4517 4534 4687 4872 5317 325
Size: 1255 480 2457 17 153 185 445 1498
Fragments arranged by size:
2457 1498 1255 480 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4736 6448 96
Size: 274 80 33 51 1443 2759 1712 138
Fragments arranged by size:
2759 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6450 6482 6450
Size: 32 6458
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6490
XmaI C'CCGG_G
Cuts at: 1870 3815 1870
Size: 1945 4545
XmnI GAAnn'nnTTC
Cuts at: 2627 2664 2832 3084 3215 3299 3571 3655 3739
Size: 37 168 252 131 84 272 84 84
Cuts at: 3739 4619 2627
Size: 880 4498
Fragments arranged by size:
4498 880 272 252 168 131 84 84
84 37
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI PspXI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI