Restriction map for
pFN21AB7807
(Circular) (Six-base) MAPSORT of: pFN21AB7807.seq Check: 9580 from: 1 to: 6280
pFN21AB7807 6280 bp
With 182 enzymes: SgfI *
October 20, 2009 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2675 2675
Size: 6280
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4090 278
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3623 1937
Size: 1686 4594
Acc65I G'GTAC_C
Cuts at: 2408 3601 2408
Size: 1193 5087
AclI AA'CG_TT
Cuts at: 1563 4407 4780 1563
Size: 2844 373 3063
Fragments arranged by size:
3063 2844 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2326 3126 3149 4343 5727 5995 1632
Size: 694 800 23 1194 1384 268 1917
Fragments arranged by size:
1917 1384 1194 800 694 268 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6083
AflIII A'CryG_T
Cuts at: 1203 5179 6156 1203
Size: 3976 977 1327
Fragments arranged by size:
3976 1327 977
AgeI A'CCGG_T
Cuts at: 5111 5111
Size: 6280
AhdI GACnn_n'nnGTC
Cuts at: 5009 5009
Size: 6280
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6280
AlwNI CAG_nnn'CTG
Cuts at: 2218 2357 3194 3379 3444 4023 5595 2218
Size: 139 837 185 65 579 1572 2903
Fragments arranged by size:
2903 1572 837 579 185 139 65
ApaLI G'TGCA_C
Cuts at: 4337 5493 4337
Size: 1156 5124
ApoI r'AATT_y
Cuts at: 1493 3589 3815 3967 6076 1493
Size: 2096 226 152 2109 1697
Fragments arranged by size:
2109 2096 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4834 160
Size: 872 3802 1606
Fragments arranged by size:
3802 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 3057 3605 1870
Size: 73 154 960 548 4545
Fragments arranged by size:
4545 960 548 154 73
BamHI G'GATC_C
Cuts at: 3610 3977 3610
Size: 367 5913
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2295 2408 3601 5056 618
Size: 325 217 627 508 113 1193 1455 1842
Fragments arranged by size:
1842 1455 1193 627 508 325 217 113
BanII G_rGCy'C
Cuts at: 1391 2340 2492 2964 3033 3092 3599 1391
Size: 949 152 472 69 59 507 4072
Fragments arranged by size:
4072 949 507 472 152 69 59
BbeI G_GCGC'C
Cuts at: 2299 2299
Size: 6280
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5309
BbvCI CC'TCA_GC
Cuts at: 3437 3564 3437
Size: 127 6153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2963 2997 4471 4505 1903
Size: 34 50 34 942 34 1474 34 3678
Fragments arranged by size:
3678 1474 942 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4172 5382 4172
Size: 1210 5070
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6280
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4891 136
Size: 107 122 71 631 605 3219 1525
Fragments arranged by size:
3219 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2391 6275 2391
Size: 3884 2396
BlpI GC'TnA_GC
Cuts at: 2948 3697 2948
Size: 749 5531
Bme1580I G_kGCm'C
Cuts at: 1790 2098 2470 2844 4341 5497 1790
Size: 308 372 374 1497 1156 2573
Fragments arranged by size:
2573 1497 1156 374 372 308
BmgBI CAC'GTC
Cuts at: 1318 2209 1318
Size: 891 5389
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2085 2673 2703
Size: 416 53 83 399 24 668 588 30
Cuts at: 2703 2980 3122 4011 4969 442
Size: 277 142 889 958 1753
Fragments arranged by size:
1753 958 889 668 588 416 399 277
142 83 53 30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 3047 3052 2632
Size: 415 5 5860
Fragments arranged by size:
5860 415 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 2356 2559 2735 3076 3248 4940 1239
Size: 1028 89 203 176 341 172 1692 2579
Fragments arranged by size:
2579 1692 1028 341 203 176 172 89
Bpu10I CC'TnA_GC
Cuts at: 3437 3564 3437
Size: 127 6153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 4405 5270 5568 2069
Size: 2336 865 298 2781
Fragments arranged by size:
2781 2336 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2174 4943 882
Size: 617 675 2769 2219
Fragments arranged by size:
2769 2219 675 617
BsaAI yAC'GTr
Cuts at: 493 1204 4071 493
Size: 711 2867 2702
Fragments arranged by size:
2867 2702 711
BsaBI GATnn'nnATC
Cuts at: 6274 6274
Size: 6280
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2296 3305 4087 4469
Size: 53 83 186 936 763 1009 782 382
Cuts at: 4469 5104 275
Size: 635 1451
Fragments arranged by size:
1451 1009 936 782 763 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2763 4713 5111 5385 5532 5943 2763
Size: 1950 398 274 147 411 3100
Fragments arranged by size:
3100 1950 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3062 3092 1178
Size: 30 165 30 1659 30 4366
Fragments arranged by size:
4366 1659 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2252 2613 2621 2661 2897 3245 1186
Size: 1063 3 361 8 40 236 348 4221
Fragments arranged by size:
4221 1063 361 348 236 40 8 3
BseYI C'CCAG_C
Cuts at: 2122 2669 2786 3221 5483 5979 2122
Size: 547 117 435 2262 496 2423
Fragments arranged by size:
2423 2262 547 496 435 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2231 2939 1163
Size: 573 495 708 4504
Fragments arranged by size:
4504 708 573 495
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 3730 4491 5519 1540
Size: 275 186 1423 306 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 306 275 186
BsiHKAI G_wGCw'C
Cuts at: 2573 2964 3033 3068 4341 4426 5497 2573
Size: 391 69 35 1273 85 1071 3356
Fragments arranged by size:
3356 1273 1071 391 85 69 35
BsmI GAATG_Cn'
Cuts at: 3791 3884 3791
Size: 93 6187
BsmBI CGTCTCn'nnnn_
Cuts at: 2374 3418 2374
Size: 1044 5236
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2340 2470 2492 2573 2844 2964
Size: 399 308 242 130 22 81 271 120
Cuts at: 2964 3033 3068 3092 3599 4341 4426 5497 1391
Size: 69 35 24 507 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 507 399 308 271 242
130 120 85 81 69 35 24 22
BspEI T'CCGG_A
Cuts at: 2763 5943 2763
Size: 3180 3100
BspHI T'CATG_A
Cuts at: 4169 5899 4169
Size: 1730 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2675 3330 3568 3635 844
Size: 1831 655 238 67 3489
Fragments arranged by size:
3489 1831 655 238 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2383 4167 1394
Size: 429 560 1784 3507
Fragments arranged by size:
3507 1784 560 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2437 3332 4775 4949 65
Size: 1183 1189 895 1443 174 1396
Fragments arranged by size:
1443 1396 1189 1183 895 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3573 4924 5111 1700
Size: 110 1763 1351 187 2869
Fragments arranged by size:
2869 1763 1351 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6280
BssHII G'CGCG_C
Cuts at: 1925 2155 1925
Size: 230 6050
BssSI C'ACGA_G
Cuts at: 2957 4340 5352 2957
Size: 1383 1012 3885
Fragments arranged by size:
3885 1383 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2465 794
Size: 1671 4609
BstBI TT'CG_AA
Cuts at: 5084 5084
Size: 6280
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6280
BstYI r'GATC_y
Cuts at: 1972 2391 2553 2757 2886 3610 3977 4362 4379
Size: 419 162 204 129 724 367 385 17
Cuts at: 4379 5831 5917 5929 6275 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 724 419 385 367 346 204
162 129 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2655 2655
Size: 6280
BtgI C'CryG_G
Cuts at: 513 1065 2103 3343 513
Size: 552 1038 1240 3450
Fragments arranged by size:
3450 1240 1038 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5541
Fragments arranged by size:
5541 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 2470 2519 3796 4590 4610 2108
Size: 362 49 1277 794 20 3778
Fragments arranged by size:
3778 1277 794 362 49 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6280
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6245
DraI TTT'AAA
Cuts at: 3584 3584
Size: 6280
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5287 809
Size: 507 3971 1802
Fragments arranged by size:
3971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 2558 2911
Size: 54 1425 50 275 18 441 287 353
Cuts at: 2911 3727 4616 6245 8
Size: 816 889 1629 43
Fragments arranged by size:
1629 1425 889 816 441 353 287 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3727 1537
Size: 275 1915 4090
Fragments arranged by size:
4090 1915 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2345 2390 2598 2874 3038 4210 1353
Size: 992 45 208 276 164 1172 3423
Fragments arranged by size:
3423 1172 992 276 208 164 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2061 4867 5241 5387 387
Size: 1064 138 472 2806 374 146 1280
Fragments arranged by size:
2806 1280 1064 472 374 146 138
EcoICRI GAG'CTC
Cuts at: 2962 3031 2962
Size: 69 6211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2326 2356 2559 2735 3076 3126
Size: 393 635 59 30 203 176 341 50
Cuts at: 3126 3149 3248 4343 4940 5727 5995 1239
Size: 23 99 1095 597 787 268 1524
Fragments arranged by size:
1524 1095 787 635 597 393 341 268
203 176 99 59 50 30 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2303 2538 3186 3230 3507 2303
Size: 235 648 44 277 5076
Fragments arranged by size:
5076 648 277 235 44
EcoO109I rG'GnC_Cy
Cuts at: 2146 2531 2545 3119 3191 3512 2146
Size: 385 14 574 72 321 4914
Fragments arranged by size:
4914 574 385 321 72 14
EcoRI G'AATT_C
Cuts at: 3589 3967 3589
Size: 378 5902
FspI TGC'GCA
Cuts at: 4786 4786
Size: 6280
HaeII r_GCGC'y
Cuts at: 1678 2018 2061 2299 3243 5427 1678
Size: 340 43 238 944 2184 2531
Fragments arranged by size:
2531 2184 944 340 238 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2498 2530 2783 2815 4921
Size: 32 135 32 926 32 253 32 2106
Cuts at: 4921 4953 4995 5027 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2106 926 253 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2348 3624 3870 669
Size: 988 281 410 1276 246 3079
Fragments arranged by size:
3079 1276 988 410 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6280
HpaI GTT'AAC
Cuts at: 3870 3870
Size: 6280
KasI G'GCGC_C
Cuts at: 2295 2295
Size: 6280
KpnI G_GTAC'C
Cuts at: 2412 3605 2412
Size: 1193 5087
MfeI C'AATT_G
Cuts at: 3879 3879
Size: 6280
MluI A'CGCG_T
Cuts at: 6156 6156
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2562 3130 5394 5578 1305
Size: 1257 568 2264 184 2007
Fragments arranged by size:
2264 2007 1257 568 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2273 2560 10
Size: 54 1425 343 441 287 3730
Fragments arranged by size:
3730 1425 441 343 287 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 4238 4597 4756 518
Size: 648 1572 1500 359 159 2042
Fragments arranged by size:
2042 1572 1500 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2218 3696 4373 5521 5766 1606
Size: 531 81 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 531 245 81
NaeI GCC'GGC
Cuts at: 3575 3575
Size: 6280
NarI GG'CG_CC
Cuts at: 2296 2296
Size: 6280
NcoI C'CATG_G
Cuts at: 513 1065 2103 3343 513
Size: 552 1038 1240 3450
Fragments arranged by size:
3450 1240 1038 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6280
NgoMIV G'CCGG_C
Cuts at: 3573 3573
Size: 6280
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6280
NotI GC'GGCC_GC
Cuts at: 3727 3727
Size: 6280
NspI r_CATG'y
Cuts at: 1132 1229 3638 5183 1132
Size: 97 2409 1545 2229
Fragments arranged by size:
2409 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2447 1348
Size: 1099 5181
PciI A'CATG_T
Cuts at: 5179 5179
Size: 6280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2525 3344 1108
Size: 1417 819 4044
Fragments arranged by size:
4044 1417 819
PmeI GTTT'AAAC
Cuts at: 3584 3584
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4349 4381 5862 5894 4349
Size: 32 1481 32 4735
Fragments arranged by size:
4735 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 2531 2545 3512 2146
Size: 385 14 967 4914
Fragments arranged by size:
4914 967 385 14
PshAI GACnn'nnGTC
Cuts at: 1613 3498 1613
Size: 1885 4395
PsiI TTA'TAA
Cuts at: 3850 3850
Size: 6280
PstI C_TGCA'G
Cuts at: 830 2076 2136 2154 2304 2641 3372 3632 830
Size: 1246 60 18 150 337 731 260 3478
Fragments arranged by size:
3478 1246 731 337 260 150 60 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6280
PvuII CAG'CTG
Cuts at: 2137 2218 2137
Size: 81 6199
SacI G_AGCT'C
Cuts at: 2964 3033 2964
Size: 69 6211
SalI G'TCGA_C
Cuts at: 1936 3622 1936
Size: 1686 4594
SanDI GG'GwC_CC
Cuts at: 2545 2545
Size: 6280
SapI GCTCTTCn'nnn_
Cuts at: 2345 2390 3038 2345
Size: 45 648 5587
Fragments arranged by size:
5587 648 45
SbfI CC_TGCA'GG
Cuts at: 2641 3632 2641
Size: 991 5289
ScaI AGT'ACT
Cuts at: 4528 6240 4528
Size: 1712 4568
SfcI C'TryA_G
Cuts at: 826 1045 2072 2132 2150 2300 2637 3368 3628
Size: 219 1027 60 18 150 337 731 260
Cuts at: 3628 4763 5444 5635 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 731 681 337 260 219
191 150 60 18
SfoI GGC'GCC
Cuts at: 2297 2297
Size: 6280
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6280
SmaI CCC'GGG
Cuts at: 1872 3607 1872
Size: 1735 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2084 4384 5285 5547 820
Size: 197 926 141 2300 901 262 1553
Fragments arranged by size:
2300 1553 926 901 262 197 141
SnaBI TAC'GTA
Cuts at: 493 4071 493
Size: 3578 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6280
SphI G_CATG'C
Cuts at: 1132 3638 1132
Size: 2506 3774
SspI AAT'ATT
Cuts at: 5 52 4204 5
Size: 47 4152 2081
Fragments arranged by size:
4152 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6280
StyI C'CwwG_G
Cuts at: 513 1065 2103 2774 3296 3343 3719 513
Size: 552 1038 671 522 47 376 3074
Fragments arranged by size:
3074 1038 671 552 522 376 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2724 4307 4324 4477 4662 5107 325
Size: 1255 1144 1583 17 153 185 445 1498
Fragments arranged by size:
1583 1498 1255 1144 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4526 6238 96
Size: 274 80 33 51 1443 2549 1712 138
Fragments arranged by size:
2549 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2683 2715 6240 6272 2683
Size: 32 3525 32 2691
Fragments arranged by size:
3525 2691 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6280
XbaI T'CTAG_A
Cuts at: 3616 3616
Size: 6280
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6280
XmaI C'CCGG_G
Cuts at: 1870 3605 1870
Size: 1735 4545
XmnI GAAnn'nnTTC
Cuts at: 4409 4409
Size: 6280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4088 276
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SexAI SfiI SrfI SwaI XcmI