Restriction map for
pFN21AB7803
(Circular) (Six-base) MAPSORT of: pFN21AB7803.seq Check: 3425 from: 1 to: 6214
pFN21AB7803 6214 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3415 3415
Size: 6214
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 1937
Size: 1620 4594
Acc65I G'GTAC_C
Cuts at: 3535 3535
Size: 6214
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2730 2991 4277 5661 5929 1632
Size: 1098 261 1286 1384 268 1917
Fragments arranged by size:
1917 1384 1286 1098 268 261
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 2330 5113 6090 1203
Size: 1127 2783 977 1327
Fragments arranged by size:
2783 1327 1127 977
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 2506 3290 4943 2506
Size: 784 1653 3777
Fragments arranged by size:
3777 1653 784
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6214
AlwNI CAG_nnn'CTG
Cuts at: 2195 2231 2591 3957 5529 2195
Size: 36 360 1366 1572 2880
Fragments arranged by size:
2880 1572 1366 360 36
ApaLI G'TGCA_C
Cuts at: 4271 5427 4271
Size: 1156 5058
ApoI r'AATT_y
Cuts at: 1493 2580 3523 3749 3901 6010 1493
Size: 1087 943 226 152 2109 1697
Fragments arranged by size:
2109 1697 1087 943 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 3539 1870
Size: 73 204 1392 4545
Fragments arranged by size:
4545 1392 204 73
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3535 4990 618
Size: 325 217 627 1748 1455 1842
Fragments arranged by size:
1842 1748 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3533 1391
Size: 2142 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 2505 2838 928
Size: 971 237 369 333 4304
Fragments arranged by size:
4304 971 369 333 237
BbvCI CC'TCA_GC
Cuts at: 3261 3261
Size: 6214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4405 4439 1903
Size: 34 2468 34 3678
Fragments arranged by size:
3678 2468 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2996 3143 3173
Size: 107 122 71 631 605 1324 147 30
Cuts at: 3173 3257 4825 136
Size: 84 1568 1525
Fragments arranged by size:
1568 1525 1324 631 605 147 122 107
84 71 30
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 4275 5431 1790
Size: 2485 1156 2573
Fragments arranged by size:
2573 2485 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3945 4903 442
Size: 416 53 83 399 24 2528 958 1753
Fragments arranged by size:
2528 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2566 2173
Size: 393 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2525 3354 4874 1239
Size: 1286 829 1520 2579
Fragments arranged by size:
2579 1520 1286 829
Bpu10I CC'TnA_GC
Cuts at: 3261 3261
Size: 6214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2627 4339 5204 5502 2627
Size: 1712 865 298 3339
Fragments arranged by size:
3339 1712 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4877 882
Size: 617 3378 2219
Fragments arranged by size:
3378 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4005 493
Size: 711 2801 2702
Fragments arranged by size:
2801 2702 711
BsaBI GATnn'nnATC
Cuts at: 3110 6208 3110
Size: 3098 3116
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4021 4403 5038 275
Size: 53 83 186 936 2488 382 635 1451
Fragments arranged by size:
2488 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2313 2733 4647 5045 5319 5466 5877 2313
Size: 420 1914 398 274 147 411 2650
Fragments arranged by size:
2650 1914 420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3192 3222 1178
Size: 30 165 30 1789 30 4170
Fragments arranged by size:
4170 1789 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6214
BseYI C'CCAG_C
Cuts at: 2067 2613 2673 5417 5913 2067
Size: 546 60 2744 496 2368
Fragments arranged by size:
2744 2368 546 496 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 2469 3443 1163
Size: 573 360 373 974 3934
Fragments arranged by size:
3934 974 573 373 360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3328 4275 4360 5431 3328
Size: 947 85 1071 4111
Fragments arranged by size:
4111 1071 947 85
BsmI GAATG_Cn'
Cuts at: 3725 3818 3725
Size: 93 6121
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3328 3533 4275 4360 5431 1391
Size: 399 1538 205 742 85 1071 2174
Fragments arranged by size:
2174 1538 1071 742 399 205 85
BspEI T'CCGG_A
Cuts at: 2313 2733 5877 2313
Size: 420 3144 2650
Fragments arranged by size:
3144 2650 420
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2071 2216 3415 3569 844
Size: 1227 145 1199 154 3489
Fragments arranged by size:
3489 1227 1199 154 145
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 1394
Size: 429 2278 3507
Fragments arranged by size:
3507 2278 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4709 4883 65
Size: 1183 3461 174 1396
Fragments arranged by size:
3461 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4858 5045 1700
Size: 110 3048 187 2869
Fragments arranged by size:
3048 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 2376 4274 5286 2376
Size: 1898 1012 3304
Fragments arranged by size:
3304 1898 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2113 794
Size: 1319 4895
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 3025 3391 1184
Size: 1841 366 4007
Fragments arranged by size:
4007 1841 366
BstYI r'GATC_y
Cuts at: 1972 3363 3544 3911 4296 4313 5765 5851 5863
Size: 1391 181 367 385 17 1452 86 12
Cuts at: 5863 6209 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1391 385 367 346 181 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3021 3054 3432 3474 513
Size: 552 939 1017 33 378 42 3253
Fragments arranged by size:
3253 1017 939 552 378 42 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5475
Fragments arranged by size:
5475 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 3177 3730 4524 4544 2067
Size: 1110 553 794 20 3737
Fragments arranged by size:
3737 1110 794 553 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 2437 3518 2437
Size: 1081 5133
DraIII CAC_nnn'GTG
Cuts at: 2020 2020
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3467 5221 809
Size: 507 2151 1754 1802
Fragments arranged by size:
2151 1802 1754 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 3429 3481
Size: 54 1425 50 275 18 411 1188 52
Cuts at: 3481 3661 4550 6179 8
Size: 180 889 1629 43
Fragments arranged by size:
1629 1425 1188 889 411 275 180 54
52 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 2748 4144 1353
Size: 959 436 1396 3423
Fragments arranged by size:
3423 1396 959 436
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4801 5175 5321 387
Size: 1064 138 3212 374 146 1280
Fragments arranged by size:
3212 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2525 2730 2991 3354 4277 4874 5661
Size: 393 893 205 261 363 923 597 787
Cuts at: 5661 5929 1239
Size: 268 1524
Fragments arranged by size:
1524 923 893 787 597 393 363 268
261 205
EcoNI CCTnn'n_nnAGG
Cuts at: 3260 3404 3260
Size: 144 6070
EcoO109I rG'GnC_Cy
Cuts at: 2228 2228
Size: 6214
EcoRI G'AATT_C
Cuts at: 2580 3523 3901 2580
Size: 943 378 4893
Fragments arranged by size:
4893 943 378
EcoRV GAT'ATC
Cuts at: 2716 3456 2716
Size: 740 5474
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 3221 5361 1678
Size: 1543 2140 2531
Fragments arranged by size:
2531 2140 1543
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2197 2229 2492
Size: 32 135 32 545 32 48 32 263
Cuts at: 2492 2524 3192 3224 3276 3308 4855 4887 4929
Size: 32 668 32 52 32 1547 32 42
Cuts at: 4929 4961 1373
Size: 32 2626
Fragments arranged by size:
2626 1547 668 545 263 135 52 48
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 3558 3804 669
Size: 988 281 610 1010 246 3079
Fragments arranged by size:
3079 1010 988 610 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 2548 3804 2548
Size: 1256 4958
KpnI G_GTAC'C
Cuts at: 3539 3539
Size: 6214
MfeI C'AATT_G
Cuts at: 3813 3813
Size: 6214
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3301 5328 5512 1305
Size: 1996 2027 184 2007
Fragments arranged by size:
2027 2007 1996 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3431 10
Size: 54 1425 343 1599 2793
Fragments arranged by size:
2793 1599 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4172 4531 4690 518
Size: 648 3006 359 159 2042
Fragments arranged by size:
3006 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2195 2222 2246 3630 4307 5455 5700 1606
Size: 589 27 24 1384 677 1148 245 2120
Fragments arranged by size:
2120 1384 1148 677 589 245 27 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 3432 3474 513
Size: 552 939 1428 42 3253
Fragments arranged by size:
3253 1428 939 552 42
NdeI CA'TA_TG
Cuts at: 387 2037 387
Size: 1650 4564
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 2060 3379 3572 5117 1132
Size: 97 831 1319 193 1545 2229
Fragments arranged by size:
2229 1545 1319 831 193 97
PasI CC'CwG_GG
Cuts at: 1348 2721 3406 1348
Size: 1373 685 4156
Fragments arranged by size:
4156 1373 685
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2703 3148 3388 3490 1108
Size: 1595 445 240 102 3832
Fragments arranged by size:
3832 1595 445 240 102
PfoI T'CCnGG_A
Cuts at: 2493 2493
Size: 6214
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2228 2228
Size: 6214
PshAI GACnn'nnGTC
Cuts at: 1613 2921 1613
Size: 1308 4906
PsiI TTA'TAA
Cuts at: 2814 3784 2814
Size: 970 5244
PstI C_TGCA'G
Cuts at: 830 2227 3566 830
Size: 1397 1339 3478
Fragments arranged by size:
3478 1397 1339
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 2195 2222 2195
Size: 27 6187
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SapI GCTCTTCn'nnn_
Cuts at: 2748 2748
Size: 6214
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 2648 4462 6174 2648
Size: 1814 1712 2688
Fragments arranged by size:
2688 1814 1712
SfcI C'TryA_G
Cuts at: 826 1045 2223 2652 2919 3204 3562 4697 5378
Size: 219 1178 429 267 285 358 1135 681
Cuts at: 5378 5569 826
Size: 191 1471
Fragments arranged by size:
1471 1178 1135 681 429 358 285 267
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 3541 1872
Size: 1669 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2642 4318 5219 5481 820
Size: 197 926 699 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 699 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3572 1132
Size: 2440 3774
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6214
StyI C'CwwG_G
Cuts at: 513 1065 2004 3432 3474 3653 513
Size: 552 939 1428 42 179 3074
Fragments arranged by size:
3074 1428 939 552 179 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3155 4241 4258 4411 4596 5041 325
Size: 1255 1575 1086 17 153 185 445 1498
Fragments arranged by size:
1575 1498 1255 1086 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2478 2646 4460
Size: 274 80 33 51 1443 501 168 1814
Cuts at: 4460 6172 96
Size: 1712 138
Fragments arranged by size:
1814 1712 1443 501 274 168 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6174 6206 6174
Size: 32 6182
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6214
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3145 3347 3145
Size: 202 6012
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6214
XmaI C'CCGG_G
Cuts at: 1870 3539 1870
Size: 1669 4545
XmnI GAAnn'nnTTC
Cuts at: 4343 4343
Size: 6214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI