Restriction map for
pFN21AB7802
(Circular) (Six-base) MAPSORT of: pFN21AB7802.seq Check: 2430 from: 1 to: 6475
pFN21AB7802 6475 bp
With 182 enzymes: SgfI *
March 28, 2012 12:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3678 3678
Size: 6475
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4285 278
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3818 1937
Size: 1881 4594
Acc65I G'GTAC_C
Cuts at: 3796 3796
Size: 6475
AclI AA'CG_TT
Cuts at: 1563 4602 4975 1563
Size: 3039 373 3063
Fragments arranged by size:
3063 3039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3533 4538 5922 6190 1632
Size: 1901 1005 1384 268 1917
Fragments arranged by size:
1917 1901 1384 1005 268
AfeI AGC'GCT
Cuts at: 2388 2738 2388
Size: 350 6125
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6278
AflIII A'CryG_T
Cuts at: 1203 2868 5374 6351 1203
Size: 1665 2506 977 1327
Fragments arranged by size:
2506 1665 1327 977
AgeI A'CCGG_T
Cuts at: 5306 5306
Size: 6475
AhdI GACnn_n'nnGTC
Cuts at: 5204 5204
Size: 6475
AleI CACnn'nnGTG
Cuts at: 1166 2708 1166
Size: 1542 4933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3773 1184
Size: 2589 3886
AlwNI CAG_nnn'CTG
Cuts at: 2416 2440 3164 3679 4218 5790 2416
Size: 24 724 515 539 1572 3101
Fragments arranged by size:
3101 1572 724 539 515 24
ApaI G_GGCC'C
Cuts at: 2839 2994 3692 3744 2839
Size: 155 698 52 5570
Fragments arranged by size:
5570 698 155 52
ApaLI G'TGCA_C
Cuts at: 3613 4532 5688 3613
Size: 919 1156 4400
Fragments arranged by size:
4400 1156 919
ApoI r'AATT_y
Cuts at: 1493 2477 3784 4010 4162 6271 1493
Size: 984 1307 226 152 2109 1697
Fragments arranged by size:
2109 1697 1307 984 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 2093 2684 2861 3182 3298 3409 3479
Size: 73 150 591 177 321 116 111 70
Cuts at: 3479 3551 3736 3800 1870
Size: 72 185 64 4545
Fragments arranged by size:
4545 591 321 185 177 150 116 111
73 72 70 64
BamHI G'GATC_C
Cuts at: 3051 3448 3520 3805 4172 3051
Size: 397 72 285 367 5354
Fragments arranged by size:
5354 397 367 285 72
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2110 2231 2317 2710
Size: 325 217 627 279 44 121 86 393
Cuts at: 2710 2826 3544 3623 3749 3796 5251 618
Size: 116 718 79 126 47 1455 1842
Fragments arranged by size:
1842 1455 718 627 393 325 279 217
126 121 116 86 79 47 44
BanII G_rGCy'C
Cuts at: 1391 2839 2994 3182 3312 3405 3692 3744 3794
Size: 1448 155 188 130 93 287 52 50
Cuts at: 3794 1391
Size: 4072
Fragments arranged by size:
4072 1448 287 188 155 130 93 52
50
BbeI G_GCGC'C
Cuts at: 2070 2114 2235 2321 2830 3548 2070
Size: 44 121 86 509 718 4997
Fragments arranged by size:
4997 718 509 121 86 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2395 2542 2914 928
Size: 971 496 147 372 4489
Fragments arranged by size:
4489 971 496 372 147
BbvCI CC'TCA_GC
Cuts at: 2273 2273
Size: 6475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2533 2567 4666 4700 1903
Size: 34 596 34 2099 34 3678
Fragments arranged by size:
3678 2099 596 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4367 5577 4367
Size: 1210 5265
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2329 2376
Size: 107 122 71 631 605 337 320 47
Cuts at: 2376 2888 3161 3275 5086 136
Size: 512 273 114 1811 1525
Fragments arranged by size:
1811 1525 631 605 512 337 320 273
122 114 107 71 47
BglII A'GATC_T
Cuts at: 6470 6470
Size: 6475
BlpI GC'TnA_GC
Cuts at: 2892 3218 3892 2892
Size: 326 674 5475
Fragments arranged by size:
5475 674 326
Bme1580I G_kGCm'C
Cuts at: 1790 2715 2839 2868 2994 3617 3692 3744 3752
Size: 925 124 29 126 623 75 52 8
Cuts at: 3752 4536 5692 1790
Size: 784 1156 2573
Fragments arranged by size:
2573 1156 925 784 623 126 124 75
52 29 8
BmgBI CAC'GTC
Cuts at: 1318 2788 1318
Size: 1470 5005
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2606 2832 4206
Size: 416 53 83 399 24 1189 226 1374
Cuts at: 4206 5164 442
Size: 958 1753
Fragments arranged by size:
1753 1374 1189 958 416 399 226 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 3469 3505 2518
Size: 951 36 5488
Fragments arranged by size:
5488 951 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2224 2609 3439 5135 1239
Size: 974 11 385 830 1696 2579
Fragments arranged by size:
2579 1696 974 830 385 11
Bpu10I CC'TnA_GC
Cuts at: 2273 2273
Size: 6475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4600 5465 5763 4600
Size: 865 298 5312
Fragments arranged by size:
5312 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2398 2522 2945 5138 882
Size: 617 899 124 423 2193 2219
Fragments arranged by size:
2219 2193 899 617 423 124
BsaAI yAC'GTr
Cuts at: 493 1204 2818 3067 3214 4266 493
Size: 711 1614 249 147 1052 2702
Fragments arranged by size:
2702 1614 1052 711 249 147
BsaBI GATnn'nnATC
Cuts at: 6469 6469
Size: 6475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2111 2232 2318
Size: 53 83 186 936 534 44 121 86
Cuts at: 2318 2609 2615 2827 3042 3545 4282 4664 5299
Size: 291 6 212 215 503 737 382 635
Cuts at: 5299 275
Size: 1451
Fragments arranged by size:
1451 936 737 635 534 503 382 291
215 212 186 121 86 83 53 44
6
BsaWI w'CCGG_w
Cuts at: 2117 3523 4908 5306 5580 5727 6138 2117
Size: 1406 1385 398 274 147 411 2454
Fragments arranged by size:
2454 1406 1385 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6250
Fragments arranged by size:
6250 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2635 2644 2647 2710 3299 1186
Size: 1449 9 3 63 589 4362
Fragments arranged by size:
4362 1449 589 63 9 3
BseYI C'CCAG_C
Cuts at: 2311 3164 3352 3649 3679 5678 6174 2311
Size: 853 188 297 30 1999 496 2612
Fragments arranged by size:
2612 1999 853 496 297 188 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2768 3560 1163
Size: 573 1032 792 4078
Fragments arranged by size:
4078 1032 792 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3925 4686 5714 1540
Size: 275 186 1924 761 1028 2301
Fragments arranged by size:
2301 1924 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3192 3219 3617 4536 4621 5692 3192
Size: 27 398 919 85 1071 3975
Fragments arranged by size:
3975 1071 919 398 85 27
BsmI GAATG_Cn'
Cuts at: 3986 4079 3986
Size: 93 6382
BsmBI CGTCTCn'nnnn_
Cuts at: 2647 2933 2647
Size: 286 6189
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2715 2839 2868 2994 3182 3192 3219
Size: 399 925 124 29 126 188 10 27
Cuts at: 3219 3312 3405 3617 3692 3744 3752 3794 4536
Size: 93 93 212 75 52 8 42 742
Cuts at: 4536 4621 5692 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 925 742 399 212 188 126
124 93 93 85 75 52 42 29
27 10 8
BspEI T'CCGG_A
Cuts at: 2117 6138 2117
Size: 4021 2454
BspHI T'CATG_A
Cuts at: 4364 6094 4364
Size: 1730 4745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3678 3830 844
Size: 2834 152 3489
Fragments arranged by size:
3489 2834 152
BsrBI CCG'CTC
Cuts at: 1394 1823 3171 4362 1394
Size: 429 1348 1191 3507
Fragments arranged by size:
3507 1348 1191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4970 5144 65
Size: 1183 3722 174 1396
Fragments arranged by size:
3722 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2298 3595 5119 5306 1700
Size: 110 488 1297 1524 187 2869
Fragments arranged by size:
2869 1524 1297 488 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6475
BssHII G'CGCG_C
Cuts at: 1925 2165 1925
Size: 240 6235
BssSI C'ACGA_G
Cuts at: 3046 4535 5547 3046
Size: 1489 1012 3974
Fragments arranged by size:
3974 1489 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2872 3196 794
Size: 2078 324 4073
Fragments arranged by size:
4073 2078 324
BstBI TT'CG_AA
Cuts at: 5279 5279
Size: 6475
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6475
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2512 2822 2248
Size: 264 310 5901
Fragments arranged by size:
5901 310 264
BstYI r'GATC_y
Cuts at: 1972 3051 3448 3520 3805 4172 4557 4574 6026
Size: 1079 397 72 285 367 385 17 1452
Cuts at: 6026 6112 6124 6470 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1079 397 385 367 346 285
86 72 17 12
Bsu36I CC'TnA_GG
Cuts at: 2797 2797
Size: 6475
BtgI C'CryG_G
Cuts at: 513 1065 2004 2062 2340 2374 2905 3031 3533
Size: 552 939 58 278 34 531 126 502
Cuts at: 3533 3642 3744 3761 513
Size: 109 102 17 3227
Fragments arranged by size:
3227 939 552 531 502 278 126 109
102 58 34 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5736
Fragments arranged by size:
5736 683 56
BtsI GCAGTG_nn'
Cuts at: 2032 3991 4785 4805 2032
Size: 1959 794 20 3702
Fragments arranged by size:
3702 1959 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6440
DraI TTT'AAA
Cuts at: 3779 3779
Size: 6475
DraIII CAC_nnn'GTG
Cuts at: 3196 3196
Size: 6475
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5482 809
Size: 507 4166 1802
Fragments arranged by size:
4166 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 3153 3235
Size: 54 1425 50 275 18 378 945 82
Cuts at: 3235 3465 3530 3922 4811 6440 8
Size: 230 65 392 889 1629 43
Fragments arranged by size:
1629 1425 945 889 392 378 275 230
82 65 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3922 1537
Size: 275 2110 4090
Fragments arranged by size:
4090 2110 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2653 2665 2671 3170 4405 1353
Size: 1300 12 6 499 1235 3423
Fragments arranged by size:
3423 1300 1235 499 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2049 2279 3044 5062 5436 5582
Size: 1064 138 460 230 765 2018 374 146
Cuts at: 5582 387
Size: 1280
Fragments arranged by size:
2018 1280 1064 765 460 374 230 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2224 2609 3439 3533 4538 5135
Size: 393 581 11 385 830 94 1005 597
Cuts at: 5135 5922 6190 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1005 830 787 597 581 393 385
268 94 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2705 2729 3515 2705
Size: 24 786 5665
Fragments arranged by size:
5665 786 24
EcoO109I rG'GnC_Cy
Cuts at: 2990 2991 3367 3508 3538 3556 3607 3689 3740
Size: 1 376 141 30 18 51 82 51
Cuts at: 3740 2990
Size: 5725
Fragments arranged by size:
5725 376 141 82 51 51 30 18
1
EcoRI G'AATT_C
Cuts at: 3784 4162 3784
Size: 378 6097
FspI TGC'GCA
Cuts at: 4981 4981
Size: 6475
HaeII r_GCGC'y
Cuts at: 1678 2070 2114 2235 2321 2390 2740 2830 3548
Size: 392 44 121 86 69 350 90 718
Cuts at: 3548 5622 1678
Size: 2074 2531
Fragments arranged by size:
2531 2074 718 392 350 121 90 86
69 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2412 2444 2901 2933 3275
Size: 32 135 32 840 32 457 32 342
Cuts at: 3275 3307 3667 3699 5116 5148 5190 5222 1373
Size: 32 360 32 1417 32 42 32 2626
Fragments arranged by size:
2626 1417 840 457 360 342 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3819 4065 669
Size: 988 281 1881 246 3079
Fragments arranged by size:
3079 1881 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2213 748
Size: 1465 5010
HpaI GTT'AAC
Cuts at: 4065 4065
Size: 6475
KasI G'GCGC_C
Cuts at: 2066 2110 2231 2317 2826 3544 2066
Size: 44 121 86 509 718 4997
Fragments arranged by size:
4997 718 509 121 86 44
KpnI G_GTAC'C
Cuts at: 3800 3800
Size: 6475
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 6351 6351
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3353 5589 5773 1305
Size: 2048 2236 184 2007
Fragments arranged by size:
2236 2048 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 3155 3237 10
Size: 54 1425 343 378 945 82 3248
Fragments arranged by size:
3248 1425 945 378 343 82 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2708 2816 3395 4433 4792 4951 518
Size: 648 1542 108 579 1038 359 159 2042
Fragments arranged by size:
2042 1542 1038 648 579 359 159 108
MspA1I CmG'CkG
Cuts at: 1606 2064 2224 2248 2342 2354 2376 3015 3356
Size: 458 160 24 94 12 22 639 341
Cuts at: 3356 3644 3746 3763 3891 4568 5716 5961 1606
Size: 288 102 17 128 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 639 458 341 288 245
160 128 102 94 24 22 17 12
NarI GG'CG_CC
Cuts at: 2067 2111 2232 2318 2827 3545 2067
Size: 44 121 86 509 718 4997
Fragments arranged by size:
4997 718 509 121 86 44
NcoI C'CATG_G
Cuts at: 513 1065 2004 2905 3533 513
Size: 552 939 901 628 3455
Fragments arranged by size:
3455 939 901 628 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 3922 3922
Size: 6475
NspI r_CATG'y
Cuts at: 1132 1229 3833 5378 1132
Size: 97 2604 1545 2229
Fragments arranged by size:
2604 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2079 2325 2567 2845 3379 3721 1348
Size: 731 246 242 278 534 342 4102
Fragments arranged by size:
4102 731 534 342 278 246 242
PciI A'CATG_T
Cuts at: 5374 5374
Size: 6475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2845 2906 1108
Size: 1737 61 4677
Fragments arranged by size:
4677 1737 61
PfoI T'CCnGG_A
Cuts at: 2153 2730 3699 2153
Size: 577 969 4929
Fragments arranged by size:
4929 969 577
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PmlI CAC'GTG
Cuts at: 2818 3067 3214 2818
Size: 249 147 6079
Fragments arranged by size:
6079 249 147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4544 4576 6057 6089 4544
Size: 32 1481 32 4930
Fragments arranged by size:
4930 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3508 3556 3508
Size: 48 6427
PshAI GACnn'nnGTC
Cuts at: 1613 3084 1613
Size: 1471 5004
PsiI TTA'TAA
Cuts at: 4045 4045
Size: 6475
PspOMI G'GGCC_C
Cuts at: 2835 2990 3688 3740 2835
Size: 155 698 52 5570
Fragments arranged by size:
5570 698 155 52
PstI C_TGCA'G
Cuts at: 830 2133 3120 3147 3675 3827 830
Size: 1303 987 27 528 152 3478
Fragments arranged by size:
3478 1303 987 528 152 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
PvuII CAG'CTG
Cuts at: 2248 2354 3015 2248
Size: 106 661 5708
Fragments arranged by size:
5708 661 106
RsrII CG'GwC_CG
Cuts at: 2120 2120
Size: 6475
SacII CC_GC'GG
Cuts at: 2065 2343 2377 3645 3747 3764 2065
Size: 278 34 1268 102 17 4776
Fragments arranged by size:
4776 1268 278 102 34 17
SalI G'TCGA_C
Cuts at: 1936 3817 1936
Size: 1881 4594
SanDI GG'GwC_CC
Cuts at: 3556 3556
Size: 6475
SapI GCTCTTCn'nnn_
Cuts at: 3170 3170
Size: 6475
SbfI CC_TGCA'GG
Cuts at: 2133 3827 2133
Size: 1694 4781
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SfcI C'TryA_G
Cuts at: 826 1045 2129 3116 3143 3671 3823 4958 5639
Size: 219 1084 987 27 528 152 1135 681
Cuts at: 5639 5830 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1084 987 681 528 219 191
152 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 2376 2376
Size: 6475
SfoI GGC'GCC
Cuts at: 2068 2112 2233 2319 2828 3546 2068
Size: 44 121 86 509 718 4997
Fragments arranged by size:
4997 718 509 121 86 44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 3595 1700
Size: 1895 4580
SmaI CCC'GGG
Cuts at: 1872 2095 3184 3300 3553 3738 3802 1872
Size: 223 1089 116 253 185 64 4545
Fragments arranged by size:
4545 1089 253 223 185 116 64
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4579 5480 5742 820
Size: 197 926 2636 901 262 1553
Fragments arranged by size:
2636 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4266 493
Size: 3773 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6475
SphI G_CATG'C
Cuts at: 1132 3833 1132
Size: 2701 3774
SspI AAT'ATT
Cuts at: 5 52 4399 5
Size: 47 4347 2081
Fragments arranged by size:
4347 2081 47
StuI AGG'CCT
Cuts at: 1517 2795 3707 1517
Size: 1278 912 4285
Fragments arranged by size:
4285 1278 912
StyI C'CwwG_G
Cuts at: 513 1065 2004 2706 2905 3533 3914 513
Size: 552 939 702 199 628 381 3074
Fragments arranged by size:
3074 939 702 628 552 381 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4502 4519 4672 4857 5302 325
Size: 1255 2922 17 153 185 445 1498
Fragments arranged by size:
2922 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4721 6433 96
Size: 274 80 33 51 1443 2744 1712 138
Fragments arranged by size:
2744 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6435 6467 6435
Size: 32 6443
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6475
XbaI T'CTAG_A
Cuts at: 3811 3811
Size: 6475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3230 3230
Size: 6475
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6475
XmaI C'CCGG_G
Cuts at: 1870 2093 3182 3298 3551 3736 3800 1870
Size: 223 1089 116 253 185 64 4545
Fragments arranged by size:
4545 1089 253 223 185 116 64
XmnI GAAnn'nnTTC
Cuts at: 4604 4604
Size: 6475
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4283 276
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI SacI SexAI SrfI SwaI