Restriction map for
pFN21AB7795
(Circular) (Six-base) MAPSORT of: pFN21AB7795.seq Check: 2989 from: 1 to: 6088
pFN21AB7795 6088 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3431 1937
Size: 1494 4594
Acc65I G'GTAC_C
Cuts at: 2264 3409 2264
Size: 1145 4943
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2136 2279 2352 2415 3395 4151 5535 5803
Size: 504 143 73 63 980 756 1384 268
Cuts at: 5803 1632
Size: 1917
Fragments arranged by size:
1917 1384 980 756 504 268 143 73
63
AfeI AGC'GCT
Cuts at: 2849 2849
Size: 6088
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 3224 4987 5964 1203
Size: 2021 1763 977 1327
Fragments arranged by size:
2021 1763 1327 977
AgeI A'CCGG_T
Cuts at: 2551 4919 2551
Size: 2368 3720
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2141 2534 3831 5403 2141
Size: 393 1297 1572 2826
Fragments arranged by size:
2826 1572 1297 393
ApaI G_GGCC'C
Cuts at: 3059 3059
Size: 6088
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 3397 3623 3775 5884 1493
Size: 1904 226 152 2109 1697
Fragments arranged by size:
2109 1904 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2161 2750 2831 3142 3413 1870
Size: 73 218 589 81 311 271 4545
Fragments arranged by size:
4545 589 311 271 218 81 73
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2264 2745 2922 3099 3409
Size: 325 217 627 477 481 177 177 310
Cuts at: 3409 4864 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 481 477 325 310 217
177 177
BanII G_rGCy'C
Cuts at: 1391 2074 2274 2564 2579 2821 2961 3059 3407
Size: 683 200 290 15 242 140 98 348
Cuts at: 3407 1391
Size: 4072
Fragments arranged by size:
4072 683 348 290 242 200 140 98
15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2142 928
Size: 971 243 4874
Fragments arranged by size:
4874 971 243
BbvCI CC'TCA_GC
Cuts at: 2461 2648 2461
Size: 187 5901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2785 2819 3139 3173 4279 4313 1903
Size: 34 848 34 320 34 1106 34 3678
Fragments arranged by size:
3678 1106 848 320 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2228 2688 4699
Size: 107 122 71 631 605 556 460 2011
Cuts at: 4699 136
Size: 1525
Fragments arranged by size:
2011 1525 631 605 556 460 122 107
71
BglII A'GATC_T
Cuts at: 6083 6083
Size: 6088
BlpI GC'TnA_GC
Cuts at: 3086 3505 3086
Size: 419 5669
Bme1580I G_kGCm'C
Cuts at: 1790 2915 3059 4149 5305 1790
Size: 1125 144 1090 1156 2573
Fragments arranged by size:
2573 1156 1125 1090 144
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3819 4777 442
Size: 416 53 83 399 24 2402 958 1753
Fragments arranged by size:
2402 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2492 4748 1239
Size: 818 435 2256 2579
Fragments arranged by size:
2579 2256 818 435
Bpu10I CC'TnA_GC
Cuts at: 2461 2648 2461
Size: 187 5901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4751 882
Size: 617 3252 2219
Fragments arranged by size:
3252 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3225 3879 493
Size: 711 2021 654 2702
Fragments arranged by size:
2702 2021 711 654
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2551 4521 4919 5193 5340 5751 2551
Size: 1970 398 274 147 411 2888
Fragments arranged by size:
2888 1970 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2375 2400 2405 2430 1178
Size: 30 165 30 972 25 5 25 4836
Fragments arranged by size:
4836 972 165 30 30 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2233 1186
Size: 1047 5041
BseYI C'CCAG_C
Cuts at: 2411 2626 2711 5291 5787 2411
Size: 215 85 2580 496 2712
Fragments arranged by size:
2712 2580 496 215 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5515
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 3538 4299 5327 1540
Size: 275 186 1246 291 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 291 275 186
BsiHKAI G_wGCw'C
Cuts at: 2564 2757 3165 4149 4234 5305 2564
Size: 193 408 984 85 1071 3347
Fragments arranged by size:
3347 1071 984 408 193 85
BsmI GAATG_Cn'
Cuts at: 3131 3599 3692 3131
Size: 468 93 5527
Fragments arranged by size:
5527 468 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2436 2436
Size: 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2274 2564 2579 2757 2821 2915
Size: 399 284 200 290 15 178 64 94
Cuts at: 2915 2961 3059 3165 3407 4149 4234 5305 1391
Size: 46 98 106 242 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 290 284 242 200
178 106 98 94 85 64 46 15
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 3977 5707 3977
Size: 1730 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 844
Size: 2599 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3316 3975 1394
Size: 429 1493 659 3507
Fragments arranged by size:
3507 1493 659 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2551 3242 3344 4732 4919 1700
Size: 110 741 691 102 1388 187 2869
Fragments arranged by size:
2869 1388 741 691 187 110 102
BsrGI T'GTAC_A
Cuts at: 96 2181 96
Size: 2085 4003
BssHII G'CGCG_C
Cuts at: 1925 3090 3340 1925
Size: 1165 250 4673
Fragments arranged by size:
4673 1165 250
BssSI C'ACGA_G
Cuts at: 2981 3164 4148 5160 2981
Size: 183 984 1012 3909
Fragments arranged by size:
3909 1012 984 183
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2193 3126 3303 794
Size: 1399 933 177 3579
Fragments arranged by size:
3579 1399 933 177
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 2899 1184
Size: 1715 4373
BstXI CCAn_nnnn'nTGG
Cuts at: 2785 2785
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 3418 3785 4170 4187 5639 5725 5737 6083
Size: 1446 367 385 17 1452 86 12 346
Cuts at: 6083 1972
Size: 1977
Fragments arranged by size:
1977 1452 1446 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4597
Fragments arranged by size:
4597 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 3604 4398 4418 3604
Size: 794 20 5274
Fragments arranged by size:
5274 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3113 5095 809
Size: 507 1797 1982 1802
Fragments arranged by size:
1982 1802 1797 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2119 3244 3535
Size: 54 1425 50 275 18 289 1125 291
Cuts at: 3535 4424 6053 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1125 889 291 289 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 3535 1537
Size: 275 1432 291 4090
Fragments arranged by size:
4090 1432 291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2251 3386 4018 1353
Size: 898 1135 632 3423
Fragments arranged by size:
3423 1135 898 632
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2562 2562
Size: 6088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2136 2279 2352 2415 2492 3395
Size: 393 425 79 143 73 63 77 903
Cuts at: 3395 4151 4748 5535 5803 1239
Size: 756 597 787 268 1524
Fragments arranged by size:
1524 903 787 756 597 425 393 268
143 79 77 73 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2444 2444
Size: 6088
EcoO109I rG'GnC_Cy
Cuts at: 2011 3055 3056 3327 2011
Size: 1044 1 271 4772
Fragments arranged by size:
4772 1044 271 1
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3346 3346
Size: 6088
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 2086 2851 3086 5235 1678
Size: 408 765 235 2149 2531
Fragments arranged by size:
2531 2149 765 408 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2400 2407 2432 4729
Size: 32 135 32 803 25 7 25 2297
Cuts at: 4729 4761 4803 4835 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2297 803 135 42 32 32 32
32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KpnI G_GTAC'C
Cuts at: 2268 3413 2268
Size: 1145 4943
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2667 2911 5202 5386 1305
Size: 1362 244 2291 184 2007
Fragments arranged by size:
2291 2007 1362 244 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2121 10
Size: 54 1425 343 289 3977
Fragments arranged by size:
3977 1425 343 289 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4046 4405 4564 518
Size: 648 2880 359 159 2042
Fragments arranged by size:
2880 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2253 3025 3249 3504 4181 5329 5574 1606
Size: 647 772 224 255 677 1148 245 2120
Fragments arranged by size:
2120 1148 772 677 647 255 245 224
NaeI GCC'GGC
Cuts at: 3346 3346
Size: 6088
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4597
Fragments arranged by size:
4597 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NgoMIV G'CCGG_C
Cuts at: 3344 3344
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2059 3297 3446 4991 1132
Size: 97 830 1238 149 1545 2229
Fragments arranged by size:
2229 1545 1238 830 149 97
PasI CC'CwG_GG
Cuts at: 1348 2785 3262 1348
Size: 1437 477 4174
Fragments arranged by size:
4174 1437 477
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2117 2534 2864 1108
Size: 1009 417 330 4332
Fragments arranged by size:
4332 1009 417 330
PfoI T'CCnGG_A
Cuts at: 3063 3333 3063
Size: 270 5818
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PmlI CAC'GTG
Cuts at: 3225 3225
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 3327 2011
Size: 1316 4772
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 3055 3055
Size: 6088
PstI C_TGCA'G
Cuts at: 830 2221 2640 3024 3122 3440 830
Size: 1391 419 384 98 318 3478
Fragments arranged by size:
3478 1391 419 384 318 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 3025 3025
Size: 6088
SacI G_AGCT'C
Cuts at: 2564 2564
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SapI GCTCTTCn'nnn_
Cuts at: 3386 3386
Size: 6088
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2217 2636 3020 3118 3436 4571 5252
Size: 219 1172 419 384 98 318 1135 681
Cuts at: 5252 5443 826
Size: 191 1471
Fragments arranged by size:
1471 1172 1135 681 419 384 318 219
191 98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 2551 1700
Size: 851 5237
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4192 5093 5355 820
Size: 197 926 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 2682 1517
Size: 1165 4923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2060 2907 3321 3527 513
Size: 552 939 56 847 414 206 3074
Fragments arranged by size:
3074 939 847 552 414 206 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2193 2684 2940 4115 4132 4285 4470
Size: 1255 613 491 256 1175 17 153 185
Cuts at: 4470 4915 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1175 613 491 445 256 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 4334 6046
Size: 274 80 33 51 1443 204 2153 1712
Cuts at: 6046 96
Size: 138
Fragments arranged by size:
2153 1712 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6048 6080 6048
Size: 32 6056
Tth111I GACn'n_nGTC
Cuts at: 1316 2137 1316
Size: 821 5267
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2257 2473 2011
Size: 246 216 5626
Fragments arranged by size:
5626 246 216
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 3191 3380 4217 3191
Size: 189 837 5062
Fragments arranged by size:
5062 837 189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI