Restriction map for
pFN21AB7792
(Circular) (Six-base) MAPSORT of: pFN21AB7792.seq Check: 9034 from: 1 to: 6262
pFN21AB7792 6262 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3605 1937
Size: 1668 4594
Acc65I G'GTAC_C
Cuts at: 3583 3583
Size: 6262
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4325 5709 5977 1632
Size: 2693 1384 268 1917
Fragments arranged by size:
2693 1917 1384 268
AfeI AGC'GCT
Cuts at: 2669 2669
Size: 6262
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 2028 3483 5161 6138 1203
Size: 825 1455 1678 977 1327
Fragments arranged by size:
1678 1455 1327 977 825
AgeI A'CCGG_T
Cuts at: 3507 5093 3507
Size: 1586 4676
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 2610 1166
Size: 1444 4818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6262
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2421 2453 2421
Size: 32 6230
AlwNI CAG_nnn'CTG
Cuts at: 4005 5577 4005
Size: 1572 4690
ApaI G_GGCC'C
Cuts at: 2391 2391
Size: 6262
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 2846 3571 3797 3949 6058 1493
Size: 1353 725 226 152 2109 1697
Fragments arranged by size:
2109 1697 1353 725 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 2105 2125 2266 3007 3172 3587 1870
Size: 73 162 20 141 741 165 415 4545
Fragments arranged by size:
4545 741 415 165 162 141 73 20
BamHI G'GATC_C
Cuts at: 3592 3959 3592
Size: 367 5895
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3074 3148 3583 5038 618
Size: 325 217 627 1287 74 435 1455 1842
Fragments arranged by size:
1842 1455 1287 627 435 325 217 74
BanII G_rGCy'C
Cuts at: 1391 2094 2391 2834 3581 1391
Size: 703 297 443 747 4072
Fragments arranged by size:
4072 747 703 443 297
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2763 928
Size: 971 864 4427
Fragments arranged by size:
4427 971 864
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4453 4487 1903
Size: 34 2516 34 3678
Fragments arranged by size:
3678 2516 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6262
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2703 4873 136
Size: 107 122 71 631 605 1031 2170 1525
Fragments arranged by size:
2170 1525 1031 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2783 6257 2783
Size: 3474 2788
BlpI GC'TnA_GC
Cuts at: 3679 3679
Size: 6262
Bme1580I G_kGCm'C
Cuts at: 1790 2391 2708 3077 3153 4323 5479 1790
Size: 601 317 369 76 1170 1156 2573
Fragments arranged by size:
2573 1170 1156 601 369 317 76
BmgBI CAC'GTC
Cuts at: 1318 3427 1318
Size: 2109 4153
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2711 3993 4951
Size: 416 53 83 399 24 1294 1282 958
Cuts at: 4951 442
Size: 1753
Fragments arranged by size:
1753 1294 1282 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 6262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 2445 4922 1239
Size: 819 387 2477 2579
Fragments arranged by size:
2579 2477 819 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3272 4387 5252 5550 3272
Size: 1115 865 298 3984
Fragments arranged by size:
3984 1115 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3239 4925 882
Size: 617 1740 1686 2219
Fragments arranged by size:
2219 1740 1686 617
BsaAI yAC'GTr
Cuts at: 493 1204 2932 4053 493
Size: 711 1728 1121 2702
Fragments arranged by size:
2702 1728 1121 711
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2562 3002 3167 4069
Size: 53 83 186 936 1029 440 165 902
Cuts at: 4069 4451 5086 275
Size: 382 635 1451
Fragments arranged by size:
1451 1029 936 902 635 440 382 186
165 83 53
BsaWI w'CCGG_w
Cuts at: 3507 4695 5093 5367 5514 5925 3507
Size: 1188 398 274 147 411 3844
Fragments arranged by size:
3844 1188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6037
Fragments arranged by size:
6037 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3233 3308 1186
Size: 2047 75 4140
Fragments arranged by size:
4140 2047 75
BseYI C'CCAG_C
Cuts at: 5465 5961 5465
Size: 496 5766
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3017 3038 1163
Size: 573 1281 21 4387
Fragments arranged by size:
4387 1281 573 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3712 4473 5501 1540
Size: 275 186 1711 761 1028 2301
Fragments arranged by size:
2301 1711 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2400 2834 3300 3414 3546 4323 4408 5479 2400
Size: 434 466 114 132 777 85 1071 3183
Fragments arranged by size:
3183 1071 777 466 434 132 114 85
BsmI GAATG_Cn'
Cuts at: 2259 2331 2503 3773 3866 2259
Size: 72 172 1270 93 4655
Fragments arranged by size:
4655 1270 172 93 72
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2391 2400 2708 2834 3077 3153
Size: 399 304 297 9 308 126 243 76
Cuts at: 3153 3300 3414 3546 3581 4323 4408 5479 1391
Size: 147 114 132 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 308 304 297 243
147 132 126 114 85 76 35 9
BspEI T'CCGG_A
Cuts at: 5925 5925
Size: 6262
BspHI T'CATG_A
Cuts at: 4151 5881 4151
Size: 1730 4532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2490 3129 3321 3617 844
Size: 1646 639 192 296 3489
Fragments arranged by size:
3489 1646 639 296 192
BsrBI CCG'CTC
Cuts at: 1394 1823 3064 4149 1394
Size: 429 1241 1085 3507
Fragments arranged by size:
3507 1241 1085 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4757 4931 65
Size: 1183 3509 174 1396
Fragments arranged by size:
3509 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3507 4906 5093 1700
Size: 110 1697 1399 187 2869
Fragments arranged by size:
2869 1697 1399 187 110
BsrGI T'GTAC_A
Cuts at: 96 3387 3432 96
Size: 3291 45 2926
Fragments arranged by size:
3291 2926 45
BssHII G'CGCG_C
Cuts at: 1925 3269 3319 1925
Size: 1344 50 4868
Fragments arranged by size:
4868 1344 50
BssSI C'ACGA_G
Cuts at: 2789 4322 5334 2789
Size: 1533 1012 3717
Fragments arranged by size:
3717 1533 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2451 3183 3486 794
Size: 1657 732 303 3570
Fragments arranged by size:
3570 1657 732 303
BstBI TT'CG_AA
Cuts at: 5066 5066
Size: 6262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6262
BstXI CCAn_nnnn'nTGG
Cuts at: 3445 3445
Size: 6262
BstYI r'GATC_y
Cuts at: 1972 2783 3592 3959 4344 4361 5813 5899 5911
Size: 811 809 367 385 17 1452 86 12
Cuts at: 5911 6257 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 811 809 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2579 2579
Size: 6262
BtgI C'CryG_G
Cuts at: 513 1065 2004 3111 3195 3454 513
Size: 552 939 1107 84 259 3321
Fragments arranged by size:
3321 1107 939 552 259 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2100 3241 487
Size: 683 56 874 1141 3508
Fragments arranged by size:
3508 1141 874 683 56
BtsI GCAGTG_nn'
Cuts at: 3400 3778 4572 4592 3400
Size: 378 794 20 5070
Fragments arranged by size:
5070 794 378 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6227
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5269 809
Size: 507 3953 1802
Fragments arranged by size:
3953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2572 2623
Size: 54 1425 50 275 18 333 409 51
Cuts at: 2623 3709 4598 6227 8
Size: 1086 889 1629 43
Fragments arranged by size:
1629 1425 1086 889 409 333 275 54
51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3709 1537
Size: 275 1897 4090
Fragments arranged by size:
4090 1897 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2193 3419 4192 1353
Size: 840 1226 773 3423
Fragments arranged by size:
3423 1226 840 773
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4849 5223 5369 387
Size: 1064 138 3260 374 146 1280
Fragments arranged by size:
3260 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2832 2832
Size: 6262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2445 4325 4922 5709 5977 1239
Size: 393 426 387 1880 597 787 268 1524
Fragments arranged by size:
1880 1524 787 597 426 393 387 268
EcoO109I rG'GnC_Cy
Cuts at: 2110 2457 2863 2110
Size: 347 406 5509
Fragments arranged by size:
5509 406 347
EcoRI G'AATT_C
Cuts at: 3571 3949 3571
Size: 378 5884
EcoRV GAT'ATC
Cuts at: 2365 2365
Size: 6262
FspI TGC'GCA
Cuts at: 4768 4768
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 2671 2895 3111 5409 1678
Size: 993 224 216 2298 2531
Fragments arranged by size:
2531 2298 993 224 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3506 3538 4903 4935 4977
Size: 32 135 32 1934 32 1365 32 42
Cuts at: 4977 5009 1373
Size: 32 2626
Fragments arranged by size:
2626 1934 1365 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3606 3852 669
Size: 988 281 1668 246 3079
Fragments arranged by size:
3079 1668 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6262
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KpnI G_GTAC'C
Cuts at: 3587 3587
Size: 6262
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2305 2895 3111 5376 5560 1305
Size: 1000 590 216 2265 184 2007
Fragments arranged by size:
2265 2007 1000 590 216 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2165 2574 10
Size: 54 1425 343 333 409 3698
Fragments arranged by size:
3698 1425 409 343 333 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2610 3443 4220 4579 4738 518
Size: 648 1444 833 777 359 159 2042
Fragments arranged by size:
2042 1444 833 777 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2992 3052 3231 3369 3678 4355 5503 5748
Size: 1386 60 179 138 309 677 1148 245
Cuts at: 5748 1606
Size: 2120
Fragments arranged by size:
2120 1386 1148 677 309 245 179 138
60
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4771
Fragments arranged by size:
4771 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 2032 3487 3620 5165 1132
Size: 97 803 1455 133 1545 2229
Fragments arranged by size:
2229 1545 1455 803 133 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 2028 3483 5161 2028
Size: 1455 1678 3129
Fragments arranged by size:
3129 1678 1455
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2935 1108
Size: 1827 4435
PfoI T'CCnGG_A
Cuts at: 3417 3417
Size: 6262
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PmlI CAC'GTG
Cuts at: 2932 2932
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4331 4363 5844 5876 4331
Size: 32 1481 32 4717
Fragments arranged by size:
4717 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 2110
Size: 6262
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6262
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PspOMI G'GGCC_C
Cuts at: 2387 2387
Size: 6262
PstI C_TGCA'G
Cuts at: 830 3614 830
Size: 2784 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
PvuII CAG'CTG
Cuts at: 3052 3052
Size: 6262
SacI G_AGCT'C
Cuts at: 2834 2834
Size: 6262
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SapI GCTCTTCn'nnn_
Cuts at: 3419 3419
Size: 6262
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SfcI C'TryA_G
Cuts at: 826 1045 3610 4745 5426 5617 826
Size: 219 2565 1135 681 191 1471
Fragments arranged by size:
2565 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 3009 3589 1872
Size: 1137 580 4545
Fragments arranged by size:
4545 1137 580
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3287 4366 5267 5529 820
Size: 197 926 1344 1079 901 262 1553
Fragments arranged by size:
1553 1344 1079 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 3620 1132
Size: 2488 3774
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6262
StyI C'CwwG_G
Cuts at: 513 1065 2004 2299 3135 3701 513
Size: 552 939 295 836 566 3074
Fragments arranged by size:
3074 939 836 566 552 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2483 4289 4306 4459 4644 5089 325
Size: 1255 903 1806 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 903 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3387 3432 4508
Size: 274 80 33 51 1443 1410 45 1076
Cuts at: 4508 6220 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1410 1076 274 138 80 51
45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6222 6254 6222
Size: 32 6230
Tth111I GACn'n_nGTC
Cuts at: 1316 2156 1316
Size: 840 5422
XbaI T'CTAG_A
Cuts at: 3598 3598
Size: 6262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2965 3142 2398
Size: 567 177 5518
Fragments arranged by size:
5518 567 177
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 3007 3587 1870
Size: 1137 580 4545
Fragments arranged by size:
4545 1137 580
XmnI GAAnn'nnTTC
Cuts at: 2519 4391 2519
Size: 1872 4390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI