Restriction map for
pFN21AB7784
(Circular) (Six-base) MAPSORT of: pFN21AB7784.seq Check: 1650 from: 1 to: 6235
pFN21AB7784 6235 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4045 278
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3578 1937
Size: 1641 4594
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 3534 4362 4735 1563
Size: 1971 828 373 3063
Fragments arranged by size:
3063 1971 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 3109 4298 5682 5950 1632
Size: 566 911 1189 1384 268 1917
Fragments arranged by size:
1917 1384 1189 911 566 268
AfeI AGC'GCT
Cuts at: 2146 2146
Size: 6235
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6038
AflIII A'CryG_T
Cuts at: 1203 3437 5134 6111 1203
Size: 2234 1697 977 1327
Fragments arranged by size:
2234 1697 1327 977
AgeI A'CCGG_T
Cuts at: 5066 5066
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 4964 4964
Size: 6235
AleI CACnn'nnGTG
Cuts at: 1166 3420 1166
Size: 2254 3981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6235
AlwNI CAG_nnn'CTG
Cuts at: 2236 2296 2365 3978 5550 2236
Size: 60 69 1613 1572 2921
Fragments arranged by size:
2921 1613 1572 69 60
ApaLI G'TGCA_C
Cuts at: 4292 5448 4292
Size: 1156 5079
ApoI r'AATT_y
Cuts at: 1493 2378 3159 3327 3544 3770 3922 6031 1493
Size: 885 781 168 217 226 152 2109 1697
Fragments arranged by size:
2109 1697 885 781 226 217 168 152
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 3560 1870
Size: 73 1617 4545
Fragments arranged by size:
4545 1617 73
BamHI G'GATC_C
Cuts at: 3565 3932 3565
Size: 367 5868
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3556 5011 618
Size: 325 217 627 1769 1455 1842
Fragments arranged by size:
1842 1769 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2502 3554 1391
Size: 1111 1052 4072
Fragments arranged by size:
4072 1111 1052
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2780 928
Size: 971 881 4383
Fragments arranged by size:
4383 971 881
BbvCI CC'TCA_GC
Cuts at: 2237 2237
Size: 6235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4426 4460 1903
Size: 34 2489 34 3678
Fragments arranged by size:
3678 2489 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4127 5337 4127
Size: 1210 5025
BclI T'GATC_A
Cuts at: 1548 2617 2857 1548
Size: 1069 240 4926
Fragments arranged by size:
4926 1069 240
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4846 136
Size: 107 122 71 631 605 3174 1525
Fragments arranged by size:
3174 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2265 3223 6230 2265
Size: 958 3007 2270
Fragments arranged by size:
3007 2270 958
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 4296 5452 1790
Size: 2506 1156 2573
Fragments arranged by size:
2573 2506 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2335 3966 4924
Size: 416 53 83 399 24 918 1631 958
Cuts at: 4924 442
Size: 1753
Fragments arranged by size:
1753 1631 958 918 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 6235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2077 2182 3189 4895 1239
Size: 838 105 1007 1706 2579
Fragments arranged by size:
2579 1706 1007 838 105
Bpu10I CC'TnA_GC
Cuts at: 2237 2297 2237
Size: 60 6175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2023 2216 2789 4360 5225 5523 2023
Size: 193 573 1571 865 298 2735
Fragments arranged by size:
2735 1571 865 573 298 193
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2309 4898 882
Size: 617 810 2589 2219
Fragments arranged by size:
2589 2219 810 617
BsaAI yAC'GTr
Cuts at: 493 1204 4026 493
Size: 711 2822 2702
Fragments arranged by size:
2822 2702 711
BsaBI GATnn'nnATC
Cuts at: 6229 6229
Size: 6235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4042 4424 5059 275
Size: 53 83 186 936 2509 382 635 1451
Fragments arranged by size:
2509 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2186 4668 5066 5340 5487 5898 2186
Size: 2482 398 274 147 411 2523
Fragments arranged by size:
2523 2482 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2155 2185 1178
Size: 30 165 30 752 30 5228
Fragments arranged by size:
5228 752 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6235
BseYI C'CCAG_C
Cuts at: 2431 2563 2589 5438 5934 2431
Size: 132 26 2849 496 2732
Fragments arranged by size:
2849 2732 496 132 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 1163
Size: 573 615 5047
Fragments arranged by size:
5047 615 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3473 3685 4446 5474 1540
Size: 275 186 1472 212 761 1028 2301
Fragments arranged by size:
2301 1472 1028 761 275 212 186
BsiHKAI G_wGCw'C
Cuts at: 2451 4296 4381 5452 2451
Size: 1845 85 1071 3234
Fragments arranged by size:
3234 1845 1071 85
BsmI GAATG_Cn'
Cuts at: 2301 3746 3839 2301
Size: 1445 93 4697
Fragments arranged by size:
4697 1445 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2451 2502 3554 4296 4381 5452 1391
Size: 399 661 51 1052 742 85 1071 2174
Fragments arranged by size:
2174 1071 1052 742 661 399 85 51
BspEI T'CCGG_A
Cuts at: 5898 5898
Size: 6235
BspHI T'CATG_A
Cuts at: 2176 3240 4124 5854 2176
Size: 1064 884 1730 2557
Fragments arranged by size:
2557 1730 1064 884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3034 3590 844
Size: 2190 556 3489
Fragments arranged by size:
3489 2190 556
BsrBI CCG'CTC
Cuts at: 1394 1823 4122 1394
Size: 429 2299 3507
Fragments arranged by size:
3507 2299 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4730 4904 65
Size: 1183 3482 174 1396
Fragments arranged by size:
3482 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4879 5066 1700
Size: 110 3069 187 2869
Fragments arranged by size:
3069 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2980 96
Size: 2884 3351
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6235
BssSI C'ACGA_G
Cuts at: 4295 5307 4295
Size: 1012 5223
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6235
BstBI TT'CG_AA
Cuts at: 5039 5039
Size: 6235
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6235
BstYI r'GATC_y
Cuts at: 1972 2247 2265 3061 3223 3565 3932 4317 4334
Size: 275 18 796 162 342 367 385 17
Cuts at: 4334 5786 5872 5884 6230 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 796 385 367 346 342 275
162 86 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4744
Fragments arranged by size:
4744 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2024 487
Size: 683 56 798 4698
Fragments arranged by size:
4698 798 683 56
BtsI GCAGTG_nn'
Cuts at: 2451 2472 3751 4545 4565 2451
Size: 21 1279 794 20 4121
Fragments arranged by size:
4121 1279 794 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6200
DraI TTT'AAA
Cuts at: 3352 3539 3352
Size: 187 6048
DraIII CAC_nnn'GTG
Cuts at: 2978 2978
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2074 5242 809
Size: 507 758 3168 1802
Fragments arranged by size:
3168 1802 758 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3682 4571 6200
Size: 54 1425 50 275 18 1852 889 1629
Cuts at: 6200 8
Size: 43
Fragments arranged by size:
1852 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3682 1537
Size: 275 1870 4090
Fragments arranged by size:
4090 1870 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2739 4165 1353
Size: 1386 1426 3423
Fragments arranged by size:
3423 1426 1386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4822 5196 5342 387
Size: 1064 138 3233 374 146 1280
Fragments arranged by size:
3233 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 2182 2198 3109 3189 4298 4895
Size: 393 445 105 16 911 80 1109 597
Cuts at: 4895 5682 5950 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1109 911 787 597 445 393 268
105 80 16
EcoO109I rG'GnC_Cy
Cuts at: 3077 3077
Size: 6235
EcoRI G'AATT_C
Cuts at: 2378 3159 3327 3544 3922 2378
Size: 781 168 217 378 4691
Fragments arranged by size:
4691 781 378 217 168
EcoRV GAT'ATC
Cuts at: 2803 2803
Size: 6235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3475 3475
Size: 6235
FspI TGC'GCA
Cuts at: 4741 4741
Size: 6235
HaeII r_GCGC'y
Cuts at: 1678 2148 5382 1678
Size: 470 3234 2531
Fragments arranged by size:
3234 2531 470
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 3208 3240 4876
Size: 32 135 32 449 32 1155 32 1636
Cuts at: 4876 4908 4950 4982 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1636 1155 449 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2709 3191 3579 3825 669
Size: 988 281 771 482 388 246 3079
Fragments arranged by size:
3079 988 771 482 388 281 246
HindIII A'AGCT_T
Cuts at: 748 3121 3289 748
Size: 2373 168 3694
Fragments arranged by size:
3694 2373 168
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 3834 3834
Size: 6235
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5349 5533 1305
Size: 4044 184 2007
Fragments arranged by size:
4044 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2899 3268 3420 4193 4552 4711
Size: 648 843 890 369 152 773 359 159
Cuts at: 4711 518
Size: 2042
Fragments arranged by size:
2042 890 843 773 648 369 359 159
152
MspA1I CmG'CkG
Cuts at: 1606 2563 3651 4328 5476 5721 1606
Size: 957 1088 677 1148 245 2120
Fragments arranged by size:
2120 1148 1088 957 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4744
Fragments arranged by size:
4744 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6235
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 3441 3519 3593 5138 1132
Size: 97 2212 78 74 1545 2229
Fragments arranged by size:
2229 2212 1545 97 78 74
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6235
PciI A'CATG_T
Cuts at: 3437 5134 3437
Size: 1697 4538
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2431 2509 1108
Size: 1323 78 4834
Fragments arranged by size:
4834 1323 78
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PstI C_TGCA'G
Cuts at: 830 3587 830
Size: 2757 3478
PvuI CG_AT'CG
Cuts at: 2001 3473 2001
Size: 1472 4763
PvuII CAG'CTG
Cuts at: 2563 2563
Size: 6235
RsrII CG'GwC_CG
Cuts at: 2189 2189
Size: 6235
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 4483 6195 4483
Size: 1712 4523
SfcI C'TryA_G
Cuts at: 826 1045 3583 4718 5399 5590 826
Size: 219 2538 1135 681 191 1471
Fragments arranged by size:
2538 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6235
SmaI CCC'GGG
Cuts at: 1872 3562 1872
Size: 1690 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 2195 2768 4339 5240 5502
Size: 197 926 95 157 573 1571 901 262
Cuts at: 5502 820
Size: 1553
Fragments arranged by size:
1571 1553 926 901 573 262 197 157
95
SnaBI TAC'GTA
Cuts at: 493 4026 493
Size: 3533 2702
SpeI A'CTAG_T
Cuts at: 152 2069 152
Size: 1917 4318
SphI G_CATG'C
Cuts at: 1132 3593 1132
Size: 2461 3774
SspI AAT'ATT
Cuts at: 5 52 2531 4159 5
Size: 47 2479 1628 2081
Fragments arranged by size:
2479 2081 1628 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6235
StyI C'CwwG_G
Cuts at: 513 1065 2004 2310 3005 3674 513
Size: 552 939 306 695 669 3074
Fragments arranged by size:
3074 939 695 669 552 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2317 2501 4262 4279 4432 4617 5062
Size: 1255 737 184 1761 17 153 185 445
Cuts at: 5062 325
Size: 1498
Fragments arranged by size:
1761 1498 1255 737 445 185 184 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2980 4481 6193
Size: 274 80 33 51 1443 1003 1501 1712
Cuts at: 6193 96
Size: 138
Fragments arranged by size:
1712 1501 1443 1003 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2451 2483 6195 6227 2451
Size: 32 3712 32 2459
Fragments arranged by size:
3712 2459 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2481 2481
Size: 6235
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 3560 1870
Size: 1690 4545
XmnI GAAnn'nnTTC
Cuts at: 3122 4364 3122
Size: 1242 4993
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4043 276
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI