Restriction map for
pFN21AB7783
(Circular) (Six-base) MAPSORT of: pFN21AB7783.seq Check: 5923 from: 1 to: 6076
pFN21AB7783 6076 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3886 278
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2364 3419 1937
Size: 427 1055 4594
Fragments arranged by size:
4594 1055 427
Acc65I G'GTAC_C
Cuts at: 3397 3397
Size: 6076
AclI AA'CG_TT
Cuts at: 1563 3375 4203 4576 1563
Size: 1812 828 373 3063
Fragments arranged by size:
3063 1812 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 2406 4139 5523 5791 1632
Size: 545 229 1733 1384 268 1917
Fragments arranged by size:
1917 1733 1384 545 268 229
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5879
AflIII A'CryG_T
Cuts at: 1203 4975 5952 1203
Size: 3772 977 1327
Fragments arranged by size:
3772 1327 977
AgeI A'CCGG_T
Cuts at: 4907 4907
Size: 6076
AhdI GACnn_n'nnGTC
Cuts at: 4805 4805
Size: 6076
AleI CACnn'nnGTG
Cuts at: 1166 2520 3198 1166
Size: 1354 678 4044
Fragments arranged by size:
4044 1354 678
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6076
AlwNI CAG_nnn'CTG
Cuts at: 2498 2698 2930 3819 5391 2498
Size: 200 232 889 1572 3183
Fragments arranged by size:
3183 1572 889 232 200
ApaLI G'TGCA_C
Cuts at: 4133 5289 4133
Size: 1156 4920
ApoI r'AATT_y
Cuts at: 1493 2965 3028 3385 3611 3763 5872 1493
Size: 1472 63 357 226 152 2109 1697
Fragments arranged by size:
2109 1697 1472 357 226 152 63
AseI AT'TA_AT
Cuts at: 160 1032 4630 160
Size: 872 3598 1606
Fragments arranged by size:
3598 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
AvaI C'yCGr_G
Cuts at: 1870 1943 3401 1870
Size: 73 1458 4545
Fragments arranged by size:
4545 1458 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3202 3235 3202
Size: 33 6043
BamHI G'GATC_C
Cuts at: 3406 3773 3406
Size: 367 5709
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 2108 3290 3366 3397
Size: 325 217 627 275 46 1182 76 31
Cuts at: 3397 4852 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1182 627 325 275 217 76
46 31
BanII G_rGCy'C
Cuts at: 1391 3395 1391
Size: 2004 4072
BbeI G_GCGC'C
Cuts at: 2066 2112 3294 2066
Size: 46 1182 4848
Fragments arranged by size:
4848 1182 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2410 3156 928
Size: 971 511 746 3848
Fragments arranged by size:
3848 971 746 511
BbvCI CC'TCA_GC
Cuts at: 2298 2298
Size: 6076
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4267 4301 1903
Size: 34 2330 34 3678
Fragments arranged by size:
3678 2330 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3968 5178 3968
Size: 1210 4866
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6076
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4687 136
Size: 107 122 71 631 605 3015 1525
Fragments arranged by size:
3015 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2650 6071 2650
Size: 3421 2655
BlpI GC'TnA_GC
Cuts at: 2021 3493 2021
Size: 1472 4604
Bme1580I G_kGCm'C
Cuts at: 1790 2052 4137 5293 1790
Size: 262 2085 1156 2573
Fragments arranged by size:
2573 2085 1156 262
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6076
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2718 3807 4765
Size: 416 53 83 399 24 1301 1089 958
Cuts at: 4765 442
Size: 1753
Fragments arranged by size:
1753 1301 1089 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 2292 2589 2945 3259 4736 1239
Size: 993 60 297 356 314 1477 2579
Fragments arranged by size:
2579 1477 993 356 314 297 60
Bpu10I CC'TnA_GC
Cuts at: 2298 2298
Size: 6076
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2078 4201 5066 5364 2078
Size: 2123 865 298 2790
Fragments arranged by size:
2790 2123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2461 4739 882
Size: 617 962 2278 2219
Fragments arranged by size:
2278 2219 962 617
BsaAI yAC'GTr
Cuts at: 493 1204 3867 493
Size: 711 2663 2702
Fragments arranged by size:
2702 2663 711
BsaBI GATnn'nnATC
Cuts at: 2655 6070 2655
Size: 3415 2661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2063 2109 3291 3883
Size: 53 83 186 936 530 46 1182 592
Cuts at: 3883 4265 4900 275
Size: 382 635 1451
Fragments arranged by size:
1451 1182 936 635 592 530 382 186
83 53 46
BsaWI w'CCGG_w
Cuts at: 2707 3276 4509 4907 5181 5328 5739 2707
Size: 569 1233 398 274 147 411 3044
Fragments arranged by size:
3044 1233 569 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2521 2551 2587 2617 2962
Size: 30 165 30 1118 30 36 30 345
Cuts at: 2962 2992 1178
Size: 30 4262
Fragments arranged by size:
4262 1118 345 165 36 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2529 1186
Size: 979 364 4733
Fragments arranged by size:
4733 979 364
BseYI C'CCAG_C
Cuts at: 2052 2104 2217 2673 5279 5775 2052
Size: 52 113 456 2606 496 2353
Fragments arranged by size:
2606 2353 496 456 113 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5503
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3254 3526 4287 5315 1540
Size: 275 186 1253 272 761 1028 2301
Fragments arranged by size:
2301 1253 1028 761 275 272 186
BsiHKAI G_wGCw'C
Cuts at: 4137 4222 5293 4137
Size: 85 1071 4920
Fragments arranged by size:
4920 1071 85
BsmI GAATG_Cn'
Cuts at: 3587 3680 3587
Size: 93 5983
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 3395 4137 4222 5293 1391
Size: 399 262 1343 742 85 1071 2174
Fragments arranged by size:
2174 1343 1071 742 399 262 85
BspEI T'CCGG_A
Cuts at: 5739 5739
Size: 6076
BspHI T'CATG_A
Cuts at: 3965 5695 3965
Size: 1730 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3431 844
Size: 2587 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3963 1394
Size: 429 2140 3507
Fragments arranged by size:
3507 2140 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2754 4571 4745 65
Size: 1183 1506 1817 174 1396
Fragments arranged by size:
1817 1506 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 2077 2098 2212 2231 4720 4907
Size: 110 249 18 21 114 19 2489 187
Cuts at: 4907 1700
Size: 2869
Fragments arranged by size:
2869 2489 249 187 114 110 21 19
18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6076
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6076
BssSI C'ACGA_G
Cuts at: 4136 5148 4136
Size: 1012 5064
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6076
BstBI TT'CG_AA
Cuts at: 4880 4880
Size: 6076
BstEII G'GTnAC_C
Cuts at: 1184 2391 1184
Size: 1207 4869
BstYI r'GATC_y
Cuts at: 1972 2650 2710 3406 3773 4158 4175 5627 5713
Size: 678 60 696 367 385 17 1452 86
Cuts at: 5713 5725 6071 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 696 678 385 367 346 86
60 17 12
Bsu36I CC'TnA_GG
Cuts at: 3185 3185
Size: 6076
BtgI C'CryG_G
Cuts at: 513 1065 2004 2237 2781 513
Size: 552 939 233 544 3808
Fragments arranged by size:
3808 939 552 544 233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5337
Fragments arranged by size:
5337 683 56
BtsI GCAGTG_nn'
Cuts at: 2832 3592 4386 4406 2832
Size: 760 794 20 4502
Fragments arranged by size:
4502 794 760 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6076
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6041
DraI TTT'AAA
Cuts at: 3174 3380 3174
Size: 206 5870
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5083 809
Size: 507 3767 1802
Fragments arranged by size:
3767 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3523 4412 6041
Size: 54 1425 50 275 18 1693 889 1629
Cuts at: 6041 8
Size: 43
Fragments arranged by size:
1693 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3523 1537
Size: 275 1711 4090
Fragments arranged by size:
4090 1711 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 3356 4006 1353
Size: 791 1212 650 3423
Fragments arranged by size:
3423 1212 791 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4663 5037 5183 387
Size: 1064 138 3074 374 146 1280
Fragments arranged by size:
3074 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2232 2292 2406 2589 2945 3259
Size: 393 545 55 60 114 183 356 314
Cuts at: 3259 4139 4736 5523 5791 1239
Size: 880 597 787 268 1524
Fragments arranged by size:
1524 880 787 597 545 393 356 314
268 183 114 60 55
EcoO109I rG'GnC_Cy
Cuts at: 2042 2203 2254 2557 2042
Size: 161 51 303 5561
Fragments arranged by size:
5561 303 161 51
EcoRI G'AATT_C
Cuts at: 3385 3763 3385
Size: 378 5698
FspI TGC'GCA
Cuts at: 4582 4582
Size: 6076
HaeII r_GCGC'y
Cuts at: 1678 2034 2066 2112 2138 3294 5223 1678
Size: 356 32 46 26 1156 1929 2531
Fragments arranged by size:
2531 1929 1156 356 46 32 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2339 2371 2521 2553 2587
Size: 32 135 32 767 32 150 32 34
Cuts at: 2587 2619 4717 4749 4791 4823 1373
Size: 32 2098 32 42 32 2626
Fragments arranged by size:
2626 2098 767 150 135 42 34 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3052 3420 3666 669
Size: 988 281 1114 368 246 3079
Fragments arranged by size:
3079 1114 988 368 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6076
HpaI GTT'AAC
Cuts at: 3052 3666 3052
Size: 614 5462
KasI G'GCGC_C
Cuts at: 2062 2108 3290 2062
Size: 46 1182 4848
Fragments arranged by size:
4848 1182 46
KpnI G_GTAC'C
Cuts at: 3401 3401
Size: 6076
MfeI C'AATT_G
Cuts at: 2997 3675 2997
Size: 678 5398
MluI A'CGCG_T
Cuts at: 5952 5952
Size: 6076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3091 5190 5374 1305
Size: 1786 2099 184 2007
Fragments arranged by size:
2099 2007 1786 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4254
Fragments arranged by size:
4254 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2520 3198 3214 4034 4393 4552 518
Size: 648 1354 678 16 820 359 159 2042
Fragments arranged by size:
2042 1354 820 678 648 359 159 16
MspA1I CmG'CkG
Cuts at: 1606 2239 2777 3492 4169 5317 5562 1606
Size: 633 538 715 677 1148 245 2120
Fragments arranged by size:
2120 1148 715 677 633 538 245
NaeI GCC'GGC
Cuts at: 2061 2214 2061
Size: 153 5923
NarI GG'CG_CC
Cuts at: 2063 2109 3291 2063
Size: 46 1182 4848
Fragments arranged by size:
4848 1182 46
NcoI C'CATG_G
Cuts at: 513 1065 2004 2781 513
Size: 552 939 777 3808
Fragments arranged by size:
3808 939 777 552
NdeI CA'TA_TG
Cuts at: 387 3357 387
Size: 2970 3106
NgoMIV G'CCGG_C
Cuts at: 2059 2212 2059
Size: 153 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6076
NotI GC'GGCC_GC
Cuts at: 3523 3523
Size: 6076
NspI r_CATG'y
Cuts at: 1132 1229 2848 3434 4979 1132
Size: 97 1619 586 1545 2229
Fragments arranged by size:
2229 1619 1545 586 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6076
PciI A'CATG_T
Cuts at: 4975 4975
Size: 6076
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6076
PmeI GTTT'AAAC
Cuts at: 3380 3380
Size: 6076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2066 2098 4145 4177 5658 5690 2066
Size: 32 2047 32 1481 32 2452
Fragments arranged by size:
2452 2047 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2042 2254 2042
Size: 212 5864
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6076
PsiI TTA'TAA
Cuts at: 3646 3646
Size: 6076
PstI C_TGCA'G
Cuts at: 830 2827 3428 830
Size: 1997 601 3478
Fragments arranged by size:
3478 1997 601
PvuI CG_AT'CG
Cuts at: 2001 3254 2001
Size: 1253 4823
PvuII CAG'CTG
Cuts at: 2777 2777
Size: 6076
SacII CC_GC'GG
Cuts at: 2240 2240
Size: 6076
SalI G'TCGA_C
Cuts at: 1936 3418 1936
Size: 1482 4594
SanDI GG'GwC_CC
Cuts at: 2042 2042
Size: 6076
SapI GCTCTTCn'nnn_
Cuts at: 2144 2144
Size: 6076
SbfI CC_TGCA'GG
Cuts at: 3428 3428
Size: 6076
ScaI AGT'ACT
Cuts at: 4324 6036 4324
Size: 1712 4364
SexAI A'CCwGG_T
Cuts at: 2256 2256
Size: 6076
SfcI C'TryA_G
Cuts at: 826 1045 2823 3424 4559 5240 5431 826
Size: 219 1778 601 1135 681 191 1471
Fragments arranged by size:
1778 1471 1135 681 601 219 191
SfoI GGC'GCC
Cuts at: 2064 2110 3292 2064
Size: 46 1182 4848
Fragments arranged by size:
4848 1182 46
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
SgrAI Cr'CCGG_yG
Cuts at: 1700 2059 1700
Size: 359 5717
SmaI CCC'GGG
Cuts at: 1872 3403 1872
Size: 1531 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2093 4180 5081 5343 820
Size: 197 926 150 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197 150
SnaBI TAC'GTA
Cuts at: 493 3867 493
Size: 3374 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6076
SphI G_CATG'C
Cuts at: 1132 2848 3434 1132
Size: 1716 586 3774
Fragments arranged by size:
3774 1716 586
SspI AAT'ATT
Cuts at: 5 52 2620 4000 5
Size: 47 2568 1380 2081
Fragments arranged by size:
2568 2081 1380 47
StuI AGG'CCT
Cuts at: 1517 2790 1517
Size: 1273 4803
StyI C'CwwG_G
Cuts at: 513 1065 2004 2781 3515 513
Size: 552 939 777 734 3074
Fragments arranged by size:
3074 939 777 734 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4103 4120 4273 4458 4903 325
Size: 1255 2523 17 153 185 445 1498
Fragments arranged by size:
2523 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4322 6034 96
Size: 274 80 33 51 1443 2345 1712 138
Fragments arranged by size:
2345 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2887 2919 3048 3080 6036 6068 2887
Size: 32 129 32 2956 32 2895
Fragments arranged by size:
2956 2895 129 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2258 1316
Size: 942 5134
XbaI T'CTAG_A
Cuts at: 2713 3412 2713
Size: 699 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6076
XmaI C'CCGG_G
Cuts at: 1870 3401 1870
Size: 1531 4545
XmnI GAAnn'nnTTC
Cuts at: 2417 2834 4205 2417
Size: 417 1371 4288
Fragments arranged by size:
4288 1371 417
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3884 276
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SfiI SrfI SwaI
XcmI