Restriction map for
pFN21AB7779
(Circular) (Six-base) MAPSORT of: pFN21AB7779.seq Check: 4179 from: 1 to: 6007
pFN21AB7779 6007 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 1937
Size: 1413 4594
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 3306 4134 4507 1563
Size: 1743 828 373 3063
Fragments arranged by size:
3063 1743 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2230 2415 2713 4070 5454 5722 1632
Size: 598 185 298 1357 1384 268 1917
Fragments arranged by size:
1917 1384 1357 598 298 268 185
AfeI AGC'GCT
Cuts at: 2241 2241
Size: 6007
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 2542 2576 3051 4906 5883 1203
Size: 1339 34 475 1855 977 1327
Fragments arranged by size:
1855 1339 1327 977 475 34
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 4736 4736
Size: 6007
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2099 1184
Size: 915 5092
AlwNI CAG_nnn'CTG
Cuts at: 2035 2372 2402 2420 2969 3073 3750 5322 2035
Size: 337 30 18 549 104 677 1572 2720
Fragments arranged by size:
2720 1572 677 549 337 104 30 18
ApaLI G'TGCA_C
Cuts at: 3287 4064 5220 3287
Size: 777 1156 4074
Fragments arranged by size:
4074 1156 777
ApoI r'AATT_y
Cuts at: 1493 2531 2630 3007 3040 3187 3316 3542 3694
Size: 1038 99 377 33 147 129 226 152
Cuts at: 3694 5803 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1038 377 226 152 147 129
99 33
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 3332 1870
Size: 73 1389 4545
Fragments arranged by size:
4545 1389 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3328 4783 618
Size: 325 217 627 1541 1455 1842
Fragments arranged by size:
1842 1541 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2299 3326 1391
Size: 908 1027 4072
Fragments arranged by size:
4072 1027 908
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5036
BbvCI CC'TCA_GC
Cuts at: 2777 2777
Size: 6007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4198 4232 1903
Size: 34 2261 34 3678
Fragments arranged by size:
3678 2261 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2860 3124 3289 3899 5109 2860
Size: 264 165 610 1210 3758
Fragments arranged by size:
3758 1210 610 264 165
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6002 6002
Size: 6007
BlpI GC'TnA_GC
Cuts at: 3424 3424
Size: 6007
Bme1580I G_kGCm'C
Cuts at: 1790 3291 4068 5224 1790
Size: 1501 777 1156 2573
Fragments arranged by size:
2573 1501 1156 777
BmgBI CAC'GTC
Cuts at: 1318 2579 1318
Size: 1261 4746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2787 3738 4696
Size: 416 53 83 399 24 1370 951 958
Cuts at: 4696 442
Size: 1753
Fragments arranged by size:
1753 1370 958 951 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2442 2525 4667 1239
Size: 1203 83 2142 2579
Fragments arranged by size:
2579 2142 1203 83
Bpu10I CC'TnA_GC
Cuts at: 2777 2777
Size: 6007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4132 4997 5295 4132
Size: 865 298 4844
Fragments arranged by size:
4844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2285 4670 882
Size: 617 786 2385 2219
Fragments arranged by size:
2385 2219 786 617
BsaAI yAC'GTr
Cuts at: 493 1204 3798 493
Size: 711 2594 2702
Fragments arranged by size:
2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3814 4196 4831 275
Size: 53 83 186 936 2281 382 635 1451
Fragments arranged by size:
2281 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3011 4440 4838 5112 5259 5670 3011
Size: 1429 398 274 147 411 3348
Fragments arranged by size:
3348 1429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3099 3129 1178
Size: 30 165 30 1696 30 4056
Fragments arranged by size:
4056 1696 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2307 2318 2825 1186
Size: 1121 11 507 4368
Fragments arranged by size:
4368 1121 507 11
BseYI C'CCAG_C
Cuts at: 2322 5210 5706 2322
Size: 2888 496 2623
Fragments arranged by size:
2888 2623 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 2934 2993 1163
Size: 573 351 847 59 4177
Fragments arranged by size:
4177 847 573 351 59
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2940 3457 4218 5246 1540
Size: 275 186 939 517 761 1028 2301
Fragments arranged by size:
2301 1028 939 761 517 275 186
BsiHKAI G_wGCw'C
Cuts at: 3291 4068 4153 5224 3291
Size: 777 85 1071 4074
Fragments arranged by size:
4074 1071 777 85
BsmI GAATG_Cn'
Cuts at: 3518 3611 3518
Size: 93 5914
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2299 3291 3326 4068 4153 5224 1391
Size: 399 509 992 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 992 742 509 399 85 35
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 2163 3057 3896 5626 2163
Size: 894 839 1730 2544
Fragments arranged by size:
2544 1730 894 839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3362 844
Size: 2518 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3894 1394
Size: 429 2071 3507
Fragments arranged by size:
3507 2071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4651 4838 1700
Size: 110 2841 187 2869
Fragments arranged by size:
2869 2841 187 110
BsrGI T'GTAC_A
Cuts at: 96 3195 96
Size: 3099 2908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 4067 5079 4067
Size: 1012 4995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2402 794
Size: 1608 4399
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstYI r'GATC_y
Cuts at: 1972 3337 3704 4089 4106 5558 5644 5656 6002
Size: 1365 367 385 17 1452 86 12 346
Cuts at: 6002 1972
Size: 1977
Fragments arranged by size:
1977 1452 1365 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2409 513
Size: 552 939 405 4111
Fragments arranged by size:
4111 939 552 405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 3523 4317 4337 3523
Size: 794 20 5193
Fragments arranged by size:
5193 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3213 3311 3213
Size: 98 5909
DraIII CAC_nnn'GTG
Cuts at: 2065 2065
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2056 2101
Size: 54 1425 50 275 18 207 19 45
Cuts at: 2101 2817 2937 3454 4343 5972 8
Size: 716 120 517 889 1629 43
Fragments arranged by size:
1629 1425 889 716 517 275 207 120
54 50 45 43 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2937 3454 1537
Size: 275 1125 517 4090
Fragments arranged by size:
4090 1125 517 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 3937 1353
Size: 896 1688 3423
Fragments arranged by size:
3423 1688 896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2129 4594 4968 5114 387
Size: 1064 138 540 2465 374 146 1280
Fragments arranged by size:
2465 1280 1064 540 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2230 2415 2442 2525 2713 4070 4667
Size: 393 598 185 27 83 188 1357 597
Cuts at: 4667 5454 5722 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1357 787 598 597 393 268 188
185 83 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2376 3270 2376
Size: 894 5113
EcoO109I rG'GnC_Cy
Cuts at: 2308 2369 3096 2308
Size: 61 727 5219
Fragments arranged by size:
5219 727 61
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 2243 5154 1678
Size: 565 2911 2531
Fragments arranged by size:
2911 2531 565
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 4648 4680 4722
Size: 32 135 32 428 32 2616 32 42
Cuts at: 4722 4754 1373
Size: 32 2626
Fragments arranged by size:
2626 2616 428 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3351 3597 669
Size: 988 281 1413 246 3079
Fragments arranged by size:
3079 1413 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 2854 3606 2854
Size: 752 5255
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3085 5121 5305 1305
Size: 1780 2036 184 2007
Fragments arranged by size:
2036 2007 1780 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2058 2819 10
Size: 54 1425 343 226 761 3198
Fragments arranged by size:
3198 1425 761 343 226 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 2837 3056 3062 3965 4324 4483
Size: 648 1479 192 219 6 903 359 159
Cuts at: 4483 518
Size: 2042
Fragments arranged by size:
2042 1479 903 648 359 219 192 159
6
MspA1I CmG'CkG
Cuts at: 1606 2402 2411 2948 2969 3423 4100 5248 5493
Size: 796 9 537 21 454 677 1148 245
Cuts at: 5493 1606
Size: 2120
Fragments arranged by size:
2120 1148 796 677 537 454 245 21
9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4516
Fragments arranged by size:
4516 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 2213 2526 2546 3055 3067 3365 4910
Size: 97 984 313 20 509 12 298 1545
Cuts at: 4910 1132
Size: 2229
Fragments arranged by size:
2229 1545 984 509 313 298 97 20
12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 2542 3051 4906 2542
Size: 509 1855 3643
Fragments arranged by size:
3643 1855 509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 3044 1108
Size: 1077 859 4071
Fragments arranged by size:
4071 1077 859
PfoI T'CCnGG_A
Cuts at: 2018 2018
Size: 6007
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2308 2369 3096 2308
Size: 61 727 5219
Fragments arranged by size:
5219 727 61
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 3577 3577
Size: 6007
PstI C_TGCA'G
Cuts at: 830 2031 2407 3359 830
Size: 1201 376 952 3478
Fragments arranged by size:
3478 1201 952 376
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2402 2948 2969 2402
Size: 546 21 5440
Fragments arranged by size:
5440 546 21
SacII CC_GC'GG
Cuts at: 2412 2412
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SanDI GG'GwC_CC
Cuts at: 3096 3096
Size: 6007
SapI GCTCTTCn'nnn_
Cuts at: 2249 2249
Size: 6007
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SfcI C'TryA_G
Cuts at: 826 1045 2027 2403 3166 3267 3355 4490 5171
Size: 219 982 376 763 101 88 1135 681
Cuts at: 5171 5362 826
Size: 191 1471
Fragments arranged by size:
1471 1135 982 763 681 376 219 191
101 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4111 5012 5274 820
Size: 197 926 2168 901 262 1553
Fragments arranged by size:
2168 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 2762 3931 5
Size: 47 2710 1169 2081
Fragments arranged by size:
2710 2081 1169 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6007
StyI C'CwwG_G
Cuts at: 513 1065 2004 3091 3178 3446 513
Size: 552 939 1087 87 268 3074
Fragments arranged by size:
3074 1087 939 552 268 87
SwaI ATTT'AAAT
Cuts at: 3213 3213
Size: 6007
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4034 4051 4204 4389 4834 325
Size: 1255 2454 17 153 185 445 1498
Fragments arranged by size:
2454 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3195 4253 5965
Size: 274 80 33 51 1443 1218 1058 1712
Cuts at: 5965 96
Size: 138
Fragments arranged by size:
1712 1443 1218 1058 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3099 3131 5967 5999 3099
Size: 32 2836 32 3107
Fragments arranged by size:
3107 2836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6007
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2812 2812
Size: 6007
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SexAI SfiI SfoI SrfI