Restriction map for
pFN21AB7775
(Circular) (Six-base) MAPSORT of: pFN21AB7775.seq Check: 9958 from: 1 to: 6064
pFN21AB7775 6064 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3407 1937
Size: 1470 4594
Acc65I G'GTAC_C
Cuts at: 2562 3385 2562
Size: 823 5241
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2220 3212 4127 5511 5779 1632
Size: 588 992 915 1384 268 1917
Fragments arranged by size:
1917 1384 992 915 588 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 4963 5940 1203
Size: 3760 977 1327
Fragments arranged by size:
3760 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 3807 5379 3807
Size: 1572 4492
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 2156 2466 2721 3373 3599 3751 5860 1493
Size: 663 310 255 652 226 152 2109 1697
Fragments arranged by size:
2109 1697 663 652 310 255 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 3389 1870
Size: 73 1446 4545
Fragments arranged by size:
4545 1446 73
BamHI G'GATC_C
Cuts at: 3208 3394 3761 3208
Size: 186 367 5511
Fragments arranged by size:
5511 367 186
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 2562 3385 4840 618
Size: 325 217 627 244 531 823 1455 1842
Fragments arranged by size:
1842 1455 823 627 531 325 244 217
BanII G_rGCy'C
Cuts at: 1391 3383 1391
Size: 1992 4072
BbeI G_GCGC'C
Cuts at: 2035 2035
Size: 6064
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2923 928
Size: 971 1024 4069
Fragments arranged by size:
4069 1024 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3074 3956 5166 3074
Size: 882 1210 3972
Fragments arranged by size:
3972 1210 882
BclI T'GATC_A
Cuts at: 1548 2355 1548
Size: 807 5257
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4675 136
Size: 107 122 71 631 605 3003 1525
Fragments arranged by size:
3003 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 4125 5281 1790
Size: 2335 1156 2573
Fragments arranged by size:
2573 2335 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3795 4753 442
Size: 416 53 83 399 24 2378 958 1753
Fragments arranged by size:
2378 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2415 4724 1239
Size: 1176 2309 2579
Fragments arranged by size:
2579 2309 1176
Bpu10I CC'TnA_GC
Cuts at: 2768 2768
Size: 6064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4189 5054 5352 4189
Size: 865 298 4901
Fragments arranged by size:
4901 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4727 882
Size: 617 3228 2219
Fragments arranged by size:
3228 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3855 493
Size: 711 2651 2702
Fragments arranged by size:
2702 2651 711
BsaBI GATnn'nnATC
Cuts at: 2865 6058 2865
Size: 3193 2871
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2032 2042 3871 4253
Size: 53 83 186 936 499 10 1829 382
Cuts at: 4253 4888 275
Size: 635 1451
Fragments arranged by size:
1829 1451 936 635 499 382 186 83
53 10
BsaWI w'CCGG_w
Cuts at: 4497 4895 5169 5316 5727 4497
Size: 398 274 147 411 4834
Fragments arranged by size:
4834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3246 3276 1178
Size: 30 165 30 1843 30 3966
Fragments arranged by size:
3966 1843 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6064
BseYI C'CCAG_C
Cuts at: 2552 5267 5763 2552
Size: 2715 496 2853
Fragments arranged by size:
2853 2715 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4125 4210 5281 4125
Size: 85 1071 4908
Fragments arranged by size:
4908 1071 85
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3383 4125 4210 5281 1391
Size: 399 1593 742 85 1071 2174
Fragments arranged by size:
2174 1593 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 2496 3953 5683 2496
Size: 1457 1730 2877
Fragments arranged by size:
2877 1730 1457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3040 3419 844
Size: 2196 379 3489
Fragments arranged by size:
3489 2196 379
BsrBI CCG'CTC
Cuts at: 1394 1823 3951 1394
Size: 429 2128 3507
Fragments arranged by size:
3507 2128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2663 96
Size: 2567 3497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6064
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 2695 3151 3208 3394 3761 4146 4163 5615
Size: 723 456 57 186 367 385 17 1452
Cuts at: 5615 5701 5713 6059 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 723 456 385 367 346 186
86 57 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3142 513
Size: 552 939 1138 3435
Fragments arranged by size:
3435 1138 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2366 487
Size: 683 56 1140 4185
Fragments arranged by size:
4185 1140 683 56
BtsI GCAGTG_nn'
Cuts at: 3580 4374 4394 3580
Size: 794 20 5250
Fragments arranged by size:
5250 794 20
ClaI AT'CG_AT
Cuts at: 1554 3279 1554
Size: 1725 4339
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2842 3511 4400
Size: 54 1425 50 275 18 1012 669 889
Cuts at: 4400 6029 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1012 889 669 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3231 3994 1353
Size: 1878 763 3423
Fragments arranged by size:
3423 1878 763
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4651 5025 5171 387
Size: 1064 138 3062 374 146 1280
Fragments arranged by size:
3062 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 2415 3212 4127 4724 5511 5779
Size: 393 588 195 797 915 597 787 268
Cuts at: 5779 1239
Size: 1524
Fragments arranged by size:
1524 915 797 787 597 588 393 268
195
EcoRI G'AATT_C
Cuts at: 2156 3373 3751 2156
Size: 1217 378 4469
Fragments arranged by size:
4469 1217 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2039 2528 2701 3010 2039
Size: 489 173 309 5093
Fragments arranged by size:
5093 489 309 173
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 2035 5211 1678
Size: 357 3176 2531
Fragments arranged by size:
3176 2531 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2768 2800 4705 4737 4779
Size: 32 135 32 1196 32 1905 32 42
Cuts at: 4779 4811 1373
Size: 32 2626
Fragments arranged by size:
2626 1905 1196 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3654 669
Size: 988 281 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KasI G'GCGC_C
Cuts at: 2031 2031
Size: 6064
KpnI G_GTAC'C
Cuts at: 2566 3389 2566
Size: 823 5241
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5178 5362 1305
Size: 3873 184 2007
Fragments arranged by size:
3873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2844 10
Size: 54 1425 343 1012 3230
Fragments arranged by size:
3230 1425 1012 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4022 4381 4540 518
Size: 648 2856 359 159 2042
Fragments arranged by size:
2856 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3480 4157 5305 5550 1606
Size: 1874 677 1148 245 2120
Fragments arranged by size:
2120 1874 1148 677 245
NarI GG'CG_CC
Cuts at: 2032 2032
Size: 6064
NcoI C'CATG_G
Cuts at: 513 1065 2004 3142 513
Size: 552 939 1138 3435
Fragments arranged by size:
3435 1138 939 552
NdeI CA'TA_TG
Cuts at: 387 2458 387
Size: 2071 3993
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2742 2881 3422 4967 1132
Size: 97 1513 139 541 1545 2229
Fragments arranged by size:
2229 1545 1513 541 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5162
PfoI T'CCnGG_A
Cuts at: 2949 2949
Size: 6064
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2927 1613
Size: 1314 4750
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 6032
PstI C_TGCA'G
Cuts at: 830 3037 3349 3416 830
Size: 2207 312 67 3478
Fragments arranged by size:
3478 2207 312 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 2849 4312 6024 2849
Size: 1463 1712 2889
Fragments arranged by size:
2889 1712 1463
SfcI C'TryA_G
Cuts at: 826 1045 2216 3033 3345 3412 4547 5228 5419
Size: 219 1171 817 312 67 1135 681 191
Cuts at: 5419 826
Size: 1471
Fragments arranged by size:
1471 1171 1135 817 681 312 219 191
67
SfoI GGC'GCC
Cuts at: 2033 2033
Size: 6064
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4168 5069 5331 820
Size: 197 926 2225 901 262 1553
Fragments arranged by size:
2225 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 2742 2881 3422 1132
Size: 1610 139 541 3774
Fragments arranged by size:
3774 1610 541 139
SspI AAT'ATT
Cuts at: 5 52 2117 3988 5
Size: 47 2065 1871 2081
Fragments arranged by size:
2081 2065 1871 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6064
StyI C'CwwG_G
Cuts at: 513 1065 2004 2787 3142 3503 513
Size: 552 939 783 355 361 3074
Fragments arranged by size:
3074 939 783 552 361 355
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4091 4108 4261 4446 4891 325
Size: 1255 2511 17 153 185 445 1498
Fragments arranged by size:
2511 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2663 2847 4310
Size: 274 80 33 51 1443 686 184 1463
Cuts at: 4310 6022 96
Size: 1712 138
Fragments arranged by size:
1712 1463 1443 686 274 184 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6024 6056 6024
Size: 32 6032
Tth111I GACn'n_nGTC
Cuts at: 1316 3002 1316
Size: 1686 4378
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6064
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 2022 2467 2681 3290 4193 2022
Size: 445 214 609 903 3893
Fragments arranged by size:
3893 903 609 445 214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI