Restriction map for
pFN21AB7772
(Circular) (Six-base) MAPSORT of: pFN21AB7772.seq Check: 8240 from: 1 to: 6220
pFN21AB7772 6220 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3147 4030 278
Size: 53 83 186 936 1611 883 2468
Fragments arranged by size:
2468 1611 936 883 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2850 3399 4283 5667 5935 1632
Size: 1218 549 884 1384 268 1917
Fragments arranged by size:
1917 1384 1218 884 549 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 5119 6096 1203
Size: 3916 977 1327
Fragments arranged by size:
3916 1327 977
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2630 1184
Size: 1446 4774
AlwNI CAG_nnn'CTG
Cuts at: 2039 2837 3100 3963 5535 2039
Size: 798 263 863 1572 2724
Fragments arranged by size:
2724 1572 863 798 263
ApaI G_GGCC'C
Cuts at: 2243 3060 2243
Size: 817 5403
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2023 3529 3755 3907 6016 1493
Size: 530 1506 226 152 2109 1697
Fragments arranged by size:
2109 1697 1506 530 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2147 3545 1870
Size: 73 204 1398 4545
Fragments arranged by size:
4545 1398 204 73
BamHI G'GATC_C
Cuts at: 2008 3550 3917 2008
Size: 1542 367 4311
Fragments arranged by size:
4311 1542 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2678 3541 4996 618
Size: 325 217 627 891 863 1455 1842
Fragments arranged by size:
1842 1455 891 863 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2243 2696 2934 3060 3539 1391
Size: 852 453 238 126 479 4072
Fragments arranged by size:
4072 852 479 453 238 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2191 2225 4411 4445 1903
Size: 34 254 34 2186 34 3678
Fragments arranged by size:
3678 2186 254 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 2218 2700
Size: 107 122 71 631 605 364 182 482
Cuts at: 2700 4831 136
Size: 2131 1525
Fragments arranged by size:
2131 1525 631 605 482 364 182 122
107 71
BglII A'GATC_T
Cuts at: 2161 2382 3415 6215 2161
Size: 221 1033 2800 2166
Fragments arranged by size:
2800 2166 1033 221
BlpI GC'TnA_GC
Cuts at: 2443 3637 2443
Size: 1194 5026
Bme1580I G_kGCm'C
Cuts at: 1790 2243 3060 3334 4281 5437 1790
Size: 453 817 274 947 1156 2573
Fragments arranged by size:
2573 1156 947 817 453 274
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3951 4909 442
Size: 416 53 83 399 24 2534 958 1753
Fragments arranged by size:
2534 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3070 3122 3202 3252 3300 3461 4880 1239
Size: 1831 52 80 50 48 161 1419 2579
Fragments arranged by size:
2579 1831 1419 161 80 52 50 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2845 4345 5210 5508 2845
Size: 1500 865 298 3557
Fragments arranged by size:
3557 1500 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2978 4883 882
Size: 617 1479 1905 2219
Fragments arranged by size:
2219 1905 1479 617
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 3414 6214 3414
Size: 2800 3420
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3144 3237 4027 4409
Size: 53 83 186 936 1611 93 790 382
Cuts at: 4409 5044 275
Size: 635 1451
Fragments arranged by size:
1611 1451 936 790 635 382 186 93
83 53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3017 3047 1178
Size: 30 165 30 1614 30 4351
Fragments arranged by size:
4351 1614 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6220
BseYI C'CCAG_C
Cuts at: 2244 3046 3199 3275 5423 5919 2244
Size: 802 153 76 2148 496 2545
Fragments arranged by size:
2545 2148 802 496 153 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2080 2468 1163
Size: 573 344 388 4915
Fragments arranged by size:
4915 573 388 344
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2235 3670 4431 5459 1540
Size: 275 186 234 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 234 186
BsiHKAI G_wGCw'C
Cuts at: 3366 4281 4366 5437 3366
Size: 915 85 1071 4149
Fragments arranged by size:
4149 1071 915 85
BsmI GAATG_Cn'
Cuts at: 2305 3405 3731 3824 2305
Size: 1100 326 93 4701
Fragments arranged by size:
4701 1100 326 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2243 2696 2934 3060 3334 3366 3539
Size: 399 453 453 238 126 274 32 173
Cuts at: 3539 4281 4366 5437 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 453 453 399 274 238
173 126 85 32
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 3150 4109 5839 3150
Size: 959 1730 3531
Fragments arranged by size:
3531 1730 959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3575 844
Size: 2731 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2091 2565 4107 1394
Size: 429 268 474 1542 3507
Fragments arranged by size:
3507 1542 474 429 268
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2230 2519 3008 4864 5051 1700
Size: 110 420 289 489 1856 187 2869
Fragments arranged by size:
2869 1856 489 420 289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2730 794
Size: 1936 4284
BstBI TT'CG_AA
Cuts at: 2070 5024 2070
Size: 2954 3266
BstEII G'GTnAC_C
Cuts at: 1184 3004 1184
Size: 1820 4400
BstXI CCAn_nnnn'nTGG
Cuts at: 3226 3283 3349 3226
Size: 57 66 6097
Fragments arranged by size:
6097 66 57
BstYI r'GATC_y
Cuts at: 1972 2008 2059 2161 2382 3163 3409 3415 3514
Size: 36 51 102 221 781 246 6 99
Cuts at: 3514 3550 3917 4302 4319 5771 5857 5869 6215
Size: 36 367 385 17 1452 86 12 346
Cuts at: 6215 1972
Size: 1977
Fragments arranged by size:
1977 1452 781 385 367 346 246 221
102 99 86 51 36 36 17 12
6
Bsu36I CC'TnA_GG
Cuts at: 2550 3405 2550
Size: 855 5365
BtgI C'CryG_G
Cuts at: 513 1065 2004 2595 3231 513
Size: 552 939 591 636 3502
Fragments arranged by size:
3502 939 636 591 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2806 3736 4530 4550 2806
Size: 930 794 20 4476
Fragments arranged by size:
4476 930 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2701 3228
Size: 54 1425 50 275 18 402 469 527
Cuts at: 3228 3667 4556 6185 8
Size: 439 889 1629 43
Fragments arranged by size:
1629 1425 889 527 469 439 402 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2232 3667 1537
Size: 275 420 1435 4090
Fragments arranged by size:
4090 1435 420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2950 3195 4150 1353
Size: 1597 245 955 3423
Fragments arranged by size:
3423 1597 955 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2456 2864 4807 5181 5327 387
Size: 1064 138 867 408 1943 374 146 1280
Fragments arranged by size:
1943 1280 1064 867 408 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2850 3070 3122 3202 3252 3300 3399
Size: 393 1218 220 52 80 50 48 99
Cuts at: 3399 3461 4283 4880 5667 5935 1239
Size: 62 822 597 787 268 1524
Fragments arranged by size:
1524 1218 822 787 597 393 268 220
99 80 62 52 50 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2553 2553
Size: 6220
EcoO109I rG'GnC_Cy
Cuts at: 2239 2240 2273 3056 3057 3106 3272 2239
Size: 1 33 783 1 49 166 5187
Fragments arranged by size:
5187 783 166 49 33 1 1
EcoRI G'AATT_C
Cuts at: 2023 3529 3907 2023
Size: 1506 378 4336
Fragments arranged by size:
4336 1506 378
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2194 2355 5367 1678
Size: 516 161 3012 2531
Fragments arranged by size:
3012 2531 516 161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2106 2138 4861 4893 4935
Size: 32 135 32 534 32 2723 32 42
Cuts at: 4935 4967 1373
Size: 32 2626
Fragments arranged by size:
2723 2626 534 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2711 3209 5334 5518 1305
Size: 1406 498 2125 184 2007
Fragments arranged by size:
2125 2007 1406 498 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3230 10
Size: 54 1425 343 1398 3000
Fragments arranged by size:
3000 1425 1398 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4178 4537 4696 518
Size: 648 3012 359 159 2042
Fragments arranged by size:
3012 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3636 4313 5461 5706 1606
Size: 2030 677 1148 245 2120
Fragments arranged by size:
2120 2030 1148 677 245
NaeI GCC'GGC
Cuts at: 2232 2232
Size: 6220
NcoI C'CATG_G
Cuts at: 513 1065 2004 2595 513
Size: 552 939 591 4138
Fragments arranged by size:
4138 939 591 552
NdeI CA'TA_TG
Cuts at: 387 2320 387
Size: 1933 4287
NgoMIV G'CCGG_C
Cuts at: 2230 2230
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 3578 5123 1132
Size: 97 2349 1545 2229
Fragments arranged by size:
2349 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6220
PciI A'CATG_T
Cuts at: 5119 5119
Size: 6220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3100 1108
Size: 1992 4228
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2273 2273
Size: 6220
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2239 3056 2239
Size: 817 5403
PstI C_TGCA'G
Cuts at: 830 3572 830
Size: 2742 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 3025 3568 4703 5384 5575 826
Size: 219 1980 543 1135 681 191 1471
Fragments arranged by size:
1980 1471 1135 681 543 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2824 4324 5225 5487 820
Size: 197 926 881 1500 901 262 1553
Fragments arranged by size:
1553 1500 926 901 881 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 2004 2595 3342 3659 513
Size: 552 939 591 747 317 3074
Fragments arranged by size:
3074 939 747 591 552 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4247 4264 4417 4602 5047 325
Size: 1255 2667 17 153 185 445 1498
Fragments arranged by size:
2667 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4466 6178 96
Size: 274 80 33 51 1443 2489 1712 138
Fragments arranged by size:
2489 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2462 2494 2758 2790 6180 6212 2462
Size: 32 264 32 3390 32 2470
Fragments arranged by size:
3390 2470 264 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 2914 3412 3556 2914
Size: 498 144 5578
Fragments arranged by size:
5578 498 144
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 2785 4349 2785
Size: 1564 4656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3145 4028 276
Size: 53 83 186 936 1611 883 2468
Fragments arranged by size:
2468 1611 936 883 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI