Restriction map for
pFN21AB7771
(Circular) (Six-base) MAPSORT of: pFN21AB7771.seq Check: 4725 from: 1 to: 6055
pFN21AB7771 6055 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 1937
Size: 1461 4594
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2808 2946 4118 5502 5770 1632
Size: 1176 138 1172 1384 268 1917
Fragments arranged by size:
1917 1384 1176 1172 268 138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 2191 4954 5931 1203
Size: 988 2763 977 1327
Fragments arranged by size:
2763 1327 988 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 3076 4784 3076
Size: 1708 4347
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AlwNI CAG_nnn'CTG
Cuts at: 3798 5370 3798
Size: 1572 4483
ApaLI G'TGCA_C
Cuts at: 4112 5268 4112
Size: 1156 4899
ApoI r'AATT_y
Cuts at: 1493 2288 2636 3364 3590 3742 5851 1493
Size: 795 348 728 226 152 2109 1697
Fragments arranged by size:
2109 1697 795 728 348 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 3380 1870
Size: 73 1437 4545
Fragments arranged by size:
4545 1437 73
BamHI G'GATC_C
Cuts at: 3385 3752 3385
Size: 367 5688
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3376 4831 618
Size: 325 217 627 1589 1455 1842
Fragments arranged by size:
1842 1589 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2311 3374 1391
Size: 920 1063 4072
Fragments arranged by size:
4072 1063 920
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5084
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4246 4280 1903
Size: 34 2309 34 3678
Fragments arranged by size:
3678 2309 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3947 5157 3947
Size: 1210 4845
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4666 136
Size: 107 122 71 631 605 2994 1525
Fragments arranged by size:
2994 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 3226 4116 5272 1790
Size: 1436 890 1156 2573
Fragments arranged by size:
2573 1436 1156 890
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2570 3786 4744
Size: 416 53 83 399 24 1153 1216 958
Cuts at: 4744 442
Size: 1753
Fragments arranged by size:
1753 1216 1153 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2717 4715 1239
Size: 1478 1998 2579
Fragments arranged by size:
2579 1998 1478
Bpu10I CC'TnA_GC
Cuts at: 2485 2485
Size: 6055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4180 5045 5343 4180
Size: 865 298 4892
Fragments arranged by size:
4892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2270 4718 882
Size: 617 771 2448 2219
Fragments arranged by size:
2448 2219 771 617
BsaAI yAC'GTr
Cuts at: 493 1204 3846 493
Size: 711 2642 2702
Fragments arranged by size:
2702 2642 711
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3862 4244 4879 275
Size: 53 83 186 936 2329 382 635 1451
Fragments arranged by size:
2329 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4488 4886 5160 5307 5718 4488
Size: 398 274 147 411 4825
Fragments arranged by size:
4825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3169 3199 1178
Size: 30 165 30 1766 30 4034
Fragments arranged by size:
4034 1766 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2785 2788 2791 2794 3309 1186
Size: 1599 3 3 3 515 3932
Fragments arranged by size:
3932 1599 515 3 3 3
BseYI C'CCAG_C
Cuts at: 5258 5754 5258
Size: 496 5559
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5482
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3505 4266 5294 1540
Size: 275 186 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3318 4116 4201 5272 3318
Size: 798 85 1071 4101
Fragments arranged by size:
4101 1071 798 85
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2311 3226 3318 3374 4116 4201 5272
Size: 399 521 915 92 56 742 85 1071
Cuts at: 5272 1391
Size: 2174
Fragments arranged by size:
2174 1071 915 742 521 399 92 85
56
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 2959 3944 5674 2959
Size: 985 1730 3340
Fragments arranged by size:
3340 1730 985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3410 844
Size: 2566 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3942 1394
Size: 429 2119 3507
Fragments arranged by size:
3507 2119 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4550 4724 65
Size: 1183 3302 174 1396
Fragments arranged by size:
3302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4699 4886 1700
Size: 110 2889 187 2869
Fragments arranged by size:
2889 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3306 96
Size: 3210 2845
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 2313 3026 4115 5127 2313
Size: 713 1089 1012 3241
Fragments arranged by size:
3241 1089 1012 713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 2374 4859 2374
Size: 2485 3570
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 2623 3385 3752 4137 4154 5606 5692 5704
Size: 651 762 367 385 17 1452 86 12
Cuts at: 5704 6050 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 762 651 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3145 3274 513
Size: 552 939 1141 129 3294
Fragments arranged by size:
3294 1141 939 552 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 3571 4365 4385 3571
Size: 794 20 5241
Fragments arranged by size:
5241 794 20
ClaI AT'CG_AT
Cuts at: 1554 2495 1554
Size: 941 5114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 2643 3359 2643
Size: 716 5339
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3502 4391 6020
Size: 54 1425 50 275 18 1672 889 1629
Cuts at: 6020 8
Size: 43
Fragments arranged by size:
1672 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3502 1537
Size: 275 1690 4090
Fragments arranged by size:
4090 1690 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2738 2782 2812 2922 2942 3101 3237 3985
Size: 1385 44 30 110 20 159 136 748
Cuts at: 3985 1353
Size: 3423
Fragments arranged by size:
3423 1385 748 159 136 110 44 30
20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4642 5016 5162 387
Size: 1064 138 3053 374 146 1280
Fragments arranged by size:
3053 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2717 2808 2946 4118 4715 5502 5770
Size: 393 1085 91 138 1172 597 787 268
Cuts at: 5770 1239
Size: 1524
Fragments arranged by size:
1524 1172 1085 787 597 393 268 138
91
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 6055
FspI TGC'GCA
Cuts at: 4561 4561
Size: 6055
HaeII r_GCGC'y
Cuts at: 1678 5202 1678
Size: 3524 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3062 3094 3169 3201 4696
Size: 32 135 32 1490 32 75 32 1495
Cuts at: 4696 4728 4770 4802 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1495 1490 135 75 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2416 3399 3645 669
Size: 988 281 478 983 246 3079
Fragments arranged by size:
3079 988 983 478 281 246
HindIII A'AGCT_T
Cuts at: 748 2954 748
Size: 2206 3849
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5169 5353 1305
Size: 3864 184 2007
Fragments arranged by size:
3864 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4233
Fragments arranged by size:
4233 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4013 4372 4531 518
Size: 648 2847 359 159 2042
Fragments arranged by size:
2847 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3471 4148 5296 5541 1606
Size: 1865 677 1148 245 2120
Fragments arranged by size:
2120 1865 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3274 513
Size: 552 939 1270 3294
Fragments arranged by size:
3294 1270 939 552
NdeI CA'TA_TG
Cuts at: 387 2104 2161 387
Size: 1717 57 4281
Fragments arranged by size:
4281 1717 57
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 3413 4958 1132
Size: 97 2184 1545 2229
Fragments arranged by size:
2229 2184 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6055
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6055
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4124 4156 5637 5669 4124
Size: 32 1481 32 4510
Fragments arranged by size:
4510 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PstI C_TGCA'G
Cuts at: 830 3407 830
Size: 2577 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SapI GCTCTTCn'nnn_
Cuts at: 2738 3237 2738
Size: 499 5556
SbfI CC_TGCA'GG
Cuts at: 3407 3407
Size: 6055
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SfcI C'TryA_G
Cuts at: 826 1045 3403 4538 5219 5410 826
Size: 219 2358 1135 681 191 1471
Fragments arranged by size:
2358 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 3382 1872
Size: 1510 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4159 5060 5322 820
Size: 197 926 2216 901 262 1553
Fragments arranged by size:
2216 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6055
StyI C'CwwG_G
Cuts at: 513 1065 2004 3000 3274 3494 513
Size: 552 939 996 274 220 3074
Fragments arranged by size:
3074 996 939 552 274 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2294 3333 4082 4099 4252 4437 4882
Size: 1255 714 1039 749 17 153 185 445
Cuts at: 4882 325
Size: 1498
Fragments arranged by size:
1498 1255 1039 749 714 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2557 3306 4301
Size: 274 80 33 51 1443 580 749 995
Cuts at: 4301 6013 96
Size: 1712 138
Fragments arranged by size:
1712 1443 995 749 580 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6015 6047 6015
Size: 32 6023
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6055
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6055
XmaI C'CCGG_G
Cuts at: 1870 3380 1870
Size: 1510 4545
XmnI GAAnn'nnTTC
Cuts at: 2015 4184 2015
Size: 2169 3886
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI