Restriction map for
pFN21AB7770
(Circular) (Six-base) MAPSORT of: pFN21AB7770.seq Check: 8091 from: 1 to: 6136
pFN21AB7770 6136 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3355 3355
Size: 6136
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 3457 3457
Size: 6136
AclI AA'CG_TT
Cuts at: 1563 4263 4636 1563
Size: 2700 373 3063
Fragments arranged by size:
3063 2700 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3287 4199 5583 5851 1632
Size: 1655 912 1384 268 1917
Fragments arranged by size:
1917 1655 1384 912 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5939
AflIII A'CryG_T
Cuts at: 1203 3349 5035 6012 1203
Size: 2146 1686 977 1327
Fragments arranged by size:
2146 1686 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 3338 4865 3338
Size: 1527 4609
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6136
AlwNI CAG_nnn'CTG
Cuts at: 3361 3879 5451 3361
Size: 518 1572 4046
Fragments arranged by size:
4046 1572 518
ApaI G_GGCC'C
Cuts at: 2644 2644
Size: 6136
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 2761 3445 3671 3823 5932 1493
Size: 1268 684 226 152 2109 1697
Fragments arranged by size:
2109 1697 1268 684 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4690 160
Size: 872 3658 1606
Fragments arranged by size:
3658 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 3461 1870
Size: 73 1518 4545
Fragments arranged by size:
4545 1518 73
BamHI G'GATC_C
Cuts at: 2463 3466 3833 2463
Size: 1003 367 4766
Fragments arranged by size:
4766 1003 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3457 4912 618
Size: 325 217 627 1670 1455 1842
Fragments arranged by size:
1842 1670 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2644 3102 3261 3455 1391
Size: 1253 458 159 194 4072
Fragments arranged by size:
4072 1253 458 194 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 2163 2282 3231 3383 3389 928
Size: 971 189 75 119 949 152 6 3675
Fragments arranged by size:
3675 971 949 189 152 119 75 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4327 4361 1903
Size: 34 2390 34 3678
Fragments arranged by size:
3678 2390 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 3369 1548
Size: 1821 4315
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 4747 136
Size: 107 122 71 631 605 363 2712 1525
Fragments arranged by size:
2712 1525 631 605 363 122 107 71
BglII A'GATC_T
Cuts at: 2592 3417 6131 2592
Size: 825 2714 2597
Fragments arranged by size:
2714 2597 825
BlpI GC'TnA_GC
Cuts at: 3553 3553
Size: 6136
Bme1580I G_kGCm'C
Cuts at: 1790 2644 4197 5353 1790
Size: 854 1553 1156 2573
Fragments arranged by size:
2573 1553 1156 854
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6136
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3867 4825 442
Size: 416 53 83 399 24 2450 958 1753
Fragments arranged by size:
2450 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2116 2581 3245 3275 3296 4796 1239
Size: 877 465 664 30 21 1500 2579
Fragments arranged by size:
2579 1500 877 664 465 30 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4261 5126 5424 4261
Size: 865 298 4973
Fragments arranged by size:
4973 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3183 4799 882
Size: 617 1684 1616 2219
Fragments arranged by size:
2219 1684 1616 617
BsaAI yAC'GTr
Cuts at: 493 1204 3352 3927 493
Size: 711 2148 575 2702
Fragments arranged by size:
2702 2148 711 575
BsaBI GATnn'nnATC
Cuts at: 6130 6130
Size: 6136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3943 4325 4960 275
Size: 53 83 186 936 2410 382 635 1451
Fragments arranged by size:
2410 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2409 4569 4967 5241 5388 5799 2409
Size: 2160 398 274 147 411 2746
Fragments arranged by size:
2746 2160 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 2567 2597 3089
Size: 30 165 30 675 30 459 30 492
Cuts at: 3089 3119 1178
Size: 30 4195
Fragments arranged by size:
4195 675 492 459 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2945 1186
Size: 1759 4377
BseYI C'CCAG_C
Cuts at: 5339 5835 5339
Size: 496 5640
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2903 3374 1163
Size: 573 1167 471 3925
Fragments arranged by size:
3925 1167 573 471
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3586 4347 5375 1540
Size: 275 186 1585 761 1028 2301
Fragments arranged by size:
2301 1585 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3261 4197 4282 5353 3261
Size: 936 85 1071 4044
Fragments arranged by size:
4044 1071 936 85
BsmI GAATG_Cn'
Cuts at: 2910 3647 3740 2910
Size: 737 93 5306
Fragments arranged by size:
5306 737 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2644 3102 3261 3455 4197 4282 5353
Size: 399 854 458 159 194 742 85 1071
Cuts at: 5353 1391
Size: 2174
Fragments arranged by size:
2174 1071 854 742 458 399 194 159
85
BspEI T'CCGG_A
Cuts at: 2409 5799 2409
Size: 3390 2746
BspHI T'CATG_A
Cuts at: 2299 3045 4025 5755 2299
Size: 746 980 1730 2680
Fragments arranged by size:
2680 1730 980 746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3249 3355 3491 844
Size: 2405 106 136 3489
Fragments arranged by size:
3489 2405 136 106
BsrBI CCG'CTC
Cuts at: 1394 1823 3406 4023 1394
Size: 429 1583 617 3507
Fragments arranged by size:
3507 1583 617 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4631 4805 65
Size: 1183 3383 174 1396
Fragments arranged by size:
3383 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4780 4967 1700
Size: 110 2970 187 2869
Fragments arranged by size:
2970 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6136
BssSI C'ACGA_G
Cuts at: 2972 4196 5208 2972
Size: 1224 1012 3900
Fragments arranged by size:
3900 1224 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3361 794
Size: 2567 3569
BstBI TT'CG_AA
Cuts at: 4940 4940
Size: 6136
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6136
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6136
BstYI r'GATC_y
Cuts at: 1972 2065 2179 2463 2592 3417 3466 3833 4218
Size: 93 114 284 129 825 49 367 385
Cuts at: 4218 4235 5687 5773 5785 6131 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 825 385 367 346 284 129
114 93 86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2010 2061 2871 513
Size: 552 945 51 810 3778
Fragments arranged by size:
3778 945 810 552 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5397
Fragments arranged by size:
5397 683 56
BtsI GCAGTG_nn'
Cuts at: 2526 3652 4446 4466 2526
Size: 1126 794 20 4196
Fragments arranged by size:
4196 1126 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2029 2064 2223 2258 529
Size: 35 1465 35 159 35 4407
Fragments arranged by size:
4407 1465 159 35 35 35
DraI TTT'AAA
Cuts at: 3440 3440
Size: 6136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5143 809
Size: 507 3827 1802
Fragments arranged by size:
3827 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2835 3583 4472
Size: 54 1425 50 275 18 1005 748 889
Cuts at: 4472 6101 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1005 889 748 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3583 1537
Size: 275 1771 4090
Fragments arranged by size:
4090 1771 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2298 4066 1353
Size: 945 1768 3423
Fragments arranged by size:
3423 1768 945
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2569 2603 4723 5097 5243 387
Size: 1064 138 980 34 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 980 374 146 138 34
EcoICRI GAG'CTC
Cuts at: 3259 3259
Size: 6136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2116 2581 3245 3275 3287 3296 4199
Size: 393 484 465 664 30 12 9 903
Cuts at: 4199 4796 5583 5851 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 903 787 664 597 484 465 393
268 30 12 9
EcoO109I rG'GnC_Cy
Cuts at: 2641 2641
Size: 6136
EcoRI G'AATT_C
Cuts at: 2761 3445 3823 2761
Size: 684 378 5074
Fragments arranged by size:
5074 684 378
EcoRV GAT'ATC
Cuts at: 2104 2605 2104
Size: 501 5635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 6136
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 5283 1678
Size: 3605 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 3324 3356 4777
Size: 32 135 32 665 32 1055 32 1421
Cuts at: 4777 4809 4851 4883 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1421 1055 665 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2017 2125 3480 3726 669
Size: 988 281 79 108 1355 246 3079
Fragments arranged by size:
3079 1355 988 281 246 108 79
HindIII A'AGCT_T
Cuts at: 748 2355 2429 748
Size: 1607 74 4455
Fragments arranged by size:
4455 1607 74
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KpnI G_GTAC'C
Cuts at: 3461 3461
Size: 6136
MfeI C'AATT_G
Cuts at: 2328 3735 2328
Size: 1407 4729
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2337 2441 5250 5434 1305
Size: 1032 104 2809 184 2007
Fragments arranged by size:
2809 2007 1032 184 104
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2837 10
Size: 54 1425 343 1005 3309
Fragments arranged by size:
3309 1425 1005 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2024 2385 2658 4094 4453 4612
Size: 648 843 15 361 273 1436 359 159
Cuts at: 4612 518
Size: 2042
Fragments arranged by size:
2042 1436 843 648 361 359 273 159
15
MspA1I CmG'CkG
Cuts at: 1606 2614 2630 2686 3265 3295 3552 4229 5377
Size: 1008 16 56 579 30 257 677 1148
Cuts at: 5377 5622 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1008 677 579 257 245 56
30 16
NcoI C'CATG_G
Cuts at: 513 1065 2010 513
Size: 552 945 4639
Fragments arranged by size:
4639 945 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 3494 5039 1132
Size: 97 2265 1545 2229
Fragments arranged by size:
2265 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6136
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6136
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PmlI CAC'GTG
Cuts at: 3352 3352
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3393 1613
Size: 1780 4356
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PspOMI G'GGCC_C
Cuts at: 2640 2640
Size: 6136
PstI C_TGCA'G
Cuts at: 830 2817 3366 3414 3488 830
Size: 1987 549 48 74 3478
Fragments arranged by size:
3478 1987 549 74 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
PvuII CAG'CTG
Cuts at: 2614 3265 3295 2614
Size: 651 30 5455
Fragments arranged by size:
5455 651 30
SacI G_AGCT'C
Cuts at: 3261 3261
Size: 6136
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SapI GCTCTTCn'nnn_
Cuts at: 2298 2298
Size: 6136
SbfI CC_TGCA'GG
Cuts at: 2817 3414 3488 2817
Size: 597 74 5465
Fragments arranged by size:
5465 597 74
ScaI AGT'ACT
Cuts at: 2495 4384 6096 2495
Size: 1889 1712 2535
Fragments arranged by size:
2535 1889 1712
SfcI C'TryA_G
Cuts at: 826 1045 2813 3362 3410 3484 4619 5300 5491
Size: 219 1768 549 48 74 1135 681 191
Cuts at: 5491 826
Size: 1471
Fragments arranged by size:
1768 1471 1135 681 549 219 191 74
48
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 3463 1872
Size: 1591 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4240 5141 5403 820
Size: 197 926 2297 901 262 1553
Fragments arranged by size:
2297 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 3162 4060 5
Size: 47 3110 898 2081
Fragments arranged by size:
3110 2081 898 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6136
StyI C'CwwG_G
Cuts at: 513 1065 2010 3037 3575 513
Size: 552 945 1027 538 3074
Fragments arranged by size:
3074 1027 945 552 538
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4163 4180 4333 4518 4963 325
Size: 1255 2583 17 153 185 445 1498
Fragments arranged by size:
2583 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2493 4382 6094
Size: 274 80 33 51 1443 516 1889 1712
Cuts at: 6094 96
Size: 138
Fragments arranged by size:
1889 1712 1443 516 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 3269 3301 6096 6128 2078
Size: 32 1159 32 2795 32 2086
Fragments arranged by size:
2795 2086 1159 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6136
XbaI T'CTAG_A
Cuts at: 3472 3472
Size: 6136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 2320
Size: 6136
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6136
XmaI C'CCGG_G
Cuts at: 1870 3461 1870
Size: 1591 4545
XmnI GAAnn'nnTTC
Cuts at: 2160 3228 4265 2160
Size: 1068 1037 4031
Fragments arranged by size:
4031 1068 1037
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI